65.160 - Tobacco, tobacco products and related equipment

This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of cigarette mainstream smoke with the intense smoking regime specified in ISO 20778 by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC-MS) with methanol as extraction solvent. This method is specified using ISO 20778 smoking parameters. NOTE An alternative method for the determination of B[a]P is specified in ISO 23906-2 with a different clean-up using cyclohexane solvent and a shorter total analytical time.

This document specifies a method for the quantification of selected volatile organic compounds (VOCs: 1,3-butadiene, isoprene, acrylonitrile, benzene and toluene) by gas chromatography/mass spectrometry (GC-MS) in mainstream cigarette smoke using ISO 20778 smoking parameters.

This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n-butyraldehyde) as their 2,4-dinitrophenylhydrazones by reversed phase HPLC-UV/DAD in mainstream smoke using an intense smoking regime.

This document specifies reference e-liquids to be used to test emissions generated by electronic cigarettes [1].
This document applies to the reference e-liquids to be used when an electronic cigarette is sold empty, without an e-liquid, and where the product information or instructions for use are not specific in terms of the compositional characteristics of the e-liquid to be used with the device.

This document specifies a method for the quantification of selected phenolic compounds by high performance liquid chromatography with fluorescence detection (HPLC-FLD) using ISO 3308 smoking parameters. The selected phenolic compounds are: hydroquinone, resorcinol, catechol, phenol, p-Cresol, m-Cresol and o-Cresol. This method is applicable to cigarettes with total particulate matter (TPM) yields between 1 mg/cigarette and 16 mg/cigarette.

This document specifies a method for the quantification of ammonia by ion chromatography in mainstream smoke using ISO 20778 smoking parameters. This method is applicable to an ammonia yield of about 10 µg/cigarette to 40 µg/cigarette. It is not applicable for the determination of ammonia in dark-air cured cigarettes.

This document specifies a method for the quantification of selected phenolic compounds by high performance liquid chromatography with fluorescence detection (HPLC-FLD) using ISO 20778 smoking parameters. The selected phenolic compounds are: hydroquinone, resorcinol, catechol, phenol, p-Cresol, m-Cresol and o-Cresol. This method is applicable to cigarettes with total particulate matter (TPM) yields between 20 mg/cigarette and 45 mg/cigarette.

This document specifies a method for the determination of the content of total alkaloids as nicotine in tobacco by continuous-flow analysis. This method is applicable to leaf samples, stems, reconstituted tobacco sheet materials and tobacco blends.

This document specifies additional test methods for routine analytical cigarette-smoking machines intended to check the conformity of these machines with ISO 3308 and ISO 20778.
It only establishes additional test methods for smoking machines and does not deal with actual smoking, which is described in other International Standards.
It is composed of four sections relating to
— the determination of pressure drop (Clause 4);
— the determination of significant puff profile parameters (Clause 5...view more

This document specifies a method for the determination of air permeability (AP).
It is applicable to materials used as cigarette papers, filter plug wrap and filter joining paper, including materials having an oriented permeable zone or discrete permeable zones where the measured permeability is in excess of 10 cm3·(min−1·cm−2) at 1 kPa. In addition, it is applicable to banded cigarette papers, with bands of a width of at least 4 mm.
NOTE For an estimate of the air permeability of materials ou...view more

This document specifies the general principles to be applied when sampling batches of raw tobacco in order to assess either
— the mean value of one or more of its characteristics, or
— the heterogeneity of one or more of its characteristics.
Manufactured tobacco products, including products intended for sale or distribution, are specifically not included in the scope of this document.
This document is applicable to the sampling of batches of raw tobacco of the following types:
a) leaf tobac...view more

This document specifies methods for the determination of total particulate matter and for the subsequent determination of nicotine-free dry particulate matter present in the smoke from cigarettes generated and collected using a routine analytical smoking machine.

This document specifies a method for the gas-chromatographic determination of water in total particulate matter from the mainstream smoke. The smoking of cigarettes and collection of mainstream smoke are normally carried out in accordance with ISO 4387. However, the method of this document is also applicable to the determination of water in total particulate matter from the mainstream smoke obtained by non-standard smoking.
NOTE In countries not in a position to use the gas-chromatographic meth...view more

This document specifies a method for the determination of ventilation which is applicable to cigarettes.

This document specifies a method for the quantification of ammonia by ion chromatography in mainstream smoke using ISO 3308 smoking parameters. This method is applicable to cigarettes with ammonia yields between 1 µg/cigarette and 30 µg/cigarette. It is not applicable for the determination of ammonia in dark-air cured cigarettes.

This document specifies a method for the gravimetric determination of the purity of nicotine using tungstosilicic acid. The method is applicable to pure nicotine or nicotine salts used to calibrate analytical methods for the determination of nicotine in the field of tobacco, tobacco products and smoke analysis.

This document specifies the general principles to be applied when sampling batches of raw tobacco in order to assess either — the mean value of one or more of its characteristics, or — the heterogeneity of one or more of its characteristics. Manufactured tobacco products, including products intended for sale or distribution, are specifically not included in the scope of this document. This document is applicable to the sampling of batches of raw tobacco of the following types: a) leaf tobacco: f...view more

This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/MS) with cyclohexane as extraction solvent. This method is specified using ISO 3308 smoking parameters. This document provides an alternative method to that specified in ISO 22634-1, with a different clean-up, and a shorter total analytical run allowing a potential increase of sample throughput in compa...view more

This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/MS) with methanol as extraction solvent. This method is specified using ISO 3308 smoking parameters. An alternative method for the determination of B[a]P is specified in ISO 22634-2 with a different clean-up using cyclohexane solvent and a shorter total analytical time.