SIST EN ISO 23674:2023
(Main)Cosmetics - Analytical methods - Direct determination of traces of mercury in cosmetics by thermal decomposition and atomic absorption spectrometry (mercury analyser) (ISO 23674:2022)
Cosmetics - Analytical methods - Direct determination of traces of mercury in cosmetics by thermal decomposition and atomic absorption spectrometry (mercury analyser) (ISO 23674:2022)
This document specifies an analytical procedure for direct determination of traces of mercury in finished cosmetic products by thermal decomposition – atomic absorption spectrometry (mercury analyser).
This document aims to provide a procedure of quantification of mercury traces in cosmetic products that consumers can be exposed to in their usage. This method describes the determination of mercury traces in cosmetics by direct solid analysis with no need of prior digestion. Total mercury (both inorganic and organic species) can be quantified either in solid or liquid samples.
Kosmetische Mittel - Untersuchungsverfahren - Direkte Bestimmung von Quecksilberspuren in kosmetischen Mitteln mittels thermischer Zersetzung und Atomabsorptionsspektrometrie (Quecksilber-Analysator) (ISO 23674:2022)
Dieses Dokument legt ein Untersuchungsverfahren zur direkten Bestimmung von Quecksilberspuren in kosmetischen Endprodukten mittels thermischer Zersetzung Atomabsorptionsspektrometrie (Quecksilber-Analysator) fest.
Dieses Dokument zielt darauf ab, ein Verfahren zur Quantifizierung von Quecksilberspuren in kosmetischen Mitteln bereitzustellen, denen Verbraucher bei ihrer Verwendung ausgesetzt sein können. Dieses Verfahren beschreibt die Bestimmung von Quecksilberspuren in kosmetischen Mitteln durch direkte Feststoffanalyse, ohne die Notwendigkeit eines vorherigen Aufschlusses. Das gesamte Quecksilber (sowohl anorganische als auch organische Spezies) kann entweder in festen oder flüssigen Proben quantifiziert werden.
Cosmétiques - Méthodes d’analyse - Dosage direct des traces de mercure dans les cosmétiques par décomposition thermique et spectrométrie d’absorption atomique (analyseur de mercure) (ISO 23674:2022)
Le présent document spécifie un mode opératoire d’analyse pour le dosage direct des traces de mercure dans les produits cosmétiques finis par décomposition thermique – spectrométrie d’absorption atomique (analyseur de mercure).
Le présent document a pour but de fournir un mode opératoire pour la quantification des traces de mercure dans les produits cosmétiques auxquelles les consommateurs sont susceptibles d’être exposés lors de leur utilisation. Cette méthode décrit le dosage des traces de mercure dans les cosmétiques par analyse solide directe sans nécessiter de digestion préalable. Le mercure total (à la fois les espèces inorganiques et organiques) peut être quantifié aussi bien dans des échantillons solides que liquides.
Kozmetika - Analizne metode - Neposredno določevanje živega srebra v sledovih v kozmetičnih izdelkih s termično razgradnjo in atomsko absorpcijsko spektrometrijo (analizator živega srebra) (ISO 23674:2022)
Ta mednarodni standard določa način določanja živega srebra v kozmetičnih izdelkih z integriranimi analiznimi sistemi za živo srebro. Cilj tega standarda je:
• Opis analitičnega postopka
• Validacija in karakterizacija metode s profilom točnosti
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-april-2023
Kozmetika - Analizne metode - Neposredno določevanje živega srebra v sledovih v
kozmetičnih izdelkih s termično razgradnjo in atomsko absorpcijsko
spektrometrijo (analizator živega srebra) (ISO 23674:2022)
Cosmetics - Analytical methods - Direct determination of traces of mercury in cosmetics
by thermal decomposition and atomic absorption spectrometry (mercury analyser) (ISO
23674:2022)
Kosmetische Mittel - Untersuchungsverfahren - Direkte Bestimmung von
Quecksilberspuren in kosmetischen Mitteln mittels thermischer Zersetzung und
Atomabsorptionsspektrometrie (Quecksilber-Analysator) (ISO 23674:2022)
Cosmétiques - Méthodes d’analyse - Dosage direct des traces de mercure dans les
cosmétiques par décomposition thermique et spectrométrie d’absorption atomique
(analyseur de mercure) (ISO 23674:2022)
Ta slovenski standard je istoveten z: EN ISO 23674:2022
ICS:
71.100.70 Kozmetika. Toaletni Cosmetics. Toiletries
pripomočki
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 23674
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2022
EUROPÄISCHE NORM
ICS 71.100.70
English Version
Cosmetics - Analytical methods - Direct determination of
traces of mercury in cosmetics by thermal decomposition
and atomic absorption spectrometry (mercury analyser)
(ISO 23674:2022)
Cosmétiques - Méthodes d'analyse - Dosage direct des Kosmetische Mittel - Untersuchungsverfahren - Direkte
traces de mercure dans les cosmétiques par Bestimmung von Quecksilberspuren in kosmetischen
décomposition thermique et spectrométrie Mitteln mittels thermischer Zersetzung und
d'absorption atomique (analyseur de mercure) (ISO Atomabsorptionsspektrometrie (Quecksilber-
23674:2022) Analysator) (ISO 23674:2022)
This European Standard was approved by CEN on 15 August 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23674:2022 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 23674:2022) has been prepared by Technical Committee ISO/TC 217
"Cosmetics" in collaboration with Technical Committee CEN/TC 392 “Cosmetics” the secretariat of
which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2023, and conflicting national standards shall
be withdrawn at the latest by March 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 23674:2022 has been approved by CEN as EN ISO 23674:2022 without any modification.
INTERNATIONAL ISO
STANDARD 23674
First edition
2022-08
Cosmetics — Analytical methods —
Direct determination of traces of
mercury in cosmetics by thermal
decomposition and atomic absorption
spectrometry (mercury analyser)
Cosmétiques — Méthodes d’analyse — Dosage direct des traces
de mercure dans les cosmétiques par décomposition thermique et
spectrométrie d’absorption atomique (analyseur de mercure)
Reference number
ISO 23674:2022(E)
ISO 23674:2022(E)
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 23674:2022(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus and equipment . 2
7 Calibration .3
7.1 General . 3
7.2 Liquid calibration standards. 3
7.3 Solid calibration standards . 4
8 Procedure .5
8.1 General . 5
8.2 Preparation of samples . 5
8.2.1 General recommendations . 5
8.2.2 General of samples . 5
9 Instrument parameters . 5
10 Quality control of the analysis . 5
10.1 General . 5
10.2 Quality control procedure . 5
10.2.1 Analysis blanks . 5
10.2.2 Quality control samples . 6
10.2.3 Replicates . 6
11 Calculation . 6
12 Method performance .6
13 Test report . 7
Annex A (informative) Performance of the method determined by the accuracy profile
methodology . 8
Annex B (informative) ISO 23674 and ISO 23821 common ring test results .13
Bibliography .16
iii
ISO 23674:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 217, Cosmetics, in collaboration with
the European Committee for Standardization (CEN) Technical Committee CEN/TC 392, Cosmetics, in
accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
ISO 23674:2022(E)
Introduction
[1]
This document has been developed in parallel with ISO 23821 . Knowing this, an interlaboratory test
using either one or the other method was performed on same tailor-made cosmetic products in order to
establish that both methods fulfilled the same requirements (see Annex B).
v
INTERNATIONAL STANDARD ISO 23674:2022(E)
Cosmetics — Analytical methods — Direct determination of
traces of mercury in cosmetics by thermal decomposition
and atomic absorption spectrometry (mercury analyser)
1 Scope
This document specifies an analytical procedure for direct determination of traces of mercury in
finished cosmetic products by thermal decomposition – atomic absorption spectrometry (mercury
analyser).
This document aims to provide a procedure of quantification of mercury traces in cosmetic products
that consumers can be exposed to in their usage. This method describes the determination of mercury
traces in cosmetics by direct solid analysis with no need of prior digestion. Total mercury (both
inorganic and organic species) can be quantified either in solid or liquid samples.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
validation range
range from the upper to the lower concentration of samples used for the method evaluation
3.2
validated range
range of concentrations between the upper and lower levels that the method performance has been
demonstrated to be compliant with the method requirements
Note 1 to entry: The validated range shall not be confused with the validation range (3.1); it can be smaller.
4 Principle
The described method uses integrated instruments allowing mercury traces quantification. Samples
are weighed with no need of any chemical sample preparation as they are thermally decomposed in the
instrument (burned or ashed) in an oxygen flow at high temperature (between 650 °C and 900 °C). The
combustion gases travel through a catalyst tube set at about 615 °C. This step ensures conversion of
interfering components to forms that do not interfere and that are subsequently flushed. The resulting
mercury vapour is enriched on a downstream gold amalgamator and is then released as atomic
vapour by rapid heating of the amalgamator at a temperature of 800 °C to 900 °C. The atomic vapour
ISO 23674:2022(E)
is passed through a measuring cuvette system. The quantification occurs thanks to absorption at
253,7 nm. A wide dynamic range may be achieved by simultaneously passing mercury vapours through
measurement cells of different lengths.
5 Reagents
WARNING — The use of this document can involve hazardous materials, operations and
equipment. This document does not address all the safety risks associated with its use. It is the
responsibility of the analyst to take all appropriate measures for ensuring the safety and health
of the personnel prior to application of the document.
-1
5.1 Water, conforming to Grade 1 of ISO 3696 (conductivity below 0,1 μS.cm at 25 °C).
5.2 Hydrochloric acid, minimum mass fraction w = 30 %, density = 1,15 g/ml, suitable for elemental
analysis.
5.3 Diluted hydrochloric acid, produced by mixing hydrochloric acid (5.2) with ultrapure water
(5.1) at a ratio of approximately 1+9 parts, respectively.
5.4 Diluted nitric acid, prepared by diluting nitric acid of minimum mass fraction w=60 %,
density = 1,15 g/ml, suitable for ICP-MS with pure water (5.1) at a ratio of 1+9 respectively.
[2]
If diluted nitric acid is chosen to dilute the analyte stock solutions, it is recommended to add
[3]
L-cysteine at 0,1 g/l .
5.5 Analyte standard stock solutions (mercury), 1 000 µg/ml (commercially available).
5.6 Analyte standard stock solutions (mercury), 10 µg/ml [commercially available or freshly
prepared by dilution in the same dilution medium as calibration solutions (5.3 or 5.4) of a more
concentrated solution for example at 1 000 µg/ml such as 5.5].
Analyte standard stock solutions 5.5 or 5.6 can be used for this document according to their availability
on the local market. Recommendations from the supplier of stock solutions regarding stability (expiry
date and storage conditions) shall be followed to avoid mercury loss.
6 Apparatus and equipment
All apparatus and equipment that come into direct contact with samples or solutions should be pre-
cleaned with diluted hydrochloric acid (5.3) and rinsed with ultrapure water (5.1) to ensure the lowest
analytical background. To prevent contamination and adsorption, do not use laboratory materials made
up of borosilicate glass. The use of diluted nitric acid (5.4) is also permitted. To check the efficiency of
the cleaning step, a blank measurement can be performed prior to the analytical sequence.
Elemental mercury analyser with instrument control unit and sample containers made of suitable
materials (e.g. nickel, quartz glass or ceramic) are used. The sample containers usually have a capacity
of approximately 500 mg of solid sample or 500 µl, 1 000 µl and 1 500 µl of liquids. Mercury analysers
are equipped with an element-specific lamp for mercury. The mercury line of 253,7 nm is used.
Many instruments from several brands are available on the market and often marketed as “mercury
analysers”. The list of instruments that have been used for the interlaboratory test validating this
document is given in Annex A.
ISO 23674:2022(E)
7 Calibration
7.1 General
The aim of this step is to build a calibration curve by introduction in the instrument of increasing
mercury amounts. This calibration curve allows to get instrument response as a function of mercury
amount (in ng). At least 5 calibration standards shall be used in a range including the expected amounts
of mercury in the samples. Calibration of the instrument remains stable and is not mandatory prior to
each series of analyses provided that quality control (QC) requirements are met (see 10.2).
Due to the specificity of the technique towards mercury element, measurement of traces of mercury
in samples is weakly affected by interferences and matrix effects. Since the technique is relatively
insensitive to the matrix type, calibration can be performed either using in-house liquid (see 7.2) or solid
(see 7.3) calibration standards. However, moisture and organic contents may affect quantification. Such
differences between calibration standard and cosmetic samples shall be neutralized. For differences
in moisture content, optimize the drying times. For differences in quantity of organic material to be
combusted, optimize the time and temperature of combustion. For differences in interfering element,
optimize time for the catalytic conversion of elements needing catalytic conversion into a chemical
form that does not interfere with mercury detection.
7.2 Liquid calibration standards
Calibration solutions should be prepared in either diluted hydrochloric acid (5.3) or diluted nitric acid
containing L-Cysteine (5.4) to ensure stability of mercury. For laboratory convenience, calibration
solutions may be prepared in other acid mixture solutions, provided the operator checks the stability
of the mercury in that solution. Fresh calibration solutions should be prepared each time calibration is
needed. Two solution calibration procedures are possible.
— Introduce increasing amounts of mercury in a constant volume using increasing concentrations of
standard solution (see Table 1).
— Introduce increasing amounts of mercury using increasing volumes of one or more standard
solutions with a given mercury concentration (see Table 2).
Below are examples for these two ways of performing calibration.
Table 1 — Example of calibration solutions using constant volumes of different standards
Mercury concen- Mercury
Part of 10 µg/g
...
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