EN ISO 19020:2017

Microbiology of the food chain - Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs (ISO 19020:2017)

ISO 19020:2017 specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE in foodstuffs. It consists of two main steps: a) extraction followed by a concentration based on dialysis principle; and b) an immunoenzymatic detection using commercially available detection kits.
ISO 19020:2017 is applicable to the screening of staphylococcal enterotoxins SEA to SEE in products intended for human consumption.
Other staphylococcal enterotoxins such as types SEG, SEH, SEI, SER, SES and SET can also cause illness. Due to the lack of commercially available detection kits, ISO 19020:2017 is applicable only to types SEA to SEE, but may apply to other types of toxins, subject to validation of the method.

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für den immunenzymatischen Nachweis von Staphylokokken-Enterotoxinen in Lebensmitteln (ISO 19020:2017)

Dieses Dokument legt ein Screening-Verfahren für den Nachweis von den Staphylokokken-Enterotoxinen SEA, SEB, SECs, SED und SEE (SEA bis SEE) in Lebensmitteln fest. Die Norm besteht aus zwei Hauptschritten (i) die Extraktion gefolgt von der Konzentration, was auf dem Dialyseprinzip beruht und (ii) ein immunenzymatischer Nachweis unter Anwendung handelsüblicher Nachweiskits.
Diese Internationale Norm ist anwendbar für das Screening von Staphylokokken-Enterotoxinen (SEA-SEE) in Produkten bestimmt für den menschlichen Verzehr.
Andere Staphylokokken-Enterotoxine, wie z. B. die Arten SEG, SEH, SEI, SER, SES und SET, können auch Krankheiten verursachen. Doch aufgrund fehlender handelsüblicher Nachweiskits richtet sich diese Norm nur an die Arten SEA bis SEE.

Microbiologie de la chaîne alimentaire - Méthode horizontale de détection des entérotoxines staphylococciques par test immuno-enzymatique dans les aliments (ISO 19020:2017)

L'ISO 19020:2017 décrit une méthode de recherche pour la détection des entérotoxines staphylococciques SEA, SEB, SEC, SED et SEE dans les aliments. Elle se compose de deux étapes principales: a) une extraction suivie d'une concentration basée sur le principe de la dialyse; et b) une détection immuno-enzymatique au moyen de trousses de détection disponibles dans le commerce.
L'ISO 19020:2017 s'applique à la recherche des entérotoxines staphylococciques SEA à SEE dans les produits destinés à la consommation humaine.
D'autres entérotoxines staphylococciques, telles que les types SEG, SEH, SEI, SER, SES et SET, peuvent également provoquer des intoxications alimentaires. En raison de l'absence de trousses de détection disponibles dans le commerce, l'ISO 19020:2017 ne s'applique qu'aux types SEA à SEE, mais peut s'appliquer à d'autres types de toxines, sous réserve de validation de la méthode.

Mikrobiologija v prehranski verigi - Horizontalna metoda za imunoencimsko ugotavljanje stafilokoknih enterotoksinov v živilih (ISO 19020:2017)

Ta standard opisuje metodo za ugotavljanje stafilokoknih enterotoksinov v mlečnih izdelkih in drugih matricah.

General Information

• Status: Published
• Publication Date: 27-Jun-2017
• Withdrawal Date: 30-Dec-2017
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463 - Microbiology of the food chain
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 28-Jun-2017
• Due Date: 01-Mar-2014
• Completion Date: 28-Jun-2017
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Mandate: M/381 - Mandate for standardisation addressed to CEN in the field of Methods of analysis of foodstuffs concerning Food Hygiene
• Ref Project: SIST EN ISO 19020:2017 - Microbiology of the food chain - Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs (ISO 19020:2017)

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2017
Mikrobiologija v prehranski verigi - Horizontalna metoda za imunoencimsko
ugotavljanje stafilokoknih enterotoksinov v živilih (ISO 19020:2017)
Microbiology of the food chain - Horizontal method for the immunoenzymatic detection of
staphylococcal enterotoxins in foodstuffs (ISO 19020:2017)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für den
immunenzymatischen Nachweis von Staphylokokken-Enterotoxinen in Lebensmitteln
(ISO 19020:2017)
Microbiologie de la chaîne alimentaire - Méthode horizontale de détection des
entérotoxines staphylococciques par test immuno-enzymatique dans les aliments (ISO
19020:2017)
Ta slovenski standard je istoveten z: EN ISO 19020:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 19020
EUROPEAN STANDARD
NORME EUROPÉENNE
June 2017
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of the food chain - Horizontal method for the
immunoenzymatic detection of staphylococcal
enterotoxins in foodstuffs (ISO 19020:2017)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale de détection des entérotoxines Verfahren für den immunenzymatischen Nachweis von
staphylococciques par test immuno-enzymatique dans Staphylokokken-Enterotoxinen in Lebensmitteln (ISO
les aliments (ISO 19020:2017) 19020:2017)
This European Standard was approved by CEN on 14 May 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 19020:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
European foreword
This document (EN ISO 19020:2017) has been prepared CEN/TC 275 “Food analysis - Horizontal
methods” the secretariat of which is held by DIN, in collaboration with Technical Committee ISO/TC 34
“Food products”.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by December 2017, and conflicting national standards
shall be withdrawn at the latest by December 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 19020:2017 has been approved by CEN as EN ISO 19020:2017 without any modification.

INTERNATIONAL ISO
STANDARD 19020
First edition
2017-06
Microbiology of the food chain —
Horizontal method for the
immunoenzymatic detection of
staphylococcal enterotoxins in
foodstuffs
Microbiologie de la chaîne alimentaire — Méthode horizontale de
détection des entérotoxines staphylococciques par test immuno-
enzymatique dans les aliments
Reference number
ISO 19020:2017(E)
©
ISO 2017
ISO 19020:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
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ii © ISO 2017 – All rights reserved

ISO 19020:2017(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus . 2
7 Sampling . 3
8 Procedure . 3
8.1 Preparation of test portion . 3
8.2 Storage of the test sample . 3
8.3 Extraction . 4
8.4 Concentration of the extract (mandatory for milk and dairy products) . 5
8.5 Recovery of the concentrated extract . 5
8.6 Storage and steps before detection . 6
8.7 Detection . 6
8.8 Performance criteria . 6
9 Quality control . 6
10 Expression of results . 7
11 Confirmation . 7
12 Performance characteristics of the method . 7
13 Test report . 9
Annex A (informative) Results of interlaboratory studies: 2013.10
Annex B (informative) Results of interlaboratory studies: 2014.15
Annex C (informative) Note on interferences .21
Bibliography .22
ISO 19020:2017(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: w w w . i s o .org/ iso/ foreword .html.
This document was prepared by the European Committee for Standardization (CEN) Technical
Committee CEN/TC 275, Food Analysis — Horizontal methods, in collaboration with ISO Technical
Committee TC 34, Food products, Subcommittee SC 9, Microbiology, in accordance with the agreement
on technical cooperation between ISO and CEN (Vienna Agreement).
iv © ISO 2017 – All rights reserved

ISO 19020:2017(E)
Introduction
Staphylococcal enterotoxins (SEs) are proteins that can be produced in foods, by certain strains of the
coagulase positive staphylococci (CPS), mainly Staphylococcus aureus. These SEs are heat and acid stable
toxins that cause nausea, vomiting, abdominal pain and diarrhoea when ingested. Due to their stability
SEs might still be present even when coagulase positive staphylococci cannot
...