prEN 46-1

Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (Laboratory method)

This document specifies a method for the determination of the preventive action of a wood preservative against recently hatched larvae of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood.
This method is applicable to:
-   water-insoluble chemicals which are being studied as active insecticides;
-   organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
-   organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates;
-   water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.

Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber frisch geschlüpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 1: Anwendung durch Oberflächenverfahren (Laboratoriumsverfahren)

Dieses Dokument legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung eines Holzschutzmittels gegenüber frisch geschlüpften Larven (Eilarven) von Hylotrupes bajulus (Linnaeus) fest, wobei das Holzschutzmittel zur Oberflächenbehandlung des Holzes verwendet wird.
Das Verfahren ist anwendbar für:
   wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
   organische Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
   in Wasser dispergierbare organische Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
   wasserlösliche Stoffe, z. B. Salze.
Das Verfahren ist anwendbar unabhängig davon, ob die Prüfkörper einer angemessenen Alterungsbeanspruchung ausgesetzt wurden oder nicht.

Produits de préservation du bois - Détermination de l'action préventive contre les larves récemment écloses d'Hylotrupes bajulus (Linnaeus) - Partie 1 : Application par traitement de surface (Méthode de laboratoire)

Biocidni proizvodi za zaščito lesa - Ugotavljanje preventivnega delovanja proti hišnemu kozličku Hylotrupes bajulus (Linnaeus) - 1. del: Ugotavljanje učinkovitosti proti ličinkam (laboratorijska metoda)

General Information

• Status: Not Published
• Publication Date: 30-Mar-2027
• ICS: 71.100.50 - Wood-protecting chemicals
• Technical Committee: CEN/TC 38 - Durability of wood and derived materials
• Drafting Committee: CEN/TC 38/WG 24 - Insect testing - (beetles - termites)
• Current Stage: 4020 - Submission to enquiry - Enquiry
• Start Date: 09-Oct-2025
• Due Date: 12-Nov-2025
• Completion Date: 09-Oct-2025
• Ref Project: oSIST prEN 46-1:2025 - Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (Laboratory method)

Relations

• Revises: EN 46-1:2016 - Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (laboratory method)
• Effective Date: 12-Apr-2023

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2025
Biocidni proizvodi za zaščito lesa - Ugotavljanje preventivnega delovanja proti
hišnemu kozličku Hylotrupes bajulus (Linnaeus) - 1. del: Ugotavljanje
učinkovitosti proti ličinkam (laboratorijska metoda)
Wood preservatives - Determination of the preventive action against recently hatched
larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment
(Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber frisch
geschlüpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 1: Anwendung durch
Oberflächenverfahren (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'action préventive contre les larves
récemment écloses d'Hylotrupes bajulus (Linnaeus) - Partie 1 : Application par
traitement de surface (Méthode de laboratoire)
Ta slovenski standard je istoveten z: prEN 46-1
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
EUROPEAN STANDARD
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2025
ICS 71.100.50 Will supersede EN 46-1:2016
English Version
Wood preservatives - Determination of the preventive
action against recently hatched larvae of Hylotrupes
bajulus (Linnaeus) - Part 1: Application by surface
treatment (Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden
l'action préventive contre les larves récemment écloses Wirkung gegenüber frisch geschlüpften Larven von
d'Hylotrupes bajulus (Linnaeus) - Partie 1 : Application Hylotrupes bajulus (Linnaeus) - Teil 1: Anwendung
par traitement de surface (Méthode de laboratoire) durch Oberflächenverfahren
(Laboratoriumsverfahren)
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 38.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

E U R O P Ä I SC H E S KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 46-1:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 6
5 Test materials . 7
5.1 Biological material . 7
5.2 Products and reagents . 7
5.3 Apparatus . 7
6 Sampling . 8
7 Test specimens . 8
7.1 Species of wood . 8
7.2 Wood quality . 9
7.3 Provision of test specimens . 9
7.4 Dimensions of test specimens . 9
7.5 Number of test specimens . 9
8 Procedure. 10
8.1 Preparation of the test specimens . 10
8.1.1 Conditioning of the test specimens prior to sealing . 10
8.1.2 Sealing of the transverse faces . 10
8.1.3 Treatment of the test specimens . 10
8.1.4 Drying and conditioning of the test specimens after treatment . 12
8.2 Exposure of the test specimens to the insects . 12
8.3 Conditions and duration of the test . 13
8.4 Examination of the test specimens . 13
8.4.1 Examination . 13
8.4.2 Validity of the test . 14
9 Expression of results . 14
9.1 Evaluation of attack . 14
9.2 Toxic values . 14
10 Test report . 15
Annex A (informative) Example of a test report . 17
Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) . 19
B.1 General . 19
B.2 Obtaining parent beetles . 19
B.3 Mating . 19
B.4 Egg-Iaying . 19
B.5 Hatching of eggs . 20
B.6 Larval development . 20
B.7 Enemies and parasites . 21
Annex C (informative) Differentiation of heartwood and sapwood in Pinus species . 22
C.1 Principle . 22
C.2 Reagents . 22
C.3 Apparatus . 22
C.4 Procedure . 22
Annex D (informative) Environmental, health and safety precautions within
chemical/biological laboratory . 23
Annex E (normative) Slow acting and deferred effect formulations – Extending the test
duration . 24
Bibliography . 26

European foreword
This document (prEN 46-1:2025) has been prepared by Technical Committee CEN/TC 38 “Biological
durability of wood and wood-based products”, the secretariat of which is held by SIS.
This document is currently submitted to the CEN Enquiry.
This document will supersede EN 46-1:2016.
Significant technical differences between this document and EN 46-1:2016 are as follows:
a) specifications for sealing faces of test specimens to be treated with solutions in which an organic
solvent is the continuous phase were generalized (5.2.2);
b) requirements for temperature and humidity were aligned with other insect test standards (5.3.1;
5.3.4; B5; B6);
c) imaging facilities for non-destructive recordings of insect tunnels inside test specimens were
generalized (5.3.11);
d) storage limitations for wood from which test specimens are to be made from were deleted (7.2);
e) a note was added to 8.1.3.1.3 to optionally reduce the number of concentrations which are
necessary to determine the toxic value;
f) Specifications for dipping (8.1.3.3) were generalized and aligned with other insect standards.
NOTE Test results obtained according to earlier versions of this document or when the tests had started
before this version of EN 46-1 was published are considered as valid.
The standard EN 46 is composed of two parts:
— EN 46-1, Wood preservatives ― Determination of the preventive action against recently hatched
larvae of Hylotrupes bajulus (Linnaeus) ― Part 1: Application by surface treatment (laboratory
method)
— EN 46-2, Wood preservatives ― Determination of the preventive action against recently hatched
larvae of Hylotrupes bajulus (Linnaeus) ― Part 2: Ovicidal effect (laboratory method)
EN 46 consists of two parts to enable preventive action of wood preservatives, against recently hatched
larvae of Hylotrupes bajulus, which are intended to be applied by surface treatment; Part 1 is required to
determine the larvicidial effect of preservatives and Part 2 is required to determine the ovicidal action
of the preservatives after egg-laying of young females.

Introduction
This document describes a laboratory method of testing which gives a basis for the assessment of the
preventive action of a wood preservative, when applied as a surface treatment, against recently hatched
larvae of Hylotrupes bajulus, whereas the method for determining the toxic values against Hylotrupes
bajulus (EN 47) provides a means of checking whether a preservative prevents attack by these larvae
and prevents their survival within totally impregnated wood.
This document provides information for the sealing of all but one later
...