Microscopes — Cover glasses — Part 1: Dimensional tolerances, thickness and optical properties

ISO 8255-1:2017 specifies requirements for dimensional tolerances, thickness and optical properties for microscope cover glasses used for transmitted light microscopy in the visible spectral range (400 nm to 760 nm).

Microscopes — Lamelles couvre-objet — Partie 1: Tolérances dimensionnelles, épaisseur et propriétés optiques

General Information

Publication Date
Current Stage
9093 - International Standard confirmed
Completion Date
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ISO 8255-1:2017 - Microscopes -- Cover glasses
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Standards Content (Sample)

Third edition
Microscopes — Cover glasses —
Part 1:
Dimensional tolerances, thickness and
optical properties
Microscopes — Lamelles couvre-objet —
Partie 1: Tolérances dimensionnelles, épaisseur et propriétés optiques
Reference number
ISO 8255-1:2017(E)
ISO 2017

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ISO 8255-1:2017(E)

© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
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ISO copyright office
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Tel. +41 22 749 01 11
Fax +41 22 749 09 47
ii © ISO 2017 – All rights reserved

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ISO 8255-1:2017(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Requirements . 1
4.1 General . 1
4.2 Dimensional tolerances for thickness . 1
4.3 Dimensional tolerances for length, width and diameter . 2
4.4 Optical properties . 2
5 Marking and labelling . 3
Annex A (informative) Guidelines for selection of cover glass type . 4
Annex B (informative) Typical dimensions for rectangular and round cover glasses .5
Bibliography . 6
© ISO 2017 – All rights reserved iii

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ISO 8255-1:2017(E)

ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
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For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: w w w . i s o .org/ iso/ foreword .html.
The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 5,
Microscopes and endoscopes.
This third edition cancels and replaces the second edition (ISO 8255-1:2011), of which it constitutes a
minor revision to correct the designation of cover glass.
A list of all parts in the ISO 8255 series can be found on the ISO website.
iv © ISO 2017 – All rights reserved

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ISO 8255-1:2017(E)

This document defines dimensions and specifies optical quality requirements in order to guarantee the
quality of observation.
The data given in this document are applicable to most products in use and have been adapted to take
into account the relevant national standards in vigour.
This document contains requirements for dimensional tolerances, thickness and optical properties,
whereas quality requirements and test methods related to the material a

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