EN ISO 7932:2004

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 °C (ISO 7932:2004)

ISO 7932:2004 specifies a horizontal method for the enumeration of viable Bacillus cereus by means of the colony-count technique at 30 °C. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
In order to have a practicable test method, the confirmatory stage has been restricted to the typical aspect on MYP agar and the haemolysis test. Thus the term presumptive has been introduced in order to acknowledge the fact that the confirmatory stage does not enable the distinction of B. cereus from other closely related but less commonly encountered Bacillus species, such as B. anthracis, B. thuringiensis, B. weihenstephanensis, B. mycoides. An additional motility test may help to differentiate B. cereus from B. anthracis in cases where the presence of the latter is suspected.

Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zur Zählung von präsumtivem Bacillus cereus - Koloniezählverfahren bei 30° C (ISO 7932:2004)

Diese Internationale Norm legt ein horizontales Verfahren zur Zählung vermehrungsfähiger Stämme von präsumtivem Bacillus cereus mittels des Koloniezählverfahrens bei 30 °C fest. Es gilt für
¾   Erzeugnisse, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind, und
¾   Umgebungsproben im Bereich der Herstellung und Behandlung von Lebensmitteln.
ANMERKUNG   Im Sinne der Praktikabilität des Untersuchungsverfahrens beschränkt sich die Bestätigungsunter-suchung auf den Nachweis typischer Aspekte auf MYP-Agar und auf den Hämolysetest. Resultierend daraus wird der Begriff "präsumtiv" verwendet, wodurch anerkannt wird, dass die Bestätigungsuntersuchungen nicht geeignet sind, zwischen B. cereus and den anderen nahe verwandten, aber weniger häufig nachzuweisenden Bazillenarten, wie B. anthracis, B. thuringiensis, B. weihenstephanensis und B. mycoides zu unterscheiden. Wenn die Anwesenheit von B. anthracis vermutet wird, kann eine zusätzliche Mobilitätsprüfung dazu dienen, B. cereus von B. anthracis zu unterscheiden.

Microbiologie des aliments - Méthode horizontale pour le dénombrement de Bacillus cereus présomptifs - Technique par comptage des colonies à 30 °C (ISO 7932:2004)

L'ISO 7932:2004 spécifie une méthode horizontale pour le dénombrement de Bacillus cereus présomptifs revivifiables par comptage des colonies à 30 °C. Elle est applicable aux produits pour l'alimentation humaine et animale; et aux échantillons d'environnement pour la production et la distribution des aliments.
Dans le but de mettre en oeuvre une méthode pratique, l'étape de confirmation consiste uniquement à la caractérisation des colonies sur le milieu MYP et au test de l'hémolyse. Le terme «présomptif» a donc été introduit de façon à souligner le fait que l'étape de confirmation ne permet pas de faire la différence entre B. cereus et d'autres espèces proches mais néanmoins moins rencontrées, comme B. anthracis, B. thuringiensis, B. weihenstephanensis, B. mycoides. Un test de mobilité effectué en plus peut toutefois aider à la différentiation entre B. cereus et B. anthracis, dans les cas où la présence de ce dernier est suspectée.

Mikrobiologija živil in krme - Splošno navodilo za štetje domnevno prisotnih Bacillus cereus - Štetje kolonij pri 30 °C (ISO 7932:2004)

General Information

• Status: Published
• Publication Date: 30-Nov-2004
• Withdrawal Date: 29-Jun-2005
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463 - Microbiology of the food chain
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 12-Jun-2009
• Completion Date: 12-Jun-2009
• Ref Project: SIST EN ISO 7932:2005 - Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 °C (ISO 7932:2004)

Relations

• Replaces: EN ISO 7932:1997 - Microbiology - General guidance for the enumeration of Bacillus cereus - Colony-count technique at 30 °C (ISO 7932:1993, including Technical Corrigendum 1:1997)
• Effective Date: 22-Dec-2008
• Amended By: EN ISO 7932:2004/A1:2020 - Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 degrees C - Amendment 1: Inclusion of optional tests (ISO 7932:2004/Amd 1:2020, Corrected version 2020-08)
• Effective Date: 05-Sep-2018

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 7932:2005
01-junij-2005
1DGRPHãþD
SIST EN ISO 7932:1998
Mikrobiologija živil in krme - Splošno navodilo za štetje domnevno prisotnih
Bacillus cereus - Štetje kolonij pri 30 °C (ISO 7932:2004)
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of
presumptive Bacillus cereus - Colony-count technique at 30 °C (ISO 7932:2004)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zur Zählung
von präsumtivem Bacillus cereus - Koloniezählverfahren bei 30° C (ISO 7932:2004)
Microbiologie des aliments - Méthode horizontale pour le dénombrement de Bacillus
cereus présomptifs - Technique par comptage des colonies a 30 °C (ISO 7932:2004)
Ta slovenski standard je istoveten z: EN ISO 7932:2004
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 7932:2005 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST EN ISO 7932:2005

---------------------- Page: 2 ----------------------

SIST EN ISO 7932:2005



EUROPEAN STANDARD
EN ISO 7932

NORME EUROPÉENNE

EUROPÄISCHE NORM
December 2004
ICS 07.100.01 Supersedes EN ISO 7932:1997
English version
Microbiology of food and animal feeding stuffs - Horizontal
method for the enumeration of presumptive Bacillus cereus -
Colony-count technique at 30 °C (ISO 7932:2004)
Microbiologie des aliments - Méthode horizontale pour le
dénombrement de Bacillus cereus présomptifs - Technique
par comptage des colonies à 30 °C (ISO 7932:2004)
This European Standard was approved by CEN on 17 June 2004.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.

CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.




EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2004 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 7932:2004: E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST EN ISO 7932:2005
EN ISO 7932:2004 (E)


Foreword

This document (EN ISO 7932:2004) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 275 "Food
analysis - Horizontal methods", the secretariat of which is held by DIN.

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by June 2005, and conflicting national standards
shall be withdrawn at the latest by June 2005.

This document supersedes EN ISO 7932 :1997.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Cyprus,
Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

Endorsement notice

The text of ISO 7932:2004 has been approved by CEN as EN ISO 7932:2004 without any
modifications.
2

---------------------- Page: 4 ----------------------

SIST EN ISO 7932:2005


INTERNATIONAL ISO
STANDARD 7932
Third edition
2004-06-15


Microbiology of food and animal feeding
stuffs — Horizontal method for the
enumeration of presumptive Bacillus
cereus — Colony-count technique at
30 °C
Microbiologie des aliments — Méthode horizontale pour le
dénombrement de Bacillus cereus présomptifs — Technique par
comptage des colonies à 30 °C




Reference number
ISO 7932:2004(E)
©
ISO 2004

---------------------- Page: 5 ----------------------

SIST EN ISO 7932:2005
ISO 7932:2004(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be
...