EN ISO 22118:2011

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens - Performance characteristics (ISO 22118:2011)

ISO 22118:2010 specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA or RNA) by molecular methods. ISO 22118:2010 applies to the detection of food-borne pathogens in foodstuffs and isolates obtained from them using molecular detection methods based on the polymerase chain reaction (PCR).
ISO 22118:2010 is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.

Mikrobiologie von Lebensmitteln und Futtermitteln - Polymerase-Kettenreaktion (PCR) zum Nachweis und zur quantitativen Bestimmung von pathogenen Mikroorganismen in Lebensmitteln - Leistungsmerkmale (ISO 22118:2011)

Diese Internationale Norm legt die Mindestanforderungen an die Leistungsmerkmale für den Nachweis von
Nukleinsäuresequenzen (DNS oder RNS) mittels molekularer (Nachweis-)Verfahren fest. Diese Internationale
Norm gilt für den Nachweis von pathogenen Mikroorganismen in Lebensmitteln und aus Lebensmitteln
gewonnenen Isolaten unter Anwendung von molekularen Nachweisverfahren auf der Grundlage der
Polymerase-Kettenreaktion (PCR).
Diese Internationale Norm kann zum Beispiel auch für den Nachweis von über Nahrungsmittel übertragenen
pathogenen Mikroorganismen in Umweltproben oder in Futtermitteln angewendet werden.
ANMERKUNG Auf Grund des schnellen Fortschritts auf diesem Gebiet entsprechen die angegebenen Beispiele den
häufigsten Anwendungen zum Zeitpunkt der Erarbeitung dieser Internationalen Norm.

Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) pour la détection et la quantification des micro-organismes pathogènes dans les aliments - Caractéristiques de performance (ISO 22118:2011)

L'ISO 22118:2010 spécifie les exigences minimales relatives aux caractéristiques de performance pour la détection de séquences d'acide nucléique (ADN ou ARN) par méthodes moléculaires. L'ISO 22118:2010 s'applique à la détection de micro-organismes pathogènes présents dans les aliments et les extraits obtenus à partir de ces aliments en employant des méthodes de détection moléculaires fondées sur la réaction de polymérisation en chaîne (PCR).
L'ISO 22118:2010 peut également être utilisée, par exemple, pour la détection de micro-organismes pathogènes d'origine alimentaire dans des prélèvements environnementaux ainsi que dans les aliments pour animaux.

Mikrobiologija živil in krme - Odkrivanje prisotnosti in kvantifikacija patogenih mikroorganizmov v živilih s polimerazno verižno reakcijo (PCR) - Izvedbena merila (ISO 22118:2011)

Ta mednarodni standard opredeljuje minimalne zahteve za izvedbena merila za odkrivanje prisotnosti zaporedij nukleinskih kislin (DNK ali RNK) z molekularnimi metodami. Ta mednarodni standard se uporablja za odkrivanje prisotnosti patogenih mikroorganizmov v živilih in izolatih, pridobljenih iz njih, z uporabo molekularnih metod odkrivanja prisotnosti, ki temeljijo na polimerazni verižni reakciji (PRC). Ta mednarodni standard se uporablja tudi za odkrivanje na primer prisotnosti patogenih mikroorganizmov v živilih v okoljskih vzorcih in krmah.

General Information

• Status: Published
• Publication Date: 14-Jul-2011
• Withdrawal Date: 30-Jan-2012
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463/WG 1 - General requirements relating to PCR methods
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Jul-2011
• Due Date: 04-Apr-2010
• Completion Date: 15-Jul-2011
• Ref Project: SIST EN ISO 22118:2011 - Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens - Performance characteristics (ISO 22118:2011)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 22118:2011
01-oktober-2011
Mikrobiologija živil in krme - Odkrivanje prisotnosti in kvantifikacija patogenih
mikroorganizmov v živilih s polimerazno verižno reakcijo (PCR) - Izvedbena merila
(ISO 22118:2011)
Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the
detection and quantification of food-borne pathogens - Performance characteristics (ISO
22118:2011)
Mikrobiologie von Lebensmitteln und Futtermitteln - Polymerase-Kettenreaktion (PCR)
zum Nachweis und zur quantitativen Bestimmung von pathogenen Mikroorganismen in
Lebensmitteln - Leistungsmerkmale (ISO 22118:2011)
Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) pour la
détection et la quantification des micro-organismes pathogènes dans les aliments -
Caractéristiques de performance (ISO 22118:2011)
Ta slovenski standard je istoveten z: EN ISO 22118:2011
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 22118:2011 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 22118:2011

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SIST EN ISO 22118:2011


EUROPEAN STANDARD
EN ISO 22118

NORME EUROPÉENNE

EUROPÄISCHE NORM
July 2011
ICS 07.100.30
English Version
Microbiology of food and animal feeding stuffs - Polymerase
chain reaction (PCR) for the detection and quantification of food-
borne pathogens - Performance characteristics (ISO
22118:2011)
Microbiologie des aliments - Réaction de polymérisation en Mikrobiologie von Lebensmitteln und Futtermitteln -
chaîne (PCR) pour la détection et la quantification des
Polymerase-Kettenreaktion (PCR) zum Nachweis und zur
micro-organismes pathogènes dans les aliments - quantitativen Bestimmung von pathogenen
Caractéristiques de performance (ISO 22118:2011) Mikroorganismen in Lebensmitteln - Leistungsmerkmale
(ISO 22118:2011)
This European Standard was approved by CEN on 14 July 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22118:2011: E
worldwide for CEN national Members.

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SIST EN ISO 22118:2011
EN ISO 22118:2011 (E)
Contents Page
Foreword .3
2

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SIST EN ISO 22118:2011
EN ISO 22118:2011 (E)
Foreword
This document (EN ISO 22118:2011) has been prepared by Technical Committee CEN/TC 275 “Food
analysis - Horizontal methods”, the secretariat of which is held by DIN, in collaboration with Technical
Committee ISO/TC 34 “Food products”.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at
the latest by January 2012.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerla
...