SIST EN ISO 9509:1998
(Main)Water quality - Method for assessing the inhibition of nitrification of activated sludge micro-organisms by chemicals and waste water (ISO 9509:1989)
Water quality - Method for assessing the inhibition of nitrification of activated sludge micro-organisms by chemicals and waste water (ISO 9509:1989)
Wasserbeschaffenheit - Verfahren zur Bestimmung der Nitrifikations-Hemmung von Mikroorganismen im Belebtschlamm durch Stoffe und Abwasser (ISO 9509:1989)
1.1 Diese Norm beschreibt ein Verfahren zur Bestimmung der Kurzzeit-Hemmwirkung von Stoffen auf nitrifizierende Bakterien in Belebtschlamm. Die Hemmwirkung wirdüber eine Untersuchungsdauer von 4 h bestimmt. 1.2 Das Verfahren ist geeignet für nitrifizierenden Belebtschlamm aus kommunalem Abwasser. Es können auch nitrifizierende Schlämme, die mit systhetischem Abwasser erhalten wurden, eingesetzt werden.
Qualité de l'eau - Méthode pour l'évaluation de l'effet inhibiteur sur la nitrification par des micro-organismes de boues activées par des produits chimiques ou des eaux résiduaires (ISO 9509:1989)
Les domaines d'application de la CEI 61008-1 et de la CEI 61009-1 s'appliquent avec les additions suivantes. La CEI 62423:2009 spécifie les exigences et les essais pour les DDR (dispositif de protection à courant différentiel résiduel) de Type B et de Type F. Les exigences et les essais donnés dans la présente norme complètent les exigences des dispositifs à courant différentiel résiduel de Type A. La présente norme ne peut être utilisée que conjointement à la CEI 61008-1 et à la CEI 61009-1. Les ID (interrupteur différentiel sans protection contre les surintensités incorporée) de Type F et les DD (interrupteur différentiel avec protection contre les surintensités incorporée) de Type F de fréquence assignée 50 Hz ou 60 Hz sont destinés à des installations dans lesquelles les convertisseurs de fréquence sont alimentés entre phase et neutre ou entre phase et conducteur milieu mis à la terre et sont aptes à assurer la protection en cas d'apparition de courant différentiel alternatif sinusoïdal à la fréquence assignée, de courant différentiel continu pulsé et de courant différentiel composé. Les ID de Type B et les DD de Type B sont aptes à assurer la protection en cas de courants différentiels résiduels alternatifs sinusoïdaux jusqu'à 1 000 Hz, de courants différentiels résiduels continus pulsés et de courants différentiels résiduels continus lissés. Cette deuxième édition annule et remplace la première édition publiée en 2007 dont elle constitue une révision technique. Les changements par rapport à la première édition sont les suivants:
- des exigences et essais ont été introduits pour le DDR de Type F;
- des exigences et essais ont été introduits pour le DDR à deux pôles de Type B;
- de nouvelles exigences et de nouveaux essais supplémentaires pour les DDR de Type B ont été introduits pour couvrir aussi les exigences et essais pour le Type F.
Le contenu du corrigendum de décembre 2011 a été pris en considération dans cet exemplaire.
Kakovost vode - Metoda za ocenjevanje zaviranja mikrobne nitrifikacije v aktivnem blatu zaradi delovanja kemikalij in odpadnih voda (ISO 9509:1989)
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 9509:1998
01-januar-1998
Kakovost vode - Metoda za ocenjevanje zaviranja mikrobne nitrifikacije v aktivnem
blatu zaradi delovanja kemikalij in odpadnih voda (ISO 9509:1989)
Water quality - Method for assessing the inhibition of nitrification of activated sludge
micro-organisms by chemicals and waste water (ISO 9509:1989)
Wasserbeschaffenheit - Verfahren zur Bestimmung der Nitrifikations-Hemmung von
Mikroorganismen im Belebtschlamm durch Stoffe und Abwasser (ISO 9509:1989)
Qualité de l'eau - Méthode pour l'évaluation de l'effet inhibiteur sur la nitrification par des
micro-organismes de boues activées par des produits chimiques ou des eaux résiduaires
(ISO 9509:1989)
Ta slovenski standard je istoveten z: EN ISO 9509:1995
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST EN ISO 9509:1998 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN ISO 9509:1998
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SIST EN ISO 9509:1998
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SIST EN ISO 9509:1998
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SIST EN ISO 9509:1998
INTERNATIONAL
ISO
9509
STANDARD
First edition
1989-10-15
Water quality - Method for assessing the
inhibition of nitrification of activated sludge
micro-organisms by chemicals and waste waters
Quakt! de l’eau - Methode pour l%valuation de l’effet inhibiteur SW Ia nitriikation
par des micro-organismes de boues activt!es par des produits chimiques ou des
eaux rkiduaires
Reference number
ISO 9509 : 1989 (EI
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SIST EN ISO 9509:1998
ISO 9509 : 1989 (El
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of
national Standards bodies (ISO member bedies). The work of preparing International
Standards is normally carried out through ISO technical committees. Esch member
body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, govern-
mental and non-governmental, in liaison with ISO, also take part in the work. ISO
collaborates closely with the International Electrotechnical Commission (IEC) on all
matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are circulated to
the member bodies for approval before their acceptance as International Standards by
the ISO Council. They are approved in accordance with ISO procedures requiring at
least 75 % approval by the member bodies voting.
International Standard ISO 9509 was prepared by Technical Committee ISO/TC 147
Water quality.
Annexes A and B form an integral part of this International Standard. Annex C is for in-
formation only.
0 ISO 1989
All rights reserved. No patt of this publication may be reproduced or utilized in any form or by any
means, electronie or mechanical, including photocopying and microfilm, without Permission in
writing from the publisher.
International Organization for Standardization
Case postale 56 l CH-121 1 Geneve 20 l Switzerland
Printed in Switzerland
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SIST EN ISO 9509:1998
INTERNATIONAL STANDARD ISO 9509 : 1989 (E)
Water quality - Method for assessing the inhibition of
nitrification of activated sludge micro-organisms by
chemicals and waste waters
WARNING - Activated sludges may contain pathogenic organisms, therefore take appropriate precautions when hand-
ling them.
Handle toxic test substances and those with unknown properties with care.
1 Scope were valid. All Standards are subject to revision, and Parties to
agreements based on this International Standard are encouraged
to investigate the possibility of applying the most recent editions
1.1 This International Standard specifies a method for
of the Standards indicated below. Members of IEC and ISO main-
assessing the short-term inhibitory effects of test substances
tain registers of currently valid International Standards.
on nitrifying bacteria in activated sludge. The inhibitory effect is
estimated over an exposure period of 4 h.
ISO 6107-1 : 1986, Water quality - Vocabulary - Part 1.
ISO 6107-3: 1985, Water quality - Vocabulary - Part 3.
1.2 The method is suitable for use with nitrifying activated
sludge derived from domestic sewage. lt is also possible to use
nitrifying sludges derived from synthetic sewage.
3 Definitions
1.3 Initially, the nitrifying activity of the activated sludge is
For the purposes of this International Standard, the following
verified by addition of a specific inhibitor (e.g. allylthiourea).
definitions apply.
If the nitrification rate (see 8.1) is within a suitable range for the
3.1 test Chemical: Pure chemicals, mixtures, Chemical pro-
test [2 to 6,5 milligrams of nitrogen per gram suspended solids
ducts and waste waters.
per hour (2 mg of N/(g-h) to 6,5 mg of N/(gmh)l, the sludge is
used directly; if not, adjustments are necessary (see clause 9).
3.2 activated sludge : Accumulated biological mass (floc)
produced in the treatment of waste water by the growth of
1.4 The method is applicable to non-volatile Chemical
bacteria and other micro-organisms in the presence of dissolved
substances which are soluble in water and also to waste
Oxygen. [ISO 6107-1 .l
waters. lt is possible to use insoluble substances, if care is
taken to ensure as much homogeneity as possible.
3.3 mixed liquor suspended solids (MLSS): The concen-
tration of solids, expressed in a specified dried form, in the
1.5 lt is important to stress that sludges from different
mixed liquor. [ISO 6107-3.1
sources respond differently to a given concentration of an in-
hibitor and this is probably due, at least in Part, to reaction be-
NOTE - In this International Standard, the MLSS are determined after
filtration of a known volume and drying at about 100 OC. They are ex-
tween the inhibitor and components of the sludge resulting in a
pressed in milligrams per litre or grams per litre.
partial nullifying of the toxic effect. Also, since the test lasts
only 4 h, it must be borne in mind that any inhibitory effects
may diminish, or increase, over a longer period, e.g. in the con- 3.4 EC 50: Concentration of test material giving a calculated
tinuous activated sludge System. or interpolated inhibition of nitrification of 50 % compared with
a control containing no test material.
2 Normative references
35 nitrification : The Oxidation of ammonium salts by
bacteria. Usually, the end product of such an Oxidation is
The following Standards contain provisions which, through
nitrate. [ISO 6107-11.
reference in this text, constitute provisions of this International
Standard. At the time of publication, the editions indicated
NOTE - Nitrites may be formed as intermediate products.
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SIST EN ISO 9509:1998
ISO 9509 : 1989 (El
6.5 Apparatus, necessary for analytical determination of
4 Principle
ammonia and oxidized nitrogen in Solution.
Performance of the test at a constant temperature, usually be-
tween 20 OC and 25 OC, in an atmosphere free from dust and
6.6 Filtration apparatus.
toxic vapours. Parallel aeration of a nitrifying sludge in the
presence and absence of test material and assessment of the
differente in concentration of oxidized nitrogen (nitrite N plus 6.7 Filter, made of glass fibre or Paper which does not
nitrate N) produced by the Oxidation of ammonium salts. release nitrogen.
Calculation of the inhibition of nitrification of activated sludge
micro-organisms by the test material.
7 Procedure
5 Reagents and materials
7.1 Use sludges with specific nitration rates between 2 mg of
N/(g.h) and 6,5 mg of N/(g-h). If the rate is outside this range,
5.1 Water, deionized or distilled.
it is essential to modify the procedure (sec clause 9).
5.2 Nitrifying activated sludge
7.2 Add equal volumes of washed nitrifying sludge (5.2) to a
series of 500 ml conical flasks (6.1) so that the final concentra-
Obtain a Portion of sludge from a nitrifying treatment plant
tion of mixed liquor suspended solids will be approximately
receiving domestic sewage or from a laboratory-scale plant
1 500 mg/l (sec table B.1).
treating domestic or synthetic sewage. Maintain the sludge in an
aerobic condition and preferably use within 24 h of collection.
Add 25 ml of medium (5.3) to each flask and add one of a range
Before use, centrifuge the sludge (e.g. 1 100 g during 5 min) and
of volumes (usually five) of test Solution (5.5) and sufficient
discard the supernatant liquid. Wash the residue with an equal
distilled water (5.1) to make the final volume up to 250 ml (see
volume of water (5.11, dilute the resulting mixture with ten times
annex B).
the volume of medium (5.31, re-centrifuge and again discard the
supernatant liquid. Finally, resuspend the sludge in an appropriate
Include a control flask with sludge, medium and water but no
volume of medium (5.3) to give the required concentration of
test substance, and a reference flask with sludge, medium,
mixed liquor suspended solids (e.g. 3 g/l) and aerate until use.
water and 2,5 ml of reference inhibitor (5.4) . If required ( as an
extra check), take a Sample of the control to determine the con-
5.3 Medium centration of ammonia.
Dissol
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