ISO 5553:2024

Meat and meat products — Detection of condensed phosphates

This document specifies a method for the detection of linear condensed phosphates in meat and meat products by thin layer chromatographic separation. This document only applies to the detection of added condensed phosphates that are still present in the sample at the time of investigation, because condensed phosphates are gradually hydrolyzed by enzymes present in meat or meat products and during heat treatment of meat or meat products.

Viandes et produits à base de viande — Recherche des phosphates condensés

Le présent document spécifie une méthode de recherche des phosphates condensés linéaires dans les viandes et les produits à base de viande, après séparation par chromatographie sur couche mince. Le présent document s’applique uniquement à la recherche des phosphates condensés ajoutés qui sont encore présents dans l’échantillon au moment de la recherche, étant donné que les phosphates condensés sont progressivement hydrolysés par les enzymes présentes dans les viandes ou les produits à base de viande et au cours du traitement par la chaleur des viandes ou des produits à base de viande.

General Information

• Status: Published
• Publication Date: 22-Feb-2024
• ICS: 67.120.10 - Meat and meat products
• Technical Committee: ISO/TC 34/SC 6 - Meat, poultry, fish, eggs and their products
• Drafting Committee: ISO/TC 34/SC 6 - Meat, poultry, fish, eggs and their products
• Current Stage: 6060 - International Standard published
• Start Date: 23-Feb-2024
• Due Date: 04-Jun-2024
• Completion Date: 23-Feb-2024

Relations

• Revises: ISO 5553:1980 - Meat and meat products — Detection of polyphosphates
• Effective Date: 06-Jun-2022

Standards Content (Sample)

International
Standard
ISO 5553
Second edition
Meat and meat products —
2024-02
Detection of condensed phosphates
Viandes et produits à base de viande — Recherche des phosphates
condensés
Reference number
ISO 5553:2024(en) © ISO 2024

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ISO 5553:2024(en)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland

© ISO 2024 – All rights reserved
ii

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ISO 5553:2024(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Sampling . 1
6 Preparation of the test sample. 1
7 Reagents and apparatus . 2
7.1 Reagents .2
7.2 Apparatus .3
8 Procedure . 4
8.1 Preparation of thin-layer plates .4
8.2 Preparation of serum .4
8.3 Chromatographic separation .4
8.4 Detection of phosphates .5
8.5 Limit of detection .5
8.6 Repeatability and reproducibility .5
9 Interpretation .
...

Norme
internationale
ISO 5553
Deuxième édition
Viandes et produits à base
2024-02
de viande — Recherche des
phosphates condensés
Meat and meat products — Detection of condensed phosphates
Numéro de référence
ISO 5553:2024(fr) © ISO 2024

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ISO 5553:2024(fr)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2024
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: [phone]
E-mail: [e-mail]
Web: www.iso.org
Publié en Suisse

© ISO 2024 – Tous droits réservés
ii

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ISO 5553:2024(fr)
Sommaire Page
Avant-propos .iv
1 Domaine d’application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe. 1
5 Échantillonnage . 1
6 Préparation de l’échantillon pour essai . 2
7 Réactifs et appareillage . 2
7.1 Réactifs .2
7.2 Appareillage .3
8 Mode opératoire . 4
8.1 Préparation des plaques à couche mince .4
8.2 Préparation du sérum .4
8.3 Séparation par chromatographie .4
8.4 Recherche des phosphates .5
8.5 Limite de détection .5
8.6 Répétabilité et reproductibilité .5
9 Interprétation .
...

FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 5553
ISO/TC 34/SC 6
Meat and meat products — Detection
Secretariat: SAC
of condensed phosphates
Voting begins on:
2023-11-21
Viandes et produits à base de viande — Recherche des phosphates
condensés
Voting terminates on:
2024-01-16
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/FDIS 5553:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023

---------------------- Page: 1 ----------------------
ISO/FDIS 5553:2023(E)
FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 5553
ISO/TC 34/SC 6
Meat and meat products — Detection
Secretariat: SAC
of condensed phosphates
Voting begins on:
Viandes et produits à base de viande — Recherche des phosphates
condensés
Voting terminates on:
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
CH-1214 Vernier, Geneva
DOCUMENTATION.
Phone: [phone]
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: [e-mail]
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/FDIS 5553:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN­
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
  © ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023

---------------------- Page: 2 ----------------------
ISO/FDIS 5553:2023(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Sampling . 1
6 Preparation of the test sample. 1
7 Reagents and apparatus . 2
7.1 Reagents . 2
7.2 Apparatus . 3
8 Procedure .4
8.1 Preparation of thin-layer plates .
...

ISO/DISFDIS 5553:2023(E)
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2023-07-2111-02
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ISO TC 34/SC 6/WG 23
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Secretariat: SAC
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Meat and meat products –Detection of condensed phosphates
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ISO/FDIS 5553:2023(E)
© ISO 20212023 Formatted: Section start: New page
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part
of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or
mechanical, including photocopying, or posting on the internet or an intranet, without prior written
permission. Permission can be requested from either ISO at the address below or ISO’s member body
in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.orgwww.iso.org
Published in Switzerland

ii © ISO 2023 – All rights reserved
ii © ISO 2023 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/FDIS 5553:2023(E)
Formatted: Right
Contents
Foreword . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Sampling . 1
6 Preparation of the test sample . 1
7 Reagents and apparatus . 2
7.1 Reagents . 2
7.2 Apparatus . 3
8 Procedure . 4
8.1 Preparation of thin-layer plates . 4
8.2 Preparation of serum .
...