EN ISO 8692:2012
(Main)Water quality - Fresh water algal growth inhibition test with unicellular green algae (ISO 8692:2012)
Water quality - Fresh water algal growth inhibition test with unicellular green algae (ISO 8692:2012)
ISO 8692:2012 specifies a method for the determination of the growth inhibition of unicellular green algae by substances and mixtures contained in water or by waste water. This method is applicable for substances that are easily soluble in water.
With modifications to this method, as specified in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, heavy metals and waste water can be tested.
A rapid algal growth inhibition screening test for waste water is described in an annex.
An alternative test procedure with algae from algal beads, with direct measurement of algal growth in spectrophotometric cells, is described in an annex.
Wasserbeschaffenheit - Süßwasseralgen-Wachstumshemmtest mit einzelligen Grünalgen (ISO 8692:2012)
Diese Internationale Norm legt ein Verfahren zur Bestimmung der Wachstumshemmung von einzelligen
Grünalgen durch Substanzen und Substanzmischungen in Wasser oder durch Abwasser fest. Dieses
Verfahren wird für in Wasser leicht lösliche Stoffe eingesetzt.
Unter Berücksichtigung der in ISO 14442 und ISO 5667-16 beschriebenen Modifikationen können auch die
wachstumshemmenden Effekte von schwer löslichen organischen und anorganischen Stoffen, von flüchtigen
Verbindungen, Schwermetallen und Abwasser untersucht werden.
Ein Screening-Verfahren zur Bestimmung der Wachstumshemmung von Algen in Abwasser siehe Anhang A.
Für ein alternatives Testverfahren mit Algen aus Algenperlen mit direkter Messung des Algenwachstums in
Spektralphotometer-Küvetten siehe Anhang B.
Qualité de l'eau - Essai d'inhibition de la croissance des algues d'eau douce avec des algues vertes unicellulaires (ISO 8692:2012)
L'ISO 8692:2012 spécifie une méthode de détermination de l'inhibition de la croissance des algues vertes unicellulaires par des substances et des mélanges contenus dans l'eau ou par des eaux résiduaires. Cette méthode peut être utilisée avec des substances aisément solubles dans l'eau.
En apportant à cette méthode les modifications spécifiées dans l'ISO 14442 et l'ISO 5667-16, il est possible d'évaluer les effets inhibiteurs des substances inorganiques et organiques faiblement solubles, des composés volatils, des métaux lourds et des eaux résiduaires.
L'Annexe A décrit un essai de détection rapide de l'inhibition de la croissance des algues par des eaux résiduaires.
L'Annexe B décrit un mode opératoire alternatif utilisant des algues provenant de billes d'alginate avec mesurage direct de la croissance algale dans des cellules spectrophotométriques.
Kakovost vode - Preskus zaviranja rasti sladkovodnih alg z enoceličnimi zelenimi algami (ISO 8692:2012)
Ta mednarodni standard določa metodo za določevanje zaviranja rasti enoceličnih zelenih alg zaradi snovi in zmesi, ki jih vsebuje voda ali odpadna voda. Ta metoda se uporablja za snovi, ki so dobro topne v vodi. Ta metoda se lahko s spremembami, navedenimi v standardih ISO 14442 in ISO 5667-16, uporabi za preskušanje zaviralnih učinkov slabo topnih in anorganskih snovi, hlapnih spojin, težkih kovin in odpadne vode. Hiter presejalni preskus zaviranja rasti alg v odpadni vodi je opisan v dodatku A. V dodatku B je opisan alternativni preskusni postopek z algami iz nizov, ki vključuje neposredno merjenje rasti alg v spektrofotometričnih celicah.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-junij-2012
1DGRPHãþD
SIST EN ISO 8692:2005
.DNRYRVWYRGH3UHVNXV]DYLUDQMDUDVWLVODGNRYRGQLKDOJ]HQRFHOLþQLPL]HOHQLPL
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Water quality - Freshwater algal growth inhibition test with unicellular green algae (ISO
8692:2012)
Wasserbeschaffenheit - Süßwasseralgen-Wachstumshemmtest mit einzelligen
Grünalgen (ISO 8692:2012)
Qualité de l'eau - Essai d'inhibition de la croissance des algues d'eau douce avec des
algues vertes unicellulaires (ISO 8692:2012)
Ta slovenski standard je istoveten z: EN ISO 8692:2012
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 8692
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2012
ICS 13.060.70 Supersedes EN ISO 8692:2004
English Version
Water quality - Fresh water algal growth inhibition test with
unicellular green algae (ISO 8692:2012)
Qualité de l'eau - Essai d'inhibition de la croissance des Wasserbeschaffenheit - Süßwasseralgen-
algues d'eau douce avec des algues vertes unicellulaires Wachstumshemmtest mit einzelligen Grünalgen (ISO
(ISO 8692:2012) 8692:2012)
This European Standard was approved by CEN on 14 February 2012.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2012 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 8692:2012: E
worldwide for CEN national Members.
Contents Page
Foreword .3
Foreword
This document (EN ISO 8692:2012) has been prepared by Technical Committee ISO/TC 147 “Water quality”
in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by
DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by August 2012, and conflicting national standards shall be withdrawn at
the latest by August 2012.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 8692:2004.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 8692:2012 has been approved by CEN as a EN ISO 8692:2012 without any modification.
INTERNATIONAL ISO
STANDARD 8692
Third edition
2012-02-15
Water quality — Fresh water algal growth
inhibition test with unicellular green algae
Qualité de l’eau — Essai d’inhibition de la croissance des algues d’eau
douce avec des algues vertes unicellulaires
Reference number
ISO 8692:2012(E)
©
ISO 2012
ISO 8692:2012(E)
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s
member body in the country of the requester.
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Published in Switzerland
ii © ISO 2012 – All rights reserved
ISO 8692:2012(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents and media . 2
6 Apparatus . 4
7 Procedure . 5
7.1 Preparation of growth medium . 5
7.2 Preparation of pre‑culture and inoculum . 5
7.3 Choice of test sample concentrations . 5
7.4 Preparation of test sample and stock solutions . 5
7.5 Preparation of test and control batches . 6
7.6 Incubation . 6
7.7 Measurements . 7
8 Validity criteria . 7
9 Calculation . 7
9.1 Plotting of growth curves . 7
9.2 Calculation of percentage inhibition . 8
9.3 Determination of E C (e.g. E C and E C ) . 8
r x r 10 r 50
10 Expression of results . 8
11 Interpretation of results . 9
12 Precision . 9
13 Test report . 9
Annex A (informative) Rapid screening of waste water algal growth inhibition . 11
Annex B (informative) Test procedure with algae from algal beads, with direct measurement of algal
growth in spectrophotometric cells .14
Annex C (informative) Procedure for immobilization of algae in alginate beads .19
Bibliography .21
ISO 8692:2012(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International
Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 8692 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods.
This third edition cancels and replaces the second edition (ISO 8692:2004), which has been technically revised.
iv © ISO 2012 – All rights reserved
INTERNATIONAL STANDARD ISO 8692:2012(E)
Water quality — Fresh water algal growth inhibition test with
unicellular green algae
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This International Standard does not purport to address all of the safety problems, if any,
associated with its use. It is the responsibility of the user to establish appropriate safety and health
practices and to ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this International
Standard be carried out by suitably qualified staff.
1 Scope
This International Standard specifies a method for the determination of the growth inhibition of unicellular
green algae by substances and mixtures contained in water or by waste water. This method is applicable for
substances that are easily soluble in water.
With modifications to this method, as specified in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly
soluble organic and inorganic materials, volatile compounds, heavy metals and waste water can be tested.
A rapid algal growth inhibition screening test for waste water is described in Annex A.
An alternative test procedure with algae from algal beads, with direct measurement of algal growth in
spectrophotometric cells, is described in Annex B.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are indispensable
for its application. For dated references, only the edition cited applies. For undated references, the latest edition
of the referenced document (including any amendments) applies.
ISO 5667-16, Water quality — Sampling — Part 16: Guidance on biotesting of samples
ISO/TR 11044, Water quality — Scientific and technical aspects of batch algae growth inhibition tests
ISO 14442, Water quality — Guidelines for algal growth inhibition tests with poorly soluble materials, volatile
compounds, metals and waste water
ISO/TS 20281, Water quality — Guidance on statistical interpretation of ecotoxicity data
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
cell density
n
number of cells per volume of medium
NOTE Cell density is expressed in cells per millilitre.
ISO 8692:2012(E)
3.2
effective concentration
concentration of the test sample (EC ) at which an effect of x % is measured, if compared to the control
x
NOTE To unambiguously denote an EC value deriving from growth rate, it is proposed to use the symbol “E C”.
r
3.3
l
...
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