EN ISO 20714:2021

E-liquid - Determination of nicotine, propylene glycol and glycerol in liquids used in electronic nicotine delivery devices - Gas chromatographic method (ISO 20714:2019)

This document specifies an analytical method to quantify the nicotine, propylene glycol and glycerol content in e-liquids by gas chromatography.

E-Liquid - Bestimmung von Nikotin, Propylenglykol und Glycerin in Flüssigkeiten, die in elektronischen Nikotinabgabegeräten verwendet werden - Gaschromatographisches Verfahren (ISO 20714:2019)

Dieses Dokument legt ein Analyseverfahren zur Quantifizierung des Nikotin , Propylenglykol  und Glyceringehalts in E Liquids durch Gaschromatographie fest.

E-liquide - Détermination de la teneur en nicotine, propylène glycol et glycérol dans les liquides utilisés avec les systèmes électroniques de délivrance de nicotine - Méthode par chromatographie en phase gazeuse (ISO 20714:2019)

Le présent document spécifie une méthode d'analyse par chromatographie en phase gazeuse permettant de quantifier la teneur en nicotine, propylène glycol et glycérol dans les e-liquides.

E-tekočine - Ugotavljanje deleža nikotina, propilenglikola in glicerola v tekočinah, ki se uporabljajo v elektronskih napravah za dovajanje nikotina - Metoda plinske kromatografije (ISO 20714:2019)

Ta dokument določa analitično metodo za ugotavljanje deleža nikotina, propilenglikola in glicerola v e-tekočinah s plinsko kromatografijo.

General Information

• Status: Published
• Publication Date: 14-Dec-2021
• Withdrawal Date: 29-Jun-2022
• ICS: 65.160 - Tobacco, tobacco products and related equipment
• Technical Committee: CEN/TC 437 - Electronic cigarettes and e-liquids
• Drafting Committee: CEN/TC 437 - Electronic cigarettes and e-liquids
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Dec-2021
• Due Date: 19-Nov-2023
• Completion Date: 15-Dec-2021
• Ref Project: SIST EN ISO 20714:2022 - E-liquid - Determination of nicotine, propylene glycol and glycerol in liquids used in electronic nicotine delivery devices - Gas chromatographic method (ISO 20714:2019)

Overview

EN ISO 20714:2021 / ISO 20714:2019 specifies a validated analytical procedure for quantifying nicotine, propylene glycol (PG) and glycerol in e-liquids used with electronic nicotine delivery devices (ENDDs) using a gas chromatographic method (GC-FID). The standard defines sample preparation, required reagents and apparatus, calibration practice, chromatographic conditions, and reporting to ensure consistent, comparable results across testing laboratories.

Key topics and technical requirements

• Scope and principle
  • Quantification by capillary gas chromatography with flame ionization detection (GC-FID) using internal standards.
• Reagents and standards
  • High-purity carrier/auxiliary gases (helium or hydrogen; FID hydrogen and air).
  • Solvent: isopropanol (≥99%) for dilution.
  • Reference substances: nicotine (≥98%), propylene glycol (≥99%), glycerol (≥99%).
  • Internal standards suggested: quinaldine, 1,3-butanediol, n‑heptadecane, n‑octadecane (users may assess alternatives).
• Calibration
  • At least five calibration solutions covering expected analyte ranges (example ranges used in the collaborative study: nicotine 0.005–1.5 mg/ml; PG and glycerol 0.12–10 mg/ml).
• Apparatus and column
  • Capillary GC with FID; DB‑ALC1 (30 m × 0.32 mm ID, 1.8 µm) cited as satisfactory. Alternative columns allowed if resolution is adequate.
• Sample handling and procedure
  • Typical test portion: 0.100 g sample diluted with 10 ml isopropanol/internal standard solution; equilibrate to room temperature; store extracts at 2–8 °C if necessary.
  • Example GC conditions provided (oven temperature program, injection volume 1 µl, split 50:1, helium carrier 1.8 ml/min, detector/injection temperatures).
• Quality
  • Monitor internal standard peak consistency; perform repeatability and reproducibility assessments and report results per the standard.

Practical applications

• Routine laboratory testing for e-liquid composition and quality control.
• Regulatory compliance testing and product labelling verification (nicotine strength claims).
• Research and product development (formulation verification, stability studies).
• Forensic and public health labs assessing exposure potential from ENDD products.

Who should use this standard

• Analytical and contract testing laboratories performing e-liquid analysis.
• E-liquid and electronic cigarette manufacturers implementing QC procedures.
• Regulatory authorities and notified bodies overseeing product safety and compliance.
• Research institutions studying aerosol chemistry and nicotine delivery.

Related standards and references

• ISO 13276 (noted in EN ISO 20714 for verifying nicotine purity).
• Developed by ISO/TC 126 (Tobacco and tobacco products) and adopted by CEN/TC 437 (Electronic cigarettes and e-liquids).

Keywords: EN ISO 20714, ISO 20714, e-liquid analysis, gas chromatographic method, GC‑FID, nicotine determination, propylene glycol, glycerol, e-liquids testing, electronic nicotine delivery devices.

Standards Content (Sample)

SLOVENSKI STANDARD
01-marec-2022
E-tekočine - Ugotavljanje deleža nikotina, propilenglikola in glicerola v tekočinah,
ki se uporabljajo v elektronskih napravah za dovajanje nikotina - Metoda plinske
kromatografije (ISO 20714:2019)
E-liquid - Determination of nicotine, propylene glycol and glycerol in liquids used in
electronic nicotine delivery devices - Gas chromatographic method (ISO 20714:2019)
E-Liquids - Bestimmung von Nikotin, Propylenglycol und Glycerin in Flüssigkeiten, die in
elektronischen Nikotinabgabegeräten verwendet werden - Gaschromatographisches
Verfahren (ISO 20714:2019)
E-liquide - Détermination de la teneur en nicotine, propylène glycol et glycérol dans les
liquides utilisés avec les systèmes électroniques de délivrance de nicotine - Méthode par
chromatographie en phase gazeuse (ISO 20714:2019)
Ta slovenski standard je istoveten z: EN ISO 20714:2021
ICS:
65.160 Tobak, tobačni izdelki in Tobacco, tobacco products
oprema and related equipment
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 20714
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2021
EUROPÄISCHE NORM
ICS 65.160
English Version
E-liquid - Determination of nicotine, propylene glycol and
glycerol in liquids used in electronic nicotine delivery
devices - Gas chromatographic method (ISO 20714:2019)
E-liquide - Détermination de la teneur en nicotine, E-Liquids - Bestimmung von Nikotin, Propylenglycol
propylène glycol et glycérol dans les liquides utilisés und Glycerin in Flüssigkeiten, die in elektronischen
avec les systèmes électroniques de délivrance de Nikotinabgabegeräten verwendet werden -
nicotine - Méthode par chromatographie en phase Gaschromatographisches Verfahren (ISO 20714:2019)
gazeuse (ISO 20714:2019)
This European Standard was approved by CEN on 5 December 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20714:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 20714:2019 has been prepared by Technical Committee ISO/TC 126 "Tobacco and
tobacco products” of the International Organization for Standardization (ISO) and has been taken over
as EN ISO 20714:2021 by Technical Committee CEN/TC 437 “Electronic cigarettes and e-liquids” the
secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2022, and conflicting national standards shall be
withdrawn at the latest by June 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 20714:2019 has been approved by CEN as EN ISO 20714:2021 without any modification.

INTERNATIONAL ISO
STANDARD 20714
First edition
2019-08
E-liquid — Determination of
nicotine, propylene glycol and
glycerol in liquids used in electronic
nicotine delivery devices — Gas
chromatographic method
E-liquide — Détermination de la teneur en nicotine, propylène
glycol et glycérol dans les liquides utilisés avec les systèmes
électroniques de délivrance de nicotine — Méthode par
chromatographie en phase gazeuse
Reference number
ISO 20714:2019(E)
©
ISO 2019
ISO 20714:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 20714:2019(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Sampling . 3
8 Procedure. 3
8.1 Test portion . 3
8.2 Setting up the apparatus. 3
8.3 Calibration of the gas chromatograph . 4
8.4 Determination . 4
9 Expression of results . 4
10 Repeatability and reproducibility . 4
11 Test report . 5
Annex A (informative) Example chromatogram . 6
Bibliography . 7
ISO 20714:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on t
...