Washing machines for household use - Method for measuring the microbiological performance

IEC TS 63429:2023 provides a test method for measuring the microbial contamination reduction performance of household washing machines with textile pieces contaminated with test microorganisms under standardized conditions. The microbial numbers on the contaminated textile pieces are measured before and after the washing programme and the reduction is calculated. Furthermore, a potential cross contamination from contaminated to uncontaminated textile pieces within the washing programme is measured.
This document does not address the microbial contamination reduction due to any potential or claimed antimicrobial action of detergents as well as of bleach systems or any additives.
This document applies to washing machines for household use, within the meaning that the scope of TC 59 indicates for household use, including the washing related functions of washer-dryers.
This document does not apply to professional washing machines nor to commercial laundry operations associated with food service, hospital linens or other non-residential applications.
This document deals with measurement procedures regarding the reduction of microbial contamination resulting from the use of electrical appliances for household and similar use. This document specifies methods that enable reproducible measurements. These derived measurement results can only be used for a relative statement. Absolute statements, i.e., health-related claims or conclusions about prevention or treatment of a disease or health improvement, are reserved for explicit regulatory action after a medical assessment.
This document does not apply to appliances intended to be used in medical, veterinary, or pharmaceutical applications.
This document does not address sanitization, disinfection, or sterilization measures.

General Information

Status
Published
Publication Date
18-Sep-2023
Current Stage
PPUB - Publication issued
Start Date
19-Jul-2023
Completion Date
19-Sep-2023
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Technical specification
IEC TS 63429:2023 - Washing machines for household use - Method for measuring the microbiological performance Released:19. 09. 2023
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IEC TS 63429 ®
Edition 1.0 2023-09
TECHNICAL
SPECIFICATION
Washing machines for household use – Method for measuring the
microbiological performance
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IEC TS 63429 ®
Edition 1.0 2023-09
TECHNICAL
SPECIFICATION
Washing machines for household use – Method for measuring the

microbiological performance
INTERNATIONAL
ELECTROTECHNICAL
COMMISSION
ICS 97.060  ISBN 978-2-8322-7477-4

– 2 – IEC TS 63429:2023 © IEC 2023
CONTENTS
FOREWORD . 4
INTRODUCTION . 6
1 Scope . 7
2 Normative references . 7
3 Terms, definitions and symbols. 8
3.1 Terms and definitions . 8
3.2 Symbols . 9
4 Requirements . 9
5 Test conditions, materials, equipment and instrumentation . 10
5.1 Test conditions . 10
5.2 Materials and reagents . 10
5.2.1 Microorganisms for test purposes . 10
5.2.2 Culture media and solutions . 11
5.2.3 Detergent . 13
5.3 Equipment . 14
5.3.1 General . 14
5.3.2 Incubator . 14
5.3.3 Autoclave . 14
5.3.4 Cotton carrier . 14
5.3.5 Biomonitor cloth . 14
5.3.6 Pipettes . 15
5.3.7 Electromechanical agitator. 15
5.3.8 Base load . 15
5.3.9 Measuring equipment for assessing temperature profile . 16
5.3.10 Measuring equipment for water consumption . 16
6 Tests . 16
6.1 Test method principles . 16
6.2 Preparation of washing machine . 16
6.3 Preparation of test microorganisms and biomonitors . 17
6.3.1 Cultures . 17
6.3.2 Biomonitors . 18
6.4 Main test . 19
6.4.1 General . 19
6.4.2 Evidence of test microorganisms . 19
6.5 Validation . 20
6.5.1 Enumeration of microorganisms before washing N (reference
biomonitor) . 20
6.5.2 Negative control (cross contamination) . 21
6.5.3 Determination of water quality . 21
6.5.4 Determination of water quantity in the main wash . 21
7 Evaluation . 21
7.1 Log reduction . 21
7.2 Cross contamination . 21
8 Test report . 22
Annex A (normative) Suitable methods to prepare the washing machine prior to test . 24
A.1 General . 24

A.2 Methods to prepare the washing machines before test . 24
A.2.1 Hot washing with the detergent . 24
A.2.2 Hot washing without detergent . 24
A.2.3 Treatment with sodium hypochlorite solution . 24
A.2.4 Treatment with peracetic acid solution (PAA) . 25
Annex B (informative) Sources of materials and supplies . 26
B.1 General . 26
B.2 Suppliers for test materials . 26
Bibliography . 27

Figure 1 – Biomonitor cloth . 15

Table 1 – Composition of TSA . 11
Table 2 – Composition of Sabouraud dextrose agar with chloramphenicol . 11
Table 3 – Composition of Tryptic Soy Broth (TSB) . 12
Table 4 – Composition of Baird-Parker agar . 12
Table 5 – Composition of cetrimide agar . 12
Table 6 – Composition of diluting agent . 13
Table 7 – Composition of neutralisation solution . 13
Table 8 – Temperature logger specification . 16

– 4 – IEC TS 63429:2023 © IEC 2023
INTERNATIONAL ELECTROTECHNICAL COMMISSION
____________
WASHING MACHINES FOR HOUSEHOLD USE –
METHOD FOR MEASURING THE MICROBIOLOGICAL PERFORMANCE

FOREWORD
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IEC TS 63429 has been prepared by subcommittee 59D: Performance of household and similar
electrical laundry appliances, of IEC Technical Committee 59: Performance of household and
similar electrical appliances. It is a Technical Specification.
The text of this Technical Specification is based on the following documents:
Draft Report on voting
59D/498/DTS 59D/503A/RVDTS
Full information on the voting for its approval can be fou
...

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