SIST EN ISO 6529:2002
(Main)Protective clothing - Protection against chemicals - Determination of resistance of protective clothing materials to permeation by liquids and gases (ISO 6529:2001)
Protective clothing - Protection against chemicals - Determination of resistance of protective clothing materials to permeation by liquids and gases (ISO 6529:2001)
This standard describes laboratory test methods that enable a determination of the resistance of materials used in protective clothing to permeation by liquid or gaseous chemical under the conditions of either continuous or intermittent contact.
Schutzkleidung - Schutz gegen Chemikalien - Bestimmung des Widerstands von Schutzkleidungsmaterialien gegen die Permeation von Flüssigkeiten und Gasen (ISO 6529:2001)
Diese Internationale Norm beschreibt Laborprüfverfahren, die eine Bestimmung des Widerstands von Materialien gestattet, die für Schutzkleidung gegen die Permeation von flüssigen oder gasförmigen Chemikalien unter Bedingungen mit kontinuierlichem oder diskontinuierlichem Kontakt verwendet werden.
Verfahren A (8.3) ist anwendbar bei der Prüfung von flüssigen Chemikalien, die entweder flüchtig oder in Wasser löslich sind, wenn ein kontinuierlicher Kontakt des chemischen Stoffes mit dem Schutzkleidungsmaterial besteht.
Verfahren B (8.4) ist anwendbar bei der Prüfung von gasförmigen Chemikalien, wenn ein kontinuierlicher Kontakt des chemischen Stoffes mit dem Schutzkleidungsmaterial besteht.
Verfahren C (8.5) ist anwendbar bei der Prüfung von flüssigen Chemikalien, die entweder flüchtig oder in Wasser oder Gas löslich sind, wenn ein diskontinuierlicher Kontakt des chemischen Stoffes mit dem Schutzkleidungs-material besteht.
Diese Prüfverfahren sind nur für die Prüfung luftundurchlässiger Schutzkleidungsmaterialien geeignet. Sie beurteilen den Permeationswiderstand von dem Schutzkleidungsmaterial unter Laborbedingungen in Bezug auf Durchbruchszeit, Permeationsgeschwindigkeit und kumulative Permeation. Diese Prüfverfahren ermöglichen auch das Beobachten der Auswirkungen der Prüfflüssigkeit auf das zu prüfende Schutzkleidungsmaterial.
Diese Prüfverfahren gelten nur für die Gebrauchstauglichkeit von Materialien oder bestimmten Materialaufbauten (z. B. Nähte), die für Schutzkleidung verwendet werden. Diese Prüfverfahren beziehen sich nicht auf Gestaltung, Gesamtaufbau und Bestandteile oder Grenzflächen von Kleidungsstücken oder weitere Faktoren, die den durch die Schutzkleidung gebotenen Gesamtschutz beeinflussen.
Vetements de protection - Protection contre les produits chimiques - Détermination de la résistance des matériaux utilisés pour la confection des vetements de protection a la perméation par des liquides et des gaz (ISO 6529:2001)
La présente Norme internationale décrit des méthodes d'essai en laboratoire permettant de déterminer, pour les matériaux utilisés dans les vêtements de protection, la résistance à la perméation par les produits chimiques liquides ou gazeux dans des conditions de contact continu ou intermittent. La méthode A est applicable quand le produit chimique d'essai est un liquide, volatil ou soluble dans l'eau, destiné à être en contact continu avec le matériau du vêtement de protection.La méthode B est applicable quand le produit chimique d'essai est un gaz, destiné à être en contact continu avec le matériau du vêtement de protection.La méthode C est applicable quand le produit chimique d'essai est un liquide, volatil ou soluble dans l'eau, destiné à être en contact intermittent avec le matériau du vêtement de protection.Ces méthodes d'essai ne se prêtent qu'aux essais de matériaux de protection imperméables à l'air. Elles permettent de déterminer la résistance à la perméation du matériau du vêtement de protection dans des conditions de laboratoire en termes de temps de passage, de flux de perméation et de perméation cumulée. Ces méthodes d'essai permettent également d'observer les effets du liquide d'essai sur le matériau du vêtement de protection soumis à essai.Ces méthodes d'essai ne traitent que de la performance des matériaux ou de certains types de fabrication de matériaux (coutures par exemple) utilisés dans les vêtements de protection. Ces méthodes d'essai ne couvrent pas la conception, la fabrication générale ou les composants, ni les zones de jonction de vêtements ou autres facteurs à même d'avoir un effet sur la protection globale offerte par le vêtement de protection.Il est à signaler que ces essais ne simulent pas nécessairement les conditions dans lesquelles les matériaux du vêtement de protection sont susceptibles d'être exposés dans la pratique.Il convient par conséquent de limiter l'utilisation des données d'essai à une simple évaluation comparative de ce
Varovalna obleka - Varovanje pred kemikalijami - Ugotavljanje odpornosti materialov za varovalne obleke proti prepustnosti tekočin in plinov (ISO 6529:2001)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 6529:2002
01-junij-2002
1DGRPHãþD
SIST EN 369:1996
9DURYDOQDREOHND9DURYDQMHSUHGNHPLNDOLMDPL8JRWDYOMDQMHRGSRUQRVWL
PDWHULDORY]DYDURYDOQHREOHNHSURWLSUHSXVWQRVWLWHNRþLQLQSOLQRY ,62
Protective clothing - Protection against chemicals - Determination of resistance of
protective clothing materials to permeation by liquids and gases (ISO 6529:2001)
Schutzkleidung - Schutz gegen Chemikalien - Bestimmung des Widerstands von
Schutzkleidungsmaterialien gegen die Permeation von Flüssigkeiten und Gasen (ISO
6529:2001)
Vetements de protection - Protection contre les produits chimiques - Détermination de la
résistance des matériaux utilisés pour la confection des vetements de protection a la
perméation par des liquides et des gaz (ISO 6529:2001)
Ta slovenski standard je istoveten z: EN ISO 6529:2001
ICS:
13.340.10 Varovalna obleka Protective clothing
SIST EN ISO 6529:2002 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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EUROPEAN STANDARD
EN ISO 6529
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2001
ICS 13.340.10 Supersedes EN 369:1993
English version
Protective clothing - Protection against chemicals -
Determination of resistance of protective clothing materials to
permeation by liquids and gases (ISO 6529:2001)
Vêtements de protection - Protection contre les produits Schutzkleidung - Schutz gegen Chemikalien - Bestimmung
chimiques - Détermination de la résistance des matériaux des Widerstands von Schutzkleidungsmaterialien gegen
utilisés pour la confection des vêtements de protection à la die Permeation von Flüssigkeiten und Gasen (ISO
perméation par des liquides et des gaz (ISO 6529:2001) 6529:2001)
This European Standard was approved by CEN on 4 October 2001.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2001 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6529:2001 E
worldwide for CEN national Members.
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EN ISO 6529:2001 (E)
CORRECTED 2002-10-16
Foreword
This document (ISO 6529:2001) has been prepared by Technical Committee ISO/TC 94
"Personal safety - Protective clothing and equipment" in collaboration with Technical
Committee CEN/TC 162 "Protective clothing including hand and arm protection and
lifejackets", the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by April 2002, and conflicting national
standards shall be withdrawn at the latest by April 2002.
This document supersedes EN 369:1993.
This document has been prepared under a mandate given to CEN by the European
Commission and the European Free Trade Association, and supports essential requirements
of EU Directive(s).
For relationship with EU Directive(s), see informative annex ZB, which is an integral part of
this document.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United
Kingdom.
Endorsement notice
The text of ISO 6529:2001 has been approved by CEN as EN ISO 6529:2001 without any
modifications.
NOTE Normative references to International Standards are listed in Annex ZA (normative).
2
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EN ISO 6529:2001 (E)
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions
of any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications,
indicated by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 139 1973 Textiles - Standard atmospheres EN 20139 1992
for conditioning and testing
ISO 2286-2 1998 Rubber- or plastics-coated fabrics EN ISO 2286-2 1998
- Determination of roll
characteristics - Part 2: Methods
for determination of total mass per
unit area, mass per unit area of
coating and mass per unit area of
substrate
ISO 2286-3 1998 Rubber- or plastics-coated fabrics EN ISO 2286-3 1998
- Determination of roll
characteristics - Part 3: Method for
determination of thickness
ISO 5084 1996 Textiles - Determination of EN ISO 5084 1996
thickness of textiles and textile
products
3
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EN ISO 6529:2001 (E)
Annex ZB
(informative)
Clauses of this European Standard addressing essential requirements or other
provisions of EU Directives
By agreement between ISO and CEN, this CEN annex is included in the DIS and the FDIS
but will not appear in the published ISO standard.
This European Standard has been prepared under a mandate given to CEN by the European
Commission and supports essential requirements of EU Directive 89/686/EEC, Annex II.
Note Other requirements and other EU Directives may be applicable to the product(s)
falling within the scope of this standard.
Subclause 3.10.2 of this standard is likely to support requirements of EU Directive
89/686/EEC, Annex II.
Compliance with this subclause provides one means of conforming with the specific essential
requirements of the Directive concerned.
4
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INTERNATIONAL ISO
STANDARD 6529
Second edition
2001-10-15
Protective clothing — Protection against
chemicals — Determination of resistance of
protective clothing materials to permeation
by liquids and gases
Vêtements de protection — Protection contre les produits chimiques —
Détermination de la résistance des matériaux utilisés pour la confection
des vêtements de protection à la perméation par des liquides et des gaz
Reference number
ISO 6529:2001(E)
©
ISO 2001
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ISO 6529:2001(E)
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ii © ISO 2001 – All rights reserved
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ISO 6529:2001(E)
Contents Page
Foreword.iv
Introduction.v
1 Scope .1
2 Normative references .1
3 Terms and definitions .2
4 Principle.5
5 Choice of analytical technique and collection medium.5
5.1 General.5
5.2 Gaseous collection medium.5
5.3 Liquid collection medium .6
5.4 Other collection medium.6
6 Apparatus .6
7 Sampling.11
7.1 Sampling procedure .11
7.2 Preparation of test specimens .12
7.3 Measurement of test specimen thickness and mass.12
8 Procedure .12
8.1 Calibration .12
8.2 Preparation of test apparatus.12
8.3 Method A — Liquid chemicals with continuous contact.14
8.4 Method B — Gaseous chemicals with continuous contact .14
8.5 Method C — Liquid or gaseous chemical with intermittent contact .16
8.6 Preparation of permeation plot .17
8.7 Determination of breakthrough detection time and normalized breakthrough detection time.17
8.8 Calculation of permeation rate and cumulative permeation .18
8.9 Visual assessment of test specimen .19
8.10 Repeat tests.19
9 Report .20
Annex A (informative) Recommended list of chemicals for comparing permeation resistance of
protective clothing materials.22
Annex B (informative) Precision information for test method.24
Annex C (informative) Sources of permeation test cells and permeation test cell parts .25
Annex D (informative) Suggested procedure for measuring the sensitivity of open-loop permeation-
test systems .26
Bibliography.29
© ISO 2001 – All rights reserved iii
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ISO 6529:2001(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 6529 was prepared by Technical Committee ISO/TC 94, Personal safety — Protective
clothing and equipment, Subcommittee SC 13, Protective clothing.
This second edition cancels and replaces the first edition (ISO 6529:1990), which has been technically revised.
Annexes A to D of this International Standard are for information only.
iv © ISO 2001 – All rights reserved
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ISO 6529:2001(E)
Introduction
Workers involved in the production, use, transportation, and emergency response with liquid and gaseous
chemicals can be exposed to numerous compounds capable of causing harm upon contact with the human body.
The deleterious effects of these chemicals can range from acute trauma such as skin irritation and burn to chronic
degenerative disease, such as cancer. Since engineering controls may not eliminate all possible exposures,
attention is often placed on reducing the potential for direct skin contact through the use of protective clothing that
resists permeation, penetration and degradation.
These test methods are normally used to evaluate the barrier effectiveness of materials used for protective clothing
and specimens from finished items (see Note 1) of protective clothing against permeation of either liquid or
gaseous chemicals. Options are provided for conducting this testing under both conditions of continuous or
intermittent contact with the chemicals.
These test methods provide various options for reporting test results in terms of breakthrough time, permeation rate
and cumulative permeation to allow a comparison of protective clothing material permeation resistance. These
parameters are key measures of the effectiveness of a clothing material to act as a barrier to the test chemical.
Such information is used in the comparison of clothing materials during the process of selecting clothing for
protection from hazardous chemicals. Long breakthrough detection times and normalized breakthrough detection
times as well as low permeation rates are characteristic of the best barriers.
Resistance to penetration by liquid chemicals should be determined by using ISO 6530 while resistance to
penetration by liquid chemicals under pressure should be determined by using ISO 13994. These International
Standards are listed in the Bibliography.
It has been assumed in the drafting of this International Standard that the execution of its provisions is entrusted to
appropriately qualified and experienced people for whose guidance it has been prepared and that appropriate
precautions will be taken to avoid injury to health and contamination of the environment.
NOTE 1 Finished items of protective clothing include gloves, arm shields, aprons, suits, hoods, boots, etc. The phrase
“specimens from finished items” encompasses seamed and other discontinuous regions as well as the usual continuous regions
of protective clothing items.
NOTE 2 At present, no quantitative information exists about acceptable levels of dermal contact. Therefore, the data obtained
using this test method cannot be used to infer safe exposure levels.
© ISO 2001 – All rights reserved v
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INTERNATIONAL STANDARD ISO 6529:2001(E)
Protective clothing — Protection against chemicals —
Determination of resistance of protective clothing materials to
permeation by liquids and gases
1 Scope
This International Standard describes laboratory test methods that enable a determination of the resistance of
materials used in protective clothing to permeation by liquid or gaseous chemicals under the conditions of either
continuous or intermittent contact.
Method A (see 8.3) is applicable to the testing of liquid chemicals, either volatile or soluble in water, expected to be
in continuous contact with the protective clothing material.
Method B (see 8.4) is applicable to the testing of gaseous chemicals expected to be in continuous contact with the
protective clothing material.
Method C (see 8.5) is applicable to the testing of liquid chemicals, either volatile or soluble in water, expected to be
in intermittent contact with the protective clothing material.
These test methods are only suitable for the testing of air-impermeable protective clothing materials. They assess
the permeation resistance of the protective clothing material under laboratory conditions in terms of breakthrough
time, permeation rate, and cumulative permeation. These test methods also enable observations to be made of the
effects of the test liquid on the protective clothing material under test.
These test methods address only the performance of materials or certain material constructions (e.g. seams) used
in protective clothing. These test methods do not address the design, overall construction and components, or
interfaces of garments or other factors which may affect the overall protection offered by the protective clothing.
It is emphasized that these tests do not necessarily simulate conditions to which clothing materials are likely to be
exposed in practice. The use of test data should therefore be restricted to broad comparative assessment of such
material according to their permeation-resistance characteristics.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent editions of the normative documents indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 139, Textiles — Standard atmospheres for conditioning and testing
ISO 2286-2, Rubber- or plastics-coated fabrics — Determination of roll characteristics — Part 2: Methods for
determination of total mass per unit area, mass per unit area of coating and mass per unit area of substrate
ISO 2286-3, Rubber- or plastics-coated fabrics — Determination of roll characteristics — Part 3: Method for
determination of thickness
© ISO 2001 – All rights reserved 1
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ISO 6529:2001(E)
ISO 3801, Textiles — Woven fabrics — Determination of mass per unit length and mass per unit area
ISO 5084, Textiles — Determination of thickness of textiles and textile products
3 Terms and definitions
For the purposes of this International Standard, the following terms and definitions apply.
3.1
analytical technique
procedure whereby the concentration of a chemical in a collection medium is quantitatively determined
NOTE These procedures are often specific to individual chemical and collection-medium combinations.
EXAMPLES Applicable analytical techniques can include ultraviolet (UV) and infrared (IR) spectrophotometry, gas and
liquid chromatography, pH measurement, ion chromatography, conductimetry, colorimetry, atmospheric analytical detector
tubes and radionuclide tagging/detection counting.
3.2
breakthrough detection time
elapsed time measured from the start of the test to the sampling time that immediately precedes the sampling time
at which the test chemical is first detected
SeeFigure1.
NOTE The breakthrough detection time is dependent on the sensitivity of the method and the frequency of sampling (the
interval between sampling times).
3.3
closed-loop
refers to a testing mode in which the collection medium volume is fixed
NOTE The collection medium volume may change slightly from sampling without replacement of the sampled collection
medium.
3.4
collection medium
liquid or gas that does not affect the measured permeation and in which the test chemical is freely soluble or
adsorbed to a saturation concentration greater than 0,5 % by mass or by volume
3.5
contact time
in an intermittent contact test, the duration that the challenge-side chamber of the permeation cell contains test
chemical during each cycle
3.6
cumulative permeation mass
total amount of chemical that permeates during a specified time from the time the clothing material specimen is first
contacted with the test chemical
NOTE 1 Quantification of cumulative permeation enables the comparison of permeation behaviour under different intermittent
and continuous-contact conditions.
NOTE 2 The measurement of cumulative permeation may depend on the sensitivity of the permeation-test system.
3.7
cycle time
in an intermittent contact permeation test, the interval of time from the start of one contact period to the start of the
next contact period
2 © ISO 2001 – All rights reserved
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ISO 6529:2001(E)
2
NOTE The breakthrough detection time for a method sensitivity of 0,05 �g/cm /min is 23 min but would be reported at
20 min, which corresponds to the last sampling time preceding the test. The normalized breakthrough detection time at a
2
normalization permeation rate of 0,1 �g/cm /min is 33 min, but similarly would be reported at 28 min, which corresponds to the
2
preceding sampling time. The steady-state permeation rate is approximately 0,15 �g/cm /min.
Figure 1 — Breakthrough detection time
3.8
degradation
deleterious change in one or more physical properties of a protective clothing material
3.9
minimum detectable mass permeated
smallest mass of test chemical that is detectable with the complete permeation-test system
NOTE This value is not necessarily the intrinsic limit of detection for the analytical instrument.
3.10
minimum detectable permeation rate
lowest rate of permeation that is measurable with the complete permeation-test system
NOTE This value is not necessarily the intrinsic limit of detection for the analytical instrument.
3.11
normalization permeation mass
permeation mass used for determining the normalized breakthrough detection time in a closed-loop permeation test
© ISO 2001 – All rights reserved 3
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ISO 6529:2001(E)
2 2
NOTE This test method provides two choices of normalization permeation mass at 0,25 �g/cm or 2,5 �g/cm .
3.12
normalization permeation rate
permeation rate used for determining the normalized breakthrough detection time in an open-loop permeation test
2 2
NOTE This test method provides two choices of normalization permeation rates: 0,1 �g/cm /min or 1,0 �g/cm /min.
3.13
normalized breakthrough detection time
�open-loop system� time at which the permeation rate reaches the normalization permeation rate
SeeFigure1.
3.14
normalized breakthrough detection time
�closed-loop test� time at which the mass of chemical permeated reaches the normalization permeation mass
3.15
open-loop
testing mode in which fresh collection medium flows continuously through the collection chamber of the test cell
and is not reused or recycled
3.16
penetration
flow of a chemical through closures, porous materials, seams and holes or other imperfections in a protective
clothing material on a non-molecular level
3.17
permeation
process by which a chemical moves through a protective clothing material on a molecular level
NOTE Permeation involves
a) sorption of molecules of the chemical into the contacted (outside) surface of a material,
b) diffusion of the sorbed molecules in the material, and
c) desorption of the molecules from the opposite (inside) surface of the material into the collection medium.
3.18
permeation mass
quantity of test chemical that passes through the protective clothing material within a given time
3.19
permeation rate
quantity of test chemical that passes through the protective clothing material for a given exposed surface area per
unit time
3.20
protective clothing material
any material or combination of materials used in an item of clothing for the purpose of isolating parts of the body
from a potential hazard
3.21
purge time
�intermittent contact test� time immediately following the termination of the contact time when the test chemical is
removed from the challenge-side chamber and air or nitrogen is blown over the outside surface of the protective
clothing material
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ISO 6529:2001(E)
3.22
steady-state permeation rate
constant rate of permeation that occurs after breakthrough when the chemical contact is continuous and all forces
affecting permeation have reached equilibrium
NOTE Steady-state permeation may not be achieved during the period for which permeation testing is conducted.
3.23
test chemical
liquid or gas that is used to challenge the protective clothing material specimen
NOTE The liquid or gas may be either one component (i.e. a neat liquid or gas) or have several components (i.e. a mixture).
4Principle
The protective clothing material specimen acts as a partition between one chamber of a permeation test cell, which
contains the test chemical, and another chamber, which contains the collection medium.
The test chemical may be either a liquid or a gas. The protective clothing material specimen may contact the test
chemical either continuously or intermittently depending on the choice of the method used.
The collection medium, which may be liquid or gas, is analysed quantitatively for its concentration of the chemical
and thereby the amount of that chemical that has permeated the partition as a function of time after its initial
contact with the material.
Several different test configurations may be used depending on the choice of the test chemical, collection medium
and conditions of the test.
By either graphical representation or appropriate calculations, or both, the breakthrough detection time, normalized
breakthrough detection time, permeation rate, and cumulative permeation of the test chemical are determined.
A group of chemicals, representing a range in chemical properties, which can be used to compare the permeation
resistance is given in annex A.
Interlaboratory data for this test method are provided in annex B.
5 Choice of analytical technique and collection medium
5.1 General
The combination of the analytical technique and the collection medium shall be selected to maximize sensitivity for
the detection of the test chemical
...
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