EN ISO 18218-1:2023

Leather - Determination of ethoxylated alkylphenols (APEO) - Part 1: Direct method (ISO 18218-1:2023)

This document is a method for determining ethoxylated alkylphenols (APEO) [nonyphenol ethoxylate (NPEOn, where 2 ≤ n ≤ 16) and octylphenol ethoxylate (OPEOn, where 2 ≤ n ≤ 16)] in leather. This direct method is especially suitable when a larger number of leather samples are to be checked for the presence of ethoxylated alkylphenols.
This method requires the use of liquid chromatography (LC) with a triple quadrupole mass spectrometer (MS/MS) to identify and quantify the ethoxylated alkylphenols.
NOTE 1        In the leather industry, the most commonly used ethoxylated alkylphenol is the NPEO, with an average of 9 EO. It has an optimum cloud point in water for the typical leather processing temperatures of 40 °C to 55 °C.
NOTE 2        This document and ISO 18218-2 use different solvents for the extraction of the ethoxylated alkylphenols from leather. Consequently, the two analytical methods are expected to give similar trends but not necessarily the same absolute result for the ethoxylated alkylphenol content in leather.

Leder - Bestimmung von ethoxylierten Alkylphenolen (APEO) - Teil 1: Direktes Verfahren (ISO 18218‑1:2023)

Dieses Dokument legt ein Verfahren zur Bestimmung von ethoxylierten Alkylphenolen (APEO) [Nonylphenolethoxylat (NPEOn, wobei 2 ≤ n ≤ 16) und Octylphenolethoxylat [OPEOn, wobei 2 ≤ n ≤ 16)] in Leder fest. Dieses direkte Verfahren ist besonders geeignet, wenn eine größere Anzahl von Lederproben auf die Anwesenheit von ethoxylierten Alkylphenolen zu überprüfen ist.
Dieses Verfahren erfordert die Anwendung der Flüssigkeitschromatographie (LC) mit einem Triple-Quadrupol-Massenspektrometer (MS/MS), um ethoxylierte Alkylphenole zu identifizieren und quantitativ zu bestimmen.
ANMERKUNG 1   Das in der Lederindustrie am häufigsten verwendete ethoxylierte Alkylphenol ist NPEO mit einem Mittelwert von 9 EO. Es hat bei den typischen Verarbeitungstemperaturen von Leder von 40 °C bis 55 °C einen optimalen Trübungspunkt in Wasser.
ANMERKUNG 2   Dies Dokument und ISO 18218 2 verwenden verschiedene Lösemittel zur Extraktion der ethoxylierten Alkylphenole aus Leder. Für die beiden analytischen Verfahren wird erwartet, dass sie für den Gehalt ethoxylierter Alkylphenole in Leder konsequenterweise ähnliche Tendenzen, jedoch nicht zwangsläufig das absolut identische Ergebnis liefern.

Cuir - Détermination des alkylphénols éthoxylés (APEO) - Partie 1: Méthode directe (ISO 18218-1:2023)

Le présent document spécifie une méthode de détermination des alkylphénols éthoxylés (APEO) [éthoxylate de nonylphénol (NPEOn, où 2 ≤ n ≤ 16) et éthoxylate d’octylphénol (OPEOn, où 2 ≤ n ≤ 16)] dans le cuir. Cette méthode d’analyse directe convient particulièrement lorsque les alkylphénols éthoxylés doivent être dosés sur un grand nombre d’échantillons de cuir.
L’analyse est effectuée par chromatographie en phase liquide (CL) couplée à la spectrométrie de masse en tandem avec un analyseur à triple quadripôle (SM/SM), afin d’identifier et de quantifier les alkylphénols éthoxylés.
NOTE 1        Parmi les alkylphénols éthoxylés, celui qui est le plus utilisé dans l’industrie du cuir est le NPEO, qui contient en moyenne 9 groupes éthoxy. La température de trouble dans l’eau de cette substance est optimale compte tenu des températures généralement utilisées pour le traitement du cuir, qui sont comprises entre 40 °C et 55 °C.
NOTE 2        Le présent document et l’ISO 18218-2 utilisent différents solvants pour l’extraction des alkylphénols éthoxylés du cuir. Par conséquent, on peut s’attendre à ce que les résultats obtenus en suivant l’une des deux méthodes analytiques soient comparables à ceux obtenus en suivant la seconde méthode, mais non nécessairement identiques, pour ce qui concerne la teneur en alkylphénols éthoxylés dans le cuir.

Usnje - Določevanje etoksilatnih alkilfenolov - 1. del: Neposredna metoda (ISO 18218-1:2023)

Standard ISO 18218-1:2015 določa metodo za določevanje etoksilatnih alkilfenolov (nonilfenol etoksilatov [NPEOn z 1 ≤ n ≤ 16] in oktilfenol etoksilatov [OPEOn z 1 ≤ n ≤ 16]) v usnju. Ta neposredna metoda je primerna zlasti, če je treba prisotnost etoksilatnih alkilfenolov preveriti pri večjem številu usnjenih vzorcev.
Ta metoda zahteva uporabo tekočinske kromatografije visoke ločljivosti (HPLC) z masnim spektrometrom s trojnim kvadrupolom (MSMS) za identifikacijo bisfenolov in ugotavljanje njihove količine.
OPOMBA 1: Med komercialnimi etoksilatnimi alkilfenoli se v usnjarski industriji najpogosteje uporablja nonilfenol etoksilat, ki v povprečju vsebuje 9 enot etilenoksida (EO). Njegovo motnišče v vodi je optimalno za tipične temperature pri obdelavi usnja, ki znašajo med 40 °C in 55 °C.
OPOMBA 2: v standardih ISO 18218-1 in ISO 18218-2 se uporabljata različni topili za ekstrakcijo etoksilatnih alkilfenolov iz usnja. Posledično je pričakovati, da bosta analizni metodi pokazali podobne trende, ne pa nujno tudi enakega absolutnega rezultata pri vsebnosti alkilfenoletoksilata v usnju.

General Information

• Status: Published
• Publication Date: 20-Jun-2023
• ICS: 59.140.30 - Leather and furs
• Technical Committee: CEN/TC 289 - Leather
• Drafting Committee: CEN/TC 289/WG 1 - Chemical test methods
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 21-Jun-2023
• Completion Date: 21-Jun-2023
• Ref Project: SIST EN ISO 18218-1:2023 - Leather - Determination of ethoxylated alkylphenols (APEO) - Part 1: Direct method (ISO 18218-1:2023)

Relations

• Replaces: EN ISO 18218-1:2015 - Leather - Determination of ethoxylated alkylphenols - Part 1: Direct method (ISO 18218-1:2015)
• Effective Date: 18-Jan-2023

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2023
Usnje - Določevanje etoksilatnih alkilfenolov - 1. del: Neposredna metoda (ISO
18218-1:2023)
Leather - Determination of ethoxylated alkylphenols (APEO) - Part 1: Direct method (ISO
18218-1:2023)
Leder - Bestimmung von ethoxylierten Alkylphenolen (APEO) - Teil 1: Direktes Verfahren
(ISO 18218-1:2023)
Cuir - Détermination des alkylphénols éthoxylés (APEO) - Partie 1: Méthode directe (ISO
18218-1:2023)
Ta slovenski standard je istoveten z: EN ISO 18218-1:2023
ICS:
59.140.30 Usnje in krzno Leather and furs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 18218-1
EUROPEAN STANDARD
NORME EUROPÉENNE
June 2023
EUROPÄISCHE NORM
ICS 59.140.30 Supersedes EN ISO 18218-1:2015
English Version
Leather - Determination of ethoxylated alkylphenols
(APEO) - Part 1: Direct method (ISO 18218-1:2023)
Cuir - Détermination des alkylphénols éthoxylés Leder - Bestimmung von ethoxylierten Alkylphenolen
(APEO) - Partie 1: Méthode directe (ISO 18218-1:2023) (APEO) - Teil 1: Direktes Verfahren (ISO 18218-
1:2023)
This European Standard was approved by CEN on 16 June 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18218-1:2023 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 18218-1:2023) has been prepared by Technical Committee ISO/IULTCS
"International Union of Leather Technologists and Chemists Societies" in collaboration with Technical
Committee CEN/TC 289 “Leather” the secretariat of which is held by UNI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by December 2023, and conflicting national standards
shall be withdrawn at the latest by December 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 18218-1:2015.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 18218-1:2023 has been approved by CEN as EN ISO 18218-1:2023 without any
modification.
INTERNATIONAL ISO
STANDARD 18218-1
IULTCS
IUC 28-1
Second edition
2023-06
Leather — Determination of
ethoxylated alkylphenols (APEO) —
Part 1:
Direct method
Cuir — Détermination des alkylphénols éthoxylés (APEO) —
Partie 1: Méthode directe
Reference numbers
ISO 18218-1:2023(E)
IULTCS/IUC 28-1:2023(E)
ISO 18218-1:2023(E)
IULTCS/IUC 28-1:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
ISO 18218-1:2023(E)
IULTCS/IUC 28-1:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Apparatus . 2
6 Reagents . 2
7 Sampling . 3
8 Sample preparation and analysis . 3
8.1 Extraction . 3
8.2 Analysis . 4
8.3 Calibration . 4
8.4 Calculation . 4
8.4.1 Determination of the ratio of each APEO congener . 4
8.4.2 Determination of the real concentration of each APEO congener in the
calibration standards . 4
8.4.3 Calibration graph . 4
8.4.4 Calculation of the APEO concentration . 5
9 Test report . 5
Annex A (informative) Example of chromatographic method LC-MS/MS .7
Annex B (informative) Characteristic masses for quantification . 8
Bibliography .10
iii
ISO 18218-1:2023(E)
IULTCS/IUC 28-1:2023(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to
further the advancement of leather science and technology. IULTCS has three Commissions, which
are responsible for establishing international methods for the sampling and testing of leather. ISO
recognizes IULTCS as an international standardizing body for the preparation of test methods for
leather.
This document was prepared by the Chemical Test Commission of the International Union of Leather
Technologists and Chemists Societies (IUC Commission, IULTCS), in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 289, Leather, in accordance with
the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
This second edition cancels and replaces the first edition (ISO 18218-1:2015), which has been technically
revised.
The main changes are as follows:
— a new Clause 3 has been added and subsequent clauses renumbered;
— Clauses 6 and 8 have been technically revised;
— the NOTE in 7.1 of the previous edition has been deleted;
— a new calculation procedure in 8.4 has been added;
— Annex A has been technically revised;
— a new Annex B has been added.
A list of all parts in the ISO 18218 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
ISO 18218-1:2023(E)
IULTCS/IUC 28-1:2023(E)
Introduction
Nonylphenol ethoxylate belongs to the non-ionic surfactants. The biodegradation of nonylphenol
ethoxylate releases the persistent pollutant, the branched nonylphenol. Nonylphenol is a hormonal
acting substance that is toxic for waterborne organisms and many other organisms. For this reason, the
release of nonylphenol ethoxylate into the environment should be avoided.
[4]
In 2003 the European Directive 200
...