ISO/TC 34/SC 6 - Meat, poultry, fish, eggs and their products

This document defines terms for meat and meat products. It is applicable to the processing, trade and storage of meat and meat products.

This document specifies the requirements for frozen surimi and the test methods for its quality control. It also specifies the requirements of packaging, marking, storage and transportation. This document is applicable to tropical and cold-water surimi.

Le présent document spécifie les exigences de production et sanitaires applicables aux produits fermentés à base de viande et établit une série de méthodes d’essai destinées à maîtriser la qualité des produits fermentés à base de viande. Il spécifie également les exigences de transport, de stockage, d’emballage et d’étiquetage. Le présent document est applicable aux produits fermentés à base de viande (du type prêt à consommer), notamment la saucisse fermentée, le jambon sec fermenté et d’autres produits fermentés à base de viande. Il est également applicable à la production de viande fermentée et aux relations commerciales.

This document specifies pre-slaughter requirements, operating procedures and requirements of pig slaughtering, storage and other requirements. This document is applicable to the slaughtering operation of pigs. For some categories of pigs (e.g. piglets, breeding pigs), other operating procedures can be applied.

This document specifies the liquid chromatographic (LC) method for the determination of chloramphenicol content of muscle tissue of meat, including livestock and poultry. This document specifies the liquid chromatography tandem mass spectrometry method (LC-MS/MS) for the determination of chloramphenicol content of muscle tissue, casing, liver of meat and meat products, including livestock and poultry. This document specifies LC-MS/MS as the reference method. The LC method is suitable for the determination of chloramphenicol content greater than 6,5 mg/kg. LC-MS/MS is suitable for the determination of chloramphenicol content greater than 0,1 μg/kg. Test samples which have deteriorated cannot be analysed with this method.

This document specifies a detection method using thin-layer chromatography and a determination method using high performance liquid chromatography (HPLC) for synthetic colouring agents in meat and meat products. This document specifies the HPLC method as the reference method. This document is applicable to meat and meat products, including livestock and poultry products. The method using thin-layer chromatography can detect the following colouring agents: Tartrazine, Patent Blue V, Quinoline Yellow, Indigotine, Sunset Yellow FCF, Brilliant Black PN, Amaranth, Black 7984, Ponceau 4R, Fast Green FCF, Erythrosine, Blue VRS. Synonyms and identity numbers of these colouring agents are listed in Annex A. The plant colours and plant extracts which have been observed not to interfere with this method are listed in B.1. Natural colours which in some cases have been shown to interfere with this method are listed in B.2. The method using HPLC can detect the following colouring agents: Tartrazine, Allura Red AC, Amaranth, Brilliant Blue FCF, Ponceau 4R, New Red, Sunset Yellow FCF, Carmoisine, Erythrosine, Indigotine. Chromatograms of these standard reference colours are shown in Annex D.

This document specifies three methods for the determination of the total phosphorous content of all kinds of meat and meat products, including poultry and livestock: the inductively coupled plasma optical emission spectrometry (ICP-OES) method; the spectrometric method; the gravimetric method. For the ICP-OES method, the limit of detection (LOD) is 1,0 mg/kg and the limit of quantification (LOQ) is 3,0 mg/kg if the mass of the test portion is 0,5 g and the constant volume is 50 ml.

This document specifies the spectrophotometer method and the light absorption microplate reader method for the determination of the free L-(+)-glutamic acid content of meat and meat products. This document is applicable to meat and meat products, including livestock and poultry products.

This International Standard specifies the reference method for measuring the pH of all kinds of meat and meat products, including poultry. The method is applicable to products which may be homogenized and also to non-destructive measurements on carcass meat, quarters and muscles.

Describes a reference method for the determination of the moisture content of meat and meat products. Replaces the first edition.

Gives a method for the determination of the chloride content of meat and meat products, including poultry, with sodium chloride contents equal to or greater than 0,25 % (m/m).

Gives a method for the determination of the chloride content of meat and meat products, including poultry, with sodium chloride contents equal to or greater than 1,0 % (m/m).

Specifies a method for the determination of the free fat content of meat and meat products by means of extraction.

Cancels and replaces the first edition (1978). Specifies a method for the determination of the hydroxyproline content of all kinds of meat and meat products, including poultry. Applicable to meat and meat products containing less than 0,5 % (m/m) hydroxyproline.

The method consists in digesting of a test portion with concentrated sulphuric acid, using copper(II) sulphate as a catalyst, to convert organic nitrogen to ammonia ions. Then alkalisation, distillation of the liberated ammonia into an excess of boric acid solution, titration with hydrochloric acid to determine the ammonia bound by the boric acid, and calculation of nitrogen content of the sample from the amount of ammonia produced.

The method consists in heating a test portion with ethanolic potassium hydroxide solution until the meat components are totally dissolved, decanting, washing of the remaining residue with hot ethanol, filtering, dissolving in hydrochloric acid, and hydrolysis, followed by titrimetric determination of the glucose formed.

The method consists in extracting of a test portion with hot water, precipitating of proteine and filtrating. In presence of nitrits formation of a red cloured complex by adding sulfanilamide and naphthylethylenediamine hydrochloride, and photometric measurement at a wavelength of 538 nm.

The method consists in extracting of a test portion with hot water, precipitating of proteins and filtrating. Reduction of the extracted nitrates into nitrites by metallic cadmium. Formation of a red colouration by adding of sulfanilamide and nphthyl ethylenediamine hydrochloride and photometric measurement at a wavelength of 538 nm.

The method consists in boiling of a test portion with hydrochloric acid to free the occluded and bound lipid fractions, filtrating of the resulting mass, drying, and extracting with n-hexane or light petroleum, of the fat retained on the filter.

This International Standard specifies a thin-layer chromatographic method for the detection of synthetic, watersoluble colouring agents in meat and meat products. The following colouring agents can be detected with the method: Tartrazine Patent Blue V Quinoline Yellow Indigotine Sunset Yellow FCF Brilliant Black PN Amaranth Black 7984 Ponceau 4R Fast Green FCF Erythrosine Blue VRS Synonyms and identity numbers of these colouring agents are listed in annex A. The plant colours and plant extracts which have been observed not to interfere with this method are listed in Table B.1. Natural colours which in some cases have been shown to interfere with this method are listed in Table B.2.

This International Standard specifies a reference method for the determination of the L-(+)-glutamic acid content of meat and meat products, including poultry.

Contains a spectrometric method for the determination of the total phosphorus content of meat and meat products, including poultry.

Describes a method for the enumeration of Pseudomonas spp. in meat and meat products, including poultry, by means of a colony-count technique after aerobic incubation at 25 °C for 48 h.

This second edition cancels and replaces the first edition (ISO 3100-1:1975). Part 1 of ISO 3100 deals with taking primary samples or secondary samples from meat and meat products. It applies to sampling for commercial purposes. In the case of official food inspection, it may be necessary to follow other procedures. Gives a normative reference. The definitions given in ISO 7002 apply. Specifies sampling equipment, number of sampling units to be taken, sampling procedure and sampling report, packaging,transport and storage of sampling units.

The method gives general instructions and specifies procedures to be followed after taking a laboratory sample. This may require the thawing and/or mincing of "open" samples or the pre-incubation, external sterilisation, and aseptic opening of products processed or packed in sealed units.

The method consists in extracting of the compound with ice-cold perchloric acid solution, centrifuging, decanting and filtrating, followed by hydrolysis of the compound in a portion of the filtrate with potassium hydroxide into gluconate. Transformation of the gluconate in the extract by the following reactions: 1) with adenosine-5-triphosphate (ATP) and 2) with concomitant reduction of an equivalent amount of nicotinamide adenine dinucleotide phosphate (NADP). Photometric measurement of the amount of nicotinamide adenine dinucleotide phosphate (reduced) (NADPH) formed.

The method consists in mineralizing of a test portion with sulphuric and nitrit acids, precipitating of the phosphorus as quinoline phosphomolybdate, drying and weighing of the precipitate. An alternative method of mineralizing is described in clause 10.