SIST EN 117:2005

Wood preservatives - Determination of toxic values against Reticulitermes species (European termites) (Laboratory method)

This document specifies a method for the determination of the toxic values of a wood preservative against the Reticulitermes species of European termites ).
This method is applicable to:
-   water-insoluble chemicals which are being studied as active insecticides;
-   organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; and
-   water soluble materials, for example salts.
NOTE   This method can be used in conjunction with an ageing procedure, for example EN 73.

Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenüber Reticulitermes-Arten (Europäische Termiten) (Laboratoriumsverfahren)

Dieses Dokument legt ein Verfahren zur Bestimmung der Grenze der Wirksamkeit eines Holzschutzmittels gegenüber Reticulitermes-Arten von europäischen Termiten ) fest.
Das Verfahren gilt für:
-   wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
-   organische, in Wasser dispergierbare Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; und
-   wasserlösliche Stoffe, zum Beispiel Salze.
ANMERKUNG   Das Verfahren kann in Verbindung mit einer Alterungsbeanspruchung, zum Beispiel nach EN 73, angewendet werden.

Produit de préservation du bois - Détermination du seuil d'efficacité contre les especes Reticulitermes (termites européens) (Méthode de laboratoire)

Le présente document décrit une méthode de détermination du seuil d'efficacité d'un produit de préservation du bois contre les especes de termites européens du genre Reticulitermes  ).
Cette méthode s'applique :
-   aux produits chimiques non hydrosolubles étudiés en tant que matieres actives ;
-   aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés ; et
-   aux produits hydrosolubles, par exemples des sels.
NOTE   Cette méthode peut s'appliquer conjointement a une épreuve de vieillissement, par exemple l'EN 73 ou l'EN 84.

Zaščitna sredstva za les – Ugotavljanje toksičnih vrednosti proti Reticulitermes santonensis (evropskim termitom) (Laboratorijska metoda)

General Information

• Status: Withdrawn
• Publication Date: 30-Jun-2005
• Withdrawal Date: 30-Dec-2012
• ICS: 71.100.50 - Wood-protecting chemicals
• Technical Committee: LLZ - Les, lesni izdelki in zaščita lesa
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 15-Nov-2012
• Due Date: 08-Dec-2012
• Completion Date: 31-Dec-2012
• Directive: 89/106/EEC - Construction products
• Ref Project: EN 117:2005 - Wood preservatives - Determination of toxic values against Reticulitermes species (European termites) (Laboratory method)

Relations

• Replaced By: SIST EN 117:2013 - Wood preservatives - Determination of toxic values against Reticulitermes species (European termites) (Laboratory method)
• Effective Date: 01-Jan-2013
• Replaces: SIST EN 117:1996 - Wood preservatives - Determination of toxic values against Reticulitermes santonensis de Feytaud (Laboratory method)
• Effective Date: 01-Jul-2005
• Replaces: SIST EN 117:1996/AC1:2002 - Wood preservatives - Determination of toxic values against Reticulitermes santonensis de Feytaud (Laboratory method)
• Effective Date: 01-Jul-2005

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 117:2005
01-julij-2005
1DGRPHãþD
SIST EN 117:1996
SIST EN 117:1996/AC1:2002
=DãþLWQDVUHGVWYD]DOHV±8JRWDYOMDQMHWRNVLþQLKYUHGQRVWLSURWL5HWLFXOLWHUPHV
VDQWRQHQVLVHYURSVNLPWHUPLWRP/DERUDWRULMVNDPHWRGD
Wood preservatives - Determination of toxic values against Reticulitermes species
(European termites) (Laboratory method)
Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenüber Reticulitermes-
Arten (Europäische Termiten) (Laboratoriumsverfahren)
Produit de préservation du bois - Détermination du seuil d'efficacité contre les especes
Reticulitermes (termites européens) (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 117:2005
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
SIST EN 117:2005 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 117:2005

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SIST EN 117:2005



EUROPEAN STANDARD
EN 117

NORME EUROPÉENNE

EUROPÄISCHE NORM
March 2005
ICS 71.100.50 Supersedes EN 117:1989
English version
Wood preservatives - Determination of toxic values against
Reticulitermes species (European termites) (Laboratory method)
Produit de préservation des bois - Détermination des Holzschutzmittel - Bestimmung der Grenze der
valeurs toxiques contre les espèces Reticulitermes Wirksamkeit gegenüber Recticulitermes-Arten
(termites européens) (Méthode de laboratoire) (europäische Termiten) (Laborverfahren)
This European Standard was approved by CEN on 3 February 2005.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.

CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.




EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 117:2005: E
worldwide for CEN national Members.

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SIST EN 117:2005
EN 117:2005 (E)
Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Normative reference .5
3 Terms and definitions .5
4 Principle.5
5 Test materials.6
6 Sampling.8
7 Test specimens.8
8 Procedure .9
9 Expression of results .16
10 Test report .16
Annex A (informative) Example of a test report .18
Annex B (informative) Example of a method of culturing termites .20
Annex C (informative) Environmental, health and safety precautions within chemical/biological
laboratory .21
Bibliography .22

2

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SIST EN 117:2005
EN 117:2005 (E)
Foreword
This document (EN 117:2005) has been prepared by Technical Committee CEN/TC 38 “Durability of wood
and wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by September 2005, and conflicting national standards shall be
withdrawn at the latest by September 2005.
This document supersedes EN 117:1989.
Significant technical differences between this document and EN 117:1989 are as follows:
a) introduction of new harmonised specifications for the test specimens used in the diverse biological tests;
b) acknowledgment of the terms given in EN 1001-1;
c) introduction of an informative Annex to take account of consideration for minimisation of environmental
and health hazards caused by the use of this biological test.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland
and United Kingdom.

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SIST EN 117:2005
EN 117:2005 (E)

Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the
effectiveness of a wood preservative against the Reticulitermes species of European termites. It allows the
determination of the concentration at which the product completely prevents attack by these insects of
impregnated wood of a susceptible species.
This laboratory method provides one criterion by which the value of a product can be assessed. In making this
assessment the methods by which the preservative can be applied should be taken into account. It is further
recommended that results from this should be supplemented by those from other appropriate tests, and above
all by comparison with practical experience.
When products which are very active at low concentrations are used it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other products,
treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate
rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see
also Annex C for environmental, health and safety precautions).

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SIST EN 117:2005
EN 117:2005 (E)
1 Scope
This document specifies a method for the determination of the toxic values of a wood preservative against the
1)
Reticulitermes species of European termites .
This method is applicable to:
 water-insoluble chemicals which are being studied as active insecticides;
 organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates; and
 water-soluble materials, for example salts.
NOTE This method can be used in conjunction with an ageing procedure, for example EN 73 or EN 84.
2 Normative reference
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the
total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
Impregnation of several sets of test specimens of susceptible wood with a series of solutions in which the
concentration of preservative is ranged in a given progression.

1) The method can be applied not only to different species of Reticulitermes, but also to other species of the family of the
Rhinotermitidae, adapting the conditions of temperature and humidity where necessary to the specific requirements of the
species concerned.

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EN 117:2005 (E)

2)
Exposure of these test specimens to specified colonies of Reticulitermes and assessment of the attack
suffered after exposure under fixed conditions and over a fixed period.
Comparison of these results with those obtained with untreated and solvent or diluent-treated control test
specimens.
Derivation of the toxic values of the product under test.
5 Test materials
5.1 Biological material
Workers, soldiers and nymphs of an identified termite species of Reticulitermes.
NOTE 1 The termite species and the locality of origin should be stated in the test report and their identification should
be proved.
NOTE 2 The termites should be obtained from colonies reared as described in Annex B.
5.2 Products and reagents
5.2.1 Substrate for establishing the colonies. A choice of:
5.2.1.1 Fine white quartz sand consisting of grains of crystallized silica, very pure (99,5 % silica), and free
3)
from any organic substances .
5.2.1.2 An hydrated, laminar, aluminium-iron-magnesium silicate exfoliated to give particles of 1 mm
3 3
to 3 mm with an apparent density of 80 kg/m to 90 kg/m . Particles of less than 1 mm shall be eliminated by
sieving prior to use to ensure the absence of free water and prevent any significant agglomeration of the
particles.
3
5.2.1.3 Rigid polyurethane foam with open pores of mass per unit of volume of 14 kg/m and

4) 2 2
compressive strength of 0,02 N/mm to 0,03 N/mm . It is advisable to cut the foam into sheets 15 mm thick.
5.2.2 Fumigant (if necessary) xylene, technical grade, mixed isomers.
5.2.3 Water, complying with grade 3 of EN ISO 3696.
5.2.4 Solvent or diluent, a suitable volatile liquid that will dissolve or dilute the preservative but does not
leave a residue in the wood which would have a toxic effect on the insect at the end of the conditioning period.
5.2.5 Filter paper, ordinary quality, medium-fast grade.

2) In providing biological validation of individual species, it is essential that the locality of origin of each test termite
species is given. The description of the locality should at least include the district name.
3) In France Fontainebleau sand, of which more than 97 % of the particles are between 75 µm and 300 µm in size,
provides these features.
4) Determined in accordance with IS0 844.
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EN 117:2005 (E)
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (26 ± 2) °C and at a minimum relative humidity
of (70 ± 5) %.
5)
5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and relative humidity (65 ± 5) % .
6
5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out .
5.3.4 Testing chamber, protected from light, ventilated and controlled at (26 ± 2) °C and at a minimum
relative humidity of (70 ± 5) %.
5.3.5 Treatment vessels, of a material that does not react with the preservative under test, for example of
glass for organic products and of polyethylene for salts containing fluorine.
5.3.6 Drying vessel(s), capable of holding sets of three test specimens (7.5), provided with a close-fitting
cover and containing supports that will give minimum contact with treated test specimens to be placed on
them. The vessels and supports shall be of a material that does not react with the preservative under test, for
example glass for organic compounds and polyethylene for products containing fluorine.
5.3.7 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with
which they come in contact during the test.
5.3.8 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to
ensure the safety of the operator.
5.3.9 Vacuum vessels, fitted with stopcocks, capable of receiving the treatment.
5.3.10 Vacuum pump fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa.
5.3.11 Instruments adapted for termite manipulation (aspirator, forceps).
5.3.12 Test containers, suitable for holding the test specimens and of material resistant to the product used,
and fitted with perforated cover to provide a good exchange of air.
2 2
Base area 35 cm to 60 cm .
Minimum height 8,5 cm.
3 3
Volume 500 cm to 1 000 cm .
5.3.13 Glass rings 20 mm high, 20 mm in diameter and with a wall thickness of at least 1 mm.
5.3.14 Protective gloves
5.3.15 Ordinary laboratory equipment including a balance capable of weighing to an accuracy of 0,01 g.

5) The conditioning of test specimens after treatment can be carried out in the laboratory work area (5.3.3) provided that
this meets the conditions specified for the conditioning chamber (5.3.2).
6) It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of
operators to solvents or their vapours.

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6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples should be stored and
handled in accordance with any written recommendations from the supplier.
NOTE For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The reference species is Scots pine (Pinus sylvestris Linnaeus).
NOTE Additional tests can be carried out using other species but, if so, this should be stated in the test report.
7.2 Wood quality
The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not
have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees
preferably felled in winter.
NOTE 1 Wood that has been kiln dried at temperatures below 60 °C can be used.
The wood shall be exclusively sapwood containing little resin and having between 2,5 annual rings per 10 mm
and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the
whole.
NOTE 2 It is recommended to use test specimens of similar growth rate within a single test.
7.3 Provision of test specimens
Prepare planed strips having a cross-section of (25 ± 0,5) mm x (15 ± 0,5) mm removing a minimum of 2 mm
from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction of the grain.
The annual rings shall have a contact angle of greater than 10° to the broad faces of the test specimens.
Make transverse cuts, neatly to give sharp edges and a fine-sawn finish to the end grain surfaces, to give test
specimens (50 ± 0,5) mm long.
The test specimens shall originate from a minimum of three trees or shall be taken at random from a stock
originally of more than 500 test specimens.
7.4 Dimensions of test specimens
The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2) shall be
(50 ± 0,5) mm x (25 ± 0,5) mm x (15 ± 0,5) mm.
For the purposes of calculating the mass of preservative retained per unit volume of wood (8.1.2.2) the
3
nominal volume of each test specimen shall be taken as 18,75 cm .
Mark each test specimen so that it can be identified throughout the test.
7.5 Number and distribution of test specimens
The test specimens shall be divided as follows:
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EN 117:2005 (E)
a) treated test specimens: these are the test specimens which are impregnated and subject to attack by
Reticulitermes, use at least three test specimens for each concentration of the product;
b) untreated control test specimens for checking the virulence of the termite taken for the test: these non-
impregnated test specimens are subjected to attack by Reticulitermes; they are three in number;
c) solvent or diluent treated control test specimens subjected to attack by Reticulitermes; they are three in
number.
8 Procedure
8.1 Preparation of test specimens
8.1.1 Conditioning of test specimens prior to treatment
Allow the test specimens to condition in the conditioning chamber (5.3.2) for a minimum of two weeks.
8.1.2 Treatment of test specimens
8.1.2.1 Preparation of treatment solutions
8.1.2.1.1 Solid preservatives
 Water-soluble preservatives: dissolve the preservative in water (5.2.3) to the required concentration.
 Non-water-soluble preservatives: dissolve the preservative in an appropriate solvent (5.2.4) to the
required concentration.
8.1.2.1.2 Liquid preservatives
If appropriate, use the preservative without further preparation other than any necessary stirring. If it is a
concentrate, dilute the preservative with the diluent to the required working concentration, using the procedure
specified by the supplier.
Prepare a series of at least 5 concentrations by mass, distributed evenly about the expected toxic values. A
solvent or diluent control, i.e. treatment at concentration = 0, shall also be used. If the approximate toxic
values are unknown, the concentrations shall form a widely spaced geometric progression for a first test and a
more closely spaced geometric or arithmetic progression for subsequent tests.
All treatment solutions shall be freshly prepared.
8.1.2.2 Impregnation
Carry out impregnation in ascending order of concentration, starting with the solvent control (concentration =
0). The following procedure ensures the required complete impregnation of test specimens by the test
solutions. For each concentration weigh each test specimen to the nearest 0,05 g, and then stack the test
specimens in one of the treatment vessels (5.3.5) so that as much of their face as possible is exposed (e.g. by
piling them crosswise). Ballast the stack of test specimens with weights (5.3.7) to prevent them from floating
later when the liquid is admitted.
Place each treatment vessel in one of the vacuum vessels (5.3.9), and reduce the pressure to 700 Pa.
Maintain this for 15 min. Observe the proper safety measures for vacuum vessels. After this period, close the
stopcock to the vacuum pump (5.3.10) and open the other stopcock to allow the solution of preservative to be
drawn into the treatment vessel. Keep the test specimens covered completely by the solution throughout the
remainder of the impregnation process.
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EN 117:2005 (E)
Next, admit air to bring the vacuum vessel back to atmospheric pressure, remove the treatment vessel with its
submerged test specimens from the vacuum vessel, cover it and leave it for 2 h, adding further solution if
necessary to keep the test specimens fully covered by the liquid.
After this impregnation treatment remove the test specimens one by one, remove the excess liquid from their
faces by lightly blotting with filter paper (5.2.5), and immediately weigh to the nearest 0,05 g.
In the case of water-soluble preservatives, for example salts and organic chemicals which are being studied
as active substances, calculate the mass of active matter retained by each test specimen from the mass of
7)
solution absorbed and its concentration.
In the case of organic formulations and organic water-dispersible formulations the retention is expressed for
each test specimen in terms of the corresponding mass of the formulation retained but, if a concentration is
supplied, the retention is expressed in terms of the solution prepared ready for use as specified by the
supplier.
Calculate the mass of preservative retained per unit volume of wood in kilograms per cubic metr
...