ISO 6870:2002

Animal feeding stuffs — Qualitative determination of zearalenone

This International Standard specifies a qualitative method for the determination of zearalenone in animal feeding stuffs and, in particular, in maize. This method is for screening purposes only. The limit of determination of zearalenone is approximately 50 μg/kg . NOTE Although sorghum gives interfering fluorescent spots identical to those of zearalenone, the method is still applicable to this feed because Rf the values are different after development of the chromatogram in the second direction. These spots are not developed by the specified confirmation technique.

Aliments des animaux — Dosage qualitatif de la zéaralénone

La présente Norme internationale spécifie une méthode qualitative de dosage de la zéaralénone dans les aliments des animaux et en particulier dans le maïs. Cette méthode ne sert qu'à des fins de détection. La limite de détermination de la zéaralénone se situe aux environs de 50 μg/kg . NOTE Bien que le sorgho présente des taches parasites de fluorescence identiques à celles de la zéaralénone, la méthode est applicable à cet aliment, car les valeurs de Rf sont différentes après le développement du chromatogramme dans la seconde direction. Ces taches ne sont pas révélées par la technique de confirmation spécifiée.

Krma - Kvalitativno določevanje zearalenona

General Information

• Status: Published
• Publication Date: 29-May-2002
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: ISO/TC 34/SC 10 - Animal feeding stuffs
• Drafting Committee: ISO/TC 34/SC 10 - Animal feeding stuffs
• Current Stage: 9060 - Close of review
• Start Date: 02-Sep-2029
• Ref Project: SIST ISO 6870:2002 - Animal feeding stuffs -- Qualitative determination of zearalenone

Relations

• Revised: SIST ISO 6870:1995 - Animal feeding stuffs -- Determination of zearalenone content
• Effective Date: 12-May-2008
• Revises: ISO 6870:1985 - Animal feeding stuffs — Determination of zearalenone content
• Effective Date: 15-Apr-2008

Standards Content (Sample)

SLOVENSKI STANDARD
SIST ISO 6870:2002
01-november-2002
.UPD.YDOLWDWLYQRGRORþHYDQMH]HDUDOHQRQD
Animal feeding stuffs -- Qualitative determination of zearalenone
Aliments des animaux -- Dosage qualitatif de la zéaralénone
Ta slovenski standard je istoveten z: ISO 6870:2002
ICS:
65.120 Krmila Animal feeding stuffs
SIST ISO 6870:2002 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST ISO 6870:2002

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SIST ISO 6870:2002
INTERNATIONAL ISO
STANDARD 6870
Second edition
2002-04-15
Animal feeding stuffs — Qualitative
determination of zearalenone
Aliments des animaux — Dosage qualitatif de la zéaralénone

Reference number
ISO 6870:2002(E)
©
ISO 2002

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SIST ISO 6870:2002
ISO 6870:2002(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be
edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file,
parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters
were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event
that a problem relating to it is found, please inform the Central Secretariat at the address given below.
© ISO 2002
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's
member body in the country of the requester.
ISO copyright office
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Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
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Printed in Switzerland
©
ii ISO 2002 – All rights reserved

---------------------- Page: 4 ----------------------

SIST ISO 6870:2002
ISO 6870:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 6870 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 10, Animal feeding stuffs.
This second edition cancels and replaces the first edition (ISO 6870:1985), of which it constitutes a minor revision.
The title has been changed to stress that the method is only qualitative and the scope now states that the method is
for screening purposes only. The temperature range in 3.8.2 has been corrected to 0 °C to 5 °C.
Annex A of this International Standard is for information only.
©
ISO 2002 – All rights reserved iii

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SIST ISO 6870:2002
INTERNATIONAL STANDARD ISO 6870:2002(E)
Animal feeding stuffs — Qualitative determination of zearalenone
1 Scope
This International Standard specifies a qualitative method for the determination of zearalenone in animal feeding
stuffs and, in particular, in maize. This method is for screening purposes only.
The limit of determination of zearalenone is approximately 50µg/kg.
NOTE Although sorghum gives interfering fluorescent spots identical to those of zearalenone, the method is still applicable to this
feed because the R values are different after development of the chromatogram in the second direction. These spots are not
f
developed by the specified confirmation technique.
2 Princi
...

INTERNATIONAL ISO
STANDARD 6870
Second edition
2002-04-15
Animal feeding stuffs — Qualitative
determination of zearalenone
Aliments des animaux — Dosage qualitatif de la zéaralénone

Reference number
ISO 6870:2002(E)
©
ISO 2002

---------------------- Page: 1 ----------------------
ISO 6870:2002(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be
edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file,
parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters
were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event
that a problem relating to it is found, please inform the Central Secretariat at the address given below.
© ISO 2002
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's
member body in the country of the requester.
ISO copyright office
Case postale 56  CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.ch
Printed in Switzerland
©
ii ISO 2002 – All rights reserved

---------------------- Page: 2 ----------------------
ISO 6870:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 6870 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 10, Animal feeding stuffs.
This second edition cancels and replaces the first edition (ISO 6870:1985), of which it constitutes a minor revision.
The title has been changed to stress that the method is only qualitative and the scope now states that the method is
for screening purposes only. The temperature range in 3.8.2 has been corrected to 0 °C to 5 °C.
Annex A of this International Standard is for information only.
©
ISO 2002 – All rights reserved iii

---------------------- Page: 3 ----------------------
INTERNATIONAL STANDARD ISO 6870:2002(E)
Animal feeding stuffs — Qualitative determination of zearalenone
1 Scope
This International Standard specifies a qualitative method for the determination of zearalenone in animal feeding
stuffs and, in particular, in maize. This method is for screening purposes only.
The limit of determination of zearalenone is approximately 50µg/kg.
NOTE Although sorghum gives interfering fluorescent spots identical to those of zearalenone, the method is still applicable to this
feed because the R values are different after development of the chromatogram in the second direction. These spots are not
f
developed by the specified confirmation technique.
2 Principle
A test portion is extracted with a mixture of acetonitrile and potassium chloride solution, then filtered, and an aliquot
portion is defatted with isooctane, followed by purification in a mixture of acetonitrile, water and lead acetate in the
presence of diatomaceous earth. After filtration, an aliquot portion is extracted with chloroform which is subsequently
evaporated.
The dry extract is dissolved in a mixture of benzene and acetonitrile. Two-dimensional thin-layer chromatography is
performed on an aliquot portion of this solution. The zearalenone content is determined by visual measurement or by
measurement of the intensity of fluorescence of the spot u
...

NORME ISO
INTERNATIONALE 6870
Deuxième édition
2002-04-15
Aliments des animaux — Dosage qualitatif
de la zéaralénone
Animal feeding stuffs — Qualitative determination of zearalenone

Numéro de référence
ISO 6870:2002(F)
©
ISO 2002

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ISO 6870:2002(F)
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Imprimé en Suisse
©
ii ISO 2002 – Tous droits réservés

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ISO 6870:2002(F)
Avant-propos
L'ISO (Organisation internationale de normalisation) est une fédération mondiale d'organismes nationaux de
normalisation (comités membres de l'ISO). L'élaboration des Normes internationales est en général confiée aux
comités techniques de l'ISO. Chaque comité membre intéressé par une étude a le droit de faire partie du comité
technique créé à cet effet. Les organisations internationales, gouvernementales et non gouvernementales, en liaison
avec l'ISO participent également aux travaux. L'ISO collabore étroitement avec la Commission électrotechnique
internationale (CEI) en ce qui concerne la normalisation électrotechnique.
Les Normes internationales sont rédigées conformément aux règles données dans les Directives ISO/CEI, Partie 3.
Les projets de Normes internationales adoptés par les comités techniques sont soumis aux comités membres pour
vote. Leur publication comme Normes internationales requiert l'approbation de 75 % au moins des comités membres
votants.
L'attention est appelée sur le fait que certains des éléments de la présente Norme internationale peuvent faire l'objet
de droits de propriété intellectuelle ou de droits analogues. L'ISO ne saurait être tenue pour responsable de ne pas
avoir identifié de tels droits de propriété et averti de leur existence.
La Norme internationale ISO 6870 a été élaborée par le comité technique ISO/TC 34, Produits alimentaires, sous-
comité SC 10, Aliments des animaux.
Cette deuxième édition annule et remplace la première édition (ISO 6870:1985), dont elle constitue une révision
mineure. Le titre a été modifé pour souligner le fait que la méthode n’est que qualitative, et dans le domaine
d’application il est maintenant spécifié que la méthode ne sert qu’à des fins de détection. La plage des températures
indiquée en 3.8.2 a été rectifiée pour lire 0 °C à 5 °C.
L'annexe A de la présente Norme internationale est donnée uniquement à titre d'information.
©
ISO 2002 – Tous droits réservés iii

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NORME INTERNATIONALE ISO 6870:2002(F)
Aliments des animaux — Dosage qualitatif de la zéaralénone
1 Domaine d'application
La présente Norme internationale spécifie une méthode qualitative de dosage de la zéaralénone dans les aliments
des animaux et en particulier dans le maïs. Cette méthode ne sert qu'à des fins de détection.
La limite de détermination de la zéaralénone se situe aux environs de 50µg/kg.
NOTE Bien que le sorgho présente des taches parasites de fluorescence identiques à celles de la zéaralénone, la méthode est
applicable à cet aliment, car les valeurs de R sont différentes après le développement du chromatogramme dans la seconde
f
direction. Ces taches ne sont pas révélées par la technique de confirmation spécifiée.
2Principe
Extraction sur une prise d'essai par un mélange d'acétonitrile et de solution de chlorure de potassium. F
...