13.060.70 - Examination of biological properties of water

This document is focused on the structural features of rivers, on geomorphological and hydrological processes, and on river continuity. It provides guidance on the features and processes to be taken into account when characterizing and assessing the hydromorphology of rivers. It is based on methods developed, tested, and compared in Europe. Its main aim is to improve the comparability of hydromorphological assessment methods, data processing and interpretation. Although it has particular importa...view more

This document specifies a method for determining the toxicity of environmental samples on growth, fertility and reproduction of Caenorhabditis elegans. The method applies to contaminated whole freshwater sediment (maximum salinity 5 g/l), soil and waste, as well as to pore water, elutriates and aqueous extracts that were obtained from contaminated sediment, soil and waste.

This standard specifies a method for the detection, semi-quantitative and quantitative (MPN) enumeration of thermotolerant Campylobacter species. The method can be applied to all kinds of waters including: drinking water, ground water and well water, fresh, brackish and saline surface water, swimming pools, spa and hydrotherapy pools, recreational waters, agricultural waters and runoff, untreated and treated wastewater and also sand and other sediments. This method can be used for the detection ...view more

This document provides guidance for survey design, equipment specification, survey methods, sampling and data handling of macroalgae and marine angiosperms such as Zostera in the intertidal soft bottom environment. It does not include polyeuryhaline terrestrial angiosperms that are found in saltmarshes. Ruppia is a genus of angiosperms that can be found in brackish water. This document can also be applied to the study of Ruppia in these environments.
The document comprises:
-   development of a ...view more

This document provides guidance for survey design, equipment specification, survey methods, sampling and data handling of macroalgae and marine angiosperms such as Zostera in the intertidal soft bottom environment. It does not include polyeuryhaline terrestrial angiosperms that are found in saltmarshes. Ruppia is a genus of angiosperms that can be found in brackish water. This document can also be applied to the study of Ruppia in these environments.
The document comprises:
-   development of a ...view more

This document specifies a method for the detection and quantification of Legionella spp. and
L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general
methodological requirements, performance evaluation requirements, and quality control requirements.
Technical details specified in this document are given for information only. Any other technical
solutions complying with the performance requirements are suitable.
NOTE 1 For performance requirements, see Claus...view more

This technical report specifies the data and metadata necessary to validate the identity of a diatom barcode along with recommendations for storage of the barcode and metadata to ensure access to this information.

This technical report specifies a method for the field sampling of benthic diatoms which will be then analysed by subsequent metabarcoding techniques for ecological status and water quality assessments. Data produced by this method are suitable for production of taxonomical diatom lists.

This document specifies a method for the sampling of mesozooplankton from marine and brackish waters using mesh.

This European standard specifies a procedure for analysing mesozooplankton in marine and brackish waters. The procedures comprise how to identify and enumerate zooplankton to estimate quantitative information on diversity, abundance and biomass with regard to spatial distribution and long-term temporal trends for a given body of water.

This document specifies procedures for sampling of mesozooplankton using nets and continuous ribbon-sampling devices in marine and brackish waters for the purpose of water quality assessment and determination of ecological status of ecosystems.
Guidance on sampling procedures and the subsequent steps of preservation and storage are given. The sampling procedures allow estimates of species occurrence and their abundance (relative or absolute), including spatial distribution and seasonal and long...view more

This document specifies a method for the enumeration of Pseudomonas aeruginosa in water. The
method is based on the growth of target organisms in a liquid medium and calculation of the most
probable number (MPN) of organisms by reference to MPN tables.
This document is applicable to a range of types of water. For example, hospital waters, drinking water
and non‐carbonated bottled waters intended for human consumption, groundwater, swimming pool
and spa pool waters including those containing...view more

This document specifies techniques for preparing poorly water-soluble organic compounds (i.e. liquid
and solid compounds) with a solubility in water of less than approximately 100 mg/l and introducing
them into test vessels for a subsequent biodegradability test in an aqueous medium using standard
methods.
The subsequent tests on biodegradability are primarily methods using the analysis of the released
carbon dioxide described in ISO 9439 and the determination of the oxygen described in ISO...view more

This standard gives guidance on the estimation of abundance and identification of macro-invertebrates in samples taken from inland waters. The procedure deals with pre-treatment (cleaning), sub-sampling, sorting and final identification of organisms from preserved and live samples originating from natural habitats or artificial substrates.

ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B-11177). This part of ISO 11348 specifies a method using freshly prepared bacteria. The method is applicable to waste water, aqueous extracts and leachates, fresh water (surface and ground water), sea and brackish water, eluates of sediment (fresh water, brackish and sea water), pore water, singles substances, diluted in water.

This document specifies a procedure for analysing mesozooplankton in marine and brackish waters. The procedure comprises how to identify and enumerate mesozooplankton to estimate quantitative information on diversity, abundance and biomass with regard to spatial distribution and long-term temporal trends for a given body of water.

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed c...view more