Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method)

This European Standard specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment to wood. This method is applicable to: ¾ water-insoluble chemicals which are being studied as active insecticides, ¾ organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates, ¾ organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates, ¾ water-soluble materials, for example salts. NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.

Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven - Teil 1: Oberflächenverfahren (Laboratoriumsverfahren)

Diese Europäische Norm legt ein Verfahren zur Bestimmung der Schutzwirkung oder der Grenze der Wirksamkeit eines Holzschutzmittels gegen den Befall durch Anobium punctatum (De Geer) fest, wenn das Produkt zur Oberflächenbehandlung des Holzes verwendet wird.
Das Verfahren gilt für:
- wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
- organische Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
- organische, in Wasser dispergierbare Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
- wasserlösliche Stoffe, zum Beispiel Salze.
ANMERKUNG   Das Verfahren darf in Verbindung mit einer geeigneten Alterungsbeanspruchung, zum Beispiel nach EN 73, angewendet werden

Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis d'Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des larves - Partie 1: Application par traitement de surface (Méthode de laboratoire)

La présente norme européenne prescrit une méthode de détermination de l'efficacité protectrice ou du seuil d'efficacité d'un produit de préservation du bois contre l’infestation par Anobium punctatum (De Geer) lorsque le produit est appliqué au bois par un traitement de surface.
La présente méthode est applicable :
-   aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides ;
-   aux formules organiques telles qu'elles sont fournies ou préparées en laboratoire par dilution de concentrés ;
-   aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées en laboratoire par dilution de concentrés ; et
-   aux produits solubles dans l’eau, par exemple aux sels.
NOTE   Cette méthode peut être utilisée conjointement avec une épreuve de vieillissement, par exemple l’EN 73.

Biocidni proizvodi za zaščito lesa - Ugotavljanje učinkovitosti preventivne zaščite proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število položenih jajčec in preživelih ličink - 1. del: Površinsko nanašanje (laboratorijska metoda)

Ta evropski standard določa metodo za ugotavljanje učinkovitosti preventivne zaščite ali toksičnih vrednosti zaščitnega sredstva za les proti navadnemu trdoglavcu Anobium punctatum (De Geer), ko je izdelek uporabljen v sklopu površinske obdelave lesa. Ta metoda se uporablja za: ¾ v vodi netopne kemikalije, ki se preučujejo kot aktivni insekticidi, ¾ organske formulacije, ki so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov, ¾ organske formulacije, ki se razpršijo v vodi in so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov, ter ¾ v vodi topne materiale, na primer sol. OPOMBA: ta metoda se lahko uporablja v povezavi s postopkom staranja, na primer EN 73.

General Information

Status
Published
Publication Date
21-Jun-2016
Current Stage
9020 - Submission to 2 Year Review Enquiry - Review Enquiry
Due Date
15-Jul-2021
Completion Date
15-Jul-2021

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SLOVENSKI STANDARD
SIST EN 49-1:2016
01-september-2016
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SIST EN 49-1:2005

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Wood preservatives - Determination of the protective effectiveness against Anobium

punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface

treatment (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium

punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -

Teil 1: Oberflächenverfahren (Laboratoriumsverfahren)

Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis

d'Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des

larves - Partie 1: Application par traitement de surface (Méthode de laboratoire)

Ta slovenski standard je istoveten z: EN 49-1:2016
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
SIST EN 49-1:2016 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 49-1:2016
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SIST EN 49-1:2016
EN 49-1
EUROPEAN STANDARD
NORME EUROPÉENNE
June 2016
EUROPÄISCHE NORM
ICS 71.100.50 Supersedes EN 49-1:2005
English Version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by
egg-laying and larval survival - Part 1: Application by
surface treatment (Laboratory method)

Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden

l'efficacité protectrice vis-à-vis d'Anobium punctatum Wirkung gegenüber Anobium punctatum (De Geer)

(De Geer) par l'observation de la ponte et du taux de durch Beobachten der Eiablage und des Überlebens

survie des larves - Partie 1: Application par traitement von Larven - Teil 1: Oberflächenverfahren

de surface (Méthode de laboratoire) (Laboratoriumsverfahren)
This European Standard was approved by CEN on 5 January 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 49-1:2016 E

worldwide for CEN national Members.
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SIST EN 49-1:2016
EN 49-1:2016 (E)
Contents Page

European foreword ................................................................................................................................................................ 4

Introduction ............................................................................................................................................................................. 5

1 Scope ............................................................................................................................................................................. 6

2 Normative references ............................................................................................................................................. 6

3 Terms and definitions ............................................................................................................................................ 6

4 Principle ...................................................................................................................................................................... 6

5 Test materials ............................................................................................................................................................ 7

5.1 Biological material ................................................................................................................................................... 7

5.2 Products and reagents ............................................................................................................................................ 7

5.3 Apparatus .................................................................................................................................................................... 8

6 Sampling ...................................................................................................................................................................... 8

7 Test specimens .......................................................................................................................................................... 8

7.1 Species of wood ......................................................................................................................................................... 8

7.2 Wood quality .............................................................................................................................................................. 9

7.3 Provision of test specimens .................................................................................................................................. 9

7.4 Dimensions of test specimens ............................................................................................................................. 9

7.5 Number of test specimens ..................................................................................................................................... 9

8 Procedure................................................................................................................................................................. 10

8.1 Preparation of the test specimens .................................................................................................................. 10

8.1.1 Conditioning of test specimens prior to sealing ........................................................................................ 10

8.1.2 Sealing ....................................................................................................................................................................... 10

8.1.3 Treatment of the test specimens ..................................................................................................................... 10

8.1.4 Drying and conditioning of the test specimens after treatment .......................................................... 11

8.2 Exposure of the test specimens to the insects ............................................................................................ 12

8.3 Conditions and duration of the test ................................................................................................................ 12

8.4 Examination of the test specimens ................................................................................................................. 12

9 Validity of test ......................................................................................................................................................... 12

10 Expression of results ............................................................................................................................................ 13

10.1 Assessment of the protective effectiveness ................................................................................................. 13

10.2 Toxic values ............................................................................................................................................................. 13

11 Test report ............................................................................................................................................................... 13

Annex A (informative) Example of a test report ..................................................................................................... 15

Annex B (informative) Identification of sex of test insects Anobium punctatum ........................................ 17

Annex C (informative) Culturing technique for Anobium punctatum ............................................................. 18

C.1 Culture wood ........................................................................................................................................................... 18

C.1.1 Wood species .......................................................................................................................................................... 18

C.1.2 Collection of culture wood ................................................................................................................................. 18

C.1.3 Cutting of culture wood ....................................................................................................................................... 18

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SIST EN 49-1:2016
EN 49-1:2016 (E)

C.1.4 Drying of culture wood ........................................................................................................................................ 18

C.2 Source of beetles .................................................................................................................................................... 18

C.2.1 Collection of beetles .............................................................................................................................................. 18

C.2.2 Quarantine of beetles ........................................................................................................................................... 18

C.3 Infestation of culture wood ................................................................................................................................ 18

C.3.1 Culture vessels ........................................................................................................................................................ 18

C.3.2 Preparation of wood ............................................................................................................................................. 18

C.3.3 Introduction of beetles ........................................................................................................................................ 19

C.4 Culturing conditions ............................................................................................................................................. 19

C.4.1 Normal environment ............................................................................................................................................ 19

C.4.2 Natural pupation induction ............................................................................................................................... 19

C.4.3 Artificial pupation induction ............................................................................................................................. 19

C.5 Collection of beetles .............................................................................................................................................. 19

C.6 General culture hygiene ...................................................................................................................................... 19

Annex D (informative) Environmental, health and safety precautions within

chemical/biological laboratory ........................................................................................................................ 21

Bibliography .......................................................................................................................................................................... 22

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SIST EN 49-1:2016
EN 49-1:2016 (E)
European foreword

This document (EN 49-1:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood

and wood-based products”, the secretariat of which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of an identical

text or by endorsement, at the latest by December 2016, and conflicting national standards shall be

withdrawn at the latest by December 2016.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN 49-1:2005.

Significant technical differences between this document and EN 49-1:2005 are as follows:

a) generalization of material for preparing the egg-laying zones;
b) introduction of new harmonized specifications for wood quality.

This document consists of two parts, Part 1 is required to enable effectiveness assessments of wood

preservatives that are intended to be applied by surface treatment and Part 2 those that are intended to be

applied by impregnation.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following

countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech

Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,

Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,

Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.

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SIST EN 49-1:2016
EN 49-1:2016 (E)
Introduction

This document describes a laboratory method of testing which gives a basis for assessment of the

effectiveness of a wood preservative, when applied as a surface treatment, against Anobium punctatum. It

allows the determination of the concentration at which the product prevents the development of infestation

from egg laying. It can also be used with formulations ready for use.

The method simulates conditions that can occur in practice on timber which has been treated some time

previously with wood preservative applied by dip, brush or spray and on which eggs of Anobium punctatum

are laid.

This laboratory method provides one criterion by which the value of a product can be assessed. In making

this assessment the methods by which the preservative may be applied should be taken into account. It is

further recommended that results from this test should be supplemented by those from other appropriate

tests, and above all by comparison with practical experience.

When products which are very active at low concentrations are used it is very important to take suitable

precautions to isolate and separate, as far as possible, operations involving chemical products, other

products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of

separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for

personnel (see also Annex D for environmental, health and safety precautions).
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SIST EN 49-1:2016
EN 49-1:2016 (E)
1 Scope

This European Standard specifies a method for the determination of the protective effectiveness or the toxic

values of a wood preservative against infestation by Anobium punctatum (De Geer) when the product is

applied as a surface treatment to wood.
This method is applicable to:
— water-insoluble chemicals that are being studied as active insecticides;

— organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;

— organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of

concentrates;
— water-soluble materials, for example salts.

NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.

2 Normative references

The following documents, in whole or in part, are normatively referenced in this document and are

indispensable for its application. For dated references, only the edition cited applies. For undated references,

the latest edition of the referenced document (including any amendments) applies.

EN ISO 835, Laboratory glassware — Graduated pipettes (ISO 835)

EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696)

3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample

sample having its physical or chemical characteristics identical to the volumetric average characteristics of

the total volume being sampled
[SOURCE: EN 1001-2:2005, 4.71]
3.2
supplier

sponsor of the test (person or company providing the sample of wood preservative to be tested)

Note 1 to entry: Adapted from EN 1001–2:2005, 4.83.
4 Principle
Depending on the test being carried out either:

— on a set of test specimens of a susceptible wood species that is surface treated with a solution of the

preservative, or
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SIST EN 49-1:2016
EN 49-1:2016 (E)

— if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that

are surface treated with a series of solutions in which the concentration of preservative is ranged in a

given progression.

The treated test specimens are exposed to gravid females of Anobium punctatum. The number of eggs laid,

the number of eggs hatched and the numbers of surviving larvae are observed and compared with those in

untreated control test specimens. If the preservative has been prepared in the laboratory by dilution of a

concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent

treated control test specimens.
5 Test materials
5.1 Biological material
Anobium punctatum (De Geer)
Adult males and females in good condition.

Adults to be used in the test shall be collected at daily intervals from naturally infested wood or laboratory

culture (see Annex C).

Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.6); and

then checked to ensure that they are undamaged, active and free from any infestation by mites. Determine

the sex (see Annex B) of the collected and checked adults and place the males and females in separate

containers.
NOTE The proportion of males and females varies during the emergence period.
5.2 Products and reagents

5.2.1 Paraffin wax, for sealing the relevant faces of test specimens to be treated with solutions in which

water is the continuous phase.

NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.

5.2.2 Gelatin, for sealing the relevant faces of test specimens to be treated with solutions in which an

organic solvent is the continuous phase.

5.2.3 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and

insoluble in the product under test.
NOTE Sodium carboxy methyl cellulose, food grade, has been found to be suitable.
5.2.4 Water, complying with grade 3 of EN ISO 3696.

5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a

residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the

insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.6 Filter paper, ordinary quality, medium-fast grade.

5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the

egg-laying zones.
NOTE Cotton, linen and polyamide-gauze have been proven as suitable.
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SIST EN 49-1:2016
EN 49-1:2016 (E)
5.3 Apparatus

5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity (80 ± 5)

5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %.

The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that

this has the conditions specified for the conditioning chamber (see 5.3.2).

5.3.3 Treatment vessel, of a material that does not react with the preservative under test, for example of

glass for organic products and of polyethylene for salts containing fluorine.

5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.

CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.

Avoid excessive exposure of operators to solvents or their vapours.

5.3.5 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1).

5.3.6 Pipette, of type specified in EN ISO 835, Class B: graduated pipette with no waiting time. Capacity

1 ml with an accuracy of ± 0,01 ml.

5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to

ensure the safety of the operator.

5.3.8 Test containers, suitable for holding the test specimens and of material resistant to the solvents

used, and fitted with perforated covers to provide a good exchange of air.

NOTE Jars of approximately 60 mm diameter and 100 mm height have been found to be suitable.

5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g.

5.3.10 X-ray apparatus, (optional) with tungsten target and beryllium window, with voltage and current

continuously variable in the ranges:
— voltage: 10 kV to 50 kV;
— current: 0 mA to 15 mA.
5.3.11 Protective gloves
6 Sampling

The sample of preservative shall be representative of the product to be tested. Samples shall be stored and

handled in accordance with any written recommendations from the supplier.

For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.

7 Test specimens
7.1 Species of wood

The reference species is European oak. This shall be either sessile oak (Quercus petraea (Mattuschka)

Lieblin) or pedunculate oak (Quercus robur Linnaeus).
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SIST EN 49-1:2016
EN 49-1:2016 (E)

Additional tests may be carried out using other species but, if so, this should be stated in the test report.

7.2 Wood quality

The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not

have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees

preferably felled in winter. The trees shall be cut immediately after felling and the timber rapidly air-dried or

kiln dried at temperatures below 60 °C. The wood shall not be stored for more than five years.

The wood shall be exclusively sapwood and having between 2 annual rings per 10 mm and 10 annual rings

per 10 mm.

It is recommended to use test specimens of similar growth rate within a single test.

7.3 Provision of test specimens

Prepare planed strips having a cross-section of (25 ± 0,5) mm x (15 ± 0,5) mm removing a minimum of

2 mm from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction of the

grain. The annual rings shall be parallel to the broad faces (contact angle of less than 5°). Make transverse

cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give test specimens

(50 ± 0,5) mm long.

The specimens shall originate from a minimum of three trees or shall be taken at random from a stock

originally of more than 500 test specimens.
7.4 Dimensions of test specimens

The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2) shall be

(50 ± 0,5) mm x (25 ± 0,5) mm x (15 ± 0,5) mm.
Mark each test specimen so that it can be identified throughout the test.
7.5 Number of test specimens
Use:
a) five test specimens (see 7.4) for each preservative and each concentration;

b) five untreated control test specimens (see 7.4) for a complete test of any given preservative;

c) five control test specimens (7.4) treated with that solvent or diluent (5.2.4 or 5.2.5) if a solvent or

diluent (water included) is used.

When dipping is to be used (8.1.3.2.1) it is advisable to treat more than the specified number of test

specimens so that, after weighing, any test specimens with abnormally high or low retentions can be rejected

from the batch.

The growth of young larvae of Anobium punctatum is slow in specimens from resinous wood. Results from test specimens in

resinous wood should be compared with those obtained from oak specimens.
It is not essential in this test for the starch content of the wood to be high.

These test specimens may be taken from the trunk of the tree or the large branches.

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SIST EN 49-1:2016
EN 49-1:2016 (E)
8 Procedure
8.1 Preparation of the test specimens
8.1.1 Conditioning of test specimens prior to sealing

Allow the test specimens to condition in the conditioning chamber (5.3.2), for a minimum of two weeks.

8.1.2 Sealing
8.1.2.1 General

Sealing of the narrower longitudinal and the transverse faces and one of the large faces of the test specimens.

Seal these faces as follows:

8.1.2.2 For tests with solutions in which water is the continuous phase, apply three coats of the

paraffin wax (5.2.1) at about 90 °C so that the first coat adheres closely to the wood and the successive

coatings bond to one another. Condition the sealed test specimens in the conditioning chamber (5.3.2) for at

least one day.

8.1.2.3 For tests with preservative solutions in which the continuous phase is an organic solvent

that dissolves paraffin wax, use the gelatine (5.2.2): apply the first coat as an aqueous solution of 200 g/l at

40 °C, then after a minimum of 8 h of drying, apply two further coats of an aqueous solution of 300 g/l at

50 °C. Condition the sealed test specimens in the conditioning chamber (5.3.2) for at least one day.

8.1.3 Treatment of the test specimens
8.1.3.1 Preparation of treatment solutions
8.1.3.1.1 Solid preservatives

— Water-soluble preservatives: dissolve the preservative in the water (5.2.4) to the required

concentration, or in a series of concentrations if toxic values are to be determined;

— Non-water-soluble preservatives: dissolve the preservative in an appropriate solvent (5.2.5) to the

required concentration, or in a series of concentrations if toxic values are to be determined.

All treatment solutions shall be freshly prepared.
8.1.3.1.2 Liquid preservatives

If appropriate, use the preservative without further preparation other than any necessary stirring. If it is a

concentrate or if toxic values are to be determined, dilute the preservative with the diluent to the required

working concentration, using the procedure specified by the manufacturer. All treatment solutions shall be

freshly prepared.
8.1.3.1.3 Toxic values

If toxic values are to be determined, prepare a series of at least five concentrations by mass, distributed

evenly about the expected toxic values.

A solvent or diluent control, i.e. treatment at concentration = 0, shall also be used. If the approximate toxic

values are unknown, the concentrations shall form a widely spaced geometric progression for a first test and

a more closely spaced geometric or arithmetic progression for subsequent tests.
All treatment solutions shall be freshly prepared.
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SIST EN 49-1:2016
EN 49-1:2016 (E)
8.1.3.2 Application of the treatment solution
8.1.3.2.1 Treatment by dipping

Weigh to the nearest 0,05 g each sealed test specimen, to obtain its initial mass.

Treat each test specimen in the treatment vessel (5.3.3) as follows:
— immerse completely in the solution for 1 min;

— remove and lightly blot test specimens on absorbent paper to remove free fluid from the faces;

— immediately weigh to the nearest 0,05 g.

In the case of water-soluble chemicals, for example salts, and water-insoluble chemicals which are being

studied as active insecticides, calculate the mass of chemical retained for each test specimen from the mass

of solution absorbed and its concentration.

In the case of organic formulations or organic water-dispersible formulations, the retention is expressed for

each test specimen in terms of the corresponding mas
...

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