SIST EN ISO 15301:2001

• Abstract:

  Method for the determination of sediments in crude oil by centrifugation.

• Status: Published
• Publication Date: 30-Nov-2001
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 01-Dec-2001
• Due Date: 01-Dec-2001
• Completion Date: 01-Dec-2001
• Ref Project: EN ISO 15301:2001 - Animal and vegetable fats and oils - Determination of sediment in crude fats and oils - Centrifuge method (ISO 15301:2001)

• Refers: SIST ISO 661:1995 - Animal and vegetable fats and oils -- Preparation of test sample
• Effective Date: 25-Aug-2026
• Refers: SIST EN 12544-1:2000 - Non-destructive testing - Measurement and evaluation of the X-ray tube voltage - Part 1: Voltage divider method
• Effective Date: 28-Jan-2026
• Corrected By: SIST EN ISO 15301:2001/AC:2007 - Animal and vegetable fats and oils - Determination of sediment in crude fats and oils - Centrifuge method (ISO 15301:2001/Cor 1:2007)
• Effective Date: 22-Dec-2008

SIST EN ISO 15301:2001 is the Slovenian adoption of EN ISO 15301:2001, an ISO method for measuring sediment in crude animal and vegetable fats and oils by centrifugation. It tells laboratories how to prepare the sample, run the centrifuge test, read the sediment layer, and calculate the sediment content for crude oils and fats obtained by extraction and/or crushing.

What does SIST EN ISO 15301:2001 specify?

SIST EN ISO 15301:2001 specifies a laboratory method for determining the sediment in crude fats or oils that can be separated by centrifugal force. It is intended for crude oils in the stated sediment range and is not used for fats that are not liquid at 20 C.

The document is organized into clauses 1 to 11 covering scope, normative reference, term and definition, principle, apparatus, sampling, sample preparation, procedure, expression of results, precision, and test report. It also includes annexes for calculation, precision evidence, and European reference mapping.

Annex	What it covers
Annex A	Calculation of the rotational frequency of the centrifuge
Annex B	Interlaboratory test results and comparison with the 96 h method
Annex ZA	Normative references to international publications with their European publications

What are the key requirements of SIST EN ISO 15301:2001?

SIST EN ISO 15301:2001 is a duplicate-laboratory centrifuge test. The most important requirements are sample preparation, controlled centrifuging, reading the sediment layer correctly, and reporting the result in a traceable way.

Sample handling and preparation

Clause 6 says the laboratory should receive a representative sample that has not been changed during transport or storage, and the sample should be stored in glass or polyethylene terephthalate (PET) bottles. Clause 7 requires preparation of the test sample in accordance with ISO 661, then warming it if needed to 20 C to 25 C and redistributing any settled material before testing. In practice, this matters because the sediment must be part of the test portion, not left behind in the bottle.

Centrifuge setup and tubes

Clause 5 specifies 100 ml centrifuge tubes that are pear-shaped or cone-shaped, made of thoroughly annealed glass and fitted with a stopper, together with fat- and oil-resistant buckets and a controllable centrifuge. The centrifuge must deliver the stated radial acceleration at the narrow part of the tubes, and Annex A gives the calculation for rotational frequency from the swing diameter. For the user, this means the centrifuge settings are part of the method and must be known, not guessed.

Procedure and reading the result

Clause 8 calls for two tubes, each filled with 100 ml of prepared test sample, then centrifuged for 1 h 5 s. The sediment volume is read from the tube, with different reading accuracy depending on the amount, and the result should be corrected if separation is incomplete in a pear-shaped tube. The method also requires recording the centrifuge acceleration or swing diameter and frequency, plus the temperature before and after centrifuging, so the test can be checked later.

Result expression and precision

Clause 9 converts the measured sediment volume into sediment content using the tube mass and the test portion mass, then averages the two tube results and reports the final value to the nearest 1 ml per 100 g. Clause 10 gives repeatability and reproducibility limits based on interlaboratory testing, with details in Annex B. In practice, this tells the laboratory how to judge whether two results are close enough to agree.

Test report

Clause 11 lists the information that must be recorded in the report: sample identification, known sampling method, method reference, centrifuge settings, temperatures, any nonstandard operating details or incidents, and the result or the two results if repeatability was checked. That makes the result usable for quality control, dispute checking, or comparison with another laboratory.

What terms does SIST EN ISO 15301:2001 define?

Sediment is the insoluble material in a crude fat or oil that can be separated by centrifuging and collected as the unclear layer at the bottom of the tube.

Crude fats or oils are the materials to which the method applies when they are obtained by extraction and/or crushing and remain liquid at 20 C.

Relative radial acceleration is the centrifugal acceleration used to set the centrifuge performance at the narrow part of the tube.

Repeatability is the closeness of two results obtained in the same laboratory by the same operator with the same equipment over a short time.

Reproducibility is the closeness of two results obtained in different laboratories by different operators using different equipment.

Test report is the written record of the sample, method, settings, temperatures, observations, and final result.

Who uses SIST EN ISO 15301:2001?

SIST EN ISO 15301:2001 is used by laboratories testing crude animal and vegetable fats and oils, especially in the fats-and-oils and oilseed sectors. It is relevant to quality-control staff, laboratory managers, and analysts who need a fast check of sediment after extraction or crushing. It is also useful where results must be compared between laboratories or documented for trade and internal quality control.

Which standards are used with SIST EN ISO 15301:2001?

ISO 661 is the main normative reference and gives the preparation of the test sample. ISO 5555 is cited for recommended sampling, even though sampling itself is outside the method. ISO 5725-2 is used for the interlaboratory testing approach reported in Annex B, and Annex ZA maps the international references to their European publications.

What does the SIST EN ISO 15301:2001 document contain?

• Two tube drawings: a pear-shaped tube and a cone-shaped tube.
• A detailed procedure for weighing tubes, adding 100 ml of sample, centrifuging, and reading the sediment layer.
• Annex A with the calculation for centrifuge rotational frequency.
• Annex B with interlaboratory precision tables and a comparison with the 96 h standing method.
• A test-report list that supports routine testing, method validation, and audit trail documentation.

SLOVENSKI STANDARD
01-december-2001
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHXVHGOLQHYVXURYLKPDãþREDK
LQROMLK0HWRGDFHQWULIXJH,62
Animal and vegetable fats and oils - Determination of sediment in crude fats and oils -
Centrifuge method (ISO 15301:2001)
Tierische und pflanzliche Fette und Öle - Bestimmung des Sedimentgehaltes in rohen
Fetten und Ölen - Zentrifugenverfahren (ISO 15301:2001)
Corps gras d'origines animale et végetale - Détermination de la teneur en sédiment dans
des corps gras bruts - Méthode par centrifugation (ISO 15301:2001)
Ta slovenski standard je istoveten z: EN ISO 15301:2001
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 15301
NORME EUROPÉENNE
EUROPÄISCHE NORM
September 2001
ICS 67.200.10
English version
Animal and vegetable fats and oils - Determination of sediment
in crude fats and oils - Centrifuge method (ISO 15301:2001)
Corps gras d'origines animale et végetale - Détermination Tierische und pflanzliche Fette und Öle - Bestimmung des
de la teneur en sédiment dans des corps gras bruts - Sedimentgehaltes in rohen Fetten und Ölen -
Méthode par centrifugation (ISO 15301:2001) Zentrifugenverfahren (ISO 15301:2001)
This European Standard was approved by CEN on 1 September 2001.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2001 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15301:2001 E
worldwide for CEN national Members.

CORRECTED 2001-11-07
Foreword
The text of the International Standard ISO 15301:2001 has been prepared by Technical
Committee ISO/TC 34 "Agricultural food products" in collaboration with Technical Committee
CEN/TC 307 "Oilseeds, vegetable and animal fats and oils and their by-products - Methods of
sampling and analysis", the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by March 2002, and conflicting national
standards shall be withdrawn at the latest by March 2002.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United
Kingdom.
Endorsement notice
The text of the International Standard ISO 15301:2001 was approved by CEN as a European
Standard without any modification.
NOTE: Normative references to International Standards are listed in annex ZA (normative).
Annex ZA (normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions
of -any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications,
indicated by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 661 1989 Animal and vegetabie fats and oils EN ISO 661 1995
— Preparation of test sample
INTERNATIONAL ISO
STANDARD 15301
First edition
2001-09-01
Animal and vegetable fats and oils —
Determination of sediment in crude fats and
oils — Centrifuge method
Corps gras d'origines animale et végétale — Détermination de la teneur en
sédiment dans des corps gras bruts — Méthode par centrifugation
Reference number
ISO 15301:2001(E)
ISO 15301:2001(E)
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ii ISO 2001 – All rights reserved

ISO 15301:2001(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
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Commission (IEC) on all matters of electrotechnical standardization.
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Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 15301 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 11, Animal and vegetable fats and oils.
Annexes A and B of this International Standard are for information only.
©
ISO 2001 – All rights reserved iii

INTERNATIONAL STANDARD ISO 15301:2001(E)
Animal and vegetable fats and oils — Determination of sediment in
crudefatsandoils —Centrifugemethod
1 Scope
This International Standard specifies a method for the determination in crude fats or oils of that sediment which can
be separated by centrifugal force.
The method is applicable to crude oils and to oils with a sediment content of 0,03 ml per 100 g to 15 ml per 100 g,
obtained by means of extraction and/or crushing.
�
The method is not applicable to fats which are not liquid at a temperature of 20 C.
2 Normative reference
The following normative document contains provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these publica-
tions do not apply. However, parties to agreements based on this International Standard are encouraged to investi-
gate the possibility of applying the most recent edition of the normative document indicated below. For undated
references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain reg-
isters of currently valid International Standards.
ISO 661:1989, Animal and vegetable fats and oils — Preparation of test sample
3 Term and definition
For the purposes of this International Standard, the following term and definition applies.
3.1
sediment
that part of the insoluble matter in a crude fat or oil which can be centrifugally separated and is the total amount of
the unclear layer of components collected at the bottom of the measuring tube after centrifuging
NOTE The sediment contains, for example
...