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This International Standard specifies a thin-layer chromatographic method for the detection of synthetic, watersoluble colouring agents in meat and meat products. The following colouring agents can be detected with the method: Tartrazine Patent Blue V Quinoline Yellow Indigotine Sunset Yellow FCF Brilliant Black PN Amaranth Black 7984 Ponceau 4R Fast Green FCF Erythrosine Blue VRS Synonyms and identity numbers of these colouring agents are listed in annex A. The plant colours and plant extracts which have been observed not to interfere with this method are listed in Table B.1. Natural colours which in some cases have been shown to interfere with this method are listed in Table B.2.

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This International Standard specifies the reference method for measuring the pH of all kinds of meat and meat products, including poultry. The method is applicable to products which may be homogenized and also to non-destructive measurements on carcass meat, quarters and muscles.

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This International Standard specifies a reference method for the determination of the L-(+)-glutamic acid content of meat and meat products, including poultry.

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Describes a reference method for the determination of the moisture content of meat and meat products. Replaces the first edition.

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Contains a spectrometric method for the determination of the total phosphorus content of meat and meat products, including poultry.

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Gives a method for the determination of the chloride content of meat and meat products, including poultry, with sodium chloride contents equal to or greater than 1,0 % (m/m).

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Gives a method for the determination of the chloride content of meat and meat products, including poultry, with sodium chloride contents equal to or greater than 0,25 % (m/m).

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Specifies a method for the determination of the free fat content of meat and meat products by means of extraction.

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Cancels and replaces the first edition (1978). Specifies a method for the determination of the hydroxyproline content of all kinds of meat and meat products, including poultry. Applicable to meat and meat products containing less than 0,5 % (m/m) hydroxyproline.

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The method consists in digesting of a test portion with concentrated sulphuric acid, using copper(II) sulphate as a catalyst, to convert organic nitrogen to ammonia ions. Then alkalisation, distillation of the liberated ammonia into an excess of boric acid solution, titration with hydrochloric acid to determine the ammonia bound by the boric acid, and calculation of nitrogen content of the sample from the amount of ammonia produced.

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The method consists in heating a test portion with ethanolic potassium hydroxide solution until the meat components are totally dissolved, decanting, washing of the remaining residue with hot ethanol, filtering, dissolving in hydrochloric acid, and hydrolysis, followed by titrimetric determination of the glucose formed.

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The method consists in extracting of a test portion with hot water, precipitating of proteins and filtrating. Reduction of the extracted nitrates into nitrites by metallic cadmium. Formation of a red colouration by adding of sulfanilamide and nphthyl ethylenediamine hydrochloride and photometric measurement at a wavelength of 538 nm.

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The method consists in extracting of a test portion with hot water, precipitating of proteine and filtrating. In presence of nitrits formation of a red cloured complex by adding sulfanilamide and naphthylethylenediamine hydrochloride, and photometric measurement at a wavelength of 538 nm.

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The method consists in mineralizing of a test portion with sulphuric and nitrit acids, precipitating of the phosphorus as quinoline phosphomolybdate, drying and weighing of the precipitate. An alternative method of mineralizing is described in clause 10.

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The method consists in boiling of a test portion with hydrochloric acid to free the occluded and bound lipid fractions, filtrating of the resulting mass, drying, and extracting with n-hexane or light petroleum, of the fat retained on the filter.

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Describes a method for the enumeration of Pseudomonas spp. in meat and meat products, including poultry, by means of a colony-count technique after aerobic incubation at 25 °C for 48 h.

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This second edition cancels and replaces the first edition (ISO 3100-1:1975). Part 1 of ISO 3100 deals with taking primary samples or secondary samples from meat and meat products. It applies to sampling for commercial purposes. In the case of official food inspection, it may be necessary to follow other procedures. Gives a normative reference. The definitions given in ISO 7002 apply. Specifies sampling equipment, number of sampling units to be taken, sampling procedure and sampling report, packaging,transport and storage of sampling units.

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The method gives general instructions and specifies procedures to be followed after taking a laboratory sample. This may require the thawing and/or mincing of "open" samples or the pre-incubation, external sterilisation, and aseptic opening of products processed or packed in sealed units.

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The method consists in extracting of the compound with ice-cold perchloric acid solution, centrifuging, decanting and filtrating, followed by hydrolysis of the compound in a portion of the filtrate with potassium hydroxide into gluconate. Transformation of the gluconate in the extract by the following reactions: 1) with adenosine-5-triphosphate (ATP) and 2) with concomitant reduction of an equivalent amount of nicotinamide adenine dinucleotide phosphate (NADP). Photometric measurement of the amount of nicotinamide adenine dinucleotide phosphate (reduced) (NADPH) formed.

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