ASTM E654-96

Standard Test Method for Effectiveness of Aerosol and Pressurized Spray Insecticides Against Cockroaches

SCOPE
1.1 This test method covers the determination of the effectiveness of aerosol and pressurized spray insecticides against cockroaches.  
1.2 Test data by this test method may also be adequate to support claims for use of the product to control the exposed or accessible stages of silverfish, ants, centipedes, spiders, and certain stored product pests.  
1.3 The values stated in inch-pound units are to be regarded as the standard. The values given in parentheses are for information only.
1.4 This standard does not purport to address all of the safety concerns, if any associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 09-Oct-1996
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents

Relations

• Replaced By: ASTM E654-96(2003) - Standard Test Method for Effectiveness of Aerosol and Pressurized Spray Insecticides Against Cockroaches
• Effective Date: 10-Oct-1996

Standards Content (Sample)

NOTICE: This standard has either been superceded and replaced by a new version or discontinued.
Contact ASTM International (www.astm.org) for the latest information.
Designation: E 654 – 96
Standard Test Method for
Effectiveness of Aerosol and Pressurized Spray Insecticides
Against Cockroaches
This standard is issued under the fixed designation E 654; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope accompany the reactions of living organisms. It should be
employed under the supervision of personnel familiar with the
1.1 This test method covers the determination of the effec-
biological testing of insecticides.
tiveness of aerosol and pressurized spray insecticides against
3.3 In order to measure the effectiveness with reasonable
cockroaches.
tolerance, the test is run in conjunction with the Official Test
1.2 Test data by this test method may also be adequate to
Aerosol II (OTA II) as the standard basis of comparison.
support claims for use of the product to control the exposed or
Another standard that may be used with this test method is the
accessible stages of silverfish, ants, centipedes, spiders, and
Tentative Official Aqueous Pressurized Spray (TOAPS).
certain stored product pests.
1.3 The values stated in inch-pound units are to be regarded
4. Apparatus
as the standard. The values given in parentheses are for
4.1 Offıcial Test Aerosol II (OTA II)/Tentative Offıcial Aque-
information only.
ous Pressurized Spray (TOAPS), to be dispensed from the
1.4 This standard does not purport to address all of the
container in which it is supplied. Ensure that the OTA II/
safety concerns, if any, associated with its use. It is the
TOAPS dispenser meets the specifications designated on its
responsibility of the user of this standard to establish appro-
label.
priate safety and health practices and determine the applica-
4.2 Test Specimen Dispenser—No restrictions are made on
bility of regulatory limitations prior to use.
the dispenser. However, the test results apply only to the
2. Terminology particular test specimen as dispensed from the particular unit
employed.
2.1 Definitions of Terms Specific to This Standard:
4.3 Cockroaches—The test insects shall be healthy, normal,
2.1.1 aerosols—for this method, pressurized formulations
undeformed last nymphal instars or young males of the
generally containing 20 % or less of low-volatile ingredients
German cockroach, (Blattella germanica L). Wild cockroaches
(insecticides, base oils, solvents, etc.) and 80 % or more of
shall not be used.
propellant (fluorinated hydrocarbons, liquefied petroleum
4.3.1 Recently emerged last nymphal instars (for example,
gases, etc.).
those whose pigmentation is not dark) shall not be used for
2.1.2 pressurized sprays—for this method, formulations
testing purposes. It is recommended that the last nymphal-
generally containing more than 20 % low-volatile ingredients
instar stage shall have been attained at least 3 days prior to
(insecticides, base oils, solvents, etc.) and 3 to 80 % propellant
testing.
(fluorinated hydrocarbons, liquefied petroleum gases, com-
4.3.2 Any suitable method permitting the production of
pressed gases, etc.).
large numbers of test insects under controlled temperature and
2.1.3 moribund—pertaining to any insect that shows signs
humidity conditions may be employed. The rearing technique
of life, but is incapable of normal locomotion.
described by Woodbury and Barnhart, which makes good use
3. Significance and Use
of a brood chamber containing adult females from which large
numbers of first instar nymphs may be collected at frequent
3.1 This test method provides a satisfactory means of
intervals, has been successfully used.
determining the relative efficiency of aerosol formulations
4.3.3 All test insects shall be reared under uniform condi-
when applied as direct sprays to cockroaches. It is not designed
tions.
to measure the residual action.
4.4 Rearing Room, of any convenient size, free of strong
3.2 As a biological test, it is subject to the variations that
drafts and maintained at a temperature of 80 6 2°F (27 6 1°C)
and a relative humidity of 506 5 %. This room should be
This test method is under the jurisdiction of ASTM Committee E-35 on
Pesticides and is the direct responsibility of Subcommittee E35.12 on Insect Control
Agents. It was originally developed by the Chemical Specialties Manufacturers Available from the Chemical Specialties Manufacturers Assn. 1001 Connecti-
Association. cut Ave. S.W., Washington, DC 20036.
Current edition approved Oct. 10, 1996. Published December 1996. Originally Woodbury, E. N., and Barnhart, C. S., “Tests on Crawling Insects,” Soap and
published as E 564 – 79. Last previous edition E 654 – 90. Sanitary Chemicals, SSCHA, Vol 15, No. 9, 1939.
Copyright © ASTM, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959, United States.
NOTICE: This standard has either been superceded and replaced by a new version or discontinued.
Contact ASTM International (www.astm.org) for the latest information.
E 654
separate from the testing room and should be ventilated to Make an equal number of replicates for members of any test
minimize odors. series on a given test day.
4.5 Test Room, maintained at a temperature of 80 6 2°F (27
5.4 The dosages shall be approximately the same through-
6 1°C) and a relative humidity of 50 6 5 %, of a convenient
out a given test series and shall be of such magnitude as to
size to permit the operator to conduct the test efficiently.
result in an average of 50 to 75 % of the insects dead and
4.6 Spray Chamber, with walls of solid material, 18 in. (457
moribund at 24 h with the OTA II/TOAPS. Consider average
mm) square, 25 to 30 in. (635 to 762 mm) high, with an open
dosages the same if they agree within 0.2 g.
top and floor.
5.5 Weigh the test dispensers both before and after the
4.6.1 The floor of the chamber shall be covered with ⁄2-in.
spraying of each test group and record the weight of the
(13-mm) mesh, wire hardware cloth, with suitable guides
material used.
fastened to permit the centering of the treatment container in a
definite position with respect to the OTA II/ TOAPS nozzle and
6. Procedure
the test specimen dispenser. The front wall may be a sliding
6.1 Isolate the last instar nymphs in the recovery dishes in
door to permit convenient access to the chamber interior. An
groups of 20 with a suction device, by immobilizing them with
adjustable hinged shelf shall be attached to the outside of the
chilling or with carbon dioxide (CO ) gas or by any other
center of the back upper edge of the chamber. A satisfactory
means that will permit handling the insects without injury.
shelf that will permit the OTA II/TOAPS and test specimen to
6.2 Place a square of paper (see 4.8) in the center of the
be held in a standard position is con
...