ISO 7581

Evaluation of bactericidal activity of a non-porous antimicrobial surface used in a dry environment

This document specifies the test conditions and the levels of activity to determine the bactericidal activity of non-porous surfaces used in a dry environment . It defines a protocol to validate the bactericidal character of a surface and to measure its performance. It is not intended to be used to substantiate cleaning or disinfecting properties. This document is applicable to surfaces claiming to have an activity against vegetative bacteria. The olbigatory test conditions are defined in this document. It does not apply to porous surfaces. It does not refer to methods for testing the toxicological and ecotoxicological properties of the surfaces. This document is used to measure bactericidal action, not bacteriostatic activity of a surface.

Évaluation de l’activité bactéricide d’une surface antimicrobienne non poreuse utilisée dans un environnement sec

General Information

• Status: Not Published
• ICS: 07.100.99 - Other standards related to microbiology
• Technical Committee: ISO/TC 330 - Surfaces with biocidal and antimicrobial properties
• Drafting Committee: ISO/TC 330 - Surfaces with biocidal and antimicrobial properties
• Current Stage: 6000 - International Standard under publication
• Completion Date: 31-Oct-2023

Standards Content (Sample)

FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 7581
ISO/TC 330
Evaluation of bactericidal activity of
Secretariat: AFNOR
a non-porous antimicrobial surface
Voting begins on:
2023-09-04 used in a dry environment
Voting terminates on:
2023-10-30
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/FDIS 7581:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023

---------------------- Page: 1 ----------------------
ISO/FDIS 7581:2023(E)
FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 7581
ISO/TC 330
Evaluation of bactericidal activity of
Secretariat: AFNOR
a non-porous antimicrobial surface
Voting begins on:
used in a dry environment
Voting terminates on:
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
CH-1214 Vernier, Geneva
DOCUMENTATION.
Phone: [phone]
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: [e-mail]
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/FDIS 7581:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN­
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
  © ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023

---------------------- Page: 2 ----------------------
ISO/FDIS 7581:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative reference .1
3 Terms and definitions . 1
4 Apparatus, reagents and materials . 3
4.1 Test organisms . 3
4.2 Culture media and reagents. 3
4.3 Reference and test surfaces . 4
4.3.1 General . 4
4.3.2 Reference surfaces . 4
4.3.3 Test surfaces . . 5
4.4 Apparatus and materials .
...

Pr ISO /FDIS 7581:2022(E)
ISO /TC 330
Secretariat: AFNOR
Date: 2022-06-232023-08-18
Evaluation of bactericidal activity of a non-porous antimicrobial
surface used in a dry environment
FDIS stage

---------------------- Page: 1 ----------------------
ISO/DIS 7581:2022(E)
© ISO 20222023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
EmailE-mail: [e-mail]
Website: www.iso.orgwww.iso.org
Published in Switzerland
ii © ISO 2022 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/DIS 7581:2022(E)
Contents
Foreword . 5
Introduction . 6
1 Scope . 1
2 Normative reference . 1
3 Terms and definitions . 1
4 Apparatus, reagents and materials . 3
4.1 Test organisms . 3
4.2 Culture media and reagents . 3
4.3 Reference and test surfaces . 5
4.3.1 General . 5
4.3.2 Reference surfaces . 5
4.3.3 Test surfaces . 5
4.4 Apparatus and materials . 6
5 Preparation of the test organism suspensions . 8
5.1 Stock cultures of the test bacteria . 8
5.2 Working culture of the test bacteria . 8
5.3 Test suspensions . 8
5.4 Quantifying of bacterial test suspensions . 8
5.5 Calculation of the weighted mean bacteria concentration in the test suspensions . 9
6 Evaluation method of the bactericidal activity of a non-porous surface and its efficacy . 10
6.1 Obligatory experimental conditions . 10
6.2 Test procedure . 10
6.2.1 Sampling . 10
6.2.2 Test (T0 and Txh) . 11
6.2.3 Reference surfaces (C and C ) . 12
0 xh
6.2.4 Validation (
...