EN 1528-3:1996

Fatty food - Determination of pesticides and polychlorinated biphenyls (PCBs) - Part 3: Clean-up methods

This part of EN 1528 specifies the details of methods A to H for the clean-up of fats and oils or the isolated fat portion, respectively, using techniques such as liquid/liquid partition, adsorption or gel permeation column chromatography. The applicable usage of the methods A to H is given in detail ineach method described. Note: See also prEN 1528-4 which lists a clean-up procedure for the removal of the bulk of lipids when analysing large quantities of fat.

Fettreiche Lebensmittel - Bestimmung von Pestiziden und polychlorierten Biphenylen (PCB) - Teil 3: Reinigungsverfahren

Dieser Teil von EN 1528 legt Einzelheiten der Verfahren A bis H zur Reinigung von Fetten und Ölen bzw. dem isolierten Fettanteil eines Lebensmittels fest. Dabei werden die Prinzipien der Flüssig/flüssig erteilung, der Adsorptions- oder Gelpermeations-Säulenchromatographie angewandt. Die Anwendungsbereiche der Verfahren A bis H sind jeweils in den einzelnen Verfahren beschrieben.

Aliments gras - Dosage des pesticides et des polychlorobiphényles (PCB) - Partie 3: Méthodes de purification

Cette partie de la norme EN 1528 spécifie les méthodes A à H, pour la purification des corps gras et des huiles ou de la matière grasse extraite à l'aide des techniques telles que la chromatographie de partage, la chromatographie d'adsorption ou la chromatographie par perméation de gel. L'applicabilité de chaque méthode A à H est détaillée pour chacune des méthodes décrites.

Živila, ki vsebujejo maščobe - Določevanje pesticidov in polikloriranih bifenilov (PCB) - 3. del: Metode čiščenja

General Information

• Status: Published
• Publication Date: 19-Nov-1996
• Withdrawal Date: 30-May-1997
• ICS: 67.040 - Food products in general
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 3 - Pesticides and PCB's in foods of animal origin
• Current Stage: 9060 - Closure of 2 Year Review Enquiry - Review Enquiry
• Start Date: 02-Dec-2025
• Completion Date: 02-Dec-2025
• Ref Project: SIST EN 1528-3:1998 - Fatty food - Determination of pesticides and polychlorinated biphenyls (PCBs) - Part 3: Clean-up methods

Relations

• Parent: EN 1528-1:1996 - Fatty food - Determination of pesticides and polychlorinated biphenyls (PCBs) - Part 1: General
• Effective Date: 22-Dec-2008

Overview

EN 1528-3:1996 (published by CEN and transposed as SIST EN 1528-3:1998) is a European standard that defines detailed clean-up methods for the analysis of pesticides and polychlorinated biphenyls (PCBs) in fatty food matrices. This Part 3 of the EN 1528 series specifies the procedures (referred to as methods A to H) used to remove or separate lipids from fats and oils, or from isolated fat fractions, prior to instrumental determination of pesticide and PCB residues.

Key Topics

• Scope and purpose
  • Clean-up of fats and oils or isolated fat portions for pesticide and PCB analysis.
• Clean-up techniques covered
  • Liquid–liquid partition (partitioning between immiscible solvents)
  • Adsorption chromatography (removal of interfering lipids/impurities on adsorbents)
  • Gel permeation (size-exclusion) column chromatography for lipid fractionation
• Methods A–H
  • The standard describes eight named methods (A to H), with the applicability and usage details provided for each method so analysts can select the most appropriate clean-up approach for a given matrix and residue target.
• Analytical context
  • Designed as part of a harmonized approach to pesticide and PCB residue determination in high-fat foods; intended to be used in conjunction with upstream extraction and downstream analysis steps.
• Classification
  • Falls under ICS 67.050 (general methods of tests and analysis for food products).

Applications

• Food testing laboratories performing residue analysis of pesticides and PCBs in fatty foods (e.g., dairy, oils, fatty meat products).
• Regulatory and compliance laboratories implementing standardized methods for monitoring and enforcement.
• Contract testing and quality control in the food industry where reliable lipid removal improves accuracy and reproducibility of residue determinations.
• Environmental and toxicology labs analyzing bioaccumulative contaminants in high-lipid samples.

Practical benefits include improved removal of lipid interferences prior to GC/MS or LC/MS analysis, better reproducibility between labs, and a clearer basis for selecting a clean-up workflow tailored to sample type and analyte class.

Related Standards

• prEN 1528-4 - referenced in EN 1528-3; lists a clean-up procedure for removal of the bulk of lipids when analysing large quantities of fat.
• EN 1528 series - Part 3 is one element of the broader standard series for fatty food pesticide and PCB determination.

Keywords: EN 1528-3, clean-up methods, fatty food, pesticides, PCBs, gel permeation chromatography, liquid–liquid partition, adsorption chromatography, CEN standard.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Fettreiche Lebensmittel - Bestimmung von Pestiziden und polychlorierten Biphenylen (PCB) - Teil 3: ReinigungsverfahrenAliments gras - Dosage des pesticides et des polychlorobiphényles (PCB) - Partie 3: Méthodes de purificationFatty food - Determination of pesticides and polychlorinated biphenyls (PCBs) - Part 3: Clean-up methods67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski
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