CEN ISO/TR 6579-3:2014

Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 3: Guidelines for serotyping of Salmonella spp. (ISO/TR 6579-3:2014)

ISO/TR 6579-3:2014 gives guidance on the procedure for serotyping Salmonella serovars and is applicable to the serotyping of pure cultures of Salmonella spp., independent of the source from which they are isolated.

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis, zur Zählung und zur Serotypisierung von Salmonellen - Teil 3: Leitfaden für die Serotypisierung von Salmonella spp. (ISO/TR 6579-3:2014)

Der vorliegende Teil von ISO 6579 enthält eine Anleitung zur Verfahrensweise bei der Serotypisierung von Salmonellen-Serovaren und gilt für die Serotypisierung von Reinkulturen von Salmonella spp. unabhängig von der Quelle, aus der sie isoliert werden.

Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche, le dénombrement et le sérotypage des Salmonella - Partie 3: Lignes directrices pour le sérotypage des Salmonella spp. (ISO/TR 6579-3:2014)

ISO/TR 6579-3:2014 donne des recommandations relatives au mode opératoire de sérotypage des Salmonella. Il s'applique aux cultures pures de Salmonella spp. indépendamment de l'origine de l'isolement.

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti, števila in serotipov Salmonella - 3. del: Navodila za serotipizacijo Salmonella spp (ISO/TR 6579-3:2014)

Standard CEN ISO/TR 6579-3 določa navodila za serotipizacijo Salmonella spp in se uporablja za serotipizacijo čistih kultur Salmonella spp, neodvisno od vira, iz katerega so bile izolirane.

General Information

• Status: Published
• Publication Date: 15-Jul-2014
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463 - Microbiology of the food chain
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 16-Jul-2014
• Completion Date: 16-Jul-2014
• Ref Project: SIST-TP CEN ISO/TR 6579-3:2014 - Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 3: Guidelines for serotyping of Salmonella spp. (ISO/TR 6579-3:2014)

Relations

• Replaces: EN ISO 6579:2002/AC:2006 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp (ISO 6579:2002/Cor.1:2004)
• Effective Date: 23-Jul-2014
• Replaces: EN ISO 6579:2002/A1:2007 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp. - Amendment 1: Annex D: Detection of Salmonella spp. in animal faeces and in environmental samples from the primary production stage (ISO 6579:2002/Amd 1:2007)
• Effective Date: 23-Jul-2014

Standards Content (Sample)

SLOVENSKI STANDARD
01-november-2014
1DGRPHãþD
SIST EN ISO 6579:2003
SIST EN ISO 6579:2003/A1:2007
SIST EN ISO 6579:2003/AC:2004
SIST EN ISO 6579:2003/AC:2006
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje
prisotnosti, števila in serotipov Salmonella - 3. del: Navodila za serotipizacijo
Salmonella spp (ISO/TR 6579-3:2014)
Microbiology of the food chain - Horizontal method for the detection, enumeration and
serotyping of Salmonella - Part 3: Guidelines for serotyping of Salmonella spp. (ISO/TR
6579-3:2014)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zum
Nachweis, zur Zählung und zur Serotypisierung von Salmonellen - Teil 3: Leitfaden für
die Serotypisierung von Salmonella spp. (ISO/TR 6579-3:2014)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche, le
dénombrement et la sérotypie des Salmonella - Partie 3: Lignes directrices pour la
sérotypie des Salmonella spp. (ISO/TR 6579-3:2014)
Ta slovenski standard je istoveten z: CEN ISO/TR 6579-3:2014
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

TECHNICAL REPORT
CEN ISO/TR 6579-3
RAPPORT TECHNIQUE
TECHNISCHER BERICHT
July 2014
ICS 07.100.30
English Version
Microbiology of the food chain - Horizontal method for the
detection, enumeration and serotyping of Salmonella - Part 3:
Guidelines for serotyping of Salmonella spp. (ISO/TR 6579-
3:2014)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie von Lebensmitteln und Futtermitteln -
horizontale pour la recherche, le dénombrement et la Horizontales Verfahren zum Nachweis, zur Zählung und zur
sérotypie des Salmonella - Partie 3: Lignes directrices pour Serotypisierung von Salmonellen - Teil 3: Leitfaden für die
la sérotypie des Salmonella spp. (ISO/TR 6579-3:2014) Serotypisierung von Salmonella spp. (ISO/TR 6579-3:2014)

This Technical Report was approved by CEN on 28 October 2013. It has been drawn up by the Technical Committee CEN/TC 275.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN ISO/TR 6579-3:2014 E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
This document (CEN ISO/TR 6579-3:2014) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the
secretariat of which is held by DIN.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
Endorsement notice
The text of ISO/TR 6579-3:2014 has been approved by CEN as CEN ISO/TR 6579-3:2014 without any
modification.
TECHNICAL ISO/TR
REPORT 6579-3
First edition
2014-07-15
Microbiology of the food chain —
Horizontal method for the detection,
enumeration and serotyping of
Salmonella —
Part 3:
Guidelines for serotyping of
Salmonella spp.
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche, le dénombrement et la sérotypie des Salmonella —
Partie 3: Lignes directrices pour la sérotypie des Salmonella spp.
Reference number
ISO/TR 6579-3:2014(E)
©
ISO 2014
ISO/TR 6579-3:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.org
Published in Switzerland
ii © ISO 2014 – All rights reserved

ISO/TR 6579-3:2014(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Culture media and sera . 2
5.1 General . 2
5.2 Culture media and reagents . 2
5.3 Antisera . 2
6 Apparatus . 2
7 Sample . 3
8 Taxonomy of Salmonella . 3
8.1 General . 3
8.2 Nomenclature. 3
8.3 Biochemical characteristics . 4
8.4 Antigenic characteristics . 5
9 Procedure for Salmonella serotyping . 7
9.1 General . 7
9.2 Example procedure for serotyping five public health-related Salmonella serovars . 7
10 Quality control .11
11 Reporting .11
Annex A (informative) Composition and preparation of culture media and reagents .13
Annex B (informative) Examples of procedures for serotyping an unknown Salmonella isolate .20
Annex C (informative) Biochemical tests .24
Annex D (informative) Schematic overview for serotyping five important public-health related
Salmonella serovars .26
Annex E (informative) Microtitre plate method for serotyping Salmonella spp.27
Annex F (informative) Examples of procedures for phase inversion .29
Bibliography .32
ISO/TR 6579-3:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
ISO 6579 consists of the following parts, under the general title Microbiology of the food chain —
Horizontal method for the detection, enumeration and serotyping of Salmonella:
1)
— Part 1: Horizontal method for the detection of Salmonella spp.
2)
— Part 2: Enumeration by a miniaturized most probable number technique [Technical Specification]
— Part 3: Guidelines for serotyping of Salmonella spp. [Technical Report]
1) Under preparation. (Revision of ISO 6579:2002)
2) The main element of the series title has been changed since Part 2 was published. It is intended that upon
revision, the main element of the title will be aligned with Part 3.
iv © ISO 2014 – All rights reserved

ISO/TR 6579-3:2014(E)
Introduction
This part of ISO 6579 gives information on the taxonomy of Salmonella spp. and it gives guidance on
serotyping of Salmonella serovars, based on the White–Kauffmann–Le Minor scheme (see Reference [9]).
TECHNICAL REPORT ISO/TR 6579-3:2014(E)
Microbiology of the food chain — Horizontal method
for the detection, enumeration and serotyping of
Salmonella —
Part 3:
Guideline
...