SIST EN ISO 13884:2005

Animal and vegetable fats and oils - Determination of isolated trans isomers by infrared spectrometry (ISO 13884:2003)

ISO 13884:2003 specifies a method for the accurate determination of isolated trans bonds in natural or processed long-chain fatty acids, fatty acid esters and triglycerides with trans isomer levels greater than or equal to 5 %.
The method is not applicable, or is applicable only with specific precautions, to fats and oils containing high levels (over 5 %) of conjugated unsaturation (e.g. tung oil); materials containing functional groups which modify the intensity of the C-H deformation about the trans double bond [e.g. castor oil containing ricinoleic acid or its geometrical isomer, ricinelaidic acid (12-hydroxy-9-octadecenoic acid)]; mixed triglycerides having long- and short-chain moieties (such as diacetostearin); or in general, any materials containing constituents which have functional groups which give rise to specific absorption bands at or sufficiently close to interfere with the 966 cm-1 band of the C-H deformation of the isolated trans double bond.

Tierische und pflanzliche Fette und Öle - Bestimmung isolierter trans-Isomere durch Infarot-Spektrometrie (ISO 13884:2003)

Diese Internationale Norm legt ein Verfahren für die genaue Bestimmung isolierter trans-Bindungen in natürlichen oder bearbeiteten langkettigen Fettsäuren, Fettsäureestern und Triglyceriden mit einem Gehalt an trans-Isomeren > 5 % fest.
Das Verfahren ist nicht oder nur mit besonderen Vorkehrungen anwendbar auf:
-   Fette und Öle mit hohem Gehalt (über 5 %) an ungesättigten konjugierten Bindungen (z. B. Tungöl);
-   Substanzen mit funktionellen Gruppen, die die Intensität der C H Verformung um die trans-Doppelbindung verändern [z. B. Ricinolsäure oder deren geometrisches Isomer Ricinelaidinsäure (12 Hydroxy-9 Octadecensäure) enthaltendes Rizinusöl];gemischte Triglyceride mit lang  und kurzkettigen Anteilen (wie Diacetostearin), oder im Allgemeinen;
-   alle Substanzen mit Bestandteilen mit funktionellen Gruppen, die spezifische Extinktionsbanden bei oder ausreichend nahe der 966 cm-1 Bande der C H Verformung durch die isolierte trans-Doppelbindung erzeugen und diese stören.
ANMERKUNG   Diene wie cis trans  und trans trans Diene können die Kalibrierung beeinträchtigen.
Für genaue Bestimmungen bei Substanzen mit einem Gehalt an trans Bindungen unter 5 % wird ein genormtes gaschromatographisches Verfahren mit einer Kapillarsäule empfohlen (siehe zum Beispiel ISO 15304).

Corps gras d'origines animale et végétale - Détermination, par spectrométrie infrarouge, des isomeres trans isolés (ISO 13884:2003)

L'ISO 13884:2003 spécifie une méthode pour la détermination précise des liaisons trans isolées dans les acides gras à longue chaîne, dans les esters d'acides gras et dans les triglycérides ayant des niveaux d'isomères trans égaux ou supérieurs à 5 %, naturels ou de synthèse.
La méthode n'est pas applicable, ou n'est applicable qu'en prenant certaines précautions, aux graisses et huiles contenant des niveaux élevés (supérieurs à 5 %) d'insaturés conjugués (par exemple l'huile de bois de Chine); aux matériaux contenant des groupes fonctionnels modifiant l'intensité de la déformation C-H sur la double liaison trans [par exemple l'huile de ricin qui contient de l'acide ricinoléique ou son isomère géométrique, l'acide ricinélaïdique (acide 12-hydroxy-9-octadécénoïque)]; aux triglycérides mixtes ayant des portions à chaîne longue et courte (par exemple la diacétostéarine), ni, en général, à tout matériau contenant des composants ayant des groupes fonctionnels qui donnent lieu à des bandes d'absorption spécifiques situées sur la bande 966 cm-1 de la déformation C-H de la double liaison trans isolée ou étant suffisamment proche pour interférer avec cette bande.

Rastlinske in živalske maščobe in olja – Določevanje izoliranih trans izomer z infrardečo spektrometrijo (ISO 13884:2003)

General Information

• Status: Published
• Publication Date: 30-Nov-2005
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 01-Dec-2005
• Due Date: 01-Dec-2005
• Completion Date: 01-Dec-2005
• Ref Project: EN ISO 13884:2005 - Animal and vegetable fats and oils - Determination of isolated trans isomers by infrared spectrometry (ISO 13884:2003)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 13884:2005
01-december-2005
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD±'RORþHYDQMHL]ROLUDQLKWUDQVL]RPHU]
LQIUDUGHþRVSHNWURPHWULMR,62
Animal and vegetable fats and oils - Determination of isolated trans isomers by infrared
spectrometry (ISO 13884:2003)
Tierische und pflanzliche Fette und Öle - Bestimmung isolierter trans-Isomere durch
Infarot-Spektrometrie (ISO 13884:2003)
Corps gras d'origines animale et végétale - Détermination, par spectrométrie infrarouge,
des isomeres trans isolés (ISO 13884:2003)
Ta slovenski standard je istoveten z: EN ISO 13884:2005
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 13884:2005 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 13884:2005

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SIST EN ISO 13884:2005
EUROPEAN STANDARD
EN ISO 13884
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2005
ICS 67.200.10

English Version
Animal and vegetable fats and oils - Determination of isolated
trans isomers by infrared spectrometry (ISO 13884:2003)
Corps gras d'origines animale et végétale - Détermination, Tierische und pflanzliche Fette und Öle - Bestimmung
par spectrométrie infrarouge, des isomères trans isolés isolierter trans-Isomere durch Infarot-Spektrometrie (ISO
(ISO 13884:2003) 13884:2003)
This European Standard was approved by CEN on 28 July 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 13884:2005: E
worldwide for CEN national Members.

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SIST EN ISO 13884:2005
EN ISO 13884:2005 (E)






Foreword



The text of ISO 13884:2003 has been prepared by Technical Committee ISO/TC 34 "Agricultural
food products” of the International Organization for Standardization (ISO) and has been taken
over as EN ISO 13884:2005 by Technical Committee CEN/TC 307 "Oilseeds, vegetable and
animal fats and oils and their by-products - Methods of sampling and analysis", the secretariat of
which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by February 2006, and conflicting national
standards shall be withdrawn at the latest by February 2006.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.



Endorsement notice

The text of ISO 13884:2003 has been approved by CEN as EN ISO 13884:2005 without any
modifications.

2

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SIST EN ISO 13884:2005


INTERNATIONAL ISO
STANDARD 13884
First edition
2003-07-01


Animal and vegetable fats and oils —
Determination of isolated trans isomers
by infrared spectrometry
Corps gras d'origines animale et végétale — Détermination, par
spectrométrie infrarouge, des isomères trans isolés




Reference number
ISO 13884:2003(E)
©
ISO 2003

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SIST EN ISO 13884:2005
ISO 13884:2003(E)
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ii © ISO 2003 — All rights reserved

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SIST EN ISO 13884:2005
ISO 13884:2003(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 13884 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
© ISO 2003 — All rights reserved iii

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SIST EN ISO 13884:2005

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SIST EN ISO 13884:2005
INTERNATIONAL STANDARD ISO 13884:2003(E)

Animal and vegetable fats and oils — Determination of isolated
trans isomers by infrared spectrometry
1 Scope
This International Standard specifies a method for the accurate determination of isolated trans bonds in
natural or processed long-chain fatty acids, fatty acid esters and triglycerides with trans isomer levels W 5 %.
The method is not applicable, or is applicable only with specific precautions, to
 fats and oils containing high levels (over 5 %) of conjugated unsaturation (e.g. tung oil),
 materials containing functional groups which modify the intensity of the C-H deformation about the trans
double bond [e.g. castor oil containing ricinoleic acid or its geometrical isomer, ricinelaidic acid
(12-hydroxy-9-octadecenoic acid)],
 mixed triglycerides having long- and short-chain moieties (such as diacetostearin), or in general,
 any materials containing constituents which have functional groups which give rise to specific absorption
–1
bands at or sufficiently close to interfere with the 966 cm band of the C-H deformation of the isolated
trans double bond.
NOTE Dienes, such as cis-trans and trans-trans dienes, can affect the calibration.
For accurate determinations on materials with trans levels below 5 %, a standardized capillary GLC method is
recommended (for example, see ISO 15304).
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 661, Animal and vegetable fats and oils — Preparation of test sample
ISO 5509, Animal and vegetable fats and oils — Preparation of methyl esters of fatty acids
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
percentage of trans fatty acids
% trans
quantity of those substances in the sample, expressed in terms of the mass equivalent of methyl elaidate,
divided by the sample mass expressed in grams per 10 ml of solvent
© ISO 2003 — All rights reserved 1

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SIST EN ISO 13884:2005
ISO 13884:2003(E)
4 Principle
In most naturally occurring vegetable fats and oils, unsaturated constituents contain only isolated (i.e. non-
conjugated) double bonds in the cis configuration. These cis bonds may be isomerized to the trans
configuration during extraction and processing procedures, due to oxidation, conversion during heating, and/or
partial hydrogenation. Animal and marine fats and oils may contain measurable amounts of naturally occurring
trans isomers. Isolated trans bonds in long-chain fatty acids, fatty acid esters and triglycerides may be
–1
measured by infrared (IR) spectrometry. An absorption band with a maximum at 966 cm (10,3 µm), arising
from a C-H deformation about a trans double bond, is exhibited in the spectra of all compounds containing an
isolated trans group. This band is not observed in the spectra of the corresponding cis and saturated
compounds. Measurement of the intensity of this absorption band under analytically controlled conditions is
the basis for a quantitative method for the determination of the isolated trans isomer content. For higher
accuracy, common interfering absorptions associated with the glycerol backbone of triglycerides and the
carboxyl groups of fatty acids must be eliminated by conversion of these samples to their methyl esters prior
to analysis.
5 Reagents
Use only reagents of recognized analytical grade, unless otherwise specified.
5.1 Carbon disulfide (CS ), dry.
2
WARNING — Prolonged breathing of carbon disulfide vapours is dangerous. This solvent should be
handled only under conditions that provide adequate ventilation, preferably under a fume hood.
1)
5.2 Primary standards: methyl elaidate and methyl oleate, with a purity of 99 % .
6 Apparatus
–1
6.1 Infrared spectrometer (FT-IR or dispersive), capable of making measurements at 4 cm resolution in
–1 –1
the spectral range covering 1 050 cm to 900 cm .
The data-handling capability of the instrument should allow conversion of spectra to absorbance, scale
–1
expansion of the x- and y-axes, and readout of wave numbers to the nearest 1 cm and of absorbance to the
nearest 0,001 AU. FT-IR spectrometers should use TGS or DTGS detectors, or ensure linearity.
6.2 Infrared liquid sampling cells, with NaCl or KBr windows and a fixed optical path length of 1 mm.
For use in null-type instruments, pairs of cells matched to within 0,01 AU are required. In split-beam type
instruments with both cells filled with the solvent (5.1), electronic balance of the two beams to within these
limits should be attained.
...