SIST EN 15458:2022

Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae

This document specifies a laboratory test method for determining the biocidal or biostatic efficacy of single active substances or combinations thereof used in film preservatives of coatings against algal growth. The document does not apply to coatings unsusceptible to algal growth. The test method covers only active substances for film preservation, not the substrate itself, e.g. wood, which is dealt with in another standard. The test method is applicable for active substances used for wood protection and masonry coatings. It is not applicable to marine coatings.
Safety, health and environmental aspects are not in the scope of this document.
Determination of the performance of film preservatives in coatings by applying ageing procedures is not within the scope of this document.

Beschichtungsstoffe - Laborverfahren für die Prüfung der Wirksamkeit von Filmkonservierungsmitteln in einer Beschichtung gegen Algen

Dieses Dokument legt ein Laborverfahren zum Bestimmen der bioziden/biostatischen Wirksamkeit von Wirkstoffen oder einer Kombination aus mehreren Wirkstoffen zur Anwendung in Filmkonservierungsmitteln in Beschichtungen gegen Algenbewuchs fest. Dieses Dokument gilt nicht für Beschichtungen, die unempfindlich gegen Algenbewuchs sind. Das Prüfverfahren deckt nur die Wirkstoffe zur Filmkonservierung der Beschichtung ab, nicht zum Schutz des Substrats, z. B. Holz. Dieser wird in einer anderen Norm behandelt. Das Prüfverfahren ist anwendbar für Wirkstoffe, die in Beschichtungen für den Schutz von Holz und für mineralische Substrate verwendet werden. Die Norm gilt nicht für Beschichtungen im maritimen Bereich.
Sicherheits-, Gesundheits- und Umweltaspekte liegen nicht im Anwendungsbereich dieses Dokuments.
Die Bestimmung der Wirksamkeit von Filmkonservierungsmitteln in Beschichtungen nach Anwendung von Alterungsverfahren liegt nicht im Anwendungsbereich dieses Dokuments.

Peintures et vernis - Méthode d'essai en laboratoire permettant de déterminer l'efficacité des préservateurs du feuil d'un revêtement contre les algues

Le présent document spécifie une méthode d’essai en laboratoire permettant de déterminer l’efficacité biocide ou biostatique de substances actives individuelles, ou de combinaisons de ces substances, utilisées dans les préservateurs du feuil de revêtements contre la prolifération d’algues. Ce document ne s’applique pas aux revêtements qui ne sont pas sensibles à la prolifération d’algues. Cette méthode d’essai concerne uniquement les substances actives utilisées pour la protection du feuil et non du subjectile lui même, par exemple le bois, laquelle fait l’objet d’une autre norme. Cette méthode d’essai est applicable aux substances actives utilisées pour la protection des peintures pour bois et pour maçonnerie. Elle ne s’applique pas aux peintures marines.
Les aspects de sécurité et de santé, ainsi que les aspects environnementaux, ne relèvent pas du domaine d’application du présent document.
La détermination des performances des produits de préservation du feuil de revêtements par des méthodes de vieillissement n’est pas traitée dans le présent document.

Barve in laki - Laboratorijska metoda za preskušanje učinkovitosti konzervansov filma v premazih proti algam

Ta evropski standard določa laboratorijsko preskusno metodo za določanje biocidne/biostatične učinkovitosti posamezne aktivne snovi ali kombinacij aktivnih snovi, ki se uporabljajo v konzervansih filma v premazih proti algam. Standard se ne uporablja za premaze, ki niso občutljivi na alge. Preskusna metoda vključuje samo aktivne snovi za ohranjanje filma in ne za zaščito same podlage, npr. lesa, ki jo obravnava drug standard. Preskusna metoda se uporablja za aktivne snovi, ki se uporabljajo za lesne in zidne premaze. Ne uporablja se za ladijske premaze.
Na področje uporabe tega standarda niso vključeni vidiki varnosti, zdravja in varstva okolja.
Določanje učinkovitosti konzervansov filma v premazih z izvajanjem postopkov staranja ne spada na področje uporabe tega standarda.

General Information

• Status: Published
• Public Enquiry End Date: 30-Jun-2020
• Publication Date: 17-Aug-2022
• ICS: 87.040 - Paints and varnishes
• Technical Committee: IBLP - Paints and varnishes
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 01-Aug-2022
• Due Date: 06-Oct-2022
• Completion Date: 18-Aug-2022
• Ref Project: EN 15458:2022 - Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae

Relations

• Replaces: SIST EN 15458:2014 - Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae
• Effective Date: 01-Sep-2022

Overview - EN 15458:2022 (Paints and varnishes)

EN 15458:2022 specifies a laboratory method to determine the biocidal or biostatic efficacy of film preservatives in coatings against algae. It is a semiquantitative, comparative test between coatings with and without film preservatives and is intended for film preservation (not for preservative treatment of substrates). The method supports evaluation of active substances for regulatory assessment (e.g., inclusion considerations under the EU Biocidal Product Regulation, BPR 528/2012). The standard supersedes EN 15458:2014 and applies to coatings susceptible to algal growth such as wood protection and masonry coatings. It is not applicable to marine coatings. Safety, environmental aspects and accelerated ageing procedures are explicitly out of scope.

Key topics and technical requirements

• Test principle: coated filter-paper specimens are conditioned, placed onto agar medium, inoculated with a standard algal suspension and incubated; algal growth intensity on the coated surface is assessed.
• Microorganisms: selects one blue‑green and one green alga from defined strains (examples include Nostoc commune, Gloeocapsa atrata, Klebsormidium flaccidum, Stichococcus bacillaris).
• Culture medium: Bold’s Basal Medium (solid with ~1.5% agar for plates; recipe and stock solutions provided).
• Specimens and sample prep:
  • Coating is applied to filter paper (test sample) at manufacturer’s recommended application rate.
  • Conditioning: typically at (23 ± 2) °C and (50 ± 5) % RH for at least 5 days (EN 23270 referenced).
  • Test specimen diameter: 55 mm; three specimens per condition (with preservative, without preservative, and uncoated substrate).
  • Sterilization: recommended gamma irradiation ≥ 10 kGy (alternative methods may be agreed).
• Apparatus & conditions: incubator at (23 ± 2) °C, sterile work under Class 1 microbiological safety cabinet (EN 12469), lighting ~1 000 ± 200 lx for culture maintenance.
• Outcome: semiquantitative assessment of algicidal efficacy based on algal growth comparison.

Applications - who uses EN 15458:2022

• Paint, varnish and coating manufacturers validating film preservative performance against algae.
• Biocide formulators and suppliers assessing active substances for regulatory dossiers (BPR).
• Independent testing laboratories and QC labs performing standardized algal efficacy tests.
• Product developers for wood and masonry coatings needing evidence of algae control.
• Regulatory bodies and certification agencies referencing standardized test data.

Related standards

• EN 23270 - conditioning temperatures and humidities for paints and varnishes
• EN ISO 1513 - preparation of test samples
• EN ISO 15528 - sampling of paints, varnishes and raw materials
• EN 12469 - microbiological safety cabinet performance criteria

EN 15458:2022 provides a reproducible laboratory protocol for assessing film preservatives, enabling consistent testing, regulatory compliance and product claims related to algae resistance in non‑marine coatings.

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2022
Nadomešča:
SIST EN 15458:2014
Barve in laki - Laboratorijska metoda za preskušanje učinkovitosti konzervansov
filma v premazih proti algam
Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in
a coating against algae
Beschichtungsstoffe - Laborverfahren für die Prüfung der Wirksamkeit von
Filmkonservierungsmitteln in einer Beschichtung gegen Algen
Peintures et vernis - Méthode d'essai en laboratoire permettant de déterminer l'efficacité
des préservateurs du feuil d'un revêtement contre les algues
Ta slovenski standard je istoveten z: EN 15458:2022
ICS:
87.040 Barve in laki Paints and varnishes
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 15458
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2022
EUROPÄISCHE NORM
ICS 87.040 Supersedes EN 15458:2014
English Version
Paints and varnishes - Laboratory method for testing the
efficacy of film preservatives in a coating against algae
Peintures et vernis - Méthode d'essai en laboratoire Beschichtungsstoffe - Laborverfahren für die Prüfung
permettant de vérifier l'efficacité des préservateurs du der Wirksamkeit von Filmkonservierungsmitteln in
feuil d'un revêtement contre les algues einer Beschichtung gegen Algen
This European Standard was approved by CEN on 3 January 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 15458:2022 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Apparatus and materials . 6
6 Microorganisms . 7
7 Sampling and preparation of test samples and of test specimens . 7
7.1 Sampling . 7
7.2 Preparation of test samples (see Annex A) . 7
7.3 Conditioning of the test samples . 7
7.4 Preparation and number of test specimens . 8
8 Procedure. 8
8.1 Preparation of Bold’s Basal Medium . 8
8.2 Preparation of the Bold’s Basal Medium stock solutions . 8
8.3 Preparation of the Petri dishes with culture medium . 9
8.4 Preparation of stock cultures and sub-cultures . 9
8.4.1 Stock cultures: . 9
8.4.2 Sub-cultures: . 9
8.5 Preparation of the algal suspension . 10
8.6 Inoculation and incubation (see Annex A) . 10
8.7 Assessment . 10
9 Test report . 11
Annex A (informative) Laboratory method for testing the efficacy of film preservatives in a
coating against algae . 12
Bibliography . 13

European foreword
This document (EN 15458:2022) has been prepared by Technical Committee CEN/TC 139 “Paints and
varnishes”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2022, and conflicting national standards shall
be withdrawn at the latest by September 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 15458:2014.
The main changes compared to the previous version are as follows:
a) new terms and definitions have been added;
b) the use of terms and definitions throughout the document corrected;
c) the document has been editorially revised and the normative references have been updated.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
Introduction
This document identifies criteria to assess efficacy of film preservatives in a coating against algae. The
results of the method allow evaluation of an active substance with regard to its inclusion in Annex I of the
Biocidal Product Regulation 528/2012 (Regulation EU No 528/2012 of the European Parliament and of
the Council of 22 May 2012 concerning the placing of biocidal products on the market – BPR).
The characteristics of the biocide treated coating material should conform to national regulations with
regard to health, safety and the environment.
1 Scope
This document specifies a laboratory test method for determining the biocidal or biostatic efficacy of
single active substances or combinations thereof used in film preservatives of coatings against algal
growth. The document does not apply to coatings unsusceptible to algal growth. The test method covers
only active substances for film preservation, not the substrate itself, e.g. wood, which is dealt with in
another standard. The test method is applicable for active substances used for wood protection and
masonry coatings. It is not applicable to marine coatings.
Safety, health and environmental aspects are not in the scope of this document.
Determination of the performance of film preservatives in coatings by applying ageing procedures is not
within the scope of this document.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 12469, Biotechnology - Performance criteria for microbiological safety cabinets
EN 23270, Paints and varnishes and their raw materials - Temperatures and humidities for conditioning
and testing (ISO 3270)
EN ISO 1513, Paints and varnishes - Examination and preparation of test samples (ISO 1513)
EN ISO 15528, Paints, varnishes and raw materials for paints and varnishes - Sampling (ISO 15528)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
active substance
substance or micro-organism that has an action on or against harmful organisms
[SOURCE: Biocidal Product Regulation (BPR, Regulation (EU) 528/2012), Article 3.1 (c), modified – the
article “a” between “or” and “micro-organism” was deleted]
3.2
sample
portion of coating material to be tested
3.3
test sample
strip of filter paper without biocidal effect covered with the coating material to be tested
Note 1 to entry: See also Figure A.1.
3.4
test specimen
punched-out portion of a test sample
Note 1 to entry: See also Figure A.1.
4 Principle
To determine the algicidal efficacy of film preservatives in a coating, the coating material is applied to a
substrate, conditioned according to EN 23270, placed onto an agar surface, inoculated with a standard
algal suspension and incubated over a certain period of time under conditions appropriate for algal
growth. Conclusions can be drawn with regard to the algicidal efficacy of the film preservatives in a
coating from the intensity of the algal growth on the coated surface of the test specimen after incubation.
The method described in this document is a semiquantitative, comparative method between coatings
with and without film preservatives.
5 Apparatus and materials
5.1 Cutting device for preparing the test specimens (coated filter paper, with a diameter of 55 mm).
5.2 Autoclave for sterilization.
5.3 Incubator, capable of maintaining (23 ± 2) °C.
5.4 Pipette, in the range between 100 µl to 1 000 µl, with sterile tips or combi-tips of 12,5 ml.
5.5 Filter paper without biocidal effect (e.g. cellulose with a pore size of 0,45 µm and a thickness of
650 µm).
5.6 If applicable, automatic welding apparatus to seal the bags.
5.7 Sterilized glass bottles (100 ml, 0,5 l, 1 l).
5.8 Sterilized test tu
...