EN ISO 16712:2006
(Main)Water quality - Determination of acute toxicity of marine or estuarine sediment to amphipods (ISO 16712:2005)
Water quality - Determination of acute toxicity of marine or estuarine sediment to amphipods (ISO 16712:2005)
ISO 16712:2005 specifies a method for the determination of acute toxicity to amphipods exposed over a period of 10 d to samples of contaminated marine or estuarine sediment, to chemical, industrial or municipal sludge or other solid wastes that may combine with marine or estuarine sediments, or to chemicals or preparations spiked into clean sediment.
Wasserbeschaffenheit - Bestimmung der akuten Toxizität mariner Sedimente oder von Sedimenten aus Flussmündungsgebieten gegenüber Amphipoden (ISO 16712:2005)
Diese Internationale Norm legt ein Verfahren zur Bestimmung der akuten Toxizität gegenüber Amphipoden fest, die über einen Zeitraum von 10 Tagen
a) Proben von belasteten marinen Sedimenten oder Sedimenten aus Flussmündungsgebieten,
b) chemischem, industriellem oder kommunalem Klärschlamm oder anderen festen Abfällen, die in marinen Sedimenten oder Sedimenten aus Flussmündungsgebieten vorhanden sein können, oder
c) Chemikalien oder Ansätzen, mit denen unbelastete Sedimenten aufgestockt sind,
ausgesetzt wurden.
Qualité de l'eau - Détermination de la toxicité aiguë des sédiments marins et estuariens vis-à-vis des amphipodes (ISO 16712:2005)
L'ISO 16712:2005 spécifie une méthode de détermination de la toxicité aiguë vis-à-vis des amphipodes exposés pendant une période de 10 jours à des échantillons de sédiment marin ou estuarien pollué, à des boues chimiques, industrielles ou urbaines, ou à d'autres déchets solides susceptibles de s'associer aux sédiments marins ou estuariens, ou à du sédiment propre dopé avec des produits chimiques ou des préparations.
Kakovost vode - Določanje akutne toksičnosti morskih sedimentov ali sedimentov iz rečnih ustij na rake (Amphipoda) (ISO 16712:2005)
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Water quality - Determination of acute toxicity of marine or estuarine sediment to amphipods (ISO 16712:2005)Qualité de l'eau - Détermination de la toxicité aiguë des sédiments marins et estuariens vis-a-vis des amphipodes (ISO 16712:2005)Wasserbeschaffenheit - Bestimmung der akuten Toxizität mariner Sedimente oder von Sedimenten aus Flussmündungsgebieten gegenüber Amphipoden (ISO 16712:2005)13.080.30Biološke lastnosti talBiological properties of soils13.060.10Voda iz naravnih virovWater of natural resourcesICS:SIST EN ISO 16712:2007en,fr,deTa slovenski standard je istoveten z:EN ISO 16712:200601-februar-2007SIST EN ISO 16712:2007SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN ISO 16712October 2006ICS 13.080.30 English VersionWater quality - Determination of acute toxicity of marine orestuarine sediment to amphipods (ISO 16712:2005)Qualité de l'eau - Détermination de la toxicité aiguë dessédiments marins et estuariens vis-à-vis des amphipodes(ISO 16712:2005)Wasserbeschaffenheit - Bestimmung der akuten Toxizitätmariner Sedimente oder von Sedimenten ausFlussmündungsgebieten gegenüber Amphipoden (ISO16712:2005)This European Standard was approved by CEN on 11 September 2006.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2006 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 16712:2006: E
Foreword
The text of ISO 16712:2005 has been prepared by Technical Committee ISO/TC 147 "Water quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 16712:2006 by Technical Committee CEN/TC 230 "Water analysis", the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by April 2007, and conflicting national standards shall be withdrawn at the latest by April 2007.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 16712:2005 has been approved by CEN as EN ISO 16712:2006 without any modifications.
INTERNATIONALSTANDARDISO16712First edition2005-02-15Reference numberISO16712:2005(E)© ISO2005Water quality— Determination of acute toxicity of marine or estuarine sediment to amphipodsQualité de l'eau— Détermination de la toxicité aiguë des sédiments marins et estuariens vis-à-vis des amphipodes
ISO16712:2005(E)ii© ISO2005–All rights reservedPDF disclaimerThis PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shallnot be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. Indownloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariataccepts no liability in this area.Adobe is a trademark of Adobe Systems Incorporated.Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creationparameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In theunlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.©ISO2005All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below orISO's member body in the country of the requester.ISO copyright officeCase postale 56 CH-1211 Geneva 20Tel.+ 41 22 749 01 11Fax+ 41 22 749 09 47E-mailcopyright@iso.orgWebwww.iso.orgPublished in Switzerland
ISO16712:2005(E)© ISO2005–All rights reservediiiContents Page1Scope . 12Principle . 13Test environment . 13.1Facilities . 13.2Lighting . 14Reagents and materials . 14.1Test organism . 14.2Overlying water . 35Apparatus . 46Treatment and preparation of samples . 56.1General . 56.2Control or reference sediment . 56.3Test sediment . 56.4Preparation of sediment samples . 57Test procedures . 67.1Preparing the exposure vessels . 67.2Introducing the organisms . 67.3Test conditions . 77.4Test observations and measurements . 77.5Expression of results . 87.6Reburrowing capability . 87.7Validity of the test . 88Analysis and interpretation of results . 88.1Data analysis . 88.2Non-contaminant factors . 99Reference toxicant . 910Precision . 911Test report . 10AnnexA(informative)Reconstitued salt water . 11AnnexB(normative)Maximum length of amphipod species, optimal salinity and temperatureranges . 12AnnexC(informative)Precision data . 13Bibliography . 14
ISO16712:2005(E)iv© ISO2005–All rights reservedForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies(ISO member bodies). The work of preparing International Standards is normally carried out through ISOtechnical committees. Each member body interested in a subject for which a technical committee has beenestablished has the right to be represented on that committee. International organizations, governmental andnon-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the InternationalElectrotechnical Commission (IEC) on all matters of electrotechnical standardization.International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part2.The main task of technical committees is to prepare International Standards. Draft International Standardsadopted by the technical committees are circulated to the member bodies for voting. Publication as anInternational Standard requires approval by at least 75% of the member bodies casting a vote.Attention is drawn to the possibility that some of the elements of this document may be the subject of patentrights. ISO shall not be held responsible for identifying any or all such patent rights.ISO16712 was prepared by Technical Committee ISO/TC147, Water quality, Subcommittee SC5, Biologicalmethods.
ISO16712:2005(E)© ISO2005–All rights reservedvIntroductionThis International Standard outlines procedures for conducting acute tests for sediment toxicity, using one ormore amphipod species that are found primarily below the sediment surface in coastal marine and estuarinewaters. The biological endpoint for the test is percent mortality at day 10.
.vi
INTERNATIONAL STANDARDISO16712:2005(E)© ISO2005–All rights reserved1Water quality— Determination of acute toxicity of marine or estuarine sediment to amphipods1ScopeThis International Standard specifies a method for the determination of acute toxicity to amphipods exposedover a period of
toa)samples of contaminated marine or estuarine sediment,b)chemical, industrial or municipal sludge, or other solid wastes that may combine with marine or estuarinesediments, orc)chemicals or preparations spiked into clean sediment.2PrincipleMarine or estuarine amphipods which typically live below the sediment surface are exposed for
tocontaminated sediment or to sediment spiked with a test chemical. The endpoint for the test is percent mortality.The test is performed in 1-litre vessels with
of solid-phase sediment and overlying water. Salinity andtemperature are dependent on the species of amphipod used in testing.3Test environment3.1FacilitiesThe test facility shall be well ventilated, isolated from physical disturbances and free from dust and fumes.3.2LightingAll test vessels shall receive direct, overhead illumination that provides normal laboratory lighting (i.e.
to) at the water surface. Illumination should be uniform and shall be continuous throughout the test periodto inhibit the nocturnal migration of amphipods into the water column[39].4Reagents and materials4.1Test organism4.1.1GeneralOne of the marine or estuarine sediment-dwelling amphipod species listed in AnnexB should be used as testorganism for the method in this International Standard. The species identification should be conducted usingtaxonomic keys[18] and confirmed by a qualified taxonomist familiar with identifying marine or estuarineamphipods.10d10d175ml500lx1000lx
ISO16712:2005(E)2© ISO2005–All rights reserved4.1.2Life stage and sizeAmphipods of uniform age and size shall be used for testing and shall not be larger than the maximum allowablespecies size listed in AnnexB. Do not use mature females bearing embryos, nor individuals longer than themaximum length (including antennae) identified in AnnexB, as they might be senescent.4.1.3SourceAll amphipods used in a test shall be derived from the same population and source. Test organisms can beeither recently collected from an area in which contaminants are at or below background levels, or organismscan be cultured in a laboratory [11], [12], [48].4.1.4Collection, handling and transportDepending on species and/or collection site conditions, collect amphipods using a benthic grab1), a smallbiological dredge or, in inter-tidal zones, a shovel. If a dredge is used, a short haul () minimizes damageto the animals[39]. Collect at least one-third more individuals than are required for the test. Choose a collectionsite for which the presence of abundant organisms of the correct size and age has been demonstratedpreviously, or by
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