EN ISO 22579:2021

Infant formula and adult nutritionals - Determination of fructans - High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) after enzymatic treatment (ISO 22579:2020)

This document specifies a method for the determination of inulin-type fructans (including oligofructose, fructooligosaccharides) in infant formula and adult nutritionals (both powder and liquid) containing 0,03 g/100 g to 5,0 g/100 g of fructans in the product as prepared ready for consumption.
The method has been validated in a multi laboratory study[1] with reconstituted standard reference material (SRM), infant/adult nutritional formula at a level of 0,204 g/100 g, adult nutritionals ready-to-feed (RTF) at levels of 1,28 g/100 g and 2,67 g/100 g, infant formula RTF at a level of 0,300 g/100 g, reconstituted follow-up formula at levels of 0,209 g/100 g to 0,275 g/100 g, reconstituted infant formula at levels from 0,030 8 g/100 g to 0,264 g/100 g. During the single laboratory validation study[2], spike-recovery experiments were performed up to 5 g/100 g in reconstituted infant formula powders (milk-based, partially hydrolysed milk-based and soy-based), adult nutritional RTF and reconstituted adult nutritional powders.

Säuglingsnahrung und Nahrungsergänzungsmittel für Erwachsene - Bestimmung von Fructanen - Hochleistungs-Anionenaustausch-Chromatographieverfahren mit gepulster amperometrischer Detektion (HPAEC-PAD) nach enzymatischer Behandlung (ISO 22579:2020)

WARNUNG — Das in diesem Dokument beschriebene Verfahren verwendet ätzende (Natrium¬hydroxid, Essigsäure) und giftige (Natriumazid) Chemikalien. Es sind die Sicherheitsdatenblätter zu beachten und entsprechende zusätzliche Sicherheitsvorkehrungen für die Handhabung und Ent¬sorgung zu treffen.
Dieses Dokument legt ein Verfahren zur Bestimmung von Fructanen des Inulintyps (einschließlich Oligo-fruktose, Fruktooligosaccharide) in Säuglingsnahrung und Nahrungsergänzungsmitteln für Erwachsene (sowohl Pulver als auch Flüssigkeit) fest, die 0,03 g/100 g bis 5,0 g/100 g Fructane in dem verzehrfertigen Enderzeugnis enthalten.
Das Verfahren wurde in einer Multi-Laborstudie [1] mit rekonstituiertem Standardreferenzmaterial (SRM) für Säuglingsnahrung/Nahrungsergänzungsmittel für Erwachsene mit einem Gehalt von 0,204 g/100 g, Nahrungsergänzungsmittel für Erwachsene als Fertignahrung (RTF, en: ready-to-feed) mit Gehalten von 1,28 g/100 g und 2,67 g/100 g, Säuglingsnahrung als Fertignahrung mit einem Gehalt von 0,300 g/100 g, rekonstituierte Folgenahrung im Bereich von 0,209 g/100 g bis 0,275 g/100 g, rekonstituierte Säuglingsnahrung im Bereich von 0,030 8 g/100 g bis 0,264 g/100 g validiert. Während der Validierungs-studie im Einzellabor [2] wurden Versuche zur Wiederfindungsrate bei Aufstockung von bis zu 5 g/100 g in rekonstituierten Säuglingsnahrungspulvern (auf Milchbasis, auf teilweise hydrolysierter Milchbasis und Sojabasis), Nahrungsergänzungsmitteln für Erwachsene als Fertignahrung und rekonstituierten pulver-förmigen Nahrungsergänzungsmitteln für Erwachsene durchgeführt.

Préparations pour nourrissons et produits nutritionnels pour adultes - Dosage des fructanes - Chromatographie échangeuse d'anions haute performance couplée à la détection par ampérométrie pulsée (CEAHP-DAP) après traitement enzymatique (ISO 22579:2020)

Le présent document spécifie une méthode de dosage des fructanes de type inuline (tels que l'oligofructose, les fructo-oligosaccharides) dans les formules pour nourrissons et les produits nutritionnels pour adultes (en poudre ou liquides) contenant 0,03 g/100 g à 5,0 g/100 g de fructanes dans le produit préparé sous une forme prête à la consommation.
La méthode a été validée lors d'une étude interlaboratoires[1] avec un matériau de référence standard (SRM) reconstitué de type préparation nutritionnelle pour nourrissons/adultes à une concentration de 0,204 g/100 g, produits nutritionnels prêts à l'emploi (RTF) pour adultes à des concentrations de 1,28 g/100 g et 2,67 g/100 g, préparation RTF pour nourrissons à une concentration de 0,300 g/100 g, préparation de suite reconstituée à des concentrations de 0,209 g/100 g à 0,275 g/100 g, préparation reconstituée pour nourrissons à des concentrations de 8 g/100 g à 0,264 g/100 g. Pendant l'étude de validation intralaboratoire[2], des essais de recouvrement ont été menés jusqu'à 5 g/100 g dans les préparations en poudre reconstituées pour nourrissons (à base de lait, de lait partiellement hydrolysé et de soja), le produit nutritionnel RTF et les produits nutritionnels reconstitués pour adultes.

Hrana za dojenčke in prehranska dopolnila za odrasle - Določevanje fruktanov - Anionska izmenjevalna kromatografija z visoko ločljivostjo s pulzno amperometrično detekcijo (HPAEC-PAD) po encimski obdelavi (ISO 22579:2020)

General Information

• Status: Published
• Publication Date: 16-Feb-2021
• Withdrawal Date: 30-Aug-2021
• ICS: 67.050 - General methods of tests and analysis for food products
• Technical Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Drafting Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 17-Feb-2021
• Completion Date: 17-Feb-2021
• Ref Project: SIST EN ISO 22579:2021 - Infant formula and adult nutritionals - Determination of fructans - High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) after enzymatic treatment (ISO 22579:2020)

Standards Content (Sample)

SLOVENSKI STANDARD
01-april-2021
Hrana za dojenčke in prehranska dopolnila za odrasle - Določevanje fruktanov -
Anionska izmenjevalna kromatografija z visoko ločljivostjo s pulzno
amperometrično detekcijo (HPAEC-PAD) po encimski obdelavi (ISO 22579:2020)
Infant formula and adult nutritionals - Determination of fructans - High performance anion
exchange chromatography with pulsed amperometric detection (HPAEC-PAD) after
enzymatic treatment (ISO 22579:2020)
Säuglingsnahrung und Nahrungsergänzungsmittel für Erwachsene - Bestimmung von
Fructanen - Hochleistungs-Anionenaustausch-Chromatographieverfahren mit gepulster
amperometrischer Detektion (HPAEC-PAD) nach enzymatischer Behandlung (ISO
22579:2020)
Formules infantiles et produits nutritionnels pour adultes - Détermination de la teneur en
fructanes - Chromatographie déchange danions à haute performance couplée à la
détection par ampérométrie pulsée « CEAHP-DAP » après traitement enzymatique (ISO
22579:2020)
Ta slovenski standard je istoveten z: EN ISO 22579:2021
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 22579
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2021
EUROPÄISCHE NORM
ICS 67.050
English Version
Infant formula and adult nutritionals - Determination of
fructans - High performance anion exchange
chromatography with pulsed amperometric detection
(HPAEC-PAD) after enzymatic treatment (ISO
22579:2020)
Préparations pour nourrissons et produits Säuglingsnahrung und Nahrungsergänzungsmittel für
nutritionnels pour adultes - Dosage des fructanes - Erwachsene - Bestimmung von Fructanen -
Chromatographie échangeuse d'anions haute Hochleistungs-Anionenaustausch-
performance couplée à la détection par ampérométrie Chromatographieverfahren mit gepulster
pulsée (CEAHP-DAP) après traitement enzymatique amperometrischer Detektion (HPAEC-PAD) nach
(ISO 22579:2020) enzymatischer Behandlung (ISO 22579:2020)
This European Standard was approved by CEN on 22 September 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22579:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 22579:2021) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2021, and conflicting national standards shall
be withdrawn at the latest by August 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 22579:2020 has been approved by CEN as EN ISO 22579:2021 without any modification.

INTERNATIONAL ISO
STANDARD 22579
IDF 241
First edition
2020-09
Infant formula and adult
nutritionals — Determination of
fructans — High performance anion
exchange chromatography with pulsed
amperometric detection (HPAEC-PAD)
after enzymatic treatment
Préparations pour nourrissons et produits nutritionnels pour
adultes — Dosage des fructanes — Chromatographie échangeuse
d'anions haute performance couplée à la détection par ampérométrie
pulsée (CEAHP-DAP) après traitement enzymatique
Reference numbers
ISO 22579:2020(E)
IDF 241:2020(E)
©
ISO and IDF 2020
ISO 22579:2020(E)
IDF 241:2020(E)
© ISO and IDF 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: [phone] Phone: [phone]
Fax: [phone]
Email: [e-mail] Email: [e-mail]
Website: www.iso.org Website: www.fil-idf.org
Published in Switzerland
ii © ISO and IDF 2020 – All rights reserved

ISO 22579:2020(E)
IDF 241:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Chemicals and reagents . 2
5.1 List of chemicals and reagents . 2
5.2 Preparation of reagents . 3
5.3 Preparation of mobile phases using column A (6.13.1) or equivalent. 5
5.4 Preparation of mobile phases using columns B (6.13.2) or equivalent . 5
5.5 Preparation of standard solutions . 6
6 Apparatus . 6
7 Procedure. 8
7.1 Sample preparation . 8
7.1.1 Powdered or concentrated products on a ready-to feed (RTF) basis and
powder products inhomogeneous at the subgram level . 8
7.1.2 Reconstituted products as prepared in 7.1.1 or products sold as RTF . 8
7.1.3 Homogeneous powdered products without prior reconstitution . 8
7.1.4 Dilution. 8
7.1.5 Hydrolysis of sucrose and α-glucans . 8
7.1.6 Carrez clarification (optional, use in case of difficulties passing sample
through SPE) . 8
7.1.7 Removal of monosaccharides . 9
7.1.8 Hydrolysis of fructans . . . 9
7.2 Chromatographic conditions using column A (6.13.1) . 9
7.3 Chromatographic conditions using columns B (6.13.2) .10
7.4 System suitability test .11
7.5 Calibration .11
8 Calculation .11
9 Reporting .13
10 Precision data .13
10.1 General .13
10.2 Repeatability .13
10.3 Reproducibility .13
11 Test report .14
Annex A (informative) Example chromatograms and calibration curves .15
Annex B (informative) Precision data .18
Annex C (informative) Check test enzyme mixture of sucrase, β-amylase, pullulanase and
maltase.19
Bibliography .22
ISO 22579:2020(E)
IDF 241:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national
standards bodies (ISO memb
...