ISO/TS 22082:2020

Nanotechnologies — Assessment of nanomaterial toxicity using dechorionated zebrafish embryo

This document specifies a method for rapidly assessing nanomaterial toxicity (fish early life stage, 0 HPF to 120 HPF). It includes information on the importance of acellular chorion removal, detailed chorion removal procedures, and a complete protocol for the toxicity assessment of nanomaterials using dechorionated zebrafish embryos. The focus of this document is on testing nanomaterial toxicity.

Nanotechnologies — Évaluation de la toxicité des nanomatériaux au moyen d'embryons déchorionés de poisson zèbre

General Information

• Status: Published
• Publication Date: 05-May-2020
• ICS: 07.120 - Nanotechnologies
• Technical Committee: ISO/TC 229 - Nanotechnologies
• Drafting Committee: ISO/TC 229/WG 3 - Health, Safety and Environmental Aspects of Nanotechnologies
• Current Stage: 9093 - International Standard confirmed
• Completion Date: 04-Oct-2023

Standards Content (Sample)

TECHNICAL ISO/TS
SPECIFICATION 22082
First edition
2020-05
Nanotechnologies — Assessment
of nanomaterial toxicity using
dechorionated zebrafish embryo
Nanotechnologies — Évaluation de la toxicité des nanomatériaux au
moyen d'embryons déchorionés de poisson zèbre
Reference number
ISO/TS 22082:2020(E)
©
ISO 2020

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ISO/TS 22082:2020(E)

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ii © ISO 2020 – All rights reserved

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ISO/TS 22082:2020(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 2
5 Materials . 2
6 Apparatus . 3
6.1 Technical equipment . 3
6.2 Analytical instruments . 3
7 Procedures . 4
7.1 Culture . 4
7.1.1 Zebrafish strain . 4
7.1.2 Feeding regime . 4
7.1.3 Photoperiod . 4
7.1.4 Temperature and pH . . 4
7.1.5 Tank material . 4
7.2 Spawning stimulation . 4
7.3 Dechorionation of e
...