CEN ISO/TS 16634-2:2009

Food products - Determination of the total nitrogen content by combustion according to the Dumas principle and calculation of the crude protein content - Part 2: Cereals, pulses and milled cereal products (ISO/TS 16634-2:2009)

ISO/TS 16634-2:2009 specifies a method for the determination of the total nitrogen content and the calculation of the crude protein content of cereals, pulses and milled cereal products.
This method, like the Kjeldahl method, does not distinguish between protein nitrogen and non-protein nitrogen. For the calculation of the protein content, various conversion factors, given in the document, are used.

Lebensmittelerzeugnisse - Bestimmung des Gehaltes an Gesamtstickstoff mit dem Verbrennungsverfahren nach Dumas und Berechnung des Gehaltes an Rohprotein - Teil 2: Getreide, Hülsenfrüchte und gemahlene Getreideerzeugnisse (ISO/TS 16634-2:2009)

Dieser Teil von ISO 16634 legt ein Verfahren zur Bestimmung des Gesamtstickstoffgehaltes und zur Berech-nung des Rohproteingehaltes von Getreide, Hülsenfrüchten und gemahlenen Getreideerzeugnissen fest.
Dieses Verfahren unterscheidet, wie auch das Kjeldahl-Verfahren (siehe Literaturhinweise [1] und [6]) nicht zwischen Protein Stickstoff und Nicht Protein Stickstoff. Zur Berechnung des Proteingehaltes werden ver-schiedene Umrechnungsfaktoren verwendet (siehe Anhang D).

Produits alimentaires - Détermination de la teneur en azote total par combustion selon le principe Dumas et calcul de la teneur en protéines brutes - Partie 2: Céréales, légumineuses et produits céréaliers de mouture (ISO/TS 16634-2:2009)

L'ISO 16634-2 spécifie une méthode pour la détermination de la teneur en azote total et le calcul de la teneur en protéines brutes des céréales, légumineuses et produits céréaliers de mouture.
Cette méthode, comme la méthode Kjeldahl, ne distingue pas l'azote protéique de l'azote non protéique. Divers facteurs de conversion sont utilisés pour le calcul de la teneur en protéines.

Živila - Določevanje skupnega dušika s sežigom po Dumasu in izračunom deleža surovih beljakovin - 2. del: Žito, stročnice in žitni mlevski proizvodi (ISO/TS 16634-2:2009)

Ta del ISO 16634 opredeljuje metodo določevanja skupnega dušika in izračun deleža surovih beljakovin pri žitih, stročnicah in žitnih mlevskih proizvodov. Ta metoda, tako kot Kjeldahlova metoda (glej Referenci [1] in [6]), ne razlikuje med dušikom beljakovin in dušikom ne-beljakovin. Za izračun beljakovin se uporabljajo različni pretvorbeni faktorji (glej Prilogo D).

General Information

• Status: Withdrawn
• Publication Date: 30-Sep-2009
• Withdrawal Date: 14-Jun-2016
• ICS: 67.050 - General methods of tests and analysis for food products
• ICS: 67.060 - Cereals, pulses and derived products
• Technical Committee: CEN/TC 338 - Cereal and cereal products
• Drafting Committee: CEN/TC 338 - Cereal and cereal products
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Completion Date: 15-Jun-2016
• Ref Project: SIST-TS CEN ISO/TS 16634-2:2010 - Food products - Determination of the total nitrogen content by combustion according to the Dumas principle and calculation of the crude protein content - Part 2: Cereals, pulses and milled cereal products (ISO/TS 16634-2:2009)

Relations

• Replaced By: EN ISO 16634-2:2016 - Food products - Determination of the total nitrogen content by combustion according to the Dumas principle and calculation of the crude protein content - Part 2: Cereals, pulses and milled cereal products (ISO 16634-2:2016)
• Effective Date: 06-Aug-2014

Standards Content (Sample)

SLOVENSKI STANDARD
01-januar-2010
äLYLOD'RORþHYDQMHVNXSQHJDGXãLNDVVHåLJRPSR'XPDVXLQL]UDþXQRPGHOHåD
VXURYLKEHOMDNRYLQGHOäLWRVWURþQLFHLQåLWQLPOHYVNLSURL]YRGL,6276

Food products - Determination of the total nitrogen content by combustion according to
the Dumas principle and calculation of the crude protein content - Part 2: Cereals, pulses
and milled cereal products (ISO/TS 16634-2:2009)
Lebensmittelerzeugnisse - Bestimmung des Gehaltes an Gesamtstickstoff mit dem
Verbrennungsverfahren nach Dumas und Berechnung des Gehaltes an Rohprotein - Teil
2: Getreide, Hülsenfrüchte und gemahlene Getreideerzeugnisse (ISO/TS 16634-2:2009)
Produits alimentaires - Détermination de la teneur en azote total par combustion selon le
principe Dumas et calcul de la teneur en protéines brutes - Partie 2: Céréales,
légumineuses et produits céréaliers de mouture (ISO/TS 16634-2:2009)
Ta slovenski standard je istoveten z: CEN ISO/TS 16634-2:2009
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.060 äLWDVWURþQLFHLQSURL]YRGLL] Cereals, pulses and derived
QMLK products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

TECHNICAL SPECIFICATION
CEN ISO/TS 16634-2
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
October 2009
ICS 67.050; 67.060
English Version
Food products - Determination of the total nitrogen content by
combustion according to the Dumas principle and calculation of
the crude protein content - Part 2: Cereals, pulses and milled
cereal products (ISO/TS 16634-2:2009)
Produits alimentaires - Détermination de la teneur en azote Lebensmittelerzeugnisse - Bestimmung des Gehaltes an
total par combustion selon le principe Dumas et calcul de la
Gesamtstickstoff mit dem Verbrennungsverfahren nach
teneur en protéines brutes - Partie 2: Céréales, Dumas und Berechnung des Gehaltes an Rohprotein - Teil
légumineuses et produits céréaliers de mouture (ISO/TS 2: Getreide, Hülsenfrüchte und gemahlene
16634-2:2009) Getreideerzeugnisse (ISO/TS 16634-2:2009)
This Technical Specification (CEN/TS) was approved by CEN on 24 August 2009 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN ISO/TS 16634-2:2009: E
worldwide for CEN national Members.

Contents Page
Foreword .3
Foreword
This document (CEN ISO/TS 16634-2:2009) has been prepared by Technical Committee CEN/TC 338
“Cereal and cereal products", the secretariat of which is held by AFNOR, in collaboration with Technical
Committee ISO/TC 34 "Food products".
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
TECHNICAL ISO/TS
SPECIFICATION 16634-2
First edition
2009-10-01
Food products — Determination of the
total nitrogen content by combustion
according to the Dumas principle and
calculation of the crude protein
content —
Part 2:
Cereals, pulses and milled cereal
products
Produits alimentaires — Détermination de la teneur en azote total par
combustion selon le principe Dumas et calcul de la teneur en protéines
brutes —
Partie 2: Céréales, légumineuses et produits céréaliers de mouture

Reference number
ISO/TS 16634-2:2009(E)
©
ISO 2009
ISO/TS 16634-2:2009(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat
accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In
the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.

©  ISO 2009
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.org
Published in Switzerland
ii © ISO 2009 – All rights reserved

ISO/TS 16634-2:2009(E)
Contents Page
Foreword .iv
Introduction.v
1 Scope.1
2 Normative references.1
3 Terms and definitions .1
4 Principle .2
5 Reagents .2
6 Apparatus.3
7 Sampling .3
8 Preparation of test sample .3
9 Procedure.4
9.1 General .4
9.2 Test portion.4
9.3 Control of oxygen supply .4
9.4 Calibration.5
9.5 Determination .5
9.6 Detection and integration .6
10 Calculation and expression of results .6
10.1 Calculation .6
10.2 Expression of results.6
11 Precision .7
11.1 Interlaboratory tests.7
11.2 Repeatability .7
11.3 Reproducibility .7
11.4 Critical difference .7
11.5 Uncertainty.8
12 Test report.8
Annex A (informative) Flowchart for a basic Dumas apparatus .9
Annex B (informative) Schematic diagrams of suitable types of Dumas apparatus .10
Annex C (informative) Equipment calibration.13
Annex D (informative) Examples of factors for converting nitrogen content to protein content.15
Annex E (informative) Results of interlaboratory tests.16
Bibliography.23

ISO/TS 16634-2:2009(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
In other circumstances, particularly when there is an urgent market requirement for such documents, a
technical committee may decide to publish other types of document:
⎯ an ISO Publicly Available Specification (ISO/PAS) represents an agreement between technical experts in
an ISO working group and is accepted for publication if it is approved by more than 50 % of the members
of the parent committee casting a vote;
⎯ an ISO Technical Specification (ISO/TS) represents an agreement between the members of a technical
committee and is accepted for publication if it is approved by 2/3 of the members of the committee casting
a vote.
An ISO/PAS or ISO/TS is reviewed after three years in order to decide whether it will be confirmed for a
further three years, revised to become an International Standard, or withdrawn. If the ISO/PAS or ISO/TS is
confirmed, it is reviewed again after a further three years, at which time it must either be transformed into an
International Standard or be withdrawn.
Attention is drawn to the possibilit
...