Milk and milk products - Determination of alkaline phosphatase activity - Part 2: Fluorimetric method for cheese (ISO 11816-2:2024)

ISO 11816-2:2016|IDF 155-2:2016 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in cheese.
This method is applicable to soft cheeses, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7).
The instrument can read activities in the supernatant up to 7 000 milliunits per litre (mU/l).

Milch und Milchprodukte - Bestimmung der Aktivität der alkalischen Phosphatase - Teil 2: Fluorimetrisches Verfahren für Käse (ISO 11816-2:2024)

Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 2: Méthode fluorimétrique pour le fromage (ISO 11816-2:2024)

L'ISO 11816-2 :2016|FIL 155-2 :2016 spécifie une méthode fluorimétrique de détermination de l'activité de la phosphatase alcaline (ALP, EC 3.1.3.1) dans le fromage.
La présente méthode est applicable aux fromages à pâte molle, pâte pressée non cuite et pâte pressée cuite, à condition que la moisissure ne soit présente que sur la surface du fromage et ne se soit pas propagée à l'intérieur, comme dans le cas des fromages veinés de bleu, par exemple. Pour les gros fromages à pâte pressée cuite, des conditions d'échantillonnage spécifiques s'appliquent (voir l'Article 7).
L'instrument permet de détecter des activités dans le surnageant de l'ordre de 7 000 milliunités par litre (mU/l).

Mleko in mlečni izdelki - Določanje aktivnosti alkalne fosfataze - 2. del: Fluorometrijska metoda za sir (ISO 11816-2:2024)

General Information

Status
Published
Public Enquiry End Date
27-Feb-2023
Publication Date
08-Feb-2024
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
02-Feb-2024
Due Date
08-Apr-2024
Completion Date
09-Feb-2024

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SLOVENSKI STANDARD
SIST EN ISO 11816-2:2024
01-marec-2024
Nadomešča:
SIST EN ISO 11816-2:2016
Mleko in mlečni proizvodi - Določanje aktivnosti alkalne fosfataze - 2. del:
Fluorometrijska metoda za sir (ISO 11816-2:2024)
Milk and milk products - Determination of alkaline phosphatase activity - Part 2:
Fluorimetric method for cheese (ISO 11816-2:2024)
Milch und Milchprodukte - Bestimmung der Aktivität der alkalischen Phosphatase - Teil
2: Fluorimetrisches Verfahren für Käse (ISO 11816-2:2024)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 2:
Méthode fluorimétrique pour le fromage (ISO 11816-2:2024)
Ta slovenski standard je istoveten z: EN ISO 11816-2:2024
ICS:
67.100.30 Sir Cheese
SIST EN ISO 11816-2:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN ISO 11816-2:2024

---------------------- Page: 2 ----------------------
SIST EN ISO 11816-2:2024


EN ISO 11816-2
EUROPEAN STANDARD

NORME EUROPÉENNE

January 2024
EUROPÄISCHE NORM
ICS 67.100.30 Supersedes EN ISO 11816-2:2016
English Version

Milk and milk products - Determination of alkaline
phosphatase activity - Part 2: Fluorimetric method for
cheese (ISO 11816-2:2024)
Lait et produits laitiers - Détermination de l'activité de Milch und Milchprodukte - Bestimmung der Aktivität
la phosphatase alcaline - Partie 2: Méthode der alkalischen Phosphatase - Teil 2: Fluorimetrisches
fluorimétrique pour le fromage (ISO 11816-2:2024) Verfahren für Käse (ISO 11816-2:2024)
This European Standard was approved by CEN on 29 March 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11816-2:2024 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN ISO 11816-2:2024
EN ISO 11816-2:2024 (E)
Contents Page
European foreword . 3

2

---------------------- Page: 4 ----------------------
SIST EN ISO 11816-2:2024
EN ISO 11816-2:2024 (E)
European foreword
This document (EN ISO 11816-2:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2024, and conflicting national standards shall be
withdrawn at the latest by July 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11816-2:2016.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
...

SLOVENSKI STANDARD
SIST EN ISO 11816-2:2024
01-marec-2024
Nadomešča:
SIST EN ISO 11816-2:2016
Mleko in mlečni izdelki - Določanje aktivnosti alkalne fosfataze - 2. del:
Fluorometrijska metoda za sir (ISO 11816-2:2024)
Milk and milk products - Determination of alkaline phosphatase activity - Part 2:
Fluorimetric method for cheese (ISO 11816-2:2024)
Milch und Milchprodukte - Bestimmung der Aktivität der alkalischen Phosphatase - Teil
2: Fluorimetrisches Verfahren für Käse (ISO 11816-2:2024)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 2:
Méthode fluorimétrique pour le fromage (ISO 11816-2:2024)
Ta slovenski standard je istoveten z: EN ISO 11816-2:2024
ICS:
67.100.30 Sir Cheese
SIST EN ISO 11816-2:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN ISO 11816-2:2024

---------------------- Page: 2 ----------------------
SIST EN ISO 11816-2:2024


EN ISO 11816-2
EUROPEAN STANDARD

NORME EUROPÉENNE

January 2024
EUROPÄISCHE NORM
ICS 67.100.30 Supersedes EN ISO 11816-2:2016
English Version

Milk and milk products - Determination of alkaline
phosphatase activity - Part 2: Fluorimetric method for
cheese (ISO 11816-2:2024)
Lait et produits laitiers - Détermination de l'activité de Milch und Milchprodukte - Bestimmung der Aktivität
la phosphatase alcaline - Partie 2: Méthode der alkalischen Phosphatase - Teil 2: Fluorimetrisches
fluorimétrique pour le fromage (ISO 11816-2:2024) Verfahren für Käse (ISO 11816-2:2024)
This European Standard was approved by CEN on 29 March 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11816-2:2024 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN ISO 11816-2:2024
EN ISO 11816-2:2024 (E)
Contents Page
European foreword . 3

2

---------------------- Page: 4 ----------------------
SIST EN ISO 11816-2:2024
EN ISO 11816-2:2024 (E)
European foreword
This document (EN ISO 11816-2:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2024, and conflicting national standards shall be
withdrawn at the latest by July 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11816-2:2016.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
U
...

SLOVENSKI STANDARD
oSIST prEN ISO 11816-2:2023
01-februar-2023
Mleko in mlečni proizvodi - Določanje aktivnosti alkalne fosfataze - 2. del:
Fluorometrijska metoda za sir (ISO/DIS 11816-2:2022)
Milk and milk products - Determination of alkaline phosphatase activity - Part 2:
Fluorimetric method for cheese (ISO/DIS 11816-2:2022)
Milch und Milchprodukte - Bestimmung der Aktivität der alkalischen Phosphatase - Teil
2: Fluorimetrisches Verfahren für Käse (ISO/DIS 11816-2:2022)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 2:
Méthode fluorimétrique pour le fromage (ISO/DIS 11816-2:2022)
Ta slovenski standard je istoveten z: prEN ISO 11816-2
ICS:
67.100.30 Sir Cheese
oSIST prEN ISO 11816-2:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN ISO 11816-2:2023

---------------------- Page: 2 ----------------------
oSIST prEN ISO 11816-2:2023
DRAFT INTERNATIONAL STANDARD
ISO/DIS 11816-2
ISO/TC 34/SC 5 Secretariat: NEN
Voting begins on: Voting terminates on:
2022-12-06 2023-02-28
Milk and milk products — Determination of alkaline
phosphatase activity —
Part 2:
Fluorimetric method for cheese
Lait et produits laitiers — Détermination de l'activité de la phosphatase alcaline —
Partie 2: Méthode fluorimétrique pour le fromage
ICS: 67.100.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 11816-2:2022(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2022

---------------------- Page: 3 ----------------------
oSIST prEN ISO 11816-2:2023
ISO/DIS 11816-2:2022(E)
DRAFT INTERNATIONAL STANDARD
ISO/DIS 11816-2
ISO/TC 34/SC 5 Secretariat: NEN
Voting begins on: Voting terminates on:

Milk and milk products — Determination of alkaline
phosphatase activity —
Part 2:
Fluorimetric method for cheese
Lait et produits laitiers — Détermination de l'activité de la phosphatase alcaline —
Partie 2: Méthode fluorimétrique pour le fromage
ICS: 67.100.30
This document is circulated as received from the committee secretariat.
COPYRIGHT PROTECTED DOCUMENT
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
© ISO 2022
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
NOT BE REFERRED TO AS AN INTERNATIONAL
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on STANDARD UNTIL PUBLISHED AS SUCH.
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
IN ADDITION TO THEIR EVALUATION AS
or ISO’s member body in the country of the requester. BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
ISO copyright office
USER PURPOSES, DRAFT INTERNATIONAL
CP 401 • Ch. de Blandonnet 8
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
CH-1214 Vernier, Geneva
POTENTIAL TO BECOME STANDARDS TO
Phone: +41 22 749 01 11
WHICH REFERENCE MAY BE MADE IN
Reference number
Email: copyright@iso.org
NATIONAL REGULATIONS.
Website: www.iso.org ISO/DIS 11816-2:2022(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
Published in Switzerland
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
ii
  © ISO 2022 – All rights reserved
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2022

---------------------- Page: 4 ----------------------
oSIST prEN ISO 11816-2:2023
ISO/DIS 11816-2:2022(E)
Contents Page
Forewords .iv
1 Scope . 1
2 Normative references .
...

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