SIST-TS CEN/TS 17701-2:2023
(Main)Plant biostimulants - Determination of specific elements - Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn
Plant biostimulants - Determination of specific elements - Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn
This document specifies a method for the determination of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in plant biostimulant digests using inductively coupled plasma-atomic emission spectrometry (ICP-AES).
This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend applies.
This method is applicable to aqua regia digests prepared according to CEN/TS 17701-1. The method can be used for the determination of other elements, provided the user has verified the applicability.
Pflanzen-Biostimulanzien - Bestimmung spezifischer Elemente - Teil 2: Bestimmung des Gesamtgehaltes an Cd, Pb, Ni, As, Cr, Cu und Zn
Dieses Dokument legt ein Verfahren zur Bestimmung des Gehalts an Arsen (As), Cadmium (Cd), Kupfer (Cu), Chrom (Cr), Blei (Pb), Nickel (Ni) und Zink (Zn) in Biostimulanzien für die pflanzliche Anwendung mit Atomemissionsspektrometrie mit induktiv gekoppeltem Plasma (ICP AES) nach Aufschluss fest.
Dieses Dokument gilt auch für die Mischungen von Düngeprodukten, in denen die Biostimulanzien für die pflanzliche Anwendung der Hauptbestandteil der Mischung sind. Andernfalls gilt die Technische Spezifikation für den Hauptbestandteil der Mischung.
Das Verfahren gilt für Königswasseraufschlüsse, die nach CEN/TS 17701-1 hergestellt wurden. Das Verfahren kann zur Bestimmung anderer Elemente angewendet werden, vorausgesetzt der Anwender hat die Anwendbarkeit überprüft.
Biostimulants des végétaux - Dosage des éléments spécifiques - Partie 2 : Détermination de la teneur totale en Cd, Pb, Ni, As, Cr, Cu et Zn
Le présent document spécifie une méthode pour le dosage de l’arsenic (As), du cadmium (Cd), du cuivre (Cu), du chrome (Cr), du plomb (Pb), du nickel (Ni) et du zinc (Zn) dans les digestats de biostimulants des végétaux par spectrométrie d’émission atomique par un plasma à couplage inductif (ICP-AES).
Le présent document est également applicable aux mélanges de fertilisants dont la majeure partie est constituée de biostimulants des végétaux. Dans le cas contraire, la Spécification technique relative au constituant principal du mélange s’applique.
Cette méthode est applicable aux digestats à l’eau régale préparés conformément à la CEN/TS 17701-1. La méthode peut également être utilisée pour le dosage d’autres éléments, à condition que l’utilisateur ait vérifié son applicabilité.
Rastlinski biostimulanti - Določevanje specifičnih elementov - 2. del: Določevanje celotnega Cd, Pb, Ni, As, Cr, Cu in Zn
Ta dokument določa metodo za določanje arzena (As), kadmija (Cd), bakra (Cu), kroma (Cr), svinca (Pb), niklja (Ni) in cinka (Zn) v rastlinskih biostimulantnih presnovkih z atomsko emisijsko spektrometrijo z induktivno sklopljeno plazmo (ICP-AES).
Dokument je uporaben tudi za mešanice sredstev za gnojenje, pri katerih so rastlinski biostimulanti glavni del mešanice. V nasprotnem primeru se uporablja tehnična specifikacija za glavni del mešanice.
Metoda se uporablja za izlužke zlatotopke, pripravljene v skladu s standardom CEN/TS 17701-1. Metodo je mogoče uporabiti za določanje drugih elementov, če uporabnik preveri uporabnost.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST-TS CEN/TS 17701-2:2023
01-februar-2023
Rastlinski biostimulanti - Določevanje specifičnih elementov - 2. del: Določevanje
celotnega Cd, Pb, Ni, As, Cr, Cu in Zn
Plant biostimulants - Determination of specific elements - Part 2: Determination of total
content of Cd, Pb, Ni, As, Cr, Cu and Zn
Pflanzen-Biostimulanzien - Bestimmung spezifischer Elemente - Teil 2: Bestimmung des
Gesamtgehaltes an Cd, Pb, Ni, As, Cr, Cu und Zn
Biostimulants des végétaux - Dosage des éléments spécifiques - Partie 2 :
Détermination de la teneur totale en Cd, Pb, Ni, As, Cr, Cu et Zn
Ta slovenski standard je istoveten z: CEN/TS 17701-2:2022
ICS:
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17701-2:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
SIST-TS CEN/TS 17701-2:2023
---------------------- Page: 2 ----------------------
SIST-TS CEN/TS 17701-2:2023
CEN/TS 17701-2
TECHNICAL SPECIFICATION
SPÉCIFICATION TECHNIQUE
March 2022
TECHNISCHE SPEZIFIKATION
ICS 65.080
English Version
Plant biostimulants - Determination of specific elements -
Part 2: Determination of total content of Cd, Pb, Ni, As, Cr,
Cu and Zn
Biostimulants des végétaux - Dosage des éléments Biostimulanzien für die pflanzliche Anwendung -
spécifiques - Partie 2 : Détermination de la teneur Bestimmung spezifischer Elemente - Teil 2:
totale en Cd, Pb, Ni, As, Cr, Cu et Zn Bestimmung des Gesamtgehaltes an Cd, Pb, Ni, As, Cr,
Cu und Zn
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17701-2:2022 E
worldwide for CEN national Members.
---------------------- Page: 3 ----------------------
SIST-TS CEN/TS 17701-2:2023
CEN/TS 17701-2:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Interferences . 6
5.1 General . 6
5.2 Spectral interferences . 6
5.3 Transport interferences . 6
5.4 Excitation interferences . 6
5.5 Chemical interferences . 7
5.6 Memory interferences . 7
6 Reagents . 7
7 Apparatus . 8
7.1 Common laboratory glassware. . 8
7.2 Inductively coupled plasma atomic emission spectrometer. . 8
8 Procedure. 8
8.1 Preparation of test and blank solution . 8
8.2 Preparation of the calibration solutions . 8
8.3 Measurement . 9
8.3.1 Instrument conditions . 9
8.3.2 Optimization of the instrument conditions . 9
8.3.3 Interferences . 9
8.3.4 Measurement . 9
8.3.5 Matrix effects . 9
8.3.6 Spiking. 10
9 Calculation and expression of the results . 11
10 Test report . 12
Bibliography . 13
2
---------------------- Page: 4 ----------------------
SIST-TS CEN/TS 17701-2:2023
CEN/TS 17701-2:2022 (E)
European foreword
This document (CEN/TS 17701-2:2022) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association.
The CEN/TS 17701 series, Plant biostimulants - Determination of specific elements, consists of the
following parts:
— Part 1: Digestion by aqua regia for subsequent determination of elements;
— Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn;
— Part 3: Determination of mercury.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN/CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
3
---------------------- Page: 5 ----------------------
SIST-TS CEN/TS 17701-2:2023
CEN/TS 17701-2:2022 (E)
Introduction
This document was prepared by the experts of CEN/TC 455 “Plant Biostimulants”. The European
Committee for Standardization (CEN) was requested by the European Commission (EC) to draft
European standards or European standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down
rules on the making available on the market of EU fertilising products (“FPR” or “Fertilising Products
Regulation”).
This standardization request, presented as M/564, also contributes to the Communication on “Innovating
for Sustainable Growth: A Bio economy for Europe”. Working Group 4 “Other safety parameters” was
created to develop a work program as part of this standardization request. Technical Committee CEN/TC
455 “Plant Biostimulants” was established to carry out the work program that will prepare a series of
standards. The interest in biostimulants has increased significantly in Europe as a valuable tool to use in
agriculture. Standardization was identified as having an important role in order to promote the use of
biostimulants. The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby
improving the confidence of farmers, industry, and consumers in biostimulants, and will promote and
support commercialisation of the European biostimulant industry.
The preparation of this document is based on a standardization request to CEN by the European
Commission and the European Free Trade Association (Mandate M/564) concerning the modernization
of methods of analysis of fertilizers in the framework of Regulation (EU) 2019/1009 of the European
Parliament and of the Council.
This document is part of a modular approach and concerns the analytical measurement step. “Modular”
means that a test standard concerns a specific step in assessing a property and not the whole chain of
measurements. Inductively coupled plasma atomic emission spectrometry (ICP-AES) is nowadays widely
used and a well-established method in many laboratories.
WARNING — Persons using this document should be familiar with usual laboratory practice. This
document does not purport to address all of the safety issues, if any, associated with its use. It is the
responsibility of the user to establish appropriate health and safety practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this document are carried out
by suitably trained staff.
4
---------------------- Page: 6 ----------------------
SIST-TS CEN/TS 17701-2:2023
CEN/TS 17701-2:2022 (E)
1 Scope
This document specifies a method for the determination of arsenic (As), cadmium (Cd), copper (Cu),
chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in plant biostimulant digests using inductively coupled
plasma-atomic emission spectrometry (ICP-AES).
This document is also applicable to the blends of fertilizing products where plant biostimulants are the
main part of the blend. Otherwise, the Technical Specification for the main part of the blend applies.
This method is applicable to aqua regia digests prepared according to CEN/TS 17701-1. The method can
be used for the determination of other elements, provided the user has verified the applicability.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
CEN/TS 17701-1, Plant biostimulants - Determination of specific elements - Part 1: Digestion by aqua regia
for subsequent determination of elements
CEN/TS 17704, Plant biostimulants - Determination of dry matter
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
analyte
parameter to be determined
3.2
blank calibration solution
solution prepared in the same way as the calibration solution but leaving out the analytes
3.3
blank test solution
solution prepared in the same way as the test sample solution but omitting the test portion
3.4
calibration solution
solution used to calibrate the instrument, prepared from stock solutions by adding acids, buffer, reference
element and salts as needed
3.5
digest
solution received after mineralization of the organic matter of a sample and dissolution of its mineral
part, more or less completely, when reacting with a reagent mixture
3.6
stock solution
solution with accurately known analyte concentration(s), prepared from pure chemicals (6.5)
3.7
test sample solution
solution prepared after extraction or digestion of the test sample according to appropriate specifications
5
---------------------- Page: 7 ----------------------
SIST-TS CEN/TS 17701-2:2023
CEN/TS 17701-2:2022 (E)
4 Principle
The method is based on the inductively coupled plasma atomic emission spectrometry (ICP-AES)
measurement of the concentration of arsenic, cadmium, copper, chromium, lead, nickel and zinc in plant
biostimulant aqua regia digests. The elements are determined after an appropriate dilution of the digest,
if necessary. The solution is dispersed by a suitable nebulizer of the ICP-AES instrument and the resulting
aerosol is transported into the plasma torch. Element specific emission spectra are produced by a
radiofrequency inductively coupled argon plasma where atoms or ions are excited at high temperature.
The emission spectra are dispersed by a spectrometer, and the intensities of the emission lines are
monitored by photosensitive devices. Multi-element determinations using sequential or simultaneous
optical systems and axial, radial or dual viewing of the plasma may be used.
5 Interferences
5.1 General
Interferences and matrix effects shall be recognized and appropriate measures to minimize them shall be
made. There are several types of interferences – see 5.2 to 5.6.
5.2 Spectral interferences
Spectral interferences are due to incomplete isolation of the radiation emitted by the analyte from other
detected radiation sources. Spectral interferences are caused by the background emission from
continuous or recombination phenomena, by stray light which causes background increase or overlap of
a spectral line from another element, or by the unresolved overlap of molecular band spectra. Background
emission and stray light can usually be compensated for by subtracting the background emission
measured adjacent to the analyte wavelength peak. To correct a sloping background shift background
correction points on each side of the peak are used. The increase of background is more intensive with
axial-view instruments. Background correction is not required in cases of line broadening where a
background correction measurement would actually degrade the analytical result. A spectral line overlap
usually leads to the choice of an alternative line. If this is not possible, mathematical correction
procedures (e.g. inter-element correction technique or
...
SLOVENSKI STANDARD
kSIST-TS FprCEN/TS 17701-2:2021
01-november-2021
Rastlinski biostimulansi - Določevanje določenih elementov - 2. del: Določevanje
celotnega Cd, Pb, Ni, As, Cr, Cu in Zn
Plant biostimulants - Determination of specific elements - Part 2: Determination of total
content of Cd, Pb, Ni, As, Cr, Cu and Zn
Biostimulanzien für die pflanzliche Anwendung - Bestimmung spezifischer Elemente -
Teil 2: Bestimmung des Gesamtgehaltes an Cd, Pb, Ni, As, Cr, Cu und Zn
Biostimulants des plantes - Détermination des éléments spécifiques - Partie 2 :
Détermination du contenu total en Cd, Pb, Ni, As, Cr, Cu et Zn
Ta slovenski standard je istoveten z: FprCEN/TS 17701-2
ICS:
65.080 Gnojila Fertilizers
kSIST-TS FprCEN/TS 17701-2:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
kSIST-TS FprCEN/TS 17701-2:2021
---------------------- Page: 2 ----------------------
kSIST-TS FprCEN/TS 17701-2:2021
FINAL DRAFT
TECHNICAL SPECIFICATION
FprCEN/TS 17701-2
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
September 2021
ICS 65.080
English Version
Plant biostimulants - Determination of specific elements -
Part 2: Determination of total content of Cd, Pb, Ni, As, Cr,
Cu and Zn
Biostimulants des plantes - Détermination des Biostimulanzien für die pflanzliche Anwendung -
éléments spécifiques - Partie 2 : Détermination du Bestimmung spezifischer Elemente - Teil 2:
contenu total en Cd, Pb, Ni, As, Cr, Cu et Zn Bestimmung des Gesamtgehaltes an Cd, Pb, Ni, As, Cr,
Cu und Zn
This draft Technical Specification is submitted to CEN members for Vote. It has been drawn up by the Technical Committee
CEN/TC 455.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change
without notice and shall not be referred to as a Technical Specification.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. FprCEN/TS 17701-2:2021 E
worldwide for CEN national Members.
---------------------- Page: 3 ----------------------
kSIST-TS FprCEN/TS 17701-2:2021
FprCEN/TS 17701-2:2021 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Interferences . 6
5.1 General . 6
5.2 Spectral interferences . 6
5.3 Transport interferences . 6
5.4 Excitation interferences . 6
5.5 Chemical interferences . 7
5.6 Memory interferences . 7
6 Reagents . 7
7 Apparatus . 8
7.1 Common laboratory glassware. . 8
7.2 Inductively coupled plasma atomic emission spectrometer. . 8
8 Procedure. 8
8.1 Preparation of test and blank solution . 8
8.2 Preparation of the calibration solutions . 8
8.3 Measurement . 9
8.3.1 Instrument conditions . 9
8.3.2 Optimization of the instrument conditions . 9
8.3.3 Interferences . 9
8.3.4 Measurement . 9
8.3.5 Matrix effects . 9
8.3.6 Spiking. 10
9 Calculation and expression of the results . 11
10 Test report . 12
Bibliography . 13
2
---------------------- Page: 4 ----------------------
kSIST-TS FprCEN/TS 17701-2:2021
FprCEN/TS 17701-2:2021 (E)
European foreword
This document (FprCEN/TS 17701-2:2021) has been prepared by the Technical Committee CEN/TC 455
“Plant Biostimulants”, the secretariat of which is held by AFNOR.
This document is currently submitted to the Vote on TS.
The CEN/TS 17701 series, Plant biostimulants - Determination of specific elements, consists of the
following parts:
— CEN/TS 17701-1, Plant biostimulants - Determination of specific elements - Part 1: Digestion by aqua
regia for subsequent determination of elements;
— CEN/TS 17701-2, Plant biostimulants - Determination of specific elements - Part 2: Determination of
total content of Cd, Pb, Ni, As, Cr, Cu and Zn;
— CEN/TS 17701-3, Plant biostimulants - Determination of specific elements - Part 3: Determination of
mercury.
3
---------------------- Page: 5 ----------------------
kSIST-TS FprCEN/TS 17701-2:2021
FprCEN/TS 17701-2:2021 (E)
Introduction
This document was prepared by the experts of CEN/TC 455 ‘Plant Biostimulants’. The European
Committee for Standardization (CEN) was requested by the European Commission (EC) to draft
European standards or European standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 laying down rules on the making available on the market of
EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This request, presented as SR M/564, also contributes to the Communication on “Innovating for
Sustainable Growth: A Bio economy for Europe”. The Working Group 4 “Other safety parameters” was
created to develop a work program as part of this Request. The technical committee CEN/TC 455 ‘Plant
Biostimulants’ was established to carry out the work program that will prepare a series of standards. The
interest in biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
The preparation of this document is based on a standardization request to CEN by the European
Commission and the European Free Trade Association (Mandate M/564) concerning the modernization
of methods of analysis of fertilizers in the framework of Regulation (EU) 2019/1009 of the European
Parliament and of the Council.
This document is part of a modular approach and concerns the analytical measurement step. “Modular”
means that a test standard concerns a specific step in assessing a property and not the whole chain of
measurements. Inductively coupled plasma atomic emission spectrometry (ICP-AES) is nowadays widely
used and a well-established method in many laboratories.
WARNING – Persons using this document should be familiar with usual laboratory practice. This
document does not purport to address all of the safety issues, if any, associated with its use. It is
the responsibility of the user to establish appropriate health and safety practices and to ensure
compliance with any national regulatory conditions.
IMPORTANT – It is absolutely essential that tests conducted according to this document are
carried out by suitably trained staff.
4
---------------------- Page: 6 ----------------------
kSIST-TS FprCEN/TS 17701-2:2021
FprCEN/TS 17701-2:2021 (E)
1 Scope
This document specifies a method for the determination of arsenic (As), cadmium (Cd), copper (Cu),
chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in plant biostimulant digests using inductively coupled
plasma-atomic emission spectrometry (ICP-AES).
This document is also applicable to the blends of fertilizing products where plant biostimulants are the
main part of the blend. Otherwise, the Technical Specification for the main part of the blend applies.
This method is applicable to aqua regia digests prepared according to CEN/TS 17701-1. The method can
be used for the determination of other elements, provided the user has verified the applicability.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
CEN/TS 17701-1, Plant biostimulants - Determination of specific elements - Part 1: Digestion by aqua regia
for subsequent determination of elements
CEN/TS 17704, Plant biostimulants - Determination of dry matter
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
analyte
parameter to be determined
3.2
blank calibration solution
solution prepared in the same way as the calibration solution but leaving out the analytes
3.3
blank test solution
solution prepared in the same way as the test sample solution but omitting the test portion
3.4
calibration solution
solution used to calibrate the instrument, prepared from stock solutions by adding acids, buffer, reference
element and salts as needed
3.5
digest
solution received after mineralization of the organic matter of a sample and dissolution of its mineral
part, more or less completely, when reacting with a reagent mixture
3.6
stock solution
solution with accurately known analyte concentration(s), prepared from pure chemicals (6.5)
3.7
test sample solution
solution prepared after extraction or digestion of the test sample according to appropriate specifications
5
---------------------- Page: 7 ----------------------
kSIST-TS FprCEN/TS 17701-2:2021
FprCEN/TS 17701-2:2021 (E)
4 Principle
The method is based on the inductively coupled plasma atomic emission spectrometry (ICP-AES)
measurement of the concentration of arsenic, cadmium, copper, chromium, lead, nickel and zinc in plant
biostimulant aqua regia digests. The elements are determined after an appropriate dilution of the digest,
if necessary. The solution is dispersed by a suitable nebulizer of the ICP-AES instrument and the resulting
aerosol is transported into the plasma torch. Element specific emission spectra are produced by a
radiofrequency inductively coupled argon plasma where atoms or ions are excited at high temperature.
The emission spectra are dispersed by a spectrometer, and the intensities of the emission lines are
monitored by photosensitive devices. Multi-element determinations using sequential or simultaneous
optical systems and axial, radial or dual viewing of the plasma may be used.
5 Interferences
5.1 General
Interferences and matrix effects shall be recognized and appropriate measures to minimize them shall be
made. There are several types of interferences – see 5.2 – 5.6.
5.2 Spectral interferences
Spectral interferences are due to incomplete isolation of the radiation emitted by the analyte from other
detected radiation sources. Spectral interferences are caused by the background emission from
continuous or recombination phenomena, by stray light which causes background increase or overlap of
a spectral line from another element, or by the unresolved overlap of molecular band spectra. Background
emission and stray light can usually be compensated for by subtracting the background emission
measured adjacent to the analyte wavelength peak. To correct a sloping background shift background
correction points on each side of the peak are used. The increase of background is more intensive with
axial-view instruments. Background correction is not required in cases of line broadening where a
background correction measurement would actually degrade the analytical result. A spectral line overlap
usually leads to the choice of an alternative line. If this is not possible, mathematical correction
procedures (e.g. inter-element correction technique or multi-component spectral fitting) can be used to
compensate for the interference. These correction procedures are usually a part of the instrument
software.
5.3 Transport interferences
Transport interferences are caused by differences in the properties between the sample solutions and
the calibration solutions (viscosity, surface tension, density, dissolved solid content, type and
concentration of acids). As a consequence, the supply of solutio
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.