EN 12823-2:2000

Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene

This European Standard specifies a method for the determination of total- beta-carotene in foodstuffs by high performance liquid chromatography (HPLC).

Lebensmittel - Bestimmung von Vitamin A mit Hochleistungs-Flüssigchromatographie - Teil 2: Bestimmung von ß-Carotin

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Gesamt- Beta-Carotin in Lebensmitteln mit Hochleistungs-Flüssigchromatographie (HPLC) fest.

Produits alimentaires - Dosage de la vitamine A par chromatographie liquide haute performance - Partie 2: Dosage du sz-carotène

Le présent projet de norme européenne spécifie une méthode de dosage du béta- carotène dans les produits alimentaires par chromatographie liquide haute performance (CLHP)

Živila - Določevanje vitamina A s tekočinsko kromatografijo visoke ločljivosti - 2. del: Merjenja beta karotena

General Information

• Status: Published
• Publication Date: 15-Feb-2000
• ICS: 67.040 - Food products in general
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 9 - Vitamins and Carotenoids
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 03-Sep-2021
• Completion Date: 14-Apr-2025
• Ref Project: SIST EN 12823-2:2000 - Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene

Overview

EN 12823-2:2000 - published by CEN - is a European Standard that specifies a laboratory method for the determination of total β‑carotene in foodstuffs by high performance liquid chromatography (HPLC). Part 2 of the EN 12823 series focuses specifically on measurement of β‑carotene as a component of vitamin A activity in foods. The standard is classified under ICS 67.050 (general methods of tests and analysis for food products) and is also published in national adoption (e.g., SIST EN 12823-2:2000).

Key topics and requirements

The standard sets out the analytical framework and requirements for using HPLC to quantify total β‑carotene in food matrices. Important technical topics typically covered (as indicated by the standard’s scope) include:

• Scope and applicability - defines which foodstuffs and forms of β‑carotene the method addresses.
• Principle of the method - use of HPLC for separation and measurement of β‑carotene.
• Sample preparation and extraction - procedures to obtain carotenoid extracts suitable for chromatographic analysis.
• Chromatographic measurement - requirements for achieving reliable separation and quantification by HPLC.
• Calibration and quantification - use of reference materials or standards and calculation of total β‑carotene results.
• Quality control and validation - performance characteristics, checks for recovery, precision, and repeatability expected of routine testing laboratories.
• Reporting - how results and method conditions should be documented for traceability and regulatory use.

(As this summary is based on the standard’s scope, specific chromatographic parameters, solvents, detectors, or numerical performance limits are not reproduced here.)

Applications

EN 12823-2:2000 is intended for practical use in:

• Food testing laboratories performing compositional and nutritional analyses.
• Food manufacturers verifying β‑carotene content in fortified products and ingredient batches.
• Regulatory bodies and compliance testing assessing label claims for vitamin A precursors.
• Research institutes studying carotenoid content and bioavailability in food matrices.
• Quality assurance activities where standardized, comparable results are required across laboratories.

Using this standard helps ensure consistent, traceable, and comparable determinations of β‑carotene in diverse food products.

Related standards

• EN 12823 series (other parts dealing with vitamin A and carotenoids)
• National adoptions (e.g., SIST EN 12823-2:2000)

Keywords: EN 12823-2:2000, beta-carotene, vitamin A, HPLC, foodstuffs, CEN standard, determination, food analysis, carotenoids, analytical method.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Vitamin A mit Hochleistungs-Flüssigchromatographie - Teil 2: Bestimmung von ß-CarotinProduits alimentaires - Dosage de la vitamine A par chromatographie liquide haute performance - Partie 2: Dosage du sz-caroteneFoodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene67.050Splošne preskusne in analizne metode za živilske pro
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