EN ISO 19458:2006
(Main)Water quality - Sampling for microbiological analysis (ISO 19458:2006)
Water quality - Sampling for microbiological analysis (ISO 19458:2006)
ISO 19458:2006 provides guidance on planning water sampling regimes, on sampling procedures for microbiological analysis and on transport, handling and storage of samples until analysis begins. ISO 19458:2006 focuses on sampling for microbiological investigations.
Wasserbeschaffenheit - Probenahme für mikrobiologische Untersuchungen (ISO 19458:2006)
Diese Internationale Norm gibt eine Anleitung zur Planung eines Probenahmeprogramms, zur Vorgehensweise bei Probenahmen für mikrobiologische Analysen und für den Transport, die Handhabung und die Lagerung von Proben bis zum Beginn der Untersuchung. Im Mittelpunkt steht die Probenahme für mikrobiologische Untersuchungen.
Allgemeine Hinweise bezüglich der Probenahme aus bestimmten Wasserkörpern sind in den entsprechenden Teilen der ISO 5667 enthalten.
Qualité de l'eau - Échantillonnage pour analyse microbiologique (ISO 19458:2006)
L'ISO 19458:2006 fournit des conseils sur les régimes de planification du prélèvement d'eau, sur les modes opératoires de prélèvement en vue de l'analyse microbiologique et le transport, et sur la manipulation et la conservation des échantillons avant le début de l'analyse. L'ISO 19458:2006 porte sur les prélèvements pour recherches microbiologiques.
Kakovost vode - Vzorčenje za mikrobiološke analize (ISO 19458:2006)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2007
.DNRYRVWYRGH9]RUþHQMH]DPLNURELRORãNHDQDOL]H ,62
Water quality - Sampling for microbiological analysis (ISO 19458:2006)
Wasserbeschaffenheit - Probenahme für mikrobiologische Untersuchungen (ISO
19458:2006)
Qualité de l'eau - Échantillonnage pour analyse microbiologique (ISO 19458:2006)
Ta slovenski standard je istoveten z: EN ISO 19458:2006
ICS:
13.060.45 Preiskava vode na splošno Examination of water in
general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 19458
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2006
ICS 13.060.45
English Version
Water quality - Sampling for microbiological analysis (ISO
19458:2006)
Qualité de l'eau - Échantillonnage pour analyse Wasserbeschaffenheit - Probenahme für mikrobiologische
microbiologique (ISO 19458:2006) Untersuchungen (ISO 19458:2006)
This European Standard was approved by CEN on 1 July 2006.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
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Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2006 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 19458:2006: E
worldwide for CEN national Members.
Foreword
This document (EN ISO 19458:2006) has been prepared by Technical Committee ISO/TC 147
"Water quality" in collaboration with Technical Committee CEN/TC 230 "Water analysis", the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by February 2007, and conflicting national
standards shall be withdrawn at the latest by February 2007.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 19458:2006 has been approved by CEN as EN ISO 19458:2006 without any
modifications.
INTERNATIONAL ISO
STANDARD 19458
First edition
2006-08-01
Water quality — Sampling
for microbiological analysis
Qualité de l'eau — Échantillonnage pour analyse microbiologique
Reference number
ISO 19458:2006(E)
©
ISO 2006
ISO 19458:2006(E)
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ii © ISO 2006 – All rights reserved
ISO 19458:2006(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Sampling point . 1
4 Sampling technique. 2
5 Transport and storage. 10
Annex A (informative) A priori determination of the number of samples to analyse to determine
the mean concentration of microbes in water with a given confidence, for quantitative
determination derived by cultivation of microorganisms . 13
Annex B (informative) Recommended (R) and acceptable (A) values for maximum sample storage
times including transport time and temperatures unless otherwise specified in specific
standards. 16
Bibliography . 17
ISO 19458:2006(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 19458 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 4,
Microbiological methods.
iv © ISO 2006 – All rights reserved
ISO 19458:2006(E)
Introduction
Appropriate sampling is essential to provide representative samples to the laboratory in charge of testing.
Sampling features depend on the objective of sampling, but also on the nature of the sample. Microorganisms
are living organisms. In addition, when they are introduced into water, they do not form a perfect solution, but
a suspension with an inherent degree of variability.
Sampling objectives may serve different purposes, which are described in the ISO 5667 series of standards
(ISO 5667-1, ISO 5667-2 and ISO 5667-3):
a) determination of the compliance of a water with a regulatory quality specification;
b) characterization of any contamination, its level (mean) and its variations:
1) what is its random variation?
2) is there a trend?
3) are there cycles?
c) identification of the sources of pollution.
Regarding the number or frequency of samples, it will vary according to the aim of the sampling.
The minimum number of samples will be low if the mean concentration differs greatly from the specification
(much lower or much higher), and the minimum number of samples will be higher if the mean concentration
and the specification are close to one another. Similarly, in case b), when looking for a trend: the less obvious
the trend, the higher the frequency of sampling (see also Annex A).
INTERNATIONAL STANDARD ISO 19458:2006(E)
Water quality — Sampling for microbiological analysis
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This standard does not purport to address all of the safety problems, if any, associated with
its use. It is the responsibility of the user to establish appropriate safety and health practices and to
ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this standard be carried
out by suitably trained staff.
1 Scope
This International Standard provides guidance on planning water sampling regimes, on sampling procedures
for microbiological analysis and on transport, handling and storage of samples until analysis begins. It focuses
on sampling for microbiological investigations.
General information in respect to the sampling from distinct water bodies is given in the respective parts of
ISO 5667.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 5667-1, Water quality — Sampling — Part 1: Guidance on the design of sampling programmes and
sampling techniques
ISO 5667-2, Water quality — Sampling — Part 2: Guidance on sampling techniques
ISO 5667-3, Water quality — Sampling — Part 3: Guidance on the preservation and handling of water
samples
3 Sampling point
The sampling site shall provide representative characteristics and account for any vertical, horizontal and
temporal variations and shall be identified precisely following the general recommendations of ISO 5667-1 and
ISO 5667-2, taking into account additional aspects specific to microbiology.
Sampling points where conditions are unstable should be avoided, and the heterogeneity of the hydraulic
system shall be taken into consideration. In studies on the efficacy of disinfection, the sampling point shall be
chosen to ensure that the reaction is complete.
EXAMPLE Examples of how the heterogeneity of the system may influence the results are given below.
⎯ It is not equivalent to take a subsurface or a surface sample, or a subsurface sample “contaminated” during recovery
through the surface film. In some instances (e.g. lakes, swimming pools), the concentration in the surface film can be
1 000 times higher than in the subsurface.
ISO 19458:2006(E)
⎯ All the points of a network are not equivalent, as there may be dead ends and sections where the flow is reduced,
particularly if the network is fed from two sources.
⎯ The quality at the outlet of a well-mixed tank is generally the same as in the body of water, but can be quite different
from the inlet.
4 Sampling technique
4.1 Personnel
Formal training, training records and determination of competence shall be described for all those who sample,
and this information shall be properly documented.
4.2 Sample containers
4.2.1 General
For routine samples (for example, sampling at taps, recreational waters, swimming pool waters), use clean,
sterile bottles. The volume of the bottles should be adequate for analysis of all requested parameters.
For sampling by immersion in clean waters, use bottles that are sterile both inside and out and protected, for
example, by kraft paper (to keep dry after autoclaving), aluminium foil or by plastic outer bags.
If not autoclavable, sterilization with gamma rays or by ethylene oxide may be used. The bag can then be
opened just before sampling and can also serve as a glove to hold the bottle to provide maximum asepsis
before being placed on a pole or other sterilizable sampling apparatus.
Alternatively, the outside of sample bottles may be disinfected immediately prior to immersion by a suitable
disinfectant such as isopropanol (4.3.1.1) and allowed to dry before use. This is not suitable for analysis of
spore-forming bacteria.
In most cases, 500 ml bottles are sufficient, as less than five categories of microorganisms are measured,
each involving inoculation of a maximum of 100 ml.
In some cases, larger volumes are necessary, e.g.:
⎯ for bottled water analysis (250 ml per parameter);
⎯ for Legionella spp. or Salmonella spp. (up to 1 l);
⎯ for viruses, Giardia cysts, Cryptosporidium oocysts, amoebae in c
...
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