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ASTM D5590-00

(Test Method)

Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated Four-Week Agar Plate Assay

Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated Four-Week Agar Plate Assay

SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of two or more paints or coating films to fungal growth.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
09-Nov-2000
ICS
87.040 - Paints and varnishes
Technical Committee
D01 - Paint and Related Coatings, Materials, and Applications
Drafting Committee
D01.28 - Biodeterioration
Current Stage
Ref Project

Relations

Replaces
ASTM D5590-94 - Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated Four-Week Agar Plate Assay
Effective Date
10-Nov-2000
Replaced By
ASTM D5590-00(2005) - Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated Four-Week Agar Plate Assay
Effective Date
01-Dec-2005
Referred By
ASTM E3152-23 - Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents
Effective Date
10-Nov-2000
Referred By
ASTM D4445-23 - Standard Test Method for Fungicides for Controlling Sapstain and Mold on Unseasoned Lumber (Laboratory Method)
Effective Date
10-Nov-2000
Referred By
ASTM D4708-19 - Standard Practice for Preparation of Uniform Free Films of Organic Coatings
Effective Date
10-Nov-2000
Referred By
ASTM C647-19 - Standard Guide to Properties and Tests of Mastics and Coating Finishes for Thermal Insulation
Effective Date
10-Nov-2000

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ASTM D5590-00 - Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated Four-Week Agar Plate AssayASTM D5590-00 - Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated Four-Week Agar Plate Assay
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ASTM D5590-00 - Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated Four-Week Agar Plate Assay
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Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:D5590–00
Standard Test Method for
Determining the Resistance of Paint Films and Related
Coatings to Fungal Defacement by Accelerated Four-Week
1
Agar Plate Assay
This standard is issued under the fixed designation D 5590; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
4
1. Scope Polymeric Materials to Fungi
1.1 This test method covers an accelerated method for
3. Summary of Test Method
determining the relative resistance of two or more paints or
3.1 This test method outlines a procedure to (1) prepare a
coating films to fungal growth.
suitable specimen for testing, ( 2) inoculate the specimen with
1.2 The values stated in SI units are to be regarded as the
the proper fungal species, ( 3) expose the inoculated samples
standard. The values given in parentheses are for information
under the appropriate conditions for growth, and (4) provide a
only.
schedule and guidelines for visual growth ratings. This test
1.3 This standard does not purport to address all of the
method is not designed to include all the necessary procedures
safety concerns, if any, associated with its use. It is the
to maintain the proper microbiological techniques required to
responsibility of the user of this standard to establish appro-
provide the most accurate results.
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
4. Significance and Use
4.1 Defacement of paint and coating films by fungal growth
2. Referenced Documents
(mold, mildew) is a common phenomenon, and defacement by
2.1 ASTM Standards:
algal growth can also occur under certain conditions. It is
D 822 Practice for Conducting Tests on Paint and Related
generally known that differences in the environment, lighting,
Coatings and Materials Using Filtered Open-Flame
2 temperature, humidity, substrate pH, and other factors in
Carbon-Arc Light and Water Exposure Apparatus
addition to the coating composition affect the susceptibility of
D 3273 Test Method for Resistance to Growth of Mold on
a given painted surface. This test method attempts to provide a
the Surface of Interior Coatings in an Environmental
2 meanstocomparativelyevaluatedifferentcoatingformulations
Chamber
for their relative performance under a given set of conditions.
D 3456 Practice for Determining by Exterior Exposure
It does not imply that a coating that resists growth under these
Tests the Susceptibility of Paint Films to Microbiological
2 conditions will necessarily resist growth in the actual applica-
Attack
tion.
D 4141 Practicefor ConductingAccelerated OutdoorExpo-
2
sure Tests of Coatings
NOTE 1—It is hoped that a ranking of relative performance would be
D 4587 Practice for Conducting Tests on Paint and Related similar to that ranked from outdoor exposures. However, this test method
should not be used as a replacement for exterior exposure (that is, Practice
Coatings and Materials Using a Fluorescent UV-
3
D 3456)sincemanyotherfactors,onlyafewofwhicharelistedwillaffect
Condensation Light- and Water-Exposure Apparatus
those results.
D 5031 Practice for ConductingTests on Paints and Related
NOTE 2—Several companies have reported reasonable correlation of
Coatings and Materials Using Enclosed Carbon-Arc Light
resultsfromthistestwithactualusewhentestingfilm-forming,pigmented
2
and Water Exposure Apparatus
coatings. Round-robin testing of this test method versus exterior exposure
G 21 Practice for Determining Resistance of Synthetic
is planned.
4.2 Familiarity with microbiological techniques is required.
This test method should not be used by persons without at least
1
basic microbiological training.
This test method is under the jurisdiction of ASTM Committee D01 on Paint
and Related Coatings, Materials, andApplications and is the direct responsibility of
5. Apparatus and Materials
Subcommittee D01.28 Biodeterioration.
Current edition approved Nov. 10, 2000. Published January 2001. Originally
5.1 Balance, capable of weighing to 0.10 g.
published as D 5590–94. Last previous edition D 5590–94.
2
Annual Book of ASTM Standards, Vol 06.01.
3
Discontinued 2000; see 1999 Annual Book of ASTM Standards, Vol 06.01.
4
Annual Book of ASTM Standards, Vol 014.04.
Copyright ©ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA19428-2959, United States.
1

---------------------- Page: 1 ----------------------
D5590
7 8
5.2 Incubator, or other device capable of maintaining a
Fungi ATCC # MYCO #
Aspergillus niger 6275 .
constant temperature between 25 and 30°C, relative humidity
Penicillium funiculosum 11797 391
of#85 %.
7
A
...

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