EN ISO 11733:2004

Water quality - Determination of the elimination and biodegradability of organic compounds in an aqueous medium - Activated sludge simulation test (ISO 11733:2004)

ISO 11733:2004 specifies a method for the determination of the elimination and the biodegradability of organic compounds by aerobic micro-organisms. The conditions described simulate a wastewater treatment plant. Two test systems can be used: activated sludge plants or porous pots. The tests can optionally be performed under conditions of nitrification and denitrification and coupling of the units.
The method applies to organic compounds which, under the conditions of the test, are
soluble in tap water at the test concentration and not expected to be transformed to insoluble metabolites if biodegradation, in addition to elimination, is determined;poorly water-soluble, but which are satisfactorily dispersible in water and allow detection with suitable analytical means (e.g. organic carbon measurements);non-volatile, or which have a negligible vapour pressure under the test conditions;not inhibitory to the test micro-organisms at the concentration chosen for the test. Compounds inhibitory at concentrations used in this test may be tested at concentrations less than their EC20 value, followed by higher practical concentrations after a period of acclimatization.
The method can also be used to measure the biodegradation and elimination of dissolved organic compounds in waste water (also called "test compound" in the method).

Wasserbeschaffenheit - Bestimmung der Elimination und der biologischen Abbaubarkeit organischer Verbindungen in einem wässrigen Medium - Belebtschlamm-Simulationtest (ISO 11733:2004)

Diese Internationale Norm legt ein Verfahren zur Bestimmung der Elimination und der biologischen Abbaubarkeit von organischen Substanzen durch aerobe Mikroorganismen bei einer vorgegebenen Konzentration fest. Die beschriebenen Testbedingungen simulieren eine Kläranlage. Zwei Testsysteme können verwendet werden, die Belebtschlammanlage oder der Poröse Topf. Die Prüfungen können optional unter nitrifizierenden/denitrifizierenden Bedingungen (siehe Anhang A) und unter Kopplung der Anlagen (siehe Anhang B) durchgeführt werden.
Das Verfahren ist unter den gegebenen Testbedingungen für organische Stoffe anwendbar, die
a)   bei der gewählten Testkonzentration wasserlöslich sind, wenn davon ausgegangen werden kann, dass keine unlöslichen Metabolite entstehen, sofern zusätzlich zur Elimination die Abbaubarkeit bestimmt wird;
b)   schwer wasserlöslich, aber ausreichend in Wasser dispergierbar sind und eine Bestimmung mit geeigneten analytischen Verfahren (z. B. Messung des organischen Kohlenstoffs) möglich ist;
c)   nicht flüchtig sind oder einen vernachlässigbaren Dampfdruck besitzen;
d)   bei der gewählten Testkonzentration nicht hemmend gegenüber den Mikroorganismen des Inokulums wirken. Eine Hemmung kann mit einem geeigneten Verfahren (siehe z. B. ISO 8192 [15] oder ISO 15522 [27]) ermittelt werden. Stoffe, die bei der üblichen Testkonzentration hemmend wirken, können zunächst in einer Konzentration zudosiert werden, die unter ihrer EC20 liegt und nach Anpassung auf eine höhere, praktikablere Testkonzentration umgestellt werden.
Das Verfahren kann auch verwendet werden, um die biologische Abbaubarkeit und Elimination von gelösten organischen Komponenten in Abwasser zu bestimmen, die hier ebenfalls "Prüfsubstanz" genannt werden.
Wenn weitere oder zusätzliche Informationen erforderlich sind, um das Verhalten von Prüfsubstanzen oder Abwässern in einer Kläranlage vorherzusagen, können auch andere Abbautests durchgeführt werden. Einen Überblick über die Verfahren und ihre Anwen

Qualité de l'eau - Détermination de l'élimination et de la biodégradabilité des composés organiques en milieu aqueux - Essai de simulation des boues activées (ISO 11733:2004)

L'ISO 11733:2004 spécifie une méthode pour la détermination de l'élimination et de la biodégradabilité des composés organiques sous l'action de micro-organismes aérobies. Les conditions décrites simulent une installation de traitement des eaux usées. Deux systèmes d'essai peuvent être utilisés: les unités de traitement utilisant des boues activées ou les dispositifs à cylindres poreux. Les essais peuvent, de façon facultative, être réalisés dans des conditions de nitrification et de dénitrification, et dans des conditions de couplage des unités d'essai.
La méthode s'applique aux composés organiques qui, dans les conditions d'essai, sont
solubles dans l'eau à la concentration d'essai choisie et non susceptibles d'être transformés en métabolites insolubles, s'il faut déterminer la biodégradation en supplément de l'élimination;faiblement solubles, mais aisément dispersables dans l'eau et pouvant être détectés par des moyens d'analyse appropriés (par exemple mesurages du carbone organique);non volatils ou ayant une pression de vapeur négligeable dans les conditions de l'essai;sans effets inhibiteurs vis-à-vis des micro-organismes à expérimenter à la concentration choisie pour l'essai. Des composés à effets inhibiteurs aux concentrations utilisées pour cet essai peuvent être testés à des concentrations inférieures à la valeur de leur EC20, puis à des concentrations pratiques supérieures après une période d'acclimatation.
La méthode peut également être utilisée pour mesurer la biodégradation et l'élimination des composés organiques dissous dans les eaux usées (également appelés «composés à expérimenter» dans la méthode).

Kakovost vode - Ugotavljanje odstranjevanja in biorazgradljivosti organskih snovi v vodi - Simulacijski preskus z aktivnim blatom (ISO 11733:2004)

General Information

• Status: Published
• Publication Date: 31-Jul-2004
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 14-May-2009
• Completion Date: 14-May-2009
• Ref Project: SIST EN ISO 11733:2005 - Water quality - Determination of the elimination and biodegradability of organic compounds in an aqueous medium - Activated sludge simulation test (ISO 11733:2004)

Relations

• Replaces: EN ISO 11733:1998 - Water quality - Evaluation of the elimination and biodegradability of organic compounds in an aqueous medium - Activated sludge simulation test (ISO 11733:1995)
• Effective Date: 22-Dec-2008

Standards Content (Sample)

SLOVENSKI STANDARD
01-februar-2005
1DGRPHãþD
SIST EN ISO 11733:2000
Kakovost vode – Ugotavljanje odstranjevanja in biorazgradljivosti organskih snovi
v vodi – Simulacijski preskus z aktivnim blatom (ISO 11733:2004)
Water quality - Determination of the elimination and biodegradability of organic
compounds in an aqueous medium - Activated sludge simulation test (ISO 11733:2004)
Wasserbeschaffenheit - Bestimmung der Elimination und der biologischen Abbaubarkeit
organischer Verbindungen in einem wässrigen Medium - Belebtschlamm-Simulationtest
(ISO 11733:2004)
Qualité de l'eau - Détermination de l'élimination et de la biodégradabilité des composés
organiques en milieu aqueux - Essai de simulation des boues activées (ISO 11733:2004)
Ta slovenski standard je istoveten z: EN ISO 11733:2004
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 11733
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2004
ICS 13.060.70 Supersedes EN ISO 11733:1998
English version
Water quality - Determination of the elimination and
biodegradability of organic compounds in an aqueous medium -
Activated sludge simulation test (ISO 11733:2004)
Qualité de l'eau - Détermination de l'élimination et de la
biodégradabilité des composés organiques en milieu
aqueux - Essai de simulation des boues activées (ISO
11733:2004)
This European Standard was approved by CEN on 29 July 2004.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2004 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11733:2004: E
worldwide for CEN national Members.

Foreword
This document (EN ISO 11733:2004) has been prepared by Technical Committee ISO/TC 147
"Water quality" in collaboration with Technical Committee CEN/TC 230 "Water analysis", the
secretariat of which is held by DIN.

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by February 2005, and conflicting national
standards shall be withdrawn at the latest by February 2005.

This document supersedes EN ISO 11733:1998.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

Endorsement notice
The text of ISO 11733:2004 has been approved by CEN as EN ISO 11733:2004 without any
modifications.
INTERNATIONAL ISO
STANDARD 11733
Second edition
2004-08-01
Water quality — Determination of the
elimination and biodegradability of
organic compounds in an aqueous
medium — Activated sludge simulation
test
Qualité de l'eau — Détermination de l'élimination et de la
biodégradabilité des composés organiques en milieu aqueux — Essai
de simulation des boues activées

Reference number
ISO 11733:2004(E)
©
ISO 2004
ISO 11733:2004(E)
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ii © ISO 2004 – All rights reserved

ISO 11733:2004(E)
Contents Page
Foreword. iv
1 Scope. 1
2 Normative references . 1
3 Terms and definitions. 2
4 Principle . 3
5 Test environment . 4
6 Reagents . 4
7 Apparatus. 7
8 Procedure. 8
8.1 General. 8
8.2 Preparation of the inoculum . 8
8.3 Performance of the test. 8
9 Calculation and expression of results . 11
9.1 Calculation of the degree of elimination. 11
9.2 Expression of results. 12
9.3 Indication of biodegradation. 12
9.4 Biodegradation of the organic medium .12
10 Validity of the test . 12
11 Test report. 13
Annex A (informative) Modification of the activated sludge simulation test for nitrifying-
denitrifying sewage treatment plants . 14
Annex B (informative) Coupling of the test units (optional) . 18
Annex C (informative) Test systems. 19
Annex D (informative) Effects of sludge retention time on effluent concentration. 22
Annex E (informative) Example of an elimination/degradation curve. 25
Bibliography . 26

ISO 11733:2004(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 11733 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological
methods.
This second edition cancels and replaces the first edition (ISO 11733:1995), which has been technically
revised.
iv © ISO 2004 – All rights reserved

INTERNATIONAL STANDARD ISO 11733:2004(E)

Water quality — Determination of the elimination and
biodegradability of organic compounds in an aqueous
medium — Activated sludge simulation test
WARNING AND SAFETY PRECAUTIONS — Activated sludge and sewage contain potentially
pathogenic organisms, therefore appropriate precautions should be taken when handling them. Toxic
test compounds and those whose properties are unknown should be handled with care.
1 Scope
This International Standard specifies a method for the determination of the elimination and the
biodegradability of organic compounds by aerobic micro-organisms. The conditions described simulate a
waste-water treatment plant. Two test systems can be used: activated sludge plants or porous pots. The tests
can optionally be performed under conditions of nitrification and denitrification (Annex A) and coupling of the
units (Annex B).
The method applies to organic compounds which, under the conditions of the test, are
a) soluble in tap water at the test concentration and not expected to be transformed to insoluble metabolites
if biodegradation, in addition to elimination, is determined;
b) poorly water-soluble, but which are satisfactorily dispersible in water and allow detection with suitable
analytical means (e.g. organic carbon measurements);
c) non-volatile, or which have a negligible vapour pressure under the test conditions;
d) not inhibitory to the test micro-organisms at the concentration chosen for the test. Inhibitory effects can be
[15] [27]
determined by using a suitable test method (e.g. ISO 8192 or ISO 15522 ). Compounds inhibitory at
concentrations used in this test may be tested at concentrations less than their EC value, followed by
higher practical concentrations after a period of acclimatization.
The method can also be used to measure the biodegradation and elimination of dissolved organic compounds
in waste water (also called “test compound” in the method).
If more or different information is required to predict the behaviour of test compounds or waste water in a
treatment plant, other degradation tests may be performed. For appropriate use of this method and for
alternative biodegradation methods, see ISO/TR 15462 and for general information on biotesting, see
ISO 5667-16.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 5667-16, Water quality — Sampling — Part 16: Guidance on biotesting of samples
ISO 10634, Water quality — Guidance for the preparation and treatment of poorly water-soluble organic
compounds for the subsequent evaluation of their biodegradability in an aqueous medium
ISO/TR 15462, Water quality — Selection of tests for biodegradability
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