EN 12822:2014

Foodstuffs - Determination of vitamin E by high performance liquid chromatography - Measurement of α-, β-, γ- and δ-tocopherol

This European Standard specifies a method for the determination of vitamin E in foods by high performance liquid chromatography (HPLC). The determination of vitamin E content is carried out by measurement of α-, β , γ- and δ-tocopherol. This method has been validated in two interlaboratory studies. The first study was for the analysis of α-tocopherol in margarine and milk powder ranging from 9,89 mg/100 g to 24,09 mg/100 g. The second study was for the analysis of α-, β-, γ- and δ-tocopherol in milk powder and of α-, and β-tocopherol in oat powder ranging from 0,057 mg/100 g (β-tocopherol) to 10,2 mg/100 g (α-tocopherol).
NOTE   The vitamin E activity can be calculated from the tocopherol content assuming appropriate factors as given in [1], [2], [3] and [4].

Lebensmittel - Bestimmung von Vitamin E mit Hochleistungs-Flüssigchromatographie - Bestimmung von α-, β-, γ- und δ-Tocopherol

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Vitamin E in Lebensmitteln mit Hochleistungs-Flüssigchromatographie (HPLC) fest. Die Bestimmung des Vitamin E-Gehalts erfolgt anhand der Bestimmung von -, -, - und -Tocopherol. Diese Bestimmung wurde in 2 Ringversuchen validiert. Im ersten Ringversuch wurde auf Tocopherol in Margarine und Milchpulver von 9,89 mg/100 g bis 24,09 mg/100 g untersucht. Im zweiten Ringversuch wurde -, -, - und -Tocopherol in Milchpulver und - und -Tocopherol in Haferpulver von 0,057 mg/100 g (-Tocopherol) bis 10,2 mg/100 g (-Tocopherol) untersucht.
Die Vitamin E-Aktivität kann aus den Tocopherolgehalten unter Verwendung geeigneter Faktoren, auf die in [1], [2], [3] und [4] verwiesen wird, berechnet werden.

Produits alimentaires - Détermination de la teneur en vitamine E par chromatographie liquide haute performance - Dosage des α-, β-, γ- et δ-tocophérols

La présente Norme européenne spécifie une méthode de dosage de la vitamine E présente dans les produits alimentaires par chromatographie liquide haute performance (CLHP). La détermination de la teneur en vitamine E est effectuée par dosage des α-, β-, γ- et δ-tocophérols. Cette méthode a été validée lors de deux essais interlaboratoires. Le premier essai concernait l’analyse de l’α-tocophérol dans la margarine et le lait en poudre, entre 9,89 mg/100 g et 24,09 mg/100 g. Le deuxième essai s’appliquait à l’analyse des α-, β-, γ- et δ-tocophérols dans le lait en poudre et des α- et β-tocophérols dans la poudre d’avoine, entre 0,057 mg/100 g (β-tocophérol) et 10,2 mg/100 g (α-tocophérol).
L’activité de la vitamine E peut être calculée à partir de la teneur en tocophérol, en utilisant les facteurs appropriés tels que mentionnés en [1], [2], [3] et [4].

Živila - Določevanje vitamina E s tekočinsko kromatografijo visoke ločljivosti - Merjenje α-, β-, γ- in δ-tokoferolov

Ta evropski standard določa metodo za določanje vitamina E v živilih s tekočinsko kromatografijo visoke ločljivosti (HPLC). Določanje vsebnosti vitamina E poteka z merjenjem α-, β-, γ- in δ-tokoferolov. Ta metoda je bila validirana z dvema medlaboratorijskima študijama. Prva študija je bila namenjena analizi α-tokoferolov v margarini in mleku v prahu, ki sta vsebovala od 9,89 mg/100 g do 24,09 mg/100 g α-tokoferolov. Druga študija je bila namenjena analizi α-, β-, γ- in δ-tokoferolov v mleku v prahu ter α- in β-tokoferolov v ovsu v prahu, ki sta vsebovala 0,057 mg/100 g β-tokoferolov do 10,2 mg/100 g α-tokoferolov. OPOMBA Aktivnost vitamina E je mogoče izračunati iz količine tokoferolov, če veljajo ustrezni dejavniki iz [1], [2], [3] in [4].

General Information

• Status: Published
• Publication Date: 03-Jun-2014
• ICS: 67.040 - Food products in general
• ICS: 67.050 - General methods of tests and analysis for food products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 9 - Vitamins and Carotenoids
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 28-Oct-2020
• Ref Project: SIST EN 12822:2014 - Foodstuffs - Determination of vitamin E by high performance liquid chromatography - Measurement of α-, β-, γ- and δ-tocopherol

Relations

• Replaces: EN 12822:2000 - Foodstuffs - Determination of vitamin E by high performance liquid chromatography - Measurement of alpha-, beta-, gamma-, and delta-tocopherols
• Effective Date: 16-Jul-2011

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 12822:2014SIST EN 12822:2014en,fr,de01-september-2014SIST EN 12822:2014SLOVENSKI
STANDARDSIST EN 12822:20001DGRPHãþD

EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 12822
June 2014 ICS 67.050 Supersedes EN 12822:2000
English Version
Foodstuffs - Determination of vitamin E by high performance liquid chromatography - Measurement of $-, ß-, - and /-tocopherol
Produits alimentaires - Détermination de la teneur en vitamine E par chromatographie liquide haute performance - Dosage des $-, ß-, - et /-tocophérols
Lebensmittel - Bestimmung von Vitamin E mit Hochleistungs-Flüssigchromatographie - Bestimmung von $-, ß-, - und /-Tocopherol This European Standard was approved by CEN on 17 April 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
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B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 12822:2014 E SIST EN 12822:2014

Examples of HPLC chromatograms . 14 Annex B (informative)
Precision data . 16 Annex C (informative)
Alternative HPLC systems . 18 Bibliography . 19
1) This information is given for convenience of users of this European Standard and does not and does not constitute and endorsement by CEN. Equivalent products may be used if they can be shown to lead to the same results. SIST EN 12822:2014

-tocopherol. If an UV-detector is used to monitor the chromatography, a more concentrated solution shall be used. The standard solution shall be stored protected from light and at a temperature below 4 °C and should be checked as described in 4.10.5. 4.11.2 ptandard solution of a mixture of .--- and i-tocopherol Pipette e.g. 10 ml of each of the stock solutions (4.10) into a one-mark 100 ml volumetric flask and dilute to the mark with the appropriate solvent (for NP e.g. n-hexane, for RP e.g. methanol). The standard solution should have a mass concentration of 1
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