EN 15458:2022
(Main)Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae
Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae
This document specifies a laboratory test method for determining the biocidal or biostatic efficacy of single active substances or combinations thereof used in film preservatives of coatings against algal growth. The document does not apply to coatings unsusceptible to algal growth. The test method covers only active substances for film preservation, not the substrate itself, e.g. wood, which is dealt with in another standard. The test method is applicable for active substances used for wood protection and masonry coatings. It is not applicable to marine coatings.
Safety, health and environmental aspects are not in the scope of this document.
Determination of the performance of film preservatives in coatings by applying ageing procedures is not within the scope of this document.
Beschichtungsstoffe - Laborverfahren für die Prüfung der Wirksamkeit von Filmkonservierungsmitteln in einer Beschichtung gegen Algen
Dieses Dokument legt ein Laborverfahren zum Bestimmen der bioziden/biostatischen Wirksamkeit von Wirkstoffen oder einer Kombination aus mehreren Wirkstoffen zur Anwendung in Filmkonservierungsmitteln in Beschichtungen gegen Algenbewuchs fest. Dieses Dokument gilt nicht für Beschichtungen, die unempfindlich gegen Algenbewuchs sind. Das Prüfverfahren deckt nur die Wirkstoffe zur Filmkonservierung der Beschichtung ab, nicht zum Schutz des Substrats, z. B. Holz. Dieser wird in einer anderen Norm behandelt. Das Prüfverfahren ist anwendbar für Wirkstoffe, die in Beschichtungen für den Schutz von Holz und für mineralische Substrate verwendet werden. Die Norm gilt nicht für Beschichtungen im maritimen Bereich.
Sicherheits-, Gesundheits- und Umweltaspekte liegen nicht im Anwendungsbereich dieses Dokuments.
Die Bestimmung der Wirksamkeit von Filmkonservierungsmitteln in Beschichtungen nach Anwendung von Alterungsverfahren liegt nicht im Anwendungsbereich dieses Dokuments.
Peintures et vernis - Méthode d'essai en laboratoire permettant de vérifier l'efficacité des préservateurs du feuil d'un revêtement contre les algues
Le présent document spécifie une méthode d’essai en laboratoire permettant de déterminer l’efficacité biocide ou biostatique de substances actives individuelles, ou de combinaisons de ces substances, utilisées dans les préservateurs du feuil de revêtements contre la prolifération d’algues. Ce document ne s’applique pas aux revêtements qui ne sont pas sensibles à la prolifération d’algues. Cette méthode d’essai concerne uniquement les substances actives utilisées pour la protection du feuil et non du subjectile lui même, par exemple le bois, laquelle fait l’objet d’une autre norme. Cette méthode d’essai est applicable aux substances actives utilisées pour la protection des peintures pour bois et pour maçonnerie. Elle ne s’applique pas aux peintures marines.
Les aspects de sécurité et de santé, ainsi que les aspects environnementaux, ne relèvent pas du domaine d’application du présent document.
La détermination des performances des produits de préservation du feuil de revêtements par des méthodes de vieillissement n’est pas traitée dans le présent document.
Barve in laki - Laboratorijska metoda za preskušanje učinkovitosti konzervansov filma v premazih proti algam
Ta evropski standard določa laboratorijsko preskusno metodo za določanje biocidne/biostatične učinkovitosti posamezne aktivne snovi ali kombinacij aktivnih snovi, ki se uporabljajo v konzervansih filma v premazih proti algam. Standard se ne uporablja za premaze, ki niso občutljivi na alge. Preskusna metoda vključuje samo aktivne snovi za ohranjanje filma in ne za zaščito same podlage, npr. lesa, ki jo obravnava drug standard. Preskusna metoda se uporablja za aktivne snovi, ki se uporabljajo za lesne in zidne premaze. Ne uporablja se za ladijske premaze.
Na področje uporabe tega standarda niso vključeni vidiki varnosti, zdravja in varstva okolja.
Določanje učinkovitosti konzervansov filma v premazih z izvajanjem postopkov staranja ne spada na področje uporabe tega standarda.
General Information
Relations
Overview - EN 15458:2022 (Paints and varnishes)
EN 15458:2022 specifies a laboratory method to determine the biocidal or biostatic efficacy of film preservatives in coatings against algae. It is a semiquantitative, comparative test between coatings with and without film preservatives and is intended for film preservation (not for preservative treatment of substrates). The method supports evaluation of active substances for regulatory assessment (e.g., inclusion considerations under the EU Biocidal Product Regulation, BPR 528/2012). The standard supersedes EN 15458:2014 and applies to coatings susceptible to algal growth such as wood protection and masonry coatings. It is not applicable to marine coatings. Safety, environmental aspects and accelerated ageing procedures are explicitly out of scope.
Key topics and technical requirements
- Test principle: coated filter-paper specimens are conditioned, placed onto agar medium, inoculated with a standard algal suspension and incubated; algal growth intensity on the coated surface is assessed.
- Microorganisms: selects one blue‑green and one green alga from defined strains (examples include Nostoc commune, Gloeocapsa atrata, Klebsormidium flaccidum, Stichococcus bacillaris).
- Culture medium: Bold’s Basal Medium (solid with ~1.5% agar for plates; recipe and stock solutions provided).
- Specimens and sample prep:
- Coating is applied to filter paper (test sample) at manufacturer’s recommended application rate.
- Conditioning: typically at (23 ± 2) °C and (50 ± 5) % RH for at least 5 days (EN 23270 referenced).
- Test specimen diameter: 55 mm; three specimens per condition (with preservative, without preservative, and uncoated substrate).
- Sterilization: recommended gamma irradiation ≥ 10 kGy (alternative methods may be agreed).
- Apparatus & conditions: incubator at (23 ± 2) °C, sterile work under Class 1 microbiological safety cabinet (EN 12469), lighting ~1 000 ± 200 lx for culture maintenance.
- Outcome: semiquantitative assessment of algicidal efficacy based on algal growth comparison.
Applications - who uses EN 15458:2022
- Paint, varnish and coating manufacturers validating film preservative performance against algae.
- Biocide formulators and suppliers assessing active substances for regulatory dossiers (BPR).
- Independent testing laboratories and QC labs performing standardized algal efficacy tests.
- Product developers for wood and masonry coatings needing evidence of algae control.
- Regulatory bodies and certification agencies referencing standardized test data.
Related standards
- EN 23270 - conditioning temperatures and humidities for paints and varnishes
- EN ISO 1513 - preparation of test samples
- EN ISO 15528 - sampling of paints, varnishes and raw materials
- EN 12469 - microbiological safety cabinet performance criteria
EN 15458:2022 provides a reproducible laboratory protocol for assessing film preservatives, enabling consistent testing, regulatory compliance and product claims related to algae resistance in non‑marine coatings.
Standards Content (Sample)
SLOVENSKI STANDARD
01-september-2022
Nadomešča:
SIST EN 15458:2014
Barve in laki - Laboratorijska metoda za preskušanje učinkovitosti konzervansov
filma v premazih proti algam
Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in
a coating against algae
Beschichtungsstoffe - Laborverfahren für die Prüfung der Wirksamkeit von
Filmkonservierungsmitteln in einer Beschichtung gegen Algen
Peintures et vernis - Méthode d'essai en laboratoire permettant de déterminer l'efficacité
des préservateurs du feuil d'un revêtement contre les algues
Ta slovenski standard je istoveten z: EN 15458:2022
ICS:
87.040 Barve in laki Paints and varnishes
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 15458
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2022
EUROPÄISCHE NORM
ICS 87.040 Supersedes EN 15458:2014
English Version
Paints and varnishes - Laboratory method for testing the
efficacy of film preservatives in a coating against algae
Peintures et vernis - Méthode d'essai en laboratoire Beschichtungsstoffe - Laborverfahren für die Prüfung
permettant de vérifier l'efficacité des préservateurs du der Wirksamkeit von Filmkonservierungsmitteln in
feuil d'un revêtement contre les algues einer Beschichtung gegen Algen
This European Standard was approved by CEN on 3 January 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 15458:2022 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Apparatus and materials . 6
6 Microorganisms . 7
7 Sampling and preparation of test samples and of test specimens . 7
7.1 Sampling . 7
7.2 Preparation of test samples (see Annex A) . 7
7.3 Conditioning of the test samples . 7
7.4 Preparation and number of test specimens . 8
8 Procedure. 8
8.1 Preparation of Bold’s Basal Medium . 8
8.2 Preparation of the Bold’s Basal Medium stock solutions . 8
8.3 Preparation of the Petri dishes with culture medium . 9
8.4 Preparation of stock cultures and sub-cultures . 9
8.4.1 Stock cultures: . 9
8.4.2 Sub-cultures: . 9
8.5 Preparation of the algal suspension . 10
8.6 Inoculation and incubation (see Annex A) . 10
8.7 Assessment . 10
9 Test report . 11
Annex A (informative) Laboratory method for testing the efficacy of film preservatives in a
coating against algae . 12
Bibliography . 13
European foreword
This document (EN 15458:2022) has been prepared by Technical Committee CEN/TC 139 “Paints and
varnishes”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2022, and conflicting national standards shall
be withdrawn at the latest by September 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 15458:2014.
The main changes compared to the previous version are as follows:
a) new terms and definitions have been added;
b) the use of terms and definitions throughout the document corrected;
c) the document has been editorially revised and the normative references have been updated.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
Introduction
This document identifies criteria to assess efficacy of film preservatives in a coating against algae. The
results of the method allow evaluation of an active substance with regard to its inclusion in Annex I of the
Biocidal Product Regulation 528/2012 (Regulation EU No 528/2012 of the European Parliament and of
the Council of 22 May 2012 concerning the placing of biocidal products on the market – BPR).
The characteristics of the biocide treated coating material should conform to national regulations with
regard to health, safety and the environment.
1 Scope
This document specifies a laboratory test method for determining the biocidal or biostatic efficacy of
single active substances or combinations thereof used in film preservatives of coatings against algal
growth. The document does not apply to coatings unsusceptible to algal growth. The test method covers
only active substances for film preservation, not the substrate itself, e.g. wood, which is dealt with in
another standard. The test method is applicable for active substances used for wood protection and
masonry coatings. It is not applicable to marine coatings.
Safety, health and environmental aspects are not in the scope of this document.
Determination of the performance of film preservatives in coatings by applying ageing procedures is not
within the scope of this document.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 12469, Biotechnology - Performance criteria for microbiological safety cabinets
EN 23270, Paints and varnishes and their raw materials - Temperatures and humidities for conditioning
and testing (ISO 3270)
EN ISO 1513, Paints and varnishes - Examination and preparation of test samples (ISO 1513)
EN ISO 15528, Paints, varnishes and raw materials for paints and varnishes - Sampling (ISO 15528)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
active substance
substance or micro-organism that has an action on or against harmful organisms
[SOURCE: Biocidal Product Regulation (BPR, Regulation (EU) 528/2012), Article 3.1 (c), modified – the
article “a” between “or” and “micro-organism” was deleted]
3.2
sample
portion of coating material to be tested
3.3
test sample
strip of filter paper without biocidal effect covered with the coating material to be tested
Note 1 to entry: See also Figure A.1.
3.4
test specimen
punched-out portion of a test sample
Note 1 to entry: See also Figure A.1.
4 Principle
To determine the algicidal efficacy of film preservatives in a coating, the coating material is applied to a
substrate, conditioned according to EN 23270, placed onto an agar surface, inoculated with a standard
algal suspension and incubated over a certain period of time under conditions appropriate for algal
growth. Conclusions can be drawn with regard to the algicidal efficacy of the film preservatives in a
coating from the intensity of the algal growth on the coated surface of the test specimen after incubation.
The method described in this document is a semiquantitative, comparative method between coatings
with and without film preservatives.
5 Apparatus and materials
5.1 Cutting device for preparing the test specimens (coated filter paper, with a diameter of 55 mm).
5.2 Autoclave for sterilization.
5.3 Incubator, capable of maintaining (23 ± 2) °C.
5.4 Pipette, in the range between 100 µl to 1 000 µl, with sterile tips or combi-tips of 12,5 ml.
5.5 Filter paper without biocidal effect (e.g. cellulose with a pore size of 0,45 µm and a thickness of
650 µm).
5.6 If applicable, automatic welding apparatus to seal the bags.
5.7 Sterilized glass bottles (100 ml, 0,5 l, 1 l).
5.8 Sterilized test tu
...
Frequently Asked Questions
EN 15458:2022 is a standard published by the European Committee for Standardization (CEN). Its full title is "Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae". This standard covers: This document specifies a laboratory test method for determining the biocidal or biostatic efficacy of single active substances or combinations thereof used in film preservatives of coatings against algal growth. The document does not apply to coatings unsusceptible to algal growth. The test method covers only active substances for film preservation, not the substrate itself, e.g. wood, which is dealt with in another standard. The test method is applicable for active substances used for wood protection and masonry coatings. It is not applicable to marine coatings. Safety, health and environmental aspects are not in the scope of this document. Determination of the performance of film preservatives in coatings by applying ageing procedures is not within the scope of this document.
This document specifies a laboratory test method for determining the biocidal or biostatic efficacy of single active substances or combinations thereof used in film preservatives of coatings against algal growth. The document does not apply to coatings unsusceptible to algal growth. The test method covers only active substances for film preservation, not the substrate itself, e.g. wood, which is dealt with in another standard. The test method is applicable for active substances used for wood protection and masonry coatings. It is not applicable to marine coatings. Safety, health and environmental aspects are not in the scope of this document. Determination of the performance of film preservatives in coatings by applying ageing procedures is not within the scope of this document.
EN 15458:2022 is classified under the following ICS (International Classification for Standards) categories: 87.040 - Paints and varnishes. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 15458:2022 has the following relationships with other standards: It is inter standard links to EN 15458:2014. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase EN 15458:2022 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.
記事のタイトル：EN 15458:2022 - 塗料とワニス - 藻類に対するコーティング内のフィルム保存剤の効果をテストするための実験室での方法 記事の内容：この文書では、藻類の成長に対してコーティング内のフィルム保存剤の一つまたはそれらの組み合わせの生物殺虫剤または生物静止剤の効力を測定するための実験室でのテスト方法を示しています。この文書は藻類の成長に対して耐性のあるコーティングには適用されません。このテスト方法はフィルム保存剤の活性成分のみを対象とし、木材などの基材自体には別の基準が適用されます。このテスト方法は木材保護やれんが塗装に使用される活性成分に適用できますが、海洋用の塗料には適用できません。 安全性、健康、環境への影響は、この文書の範囲外です。 フィルム保存剤の性能を年齢化プロセスによって評価することは、この文書の範囲内に含まれていません。
記事タイトル：EN 15458:2022 - ペイントおよびニス - コーティング剤中の藻類に対するフィルム防腐剤の効果を試験するための実験室方法 記事内容：この文書では、コーティング剤中のフィルム防腐剤が藻類の生育に対して殺菌または阻菌効果を持つかどうかを評価するための実験室試験方法を規定しています。この文書は、藻類の生育に対して影響を受けないコーティング剤には適用されません。この試験方法は、フィルム防腐剤の活性成分にのみ適用され、木材などの基材自体は別の規格で扱われます。この試験方法は、木材保護および石造りのコーティング剤に使用される活性成分に適用されます。ただし、船舶用コーティング剤には適用されません。 安全性、健康、環境の側面は、この文書の範囲に含まれていません。 エージング手順を適用してコーティング剤中のフィルム防腐剤の性能を評価することは、この文書の範囲外です。
기사 제목: EN 15458:2022 - 도료 - 코팅 내 부창류에 대한 필름 방부제의 효능 시험을 위한 실험실 방법 기사 내용: 이 문서는 부창성 성장에 대한 화학적 처리 또는 생화학적 처리를 사용하는 필름 방부제의 효능을 결정하기 위한 실험실 시험 방법을 명시하고 있다. 이 문서는 부창성 성장이 불가능한 코팅에는 적용되지 않는다. 이 시험 방법은 필름 방부제의 활성 성분만 다루며, 예를 들어 나무와 같은 기재는 다른 표준에서 다룬다. 이 시험 방법은 나무 보호 및 석조 코팅에 사용되는 활성 성분에 적용할 수 있으며, 해양용 코팅에는 적용할 수 없다. 안전, 보건 및 환경 측면은 이 문서의 범위에 포함되지 않는다. 시험법에 의한 코팅의 필름 방부제 성능 결정은 이 문서의 범위에 포함되지 않는다.
The article discusses EN 15458:2022, which is a laboratory test method for evaluating the effectiveness of film preservatives in coatings against algae growth. The test method is specifically for active substances used in coatings, not the substrate itself. It is applicable for wood protection and masonry coatings, but not for marine coatings. Safety, health, environmental aspects, and performance evaluation through ageing procedures are not covered in this document.
The article discusses EN 15458:2022, a laboratory test method for testing the effectiveness of film preservatives in coatings against algae growth. The test method specifically focuses on the biocidal or biostatic efficacy of single active substances or combinations of substances used in film preservatives. This standard does not apply to coatings that are resistant to algae growth, and it does not cover the substrate itself (such as wood), which is addressed in another standard. The test method is suitable for active substances used in wood protection and masonry coatings, but it is not applicable to marine coatings. Safety, health, and environmental aspects are not considered in this document, and it does not cover the evaluation of the preservative performance through aging procedures.
제목: EN 15458:2022 - 도료 및 발색제 - 도말 성능보존제에 대한 담수조류에 대한 경화 효능을 실험하는 실험실 방법 내용: 이 문서는 도말 성능보존제의 단일 활성 성분 또는 그 조합의 담수조류 성장에 대한 살균 또는 저균능을 결정하는 실험실 시험 방법을 명시한다. 이 문서는 담수조류 성장이 없는 도료에는 적용되지 않는다. 이 시험 방법은 도말 보존을 위해 사용되는 활성 성분에만 적용되며, 다른 표준에서 다루는 목재와 같은 기재 자체에는 적용되지 않는다. 이 시험 방법은 목재 보호 및 석조도료에 사용되는 활성 성분에 적용된다. 그러나 해양도장에는 적용되지 않는다. 안전, 보건 및 환경 측면은 이 문서의 범위에 포함되지 않는다. 시험 절차를 통해 도말 성능보존제의 성능을 결정하는 것은 이 문서의 범위에 포함되지 않는다.
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