SIST EN 16963:2018

Fertilizers - Determination of boron, cobalt, copper, iron, manganese, molybdenum and zinc using ICP-AES

This European Standard specifies a method for the determination of boron, cobalt, copper, iron, manganese, molybdenum and zinc in fertilizer extracts using inductively coupled plasma-atomic emission spectrometry (ICP-AES).
This method is applicable to water and aqua regia fertilizer extracts prepared according to EN 16962 and/or EN 16964.
NOTE   In most cases, the presence of small quantities of organic matter will not affect determinations by ICP-AES and it is not necessary to apply organic matter removal.

Düngemittel - Bestimmung von Bor, Cobalt, Kupfer, Eisen, Mangan, Molybdän und Zink mit ICP-AES

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Bor, Cobalt, Kupfer, Eisen, Mangan, Molybdän und Zink in Düngemittelextrakten mittels Atomemissionsspektrometrie mit induktiv gekoppeltem Plasma (ICP AES) fest.
Dieses Verfahren ist auf Wasser- und Königswasserextrakte von Düngemitteln anwendbar, die nach EN 16962 und/oder EN 16964 erhalten wurden.
ANMERKUNG   In den meisten Fällen wird das Vorhandensein geringer Mengen organischer Stoffe die Bestimmung mit ICP AES nicht beeinträchtigen, und es ist daher nicht notwendig, die Beseitigung organischer Verbindungen anzuwenden.

Engrais - Dosage du bore, du cobalt, du cuivre, du fer, du manganèse, du molybdène et du zinc par ICP-AES

La présente norme européenne spécifie une méthode pour le dosage du bore, du cobalt, du cuivre, du fer, du manganèse, du molybdène et du zinc dans les extraits d’engrais par spectrométrie d’émission atomique par un plasma à couplage inductif (ICP-AES).
Cette méthode est applicable aux extraits d’engrais aqueux et à l’eau régale préparés conformément à l’EN 16962 et/ou à l’EN 16964.
NOTE   Dans la plupart des cas, la présence de petites quantités de matière organique n’a aucune incidence sur les dosages par ICP-AES et il n’est pas nécessaire d’appliquer l’élimination de la matière organique.

Gnojila - Določevanje bora, kobalta, bakra, železa, mangana, molibdena in cinka z atomsko emisijsko spektrometrijo z induktivno sklopljeno plazmo (ICP/AES)

Ta evropski standard določa metodo za določevanje bora, kobalta, bakra, železa, mangana, molibdena in cinka v ekstraktih gnojila z atomsko emisijsko spektrometrijo z induktivno sklopljeno plazmo (ICP-AES).
Ta metoda se uporablja za ekstrakte gnojil v vodi in zlatotopki, pripravljene v skladu s standardom prEN 16962 in/ali prEN 16964.
OPOMBA:   V večini primerov prisotnost majhnih količin organske snovi ne bo vplivala na določitve ICP-AES in odstranjevanje organske snovi ni nujno.

General Information

• Status: Published
• Public Enquiry End Date: 05-Apr-2016
• Publication Date: 17-May-2018
• ICS: 65.080 - Fertilizers
• Technical Committee: KAT - Soil quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 04-May-2018
• Due Date: 09-Jul-2018
• Completion Date: 18-May-2018
• Directive: 2003/2003 - Regulation (EC) N° 2003/2003 of the European Parlament and of the council of 13 October 2003 relating to fertilisers
• Directive: 2019/1009 - Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down rules on the making available on the market of EU fertilising products and amending Regulations (EC) No 1069/2009 and (EC) No 1107/2009 and repealing Regulation (EC) No 2003/2003
• Mandate: M/335 - Standardization Mandate assigned to the CEN concerning the modernisation of the methods of anylysis of Fertilizers
• Ref Project: EN 16963:2018 - Fertilizers - Determination of boron, cobalt, copper, iron, manganese, molybdenum and zinc using ICP-AES

Overview

EN 16963:2018 is a CEN European Standard that specifies an analytical method for the determination of boron, cobalt, copper, iron, manganese, molybdenum and zinc in fertilizers using inductively coupled plasma–atomic emission spectrometry (ICP‑AES). The method applies to water and aqua regia fertilizer extracts prepared according to EN 16962 and EN 16964. EN 16963:2018 defines sample preparation, calibration, measurement and reporting practices for reliable multi‑element micro‑nutrient analysis in fertilizer quality control and regulatory testing.

Key Topics

• Scope and principle: ICP‑AES measurement of element‑specific emission lines from fertilizer extracts after appropriate dilution.
• Applicable extracts: Water soluble and aqua regia extracts (per EN 16962 / EN 16964).
• Interferences and matrix effects:
  • Spectral interferences (line overlaps, background) - use alternative lines or software correction.
  • Transport and excitation interferences - mitigate by dilution, matrix‑matching, internal standards or optimization of plasma conditions.
  • Chemical and memory interferences - recognize boron memory effects and apply adequate rinse procedures.
• Reagents & consumables:
  • High‑purity water (EN ISO 3696), nitric and hydrochloric acids, mixed acid matrix, certified multi‑element standard solutions, argon gas for plasma.
• Apparatus & operating modes:
  • ICP‑AES instrument with nebulizer and spray chamber; axial or radial viewing; sequential or simultaneous optics.
• Procedure essentials:
  • Dilution strategy to place analyte concentration mid‑range of calibration, preparation of blank solutions, calibration solutions, spiking for recovery checks.
• Quality and performance:
  • Calibration, blank control, spiking, precision estimates and inter‑laboratory study results are included to support method performance and reproducibility.

Applications and Users

EN 16963:2018 is designed for practical use in:

• Fertilizer manufacturers performing in‑house quality control and micro‑nutrient specification testing.
• Independent testing laboratories and certification bodies verifying compliance with fertilizer regulations.
• Regulatory agencies requiring harmonized analytical methods for market surveillance.
• Environmental and agronomy labs analyzing nutrient content for agronomic recommendations.

Keywords: EN 16963:2018, fertilizer analysis standard, ICP‑AES method, micro‑nutrient determination, boron cobalt copper iron manganese molybdenum zinc, water extract, aqua regia extract.

Related Standards

• EN 16962 - Extraction of water soluble micro‑nutrients and removal of organics
• EN 16964 - Extraction of total micro‑nutrients using aqua regia
• EN ISO 3696 - Water for analytical laboratory use
• EN 12944 (parts 1 & 2) - Fertilizer terminology

This standard supports consistent, validated ICP‑AES testing workflows to ensure accurate micro‑nutrient measurement in fertilizers for quality control and regulatory compliance.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.,&3$(6Düngemittel - Bestimmung von Bor, Cobalt, Kupfer, Eisen, Mangan, Molybdän und Zink mit ICP-AESEngrais - Dosage du bore, du cobalt, du cuivre, du fer, du manganèse, du molybdène et du zinc par ICP-AESFertilizers - Determination of boron, cobalt, copper, iron, manganese, molybdenum and zinc using ICP-AES65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 16963:2018SIST EN 16963:2018en,fr,de01-junij-2018SIST EN 16963:2018SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16963
January
t r s z ICS
x wä r z r English Version
Fertilizers æ Determination of boroná cobaltá copperá ironá manganeseá molybdenum and zinc using ICPæAES Engrais æ Dosage du boreá du cobaltá du cuivreá du ferá du manganèseá du molybdène et du zinc par ICPæAES
Düngemittel æ Bestimmung von Borá Cobaltá Kupferá Eisená Manganá Molybdän und Zink mit ICPæAES This European Standard was approved by CEN on
s w October
t r s yä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Serbiaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Rue de la Science 23,
B-1040 Brussels
t r s z CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s x { x uã t r s z ESIST EN 16963:2018

Statistical results of the inter-laboratory test . 17 Bibliography . 32
10 % as well as contents of > 10 %. The inter-laboratory study reflects the final properties of the method for determination of individual micro-nutrients in water and aqua regia extracts including extraction steps. WARNING — Persons using this European Standard should be familiar with normal laboratory practice. This European Standard does not purport to address all of the safety issues, if any, associated with its use. It is the responsibility of the user to establish appropriate health and safety practices and to ensure compliance with any national regulatory conditions. IMPORTANT — It is absolutely essential that tests conducted according to this European Standard are carried out by suitably trained staff. SIST EN 16963:2018

= 1,4 g/ml. 6.2.1 Diluted nitric acid solution, c(HNO3) = 5 mol/l. Add 350 ml of nitric acid (6.2) to 650 ml of water (6.1). 6.2.2 Diluted nitric acid solution, c(HNO3) = 0,5 mol/l. Dilute 35 ml nitric acid (6.2.) to 1 l with water (6.1). 6.3 Hydrochloric acid, c(HCl) = 12 mol/l;
= 1,18 g/ml. 6.4 Mixed solution, 0,8 mol/l nitric acid and 1,8 mol/l hydrochloric acid. Add 150 ml of hydrochloric acid (6.3) and 56 ml nitric acid (6.2) to 800 ml of water (6.1). 6.5 Standard stock solutions, boron, cobalt, copper, iron, manganese, molybdenum and zinc standard stock solutions,
= 1 000 mg/l for each element. Both single-element stock solutions and multi-element stock solutions with adequate specification, stating the acid used and the preparation technique, are commercially available. Multi-element stock solutions are usually available at the individual mass concentration
= 100 mg/l for each element. These solutions are considered to be stable for more than one year, but in reference to guaranteed stability, the recommendations of the manufacturer should be considered. Alternatively, the stock solutions may be prepared by dissolution of high purity metals. 6.5.1 Standard solution,
= 100 mg/l of element. Use commercially available multi-element solution of this concentration for each element or pipette 10 ml of the appropriate element stock solution (6.5) into a 100 ml volumetric flask. Add 10 ml of diluted nitric acid (6.2.1), fill to the mark with water and mix well. This solution is used to prepare spiked test solutions and calibration solutions. 6.6 Argon, purity 99,95 % or better. 7 Apparatus 7.1 Common laboratory glassware. 7.2 Inductively coupled plasma optical emission spectrometer. WARNING — It is essential that the manufacturer’s safety instructions are strictly observed when using this apparatus. The ICP atomic-emission spectrometer consists of a sample introduction system, the plasma as an excitation source, an optical system, a detector and a computer with suitable software. The sample is transported by the introduction system (rotation tube pump, nebulizer and a spray chamber) to the plasma torch. Around the torch a water cooled RF coil is placed. A frequency from 27 MHz to 56 MHz SIST EN 16963:2018
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