EN 15510:2007
(Main)Animal feeding stuffs - Determination of calcium, sodium, phosphorus, magnesium, potassium, iron, zinc, copper, manganese, cobalt, molybdenum, arsenic, lead and cadmium by ICP-AES
Animal feeding stuffs - Determination of calcium, sodium, phosphorus, magnesium, potassium, iron, zinc, copper, manganese, cobalt, molybdenum, arsenic, lead and cadmium by ICP-AES
This European Standard specifies inductively coupled plasma atomic emission spectroscopy (ICP-AES) method for the determination of:
- minerals calcium, sodium, phosphorus, magnesium and potassium and the elements iron, zinc, copper, manganese, cobalt, molybdenum in animal feeding stuffs,
- elements arsenic, lead and cadmium in minerals on their own, in pre-mixtures or mixtures for use in animal nutrition.
The method detection limit for each element is dependent on the sample matrix as well as of the instrument. The method is not applicable for determination of low concentrations of elements. The limit of quantification should be 3 mg/kg or lower.
NOTE This method can also be used for the determination of minerals in products with high mineral content (> 5%), yet for this purpose, other more precise analytical techniques are available.
Futtermittel - Bestimmung von Calcium, Natrium, Phosphor, Magnesium, Kalium, Eisen, Zink, Kupfer, Mangan, Cobalt, Molybdän, Arsen, Blei und Cadmium mittels ICP-AES
Diese Europäische Norm legt ein Verfahren mittels Atomemissionsspektrometrie mit induktiv gekoppeltem Plasma (ICP AES) zur Bestimmung von
den Mineralstoffen Calcium, Natrium, Phosphor, Magnesium und Kalium und den Elementen Eisen, Zink, Kupfer, Mangan, Cobalt und Molybdän in Futtermitteln,
den Elementen Arsen, Blei und Cadmium in Mineralien für sich allein, in Vormischungen und Mischungen für die Anwendung in Tiernahrung
fest.
Die Nachweisgrenze des Verfahrens hängt bei jedem Element sowohl von der Probenmatrix als auch von dem Gerät ab. Das Verfahren ist nicht für die Bestimmung von geringen Elementkonzentrationen anwendbar. Die Bestimmungsgrenze sollte bei 3 mg/kg oder niedriger liegen.
ANMERKUNG Dieses Verfahren kann für die Bestimmung von Mineralstoffen in Produkten mit einem hohen Mineral¬stoffgehalt (> 5 %) eingesetzt werden, für diesen Zweck stehen aber auch andere genauere Analysenverfahren zur Verfügung.
Aliments des animaux - Détermination des teneurs en calcium, sodium, phosphore, magnésium, potassium, fer, zinc, cuivre, manganèse, cobalt, molybdène, arsenic, plomb et cadmium par ICP-AES
La présente Norme européenne spécifie une méthode de spectrométrie d'émission atomique à plasma à couplage inductif (ICP-AES) pour la détermination de la teneur en :
minéraux (calcium, sodium, phosphore, magnésium et potassium) et en éléments (fer, zinc, cuivre, manganèse, cobalt, molybdène) dans les aliments pour animaux ;
éléments (arsenic, plomb et cadmium) dans des minéraux purs, dans des pré-mélanges ou des mélanges utilisés pour nourrir des animaux.
La limite de détection de la méthode pour chacun des éléments est fonction de la matrice de l'échantillon et de l'instrument. Cette méthode ne permet pas la détermination des éléments en faibles concentrations. Il convient que la limite de quantification soit de 3 mg/kg ou moins.
NOTE La présente méthode peut être utilisée pour la détermination de la teneur en minéraux dans les produits à forte concentration minérale ( 5 %). D'autres techniques d’analyse plus précises sont néanmoins applicables pour ce genre de détermination.
Krma - Določevanje kalcija, natrija, fosforja, magnezija, kalija, železa, cinka, bakra, mangana, kobalta, molibdena, arzena, svinca in kadmija z ICP-AES
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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Animal feeding stuffs - Determination of calcium, sodium, phosphorus, magnesium, potassium, iron, zinc, copper, manganese, cobalt, molybdenum, arsenic, lead and cadmium by ICP-AESAliments des animaux - Détermination des teneurs en calcium, sodium, phosphore, magnésium, potassium, fer, zinc, cuivre, manganese, cobalt, molybdene, arsenic, plomb et cadmium par ICP-AESFuttermittel - Bestimmung von Calcium, Natrium, Phosphor, Magnesium, Kalium, Eisen, Zink, Kupfer, Mangan, Cobalt, Molybdän, Arsen, Blei und Cadmium mittels ICP-AESTa slovenski standard je istoveten z:EN 15510:2007SIST EN 15510:2007en,fr,de65.120KrmilaAnimal feeding stuffsICS:SLOVENSKI
STANDARDSIST EN 15510:200701-november-2007
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 15510August 2007ICS 65.120 English VersionAnimal feeding stuffs - Determination of calcium, sodium,phosphorus, magnesium, potassium, iron, zinc, copper,manganese, cobalt, molybdenum, arsenic, lead and cadmium byICP-AESAliments des animaux - Détermination des teneurs encalcium, sodium, phosphore, magnésium, potassium, fer,zinc, cuivre, manganèse, cobalt, molybdène, arsenic,plomb et cadmium par ICP-AESFuttermittel - Bestimmung von Calcium, Natrium,Phosphor, Magnesium, Kalium, Eisen, Zink, Kupfer,Mangan, Cobalt, Molybdän, Arsen, Blei und Cadmiummittels ICP-AESThis European Standard was approved by CEN on 30 June 2007.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2007 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 15510:2007: E
Results of the interlaboratory test.21 Annex B (informative)
Notes on the detection technique, interferences and quantification.26 Bibliography.29
WARNING – Use of this European Standard can involve hazardous materials, operations and equipment. This standard does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this European Standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. 5 Reagents Use only reagents of recognized analytical grade, unless otherwise specified. 5.1 Water, complying with grade 2 as defined in EN ISO 3696. 5.2 Nitric acid, concentrated, not less than 65 % (mass fraction), having a density of approximately (HNO3) 1,42 g/ml. 5.3 Dilute nitric acid, to be prepared by mixing 1 volume of nitric acid (5.2) with 1 volume of water. 5.4 Nitric acid solution of 5 % (m/v), to be prepared: pipette 160 ml of dilute nitric acid (5.3) into a
1 000 ml volumetric flask (6.7) and fill to the mark with water. 5.5 Nitric acid solution of 2 % (v/v), to be prepared: pipette 20 ml of nitric acid (5.2) into a 1 000 ml volumetric flask (6.7) and fill to the mark with water. 5.6 Hydrochloric acid, concentrated, not less than 30 % (mass fraction), having a density of approximately (HCl) 1,15 g/ml. 5.7 Dilute hydrochloric acid, to be prepared by mixing 1 volume of hydrochloric acid (5.6) with 1 volume of water. 5.8 Hydrochloric acid solution of 1 % (m/v), to be prepared: pipette 60 ml of dilute hydrochloric acid (5.7) into a 1 000 ml volumetric flask (6.7) and fill to the mark with water. 5.9 Element stock solutions Ca, Na, P, Mg, K, Fe, Zn, Cu, Mn, Co, Mo, Cd, Pb, As c = 1 000 mg/l. The user should choose a suitable stock solution. Both single-element stock solutions and multi-element stock solutions with adequate specification stating the acid used and the preparation technique are commercially available. It is advisable to use certified stock solutions.
Other combinations of elements at different concentrations can be used, provided that the element stock solutions (5.9) are diluted with the same acid and equal concentration as the acid in the test solution to a range of standards that covers the concentrations of the elements to be determined. 5.10.2 Multi-element standard solution – Minerals in 1 % HCl c (Ca, Na, P, Mg, K) = 40 mg/l
Pipette 40,0 ml of each element stock solution (Ca, Na, P, Mg, K) (5.9) into a 1 000 ml volumetric flask (6.7). Add 60 ml of dilute hydrochloric acid (5.7). Fill to the mark with water and transfer to a suitable storage bottle. 5.10.3 Multi-element standard solution - Fe, Zn, Cu, Mn, Co, Mo in 1 % HCl c (Fe, Zn, Cu, Mn, Co, Mo) = 50 mg/l Pipette 50,0 ml of each element stock solution (Fe, Zn, Cu, Mn, Co, Mo) (5.9) into a 1 000 ml volumetric flask (6.7). Add 60 ml of dilute hydrochloric acid (5.7). Fill to the mark with water and transfer to a suitable storage bottle. 5.10.4 Multi-element standard solution – Cd, Pb, As in 5 % HNO3 c (Cd, Pb, As) = 100 mg/l
Pipette 100,0 ml of each element stock solution (Cd, Pb, As) (5.9) into a 1000 ml volumetric flask (6.7). Add 160 ml dilute nitric acid (5.3). Fill to the mark with water and transfer to a suitable storage bottle.
6 Apparatus, usual laboratory apparatus and, in particular, the following. 6.1 Laboratory grinder 6.1.1 Use laboratory grinders that are equipped such that they do not lead to contamination of the samples. 6.1.2 Laboratory grinder capable of grinding to a particle size of less than or equal to 1 mm, e.g. a knife mill or equivalent. 6.1.3 Laboratory grinder capable of grinding to a particle size of less than or equal to 0,1 mm, e.g. a ball mill or equivalent. 6.1.4 Mortar with pestle, free of contamination. 6.2 Analytical balance, capable of weighing to an accuracy of 1 mg. 6.3 Electric hot plate, with temperature control. 6.4 Ashing crucibles, of platinum, quartz or porcelain. 6.5 Electric muffle-furnace, capable of being maintained at a temperature of 450 °C ± 20 °C. The real temperature in the furnace has to be checked, because this temperature may be substantially different from the adjust temperature. 6.6 Beaker, of capacities 100 ml, 250 ml.
6.7 One-mark volumetric flasks, of capacities 100 ml, 500 ml, 1 000 ml. 6.8 Inductively coupled plasma – Atomic Emission Spectrometer
The instrument shall be equipped with a radial plasma as a minimum requirement; an axial plasma is equally acceptable. Background correction shall also be performed when necessary. Settings of the working conditions (e.g. viewing height, gas flows, RF or plasma power, sample uptake rate, integration time, number of replicates, …) shall be optimised according the manufacturer’s instructions. 6.9 Freeze drying equipment, capable of freeze-drying liquid animal feeding stuffs. 7 S
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