prEN 370

Wood preservatives - Determination of eradicant efficacy in preventing emergence of Anobium punctatum (De Geer)

This document specifies a method for the determination of the curative action of a wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment to wood.
This method is applicable to any surface-applied treatment that is intended to prevent emergence of adult beetles but not intended to kill larvae in infested timber.
NOTE 1 This method can be used in conjunction with an ageing procedure, for example EN 73.
NOTE 2 Products intended to kill larvae can be tested by the method described in EN 48.

Holzschutzmittel - Bestimmung der auf Schlupfverhinderung beruhenden bekämpfenden Wirksamkeit gegenüber Anobium punctatum (De Geer)

Dieses Dokument legt ein Verfahren zur Bestimmung der bekämpfenden Wirkung eines Holzschutzmittels gegen Befall durch Anobium punctatum (De Geer) fest, wenn das Produkt zur Oberflächenbehandlung auf Holz aufgetragen wird.
Dieses Verfahren ist für jede Oberflächenbehandlung anzuwenden, die dazu bestimmt ist, den Schlupf ausgewachsener Käfer zu verhindern, aber nicht dazu gedacht ist, die Larven in befallenem Holz abzutöten.
ANMERKUNG 1 Dieses Verfahren kann in Verbindung mit einer Alterungsbeanspruchung angewendet werden, z. B. nach EN 73.
ANMERKUNG 2 Produkte zur Abtötung von Larven können durch das in EN 48 beschriebene Verfahren geprüft werden.

Produits de préservation du bois - Détermination de l'efficacité curative contre l'émergence d'Anobium punctatum (De Geer)

Le présent document décrit une méthode de détermination de l’action curative d'un produit de préservation du bois contre l’infestation d'Anobium punctatum (De Geer) lorsque le produit est appliqué au bois par un traitement de surface.
La présente méthode est applicable à tout traitement de surface qui est destiné à empêcher l'émergence d’insectes adultes mais non de tuer les larves dans le bois infesté.
NOTE 1 Cette méthode peut être utilisée conjointement avec une épreuve de vieillissement, par exemple selon l'EN 73.
NOTE 2 Les produits destinés à tuer les larves peuvent être soumis à essai selon la méthode décrite dans l’EN 48.

Zaščitna sredstva za les - Ugotavljanje učinkovitosti preventivnega delovanja proti navadnemu trdoglavcu Anobium punctatum (De Geer)

General Information

• Status: Not Published
• Publication Date: 14-Aug-2024
• ICS: 71.100.50 - Wood-protecting chemicals
• Technical Committee: CEN/TC 38 - Durability of wood and derived materials
• Drafting Committee: CEN/TC 38/WG 24 - Insect testing - (beetles - termites)
• Current Stage: 4060 - Closure of enquiry - Enquiry
• Start Date: 29-Dec-2022
• Due Date: 22-Dec-2022
• Completion Date: 29-Dec-2022
• Directive: 305/2011 - Regulation (eu) No 305/2011 of the European Parliament and of the Council of 9 march 2011 laying down harmonised conditions for the marketing of construction products and repealing council directive 89/106/eec
• Ref Project: oSIST prEN 370:2022 - Wood preservatives - Determination of eradicant efficacy in preventing emergence of Anobium punctatum (De Geer)

Relations

• Revises: EN 370:1993 - Wood preservatives - Determination of eradicant efficacy in preventing emergence of Anobium punctatum (De Geer)
• Effective Date: 19-Jan-2023

Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN 370:2022
01-december-2022

Zaščitna sredstva za les - Ugotavljanje učinkovitosti preventivnega delovanja proti

navadnemu trdoglavcu Anobium punctatum (De Geer)

Wood preservatives - Determination of eradicant efficacy in preventing emergence of

Anobium punctatum (De Geer)

Holzschutzmittel - Bestimmung der auf Schlupfverhinderung beruhenden bekämpfenden

Wirksamkeit gegenüber Anobium punctatum (De Geer)
Produits de préservation du bois - Détermination de l'efficacité curative contre
l'émergence d'Anobium punctatum (De Geer)
Ta slovenski standard je istoveten z: prEN 370
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
oSIST prEN 370:2022 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN 370:2022
---------------------- Page: 2 ----------------------
oSIST prEN 370:2022
DRAFT
EUROPEAN STANDARD
prEN 370
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2022
ICS 71.100.50 Will supersede EN 370:1993
English Version
Wood preservatives - Determination of eradicant efficacy
in preventing emergence of Anobium punctatum (De Geer)

Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der auf

l'efficacité curative contre l'émergence d'Anobium Schlupfverhinderung beruhenden bekämpfenden

punctatum (De Geer) Wirksamkeit gegenüber Anobium punctatum (De
Geer)

This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee

CEN/TC 38.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations

which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other

language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC

Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and

United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are

aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without

notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 370:2022 E

worldwide for CEN national Members.
---------------------- Page: 3 ----------------------
oSIST prEN 370:2022
prEN 370:2022 (E)
Contents Page

European foreword ....................................................................................................................................................... 4

Introduction .................................................................................................................................................................... 5

1 Scope .................................................................................................................................................................... 6

2 Normative references .................................................................................................................................... 6

3 Terms and definitions ................................................................................................................................... 6

4 Principle ............................................................................................................................................................. 6

5 Test materials and apparatus ..................................................................................................................... 6

5.1 Biological material .......................................................................................................................................... 6

5.1.1 Anobium punctatum (De Geer) larvae ..................................................................................................... 6

5.1.2 Provision of larvae .......................................................................................................................................... 7

5.1.3 Choice of larvae ................................................................................................................................................ 7

5.2 Products and reagents ................................................................................................................................... 7

5.3 Apparatus ........................................................................................................................................................... 7

6 Sampling ............................................................................................................................................................. 8

7 Test specimens ................................................................................................................................................. 8

7.1 Species of wood ................................................................................................................................................ 8

7.2 Quality of wood ................................................................................................................................................ 8

7.3 Provision of test specimens ......................................................................................................................... 9

7.4 Dimension of test specimens ...................................................................................................................... 9

7.5 Number of test specimens ............................................................................................................................ 9

8 Procedure........................................................................................................................................................... 9

8.1 Preparation of the test specimens ............................................................................................................ 9

8.1.1 Sealing of the transverse faces ................................................................................................................... 9

8.1.2 Treatment of test specimens .................................................................................................................... 10

8.2 Exposure of the test specimens to the insects ................................................................................... 11

8.2.1 Preparation of sub-specimens ................................................................................................................. 11

8.2.2 Insertion of larvae........................................................................................................................................ 11

8.2.3 Conditioning of infested sub-specimens to induce emergence ................................................... 11

8.3 Examination of sub-specimens ............................................................................................................... 12

9 Validity of the test ........................................................................................................................................ 12

10 Expression of results ................................................................................................................................... 12

11 Test report ...................................................................................................................................................... 12

Annex A (informative) Example of a test report ............................................................................................ 16

Annex B (informative) Culturing Technique for Anobium punctatum .................................................. 18

B.1 Culture wood .................................................................................................................................................. 18

B.1.1 Wood species ................................................................................................................................................. 18

B.1.2 Collection of culture wood ........................................................................................................................ 18

B.1.3 Cutting of culture wood .............................................................................................................................. 18

B.1.4 Drying of culture wood ............................................................................................................................... 18

---------------------- Page: 4 ----------------------
oSIST prEN 370:2022
prEN 370:2022 (E)

B.2 Source of beetles ........................................................................................................................................... 18

B.2.1 Collection of beetles ..................................................................................................................................... 18

B.2.2 Quarantine of beetles .................................................................................................................................. 18

B.3 Infestation of culture wood ....................................................................................................................... 18

B.3.1 Culture vessels ............................................................................................................................................... 18

B.3.2 Preparation of wood .................................................................................................................................... 19

B.3.3 Introduction of beetles ............................................................................................................................... 19

B.4 Culturing conditions .................................................................................................................................... 19

B.4.1 Normal environment ................................................................................................................................... 19

B.4.2 Natural pupation induction ...................................................................................................................... 19

B.4.3 Artificial pupation induction .................................................................................................................... 19

B.5 Collection of beetles ..................................................................................................................................... 19

B.6 General culture hygiene ............................................................................................................................. 19

Bibliography ................................................................................................................................................................. 21

---------------------- Page: 5 ----------------------
oSIST prEN 370:2022
prEN 370:2022 (E)
European foreword

This document (prEN 370:2022) has been prepared by Technical Committee CEN/TC 38 “Durability of

wood and wood-based products”, the secretariat of which is held by AFNOR.
This document is currently submitted to the CEN Enquiry.
This document will supersede EN 370:1993.

Significant technical differences between this document and EN 370:1993 are as follows:

a) Maximum recommended quantity of test solution application was changed from 250 g/m to

300 ml/m (8.1.2.2 Note 2);

b) The count of adults for validity of the test may include those hatching underneath the cover-ups of

the end faces (9).

NOTE Test results obtained according to earlier versions of this document and when the tests had started

before this version of EN 370 was published are considered as valid.
---------------------- Page: 6 ----------------------
oSIST prEN 370:2022
prEN 370:2022 (E)
Introduction

This document describes a laboratory method of test which gives a basis for assessment of the eradicant

efficacy of a wood preservative, in preventing emergence of Anobium punctatum. It determines the lethal

effects of an insecticidal product, deposited by surface application, on beetles attempting to emerge

through treated wood surfaces.

The method has been developed to assess the efficacy of eradicant formulations based on non-

penetrating fluids which act only on emerging adult beetles and not at depth on larvae established in the

wood.

The method simulates conditions which can appear in practice where a length of timber infested with

Anobium punctatum is treated on all the sides from which emergence of beetles is possible.

This laboratory method provides one criterion by which the value of a product can be assessed. In making

this assessment, the methods by which the preservative may be applied should be taken into account. It

is further recommended that results from this test should be supplemented by those from other

appropriate tests, and above all by comparison with practical experience.

When products which are very active at low concentrations are used, it is very important to take suitable

precautions to isolate and separate, as far as possible, operations involving chemical products, other

products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use

of separate rooms, areas within rooms, extraction facilities and conditioning chambers as well as special

training for personnel.
---------------------- Page: 7 ----------------------
oSIST prEN 370:2022
prEN 370:2022 (E)
1 Scope

This document specifies a method for the determination of the curative action of a wood preservative

against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment

to wood.

This method is applicable to any surface-applied treatment that is intended to prevent emergence of adult

beetles but not intended to kill larvae in infested timber.

NOTE 1 This method can be used in conjunction with an ageing procedure, for example EN 73.

NOTE 2 Products intended to kill larvae can be tested by the method described in EN 48.

2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
representative sample

sample having its physical or chemical characteristics identical to the volumetric average characteristics

of the total volume being sampled
3.2
supplier
sponsor of the test
4 Principle

Preservative is applied by brush or pipette onto test specimens of a susceptible timber. After drying the

test specimens are cut into two sub-specimens and larvae of Anobium punctatum are introduced into the

freshly-cut end grain surfaces.

After allowing larvae to establish, the untreated faces are sealed and insects are induced to pupate and

emerge. The numbers of beetles that emerge and the population that remains within the specimens are

compared with those in untreated controls.
5 Test materials and apparatus
5.1 Biological material
5.1.1 Anobium punctatum (De Geer) larvae

The culturing technique, which experience has shown to be suitable, is described in Annex B.

---------------------- Page: 8 ----------------------
oSIST prEN 370:2022
prEN 370:2022 (E)
5.1.2 Provision of larvae

Carefully split or crumble infested small branchwood to extract larvae. Examine them under a binocular

microscope and destroy any that show injury or mite infestation or that do not respond by movement

when touched.

Weigh the larvae and keep those that have a mass between 7 mg and 12 mg, and are in perfect condition.

Keep them, for between 12 h and 60 h, separately from one another in glass receptacles in the culturing

chamber (5.3.1). Re-examine them and reject any which do not show movement in response to

stimulation with a fine brush.
5.1.3 Choice of larvae

Select sets of 12 larvae so that the total mass of each set is between 100 mg and 125 mg.

The numbers of larvae required are shown in Table 1.
Table 1 — Numbers of larvae
Number of test specimens
(100 mm × 50 mm × 30 mm)
Number of
Total number of
formulations to be required
larvae required
tested
Untreated Treated
controls specimens
1 3 3 144
2 3 6 216
3 6 9 360
4 6 12 432

NOTE Additional larvae can be required to replace larvae which do not establish in the test sub-specimens.

5.2 Products and reagents
5.2.1 Water, complying with grade 3 of ISO 3696.

5.2.2 Gelatin, for sealing the relevant surfaces of specimens to be treated with solutions in which an

organic solvent is the continuous phase.

5.2.3 Paraffin wax, for sealing the relevant surfaces of specimens to be treated with solutions in which

water is the continuous phase.

NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.

5.3 Apparatus

5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity

(75 ± 5) %.

5.3.2 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.

CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials. Avoid

excessive exposure of operators to solvents or their vapours.
(75 ± 5) %.

5.3.3 Testing chamber, ventilated, controlled at (21 ± 2) °C and at relative humidity

---------------------- Page: 9 ----------------------
oSIST prEN 370:2022
prEN 370:2022 (E)
5.3.4 Low temperature regime chamber
— Either:

— ventilated and controlled to provide a continuous temperature regime with consecutive cycles

of 12 h at (6 ± 1) °C and 12 h at (13 ± 1) °C; or
— Ventilated and controlled at (6 ± 1) °C and relative humidity (70 ± 5) %.

5.3.5 Drill, provided with bits capable of drilling smooth cylindrical holes of 2 mm diameter in

wood.
5.3.6 Plastic plates of opaque unplasticised PVC, 50 mm × 30 mm × 1 mm.

5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test solvent,

to ensure the safety of the operator.

5.3.8 Pipette, of type specified in ISO 835, Part 1, Class 8: graduated pipette with no waiting time.

Capacity from 0,5 ml to 25 ml with an accuracy of ± 0,01 mi.

5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of

0,01 g.
6 Sampling

The sample of preservative shall be representative of the product to be tested. Samples shall be stored

and handled in accordance with any written recommendations from the supplier.

For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.

7 Test specimens
7.1 Species of wood

The test shall be carried out on Pinus sylvestris (Linnaeus) European redwood, Scots pine.

Additional tests may be done with other species such as beech (Fagus sylvatica) (Linnaeus) but, if so, this

should be stated in the test report.
7.2 Quality of wood
Use only sound sapwood, straight-grained and without knots and bark.

The wood shall have an average growth of between 2 annual growth rings per 10 mm and 8 annual

growth rings per 10 mm (two annual growth rings per 10 mm to six annual rings per 10 mm for beech).

It is recommended to use test specimens of similar growth rate within a single test.

Only sapwood with a low resin content shall be used.

The proportion of summer wood in the annual rings shall not exceed 30 % of the whole.

The wood shall have been ne
...