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ASTM E1303-95(2000)

(Practice)

Practice for Refractive Index Detectors Used in Liquid Chromatography

Practice for Refractive Index Detectors Used in Liquid Chromatography

SCOPE
1.1 This practice is intended to describe tests used to evaluate the performance and to list certain descriptive specifications of a refractive index (RI) detector used as the detection component of a liquid chromatographic (LC) system.
1.2 This practice is intended to describe the performance of the detector both independent of the chromatographic system (static conditions, without flowing solvent) and with flowing solvent (dynamic conditions).
1.3 The values stated in SI units are to be regarded as the standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
14-May-1995
ICS
17.180.30 - Optical measuring instruments
71.040.50 - Physicochemical methods of analysis
Technical Committee
E13 - Molecular Spectroscopy and Separation Science
Drafting Committee
E13.19 - Separation Science
Current Stage
Ref Project

Relations

Replaces
ASTM E1303-95 - Practice for Refractive Index Detectors Used in Liquid Chromatography
Effective Date
15-May-1995
Replaced By
ASTM E1303-95(2005) - Practice for Refractive Index Detectors Used in Liquid Chromatography
Effective Date
01-Feb-2005

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ASTM E1303-95(2000) - Practice for Refractive Index Detectors Used in Liquid ChromatographyASTM E1303-95(2000) - Practice for Refractive Index Detectors Used in Liquid Chromatography
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ASTM E1303-95(2000) - Practice for Refractive Index Detectors Used in Liquid Chromatography
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E1303–95 (Reapproved 2000)
Practice for
1
Refractive Index Detectors Used in Liquid Chromatography
This standard is issued under the fixed designation E1303; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope described in this practice are sufficiently general so that they
may be adapted for use at whatever conditions may be chosen
1.1 This practice is intended to describe tests used to
for other reasons.
evaluate the performance and to list certain descriptive speci-
fications of a refractive index (RI) detector used as the
4. Noise, Drift, and Flow Sensitivity
detectioncomponentofaliquidchromatographic(LC)system.
4.1 Descriptions of Terms Specific to This Standard:
1.2 This practice is intended to describe the performance of
4.1.1 short term noise—Thisnoiseisthemeanamplitudein
the detector both independent of the chromatographic system
refractive index units (RIU) for random variations of the
(static conditions, without flowing solvent) and with flowing
detector signal having a frequency of one or more cycles per
solvent (dynamic conditions).
min. Short term noise limits the smallest signal detectable by
1.3 The values stated in SI units are to be regarded as the
anRIdetector,limitstheprecisionattainableandsetsthelower
standard.
limitonthedynamicrange.Thisnoisecorrespondstoobserved
1.4 This standard does not purport to address all of the
noise of the RI detector only. (The actual noise of the LC
safety concerns, if any, associated with its use. It is the
system may be larger or smaller than the observed value,
responsibility of the user of this standard to establish appro-
depending upon the method of data collection, or signal
priate safety and health practices and determine the applica-
monitoringofthedetector,sinceobservednoiseisafunctionof
bility of regulatory limitations prior to use.
the frequency, speed of response and the band width of the
2. Referenced Documents recorder or other electronic circuit measuring the detector
signal.)
2.1 ASTM Standards:
4.1.2 long term noise—this noise is the maximum ampli-
E386 Practice for Data Presentation Relating to High-
tude in RIU for random variations of the detector signal with
ResolutionNuclearMagneticResonance(NMR)Spectros-
2
frequencies between 6 and 60 cycles per h (0.1 and 1.0 cycles
copy
per min). It represents noise that may be mistaken for a
3. Significance and Use late-eluting peak.This noise corresponds to the observed noise
only and may not always be present.
3.1 Although it is possible to observe and measure each of
4.1.3 drift—the average slope of the long term noise enve-
several characteristics of a detector under different and unique
lope expressed in RIU per hour as measured over a period of
conditions,itistheintentofthispracticethatacompletesetof
1h.
detector test results should be obtained under the same oper-
4.1.4 static—refers to the noise and drift measured under
ating conditions. It should also be noted that to specify
conditions of no flow.
completely a detector’s capability, its performance should be
4.1.5 dynamic—refers to the noise and drift measured at a
measured at several sets of conditions within the useful range
flow rate of 1.0 mL/min.
of the detector.
4.1.6 flow sensitivity—the rate of change of signal displace-
3.2 Theobjectiveofthispracticeistotestthedetectorunder
ment (in RIU) vs flow rate (in mLper min) resulting from step
specified conditions and in a configuration without an LC
changesinflowratecalculatedat1mLperminasdescribedin
column. This is a separation independent test. In certain
4.3.12.
circumstances it might also be necessary to test the detector in
4.2 Test Conditions:
the separation mode with an LC column in the system, and the
4.2.1 Thesametestsolventmustbeusedinbothsampleand
appropriate concerns are also mentioned. The terms and tests
reference cells. The test solvent used and its purity should be
specified. Water equilibrated with the laboratory atmosphere
1
This practice is under the jurisdiction ofASTM Committee E13 on Molecular
containingminimumimpuritiesisthepreferredtestsolventfor
Spectroscopy and is the direct responsibility of Subcommittee E13.19 on Chroma-
measuring noise and drift. Water for this purpose (preferably
tography.
purifiedbydistillation,deionizationorreverseosmosis)should
Current edition approved May 15, 1995. Published July 1995. Originally
published as E1303–89. Last previous edition E1303–89.
2
Annual Book of ASTM Standards, Vol 03.06.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

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1.3 The values stated in SI units are to be regarded as the standard.  
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SIGNIFICANCE AND USE
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1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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