SIST EN ISO 21265:2024

Building and civil engineering sealants - Assessment of the fungal growth on sealant surfaces (ISO 21265:2021)

This document specifies a method for the evaluation of the fungal growth on sealants which are used in joints in building construction.

Dichtstoffe im Hoch- und Tiefbau - Prüfmethode zur Bestimmung des Pilzwachstums auf Dichtstoffoberflächen (ISO 21265:2021)

Dieses Dokument legt ein Verfahren zur Bewertung von Pilzwachstum auf Dichtstoffen fest, die zur Abdichtung von Fugen im Hochbau verwendet werden.

Mastics pour le bâtiment et le génie civil - Évaluation de la croissance fongique à la surface des mastics (ISO 21265:2021)

Tesnilne mase za stavbe in gradbene inženirske objekte - Ocena rasti glivic na površinah tesnilnih mas (ISO 21265:2021)

Ta dokument določa metodo za oceno rasti glivic v tesnilih, ki se uporabljajo za stike v stavbah.

General Information

• Status: Published
• Public Enquiry End Date: 02-Apr-2024
• Publication Date: 29-May-2024
• ICS: 91.100.50 - Binders. Sealing materials
• Technical Committee: VLA - Water proofing
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 16-May-2024
• Due Date: 21-Jul-2024
• Completion Date: 30-May-2024
• Ref Project: EN ISO 21265:2024 - Building and civil engineering sealants - Assessment of the fungal growth on sealant surfaces (ISO 21265:2021)

Overview

EN ISO 21265:2024 (identical to ISO 21265:2021) defines a laboratory test method for assessing fungal growth on building and civil engineering sealant surfaces. Adopted by CEN in May 2024, this standard specifies procedures to evaluate whether sealants used in joints and façades resist colonization by common fungal species under controlled humidity and temperature. The standard supports product performance assessment, quality control and durability evaluation for sealants exposed to high-humidity service conditions.

Key topics and technical requirements

• Scope: Method for evaluating fungal growth on sealant surfaces used in building construction joints.
• Test organisms: The procedure targets the five commonly encountered fungal strains (examples given in the standard): Fusarium oxysporum, Aspergillus niger, Phoma herbarum, Exophiala jeanselmei, Penicillium chrysogenum (specified by culture collection accession numbers such as ATCC strains).
• Apparatus & conditions: Requirements include incubators (20–35 °C, ±1 °C) with ≥90% relative humidity, ovens (45 ±2 °C), autoclave sterilization (120 ±2 °C, 2 bar), centrifuge, stereomicroscope (×50), analytical balance and sterile Petri dishes.
• Specimen preparation: Conditioning, cleaning, labelling and optional water-immersion steps are specified to simulate service conditions.
• Test procedures: Inoculation methods, spore suspension preparation, viability checks, incubation and arrangement of specimens are detailed to ensure reproducible fungal exposure.
• Assessment & reporting: Visual assessment of fungal growth, measurement of inhibition zones (if any), recording of results and required test-report content are specified for consistent interpretation.

Applications and practical value

• Product development and R&D: Screen formulations for fungistatic/fungicidal properties during formulation and accelerated ageing.
• Quality control and type testing: Verify batch-to-batch consistency and confirm performance claims regarding microbial resistance.
• Specification and procurement: Provide objective test evidence for material selection in high-humidity environments (bathrooms, kitchens, façades, tunnels).
• Compliance and risk mitigation: Support performance-based specifications and help reduce biological staining, odour and material degradation in buildings.

Who should use this standard

• Sealant manufacturers and R&D labs
• Independent test laboratories and certification bodies
• Architects, specifiers and façade engineers
• Building owners, facility managers and contractors concerned with durability in humid conditions
• National standards bodies and regulatory authorities

Related standards

• ISO 846 - Plastics: evaluation of the action of microorganisms (referenced)
• ISO 6927 - Building and civil engineering sealants: vocabulary (referenced)
• EN adoption noted as EN ISO 21265:2024

Using EN ISO 21265:2024 helps stakeholders produce reproducible, comparable fungal resistance data for sealants - essential for durable, hygienic and low-maintenance building joints.

Standards Content (Sample)

SLOVENSKI STANDARD
01-julij-2024
Tesnilne mase za stavbe in gradbene inženirske objekte - Ocena rasti glivic na
površinah tesnilnih mas (ISO 21265:2021)
Building and civil engineering sealants - Assessment of the fungal growth on sealant
surfaces (ISO 21265:2021)
Dichtstoffe im Hoch- und Tiefbau - Prüfmethode zur Bestimmung des Pilzwachstums auf
Dichtstoffoberflächen (ISO 21265:2021)
Mastics pour le bâtiment et le génie civil - Évaluation de la croissance fongique à la
surface des mastics (ISO 21265:2021)
Ta slovenski standard je istoveten z: EN ISO 21265:2024
ICS:
91.100.50 Veziva. Tesnilni materiali Binders. Sealing materials
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 21265
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2024
EUROPÄISCHE NORM
ICS 91.100.50
English Version
Building and civil engineering sealants - Assessment of the
fungal growth on sealant surfaces (ISO 21265:2021)
Mastics pour le bâtiment et le génie civil - Évaluation Dichtstoffe im Hoch- und Tiefbau - Prüfmethode zur
de la croissance fongique à la surface des mastics (ISO Bestimmung des Pilzwachstums auf
21265:2021) Dichtstoffoberflächen (ISO 21265:2021)
This European Standard was approved by CEN on 5 May 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21265:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 21265:2021 has been prepared by Technical Committee ISO/TC 59 "Sealants” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 21265:2024 by
Technical Committee CEN/TC 349 “Sealants for joints in building construction” the secretariat of which
is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2024, and conflicting national standards
shall be withdrawn at the latest by November 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 21265:2021 has been approved by CEN as EN ISO 21265:2024 without any modification.

INTERNATIONAL ISO
STANDARD 21265
First edition
2021-09
Building and civil engineering
sealants — Assessment of the fungal
growth on sealant surfaces
Mastics pour le bâtiment et le génie civil — Évaluation de la
croissance fongique à la surface des mastics
Reference number
ISO 21265:2021(E)
©
ISO 2021
ISO 21265:2021(E)
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2021 – All rights reserved

ISO 21265:2021(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Apparatus . 1
5 Test fungi preparation . 2
5.1 Test fungi . 2
5.2 Stock strains . 2
5.3 Solutions and nutritive media . 3
6 Preparation of test specimens . 3
6.1 General . 3
6.2 Preparation and conditioning . 3
6.3 Cleaning, labelling and storage of specimens . 4
6.3.1 Cleaning . 4
6.3.2 Labelling and storage . 4
7 Test procedures . 4
7.1 General . 4
7.2 Test set-up and inoculation of test specimens . 5
7.2.1 Specimens with or without water immersion . 5
7.2.2 Test with solid medium: filling the petri dishes . 6
7.2.3 Test for without solid-medium (optional) . 6
7.2.4 Arrangement of test specimens . 6
7.2.5 Preparation of spore suspension . 6
7.2.6 Harvesting the spores . 6
7.2.7 Washing the spores by centrifugation and preparation of working suspensions . 6
7.2.8 Spore viability check . 7
7.2.9 Inoculation of specimens . 7
7.2.10 Incubation . 7
8 Assessments . 7
8.1 Assessment of fungal growth on the specimens by visual examination . 7
8.2 Assessment of inhibition zone . 8
8.3 Record the test result . 9
9 Expression of results . 9
10 Test report . 9
ISO 21265:2021(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 59, Buildings and civil engineering works,
Subcommittee SC 8, Sealants.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2021 – All rights reserved

ISO 21265:2021(E)
Introduction
Sealants used in high humidity environments experience growth of microorganisms; and it can be
necessary to test the function of sealant under normal service conditions to ensure that the sealant
surface remains free of the growth microorganisms over a designated function period. This test is
designed to evaluate the 5 most common strains of microorganisms found on sealant surfaces.
INTERNATIONAL STANDARD ISO 21265:2021(E)
Building and civil engineering sealants — Assessment of
the fungal growth on sealant surfaces
1 Scope
This document specifies a method for the evaluation of the fungal growth on sealants which are used in
joints in building construction.
2 Normative references
The following documents are referred to in the text in such a way that some or all of th
...