SIST ISO 12771:1998
(Main)Plastics laboratory ware -- Disposable serological pipettes
Plastics laboratory ware -- Disposable serological pipettes
Matériel de laboratoire en plastique -- Pipettes sérologiques à usage unique
Plastična laboratorijska oprema - Serološke pipete za enkratno uporabo
General Information
Standards Content (Sample)
INTERNATIONAL
IS0
STANDARD
12771
First edition
1997-05-01
Plastics laboratory ware - Disposable
serological pipettes
Matkriel de laboratoire en plastique - Pipettes s6rologiques h usage
unique
Reference number
IS0 12771:1997(E)
---------------------- Page: 1 ----------------------
IS0 12771 :1997(E)
Foreword
IS0 (the International Organization for Standardization) is a worldwide federation of national standards bodies
(IS0 member bodies). The work of preparing International Standards is normally carried out through IS0
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. IS0 collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are circulated to the member bodies for
voting. Publication as an International Standard requires approval by at least 75 % of the member bodies
casting a vote.
International Standard IS0 12771 was prepared by Technical Committee lSO/TC 48, Laboratory glassware and
related apparatus, Subcommittee SC 1, Volumetric instruments.
Annex A of this International Standard is for information only.
0 IS0 1997
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronic or mechanical, including photocopying and
microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case postale 56 l CH-1211 Geneve 20 l Switzerland
Internet central @ isoch
x.400 c=ch; a=400net; p=iso; o=isocs; s=central
Printed in Switzerland
ii
---------------------- Page: 2 ----------------------
INTERNATIONAL STANDARD @ IS0 IS0 12771 :1997(E)
Plastics laboratory ware - Disposable serological pipettes
1
Scope
This International Standard specifies requirements for disposable plastics serological pipettes, gauged to
deliver, suitable for general laboratory purposes.
2 Normative references
The following standards contain provisions which, through references in this text, constitute provisions of this
International Standard. At the time of publication, the editions indicated were valid. All standards are subject to
revision, and parties to agreements based on this International Standard are encouraged to investigate the
possibility of applying the most recent editions of the standards indicated below. Members of IEC and IS0
maintain registers of currently valid International Standards.
IS0 1043-I : 1987, Plastics - Symbols - Part 1: Basic polymers and their special characteristics.
IS0 1769: 1975, Laboratory glassware - Pipettes - Colour coding.
IS0 8417:~,” Laboratory volumetric instruments - Principles of design and construction of disposable
volumetric articles .
Definitions
3
For the purposes of this International Standard, the following definitions apply.
3.1 disposable: Adjective used to describe serological pipettes which are intended to be used once only and
then discarded.
NOTE - Such pipettes will only be expected to provide their specified performance during the original operation.
3.2 accuracy (of a pipette): Closeness of agreement between the nominal volume and the true volume,
obtained by applying the test procedure specified in clause 9.1. It is quantified by the capacity deviation.
3.3 repeatability (of a number of pipettes): Closeness of agreement between the individual volumes obtained
by applying the test procedure specified in 9.2.
NOTE 1: Definitions and terms are in agreement with the “International Vocabulary of Basic and General Terms in
Metrology”. See IS0 3534-l for details.
NOTE 2: The definitions for accuracy and repeatability apply only in cases where the distributions are Gaussian.
4 Basis of adjustment
4.1 Unit of volume
The unit of volume is the cubic centimetre (cm”), for which the name millilitre (ml) may be used.
‘) To be published.
---------------------- Page: 3 ----------------------
IS0 12771 :1997(E) 0 IS0
NOTE - The term millilitre (ml) is commonly used as a special name for the cubic centimetre (cm”), in accordance with the
International System of Units (Sl).
4.2 Reference temperature
The reference temperature, i.e. the temperature at which the pipette is intended to deliver its nominal volume
(nominal capacity), is 20 OC.
When the pipette is required for use in a country which has adopted a reference temperature of 27 OC (the
alternative specified in IS0 8417 for tropical use), this figure shall be substituted for 20 OC.
4.3 Adjustment
The pipettes shall be adjusted to deliver the required volume (i.e. the nominal volume or a part of it) with the
last drop to be blown out.
5 Dimensions and delivery times
The dimensions and delivery times shall be as shown in table 1.
The delivery time shall be determined with the pipette unplugged, and with distilled water at a temperature of
2OOC.
Table 1 - Capacity, subdivision, dimensions and delivery times
I I I I
Nominal Smallest Lowest External Wall Delivery
scale division graduation thickness
capacity
times
diameter
line at min.
min. max.
ml mm
ml ml
mm
S
015 0,Ol Of4 4,5 to
4,8 018
1 0,Ol 03 -
4,5to 5,0 03
1
1
OrI 03 4,5to 5,0 018 Of5 1 3,o
2 0,Ol
'13 5,4 to 6,7 03 or5 40
2 w ‘13 5,4 to 6,7 02 Of5 / 0
0,5 1 7,0
5 01 I 0 7,6 to IO,0 03
9,5to II,7
10 %O 03 0,5 1 810
w
6 Construction
6.1 Material
The pipettes shall be made of translucent plastics material of chemical and physical properties suitable for
their intended use. The plastic material or its symbol in accordance with IS0 1043-I shall be marked on each
pipettes.
---------------------- Page: 4 ----------------------
@ IS0 IS0 12771:1997(E)
6.2 Graduation and figuring
The pipettes shall be provided with a graduation in accordance with IS0 8417, and figured accordingly.
Graduation and figuring shall be durable until the pipette has been used.
The pipettes shall be graduated from 0 at the top down to the lowest graduation line according to table 1. The
distance between the top of the pipette and the zero line shall be at least 90 mm for pipettes with a separate
suction tube and 75 mm minimum for pipettes without suction tube.
6.3 Design
6.3.1 Pipettes shall be straight. Any cross-section of a pipette taken in a plane perpendicular to the longitudinal
axis shall be reasonably circular.
6.3.2 The lower end of the pipette shall termin
...
SLOVENSKI STANDARD
SIST ISO 12771:1998
01-april-1998
3ODVWLþQDODERUDWRULMVNDRSUHPD6HURORãNHSLSHWH]DHQNUDWQRXSRUDER
Plastics laboratory ware -- Disposable serological pipettes
Matériel de laboratoire en plastique -- Pipettes sérologiques à usage unique
Ta slovenski standard je istoveten z: ISO 12771:1997
ICS:
71.040.20 Laboratorijska posoda in Laboratory ware and related
aparati apparatus
SIST ISO 12771:1998 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
SIST ISO 12771:1998
---------------------- Page: 2 ----------------------
SIST ISO 12771:1998
INTERNATIONAL
IS0
STANDARD
12771
First edition
1997-05-01
Plastics laboratory ware - Disposable
serological pipettes
Matkriel de laboratoire en plastique - Pipettes s6rologiques h usage
unique
Reference number
IS0 12771:1997(E)
---------------------- Page: 3 ----------------------
SIST ISO 12771:1998
IS0 12771 :1997(E)
Foreword
IS0 (the International Organization for Standardization) is a worldwide federation of national standards bodies
(IS0 member bodies). The work of preparing International Standards is normally carried out through IS0
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. IS0 collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are circulated to the member bodies for
voting. Publication as an International Standard requires approval by at least 75 % of the member bodies
casting a vote.
International Standard IS0 12771 was prepared by Technical Committee lSO/TC 48, Laboratory glassware and
related apparatus, Subcommittee SC 1, Volumetric instruments.
Annex A of this International Standard is for information only.
0 IS0 1997
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronic or mechanical, including photocopying and
microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case postale 56 l CH-1211 Geneve 20 l Switzerland
Internet central @ isoch
x.400 c=ch; a=400net; p=iso; o=isocs; s=central
Printed in Switzerland
ii
---------------------- Page: 4 ----------------------
SIST ISO 12771:1998
INTERNATIONAL STANDARD @ IS0 IS0 12771 :1997(E)
Plastics laboratory ware - Disposable serological pipettes
1
Scope
This International Standard specifies requirements for disposable plastics serological pipettes, gauged to
deliver, suitable for general laboratory purposes.
2 Normative references
The following standards contain provisions which, through references in this text, constitute provisions of this
International Standard. At the time of publication, the editions indicated were valid. All standards are subject to
revision, and parties to agreements based on this International Standard are encouraged to investigate the
possibility of applying the most recent editions of the standards indicated below. Members of IEC and IS0
maintain registers of currently valid International Standards.
IS0 1043-I : 1987, Plastics - Symbols - Part 1: Basic polymers and their special characteristics.
IS0 1769: 1975, Laboratory glassware - Pipettes - Colour coding.
IS0 8417:~,” Laboratory volumetric instruments - Principles of design and construction of disposable
volumetric articles .
Definitions
3
For the purposes of this International Standard, the following definitions apply.
3.1 disposable: Adjective used to describe serological pipettes which are intended to be used once only and
then discarded.
NOTE - Such pipettes will only be expected to provide their specified performance during the original operation.
3.2 accuracy (of a pipette): Closeness of agreement between the nominal volume and the true volume,
obtained by applying the test procedure specified in clause 9.1. It is quantified by the capacity deviation.
3.3 repeatability (of a number of pipettes): Closeness of agreement between the individual volumes obtained
by applying the test procedure specified in 9.2.
NOTE 1: Definitions and terms are in agreement with the “International Vocabulary of Basic and General Terms in
Metrology”. See IS0 3534-l for details.
NOTE 2: The definitions for accuracy and repeatability apply only in cases where the distributions are Gaussian.
4 Basis of adjustment
4.1 Unit of volume
The unit of volume is the cubic centimetre (cm”), for which the name millilitre (ml) may be used.
‘) To be published.
---------------------- Page: 5 ----------------------
SIST ISO 12771:1998
IS0 12771 :1997(E) 0 IS0
NOTE - The term millilitre (ml) is commonly used as a special name for the cubic centimetre (cm”), in accordance with the
International System of Units (Sl).
4.2 Reference temperature
The reference temperature, i.e. the temperature at which the pipette is intended to deliver its nominal volume
(nominal capacity), is 20 OC.
When the pipette is required for use in a country which has adopted a reference temperature of 27 OC (the
alternative specified in IS0 8417 for tropical use), this figure shall be substituted for 20 OC.
4.3 Adjustment
The pipettes shall be adjusted to deliver the required volume (i.e. the nominal volume or a part of it) with the
last drop to be blown out.
5 Dimensions and delivery times
The dimensions and delivery times shall be as shown in table 1.
The delivery time shall be determined with the pipette unplugged, and with distilled water at a temperature of
2OOC.
Table 1 - Capacity, subdivision, dimensions and delivery times
I I I I
Nominal Smallest Lowest External Wall Delivery
scale division graduation thickness
capacity
times
diameter
line at min.
min. max.
ml mm
ml ml
mm
S
015 0,Ol Of4 4,5 to
4,8 018
1 0,Ol 03 -
4,5to 5,0 03
1
1
OrI 03 4,5to 5,0 018 Of5 1 3,o
2 0,Ol
'13 5,4 to 6,7 03 or5 40
2 w ‘13 5,4 to 6,7 02 Of5 / 0
0,5 1 7,0
5 01 I 0 7,6 to IO,0 03
9,5to II,7
10 %O 03 0,5 1 810
w
6 Construction
6.1 Material
The pipettes shall be made of translucent plastics material of chemical and physical properties suitable for
their intended use. The plastic material or its symbol in accordance with IS0 1043-I shall be marked on each
pipettes.
---------------------- Page: 6 ----------------------
SIST ISO 12771:1998
@ IS0 IS0 12771:1997(E)
6.2 Graduation and figuring
The pipettes shall be provided with a graduation in acc
...
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