EN ISO 6579:2002
(Main)Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp (ISO 6579:2002)
Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp (ISO 6579:2002)
ISO 6579:2002 specifies a horizontal method for the detection of Salmonella, including Salmonella Typhi and Salmonella Paratyphi.
It is applicable to products intended for human consumption and the feeding of animals, and to environmental samples in the area of food production and food handling.
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zum Nachweis von Salmonellen spp (ISO 6579:2002)
Diese Internationale Norm legt ein horizontales Verfahren zum Nachweis von Salmonellen fest, einschließlich Salmonella Typhi und Salmonella Paratyphi.
In Abhängigkeit von den in der Einleitung erörterten Einschränkungen gilt diese Internationale Norm für
¾ Produkte, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind;
¾ Umgebungsproben im Bereich der Herstellung von Lebensmitteln und beim Umgang mit Lebensmitteln.
WARNUNG - Es ist möglich, dass nach diesem Untersuchungsverfahren nicht alle Salmonella Typhi und Salmonella Paratyphi wachsen.
Microbiologie des aliments - Méthode horizontale pour la recherche des Salmonella spp (ISO 6579:2002)
La présente Norme internationale spécifie une méthode horizontale de recherche des Salmonella, incluant Salmonella Typhi et Salmonella Paratyphi.
La présente Norme internationale est applicable aux produits destinés à la consommation humaine ou à l'alimentation animale.
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje prisotnosti vrst Salmonella (ISO 6579:2002)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-april-2003
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje prisotnosti vrst
Salmonella (ISO 6579:2002)
Microbiology of food and animal feeding stuffs - Horizontal method for the detection of
Salmonella spp (ISO 6579:2002)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zum
Nachweis von Salmonellen spp (ISO 6579:2002)
Microbiologie des aliments - Méthode horizontale pour la recherche des Salmonella spp
(ISO 6579:2002)
Ta slovenski standard je istoveten z: EN ISO 6579:2002
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 6579
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2002
ICS 07.100.30 Supersedes EN ISO 12824:1997
English version
Microbiology of food and animal feeding stuffs - Horizontal
method for the detection of Salmonella spp (ISO 6579:2002)
Microbiologie des aliments - Méthode horizontale pour la Mikrobiologie von Lebensmitteln und Futtermitteln -
recherche des Salmonella spp (ISO 6579:2002) Horizontales Verfahren zum Nachweis von Salmonellen
spp (ISO 6579:2002)
This European Standard was approved by CEN on 30 May 2002.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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EUROPÄISCHES KOMITEE FÜR NORMUNG
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© 2002 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6579:2002 E
worldwide for CEN national Members.
CORRECTED 2002-09-18
Foreword
This document (ISO 6579:2002) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 275 "Food
analysis - Horizontal methods", the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by January 2003, and conflicting national
standards shall be withdrawn at the latest by January 2003.
This document supersedes EN 12824:1997.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Malta, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the
United Kingdom.
Endorsement notice
The text of ISO 6579:2002 has been approved by CEN as EN ISO 6579:2002 without any
modifications.
NOTE Normative references to International Standards are listed in Annex ZA (normative).
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions
of any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications,
indicated by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 6887-1 1999 Microbiology of food and animal feeding EN ISO 6887-1 1999
stuffs — Preparation of test samples, initial
suspension and decimal dilutions for
microbiological examination — Part 1:
General rules for the preparation of the
initial suspension and decimal dilutions
ISO 8261 2001 Milk and milk products - General guidance EN ISO 8261 2001
for the preparation of test samples, initial
suspensions and decimal dilutions for
microbiological examination
INTERNATIONAL ISO
STANDARD 6579
Fourth edition
2002-07-15
Microbiology of food and animal feeding
stuffs — Horizontal method for the
detection of Salmonella spp.
Microbiologie des aliments — Méthode horizontale pour la recherche des
Salmonella spp.
Reference number
ISO 6579:2002(E)
©
ISO 2002
ISO 6579:2002(E)
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ii © ISO 2002 – All rights reserved
ISO 6579:2002(E)
Contents Page
Foreword.iv
Introduction.v
1 Scope .1
2 Normative references.1
3 Terms and definitions .1
4 Principle.2
4.1 General.2
4.2 Pre-enrichment in non-selective liquid medium .2
4.3 Enrichment in selective liquid media .2
4.4 Plating out and identification .2
4.5 Confirmation of identity .2
5 Culture media, reagents and sera.3
5.1 General.3
5.2 Culture media and reagents .3
5.3 Sera .4
6 Apparatus and glassware .4
7 Sampling.5
8 Preparation of test sample.5
9 Procedure (see diagram in annex A).5
9.1 Test portion and initial suspension .5
9.2 Non-selective pre-enrichment .6
9.3 Selective enrichment.6
9.4 Plating out and identification .6
9.5 Confirmation .6
10 Expression of results .10
11 Test report .10
12 Quality assurance.11
Annex A (normative) Diagram of procedure .12
Annex B (normative) Composition and preparation of culture media and reagents.14
Annex C (informative) Results of interlaboratory trial .24
Bibliography.27
ISO 6579:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
The main task of technical committees is to prepare International Standards. Draft International Standards adopted
by the technical committees are circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 6579 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology.
This fourth edition cancels and replaces the third edition (ISO 6579:1993), which has been technically revised.
Annexes A and B form a normative part of this Interntional Standard. Annex C is for information only.
iv © ISO 2002 – All rights reserved
ISO 6579:2002(E)
Introduction
Because of the large variety of food and feed products, this horizontal method may not be appropriate in every
detail for certain products. In this case, different methods, which are specific to these products, may be used if
absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this
horizontal method as far as possible.
When this International Standard is next reviewed, account will be taken of all information then available regarding
the extent to which this horizontal method has been followed and the reasons for deviations from this method in the
case of particular products.
The harmonization of test methods cannot be immediate, and for certain groups of products International
Standards and/or national standards may
...
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