ASTM D7910-14(2021)
(Practice)Standard Practice for Collection of Fungal Material From Surfaces by Tape Lift
Standard Practice for Collection of Fungal Material From Surfaces by Tape Lift
SIGNIFICANCE AND USE
4.1 This practice defines a consistent procedure for collecting surface material using clear, transparent, single sided adhesive collection medium, typically tape (also known as tape lift).
4.2 A tape lift sample collected according to this practice is intended to be used to assess the material present at one specific location on a surface for fungal content.
4.3 A tape lift sample collected from a point of interest can be used for qualitative analysis or to quantify fungal material per sample or per unit area. Note that the recovery efficiency of material from the surface sampled is unknown and a likely source of uncertainty for quantitative analyses.
4.4 A tape lift sample collected according to this practice can be analyzed by direct microscopy.
4.5 This practice may help supplement consistency in mold sampling during an indoor air quality investigation.
SCOPE
1.1 This practice describes the protocols for collection of surface samples using tape lifts and their delivery to the laboratory.
1.2 The purpose of this practice is to support the field investigator in differentiating fungal materials from non-fungal material such as scuffs, soot deposits, stains, pigments, dust, efflorescence, adhesives, and water stains.
1.3 The samples collected by this practice are appropriate for either qualitative or quantitative analysis by direct microscopy.
1.4 This practice does not address building occupant exposures, or occupant health risks.
1.5 This practice does not address the development of a formal hypothesis or the establishment of sampling objectives.
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
General Information
Relations
Standards Content (Sample)
This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D7910 − 14 (Reapproved 2021)
Standard Practice for
Collection of Fungal Material From Surfaces by Tape Lift
This standard is issued under the fixed designation D7910; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2. Referenced Documents
1.1 This practice describes the protocols for collection of 2.1 ASTM Standards:
surface samples using tape lifts and their delivery to the D1356 Terminology Relating to Sampling and Analysis of
laboratory.
Atmospheres
D4840 Guide for Sample Chain-of-Custody Procedures
1.2 The purpose of this practice is to support the field
D6044 Guide for Representative Sampling for Management
investigator in differentiating fungal materials from non-fungal
of Waste and Contaminated Media
material such as scuffs, soot deposits, stains, pigments, dust,
efflorescence, adhesives, and water stains.
3. Terminology
1.3 The samples collected by this practice are appropriate
3.1 Definitions—For terminology not defined herein, refer
for either qualitative or quantitative analysis by direct micros-
to Terminology D1356.
copy.
3.2 Definitions of Terms Specific to This Standard:
1.4 This practice does not address building occupant
3.2.1 area (surface, sampled), n—a defined, measured ex-
exposures, or occupant health risks.
tent of surface sampled.
1.5 This practice does not address the development of a
3.2.2 chain of custody (COC), n—a document that provides
formal hypothesis or the establishment of sampling objectives.
for the traceable transfer of field samples to the analytical
1.6 The values stated in SI units are to be regarded as
laboratory. It may or may not be combined with the field data
standard. No other units of measurement are included in this
sheet. D4840
standard.
3.2.3 direct microscopy analysis, n—act of assessing micro-
1.7 This standard does not purport to address all of the
organisms (if present) using an optical compound microscope.
safety concerns, if any, associated with its use. It is the
3.2.4 field data sheet, n—a record of varying names that
responsibility of the user of this standard to establish appro-
provides a reference document for information directly related
priate safety, health, and environmental practices and deter-
to the sample collection event, including pre- and post-
mine the applicability of regulatory limitations prior to use.
calibration data.
1.8 This international standard was developed in accor-
3.2.5 fungal material, n—fungal spores, hyphae, and repro-
dance with internationally recognized principles on standard-
ductive structures.
ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom-
3.2.6 fungal structure (sing.), n—collective term for frag-
mendations issued by the World Trade Organization Technical
ments or groups of fragments from fungi, including but not
Barriers to Trade (TBT) Committee.
limited to conidia, conidiophores, hyphae, and spores.
3.2.7 fungi (pl.), n—eukaryotic, heterotrophic, absorptive
organisms that usually develop a rather diffuse, branched,
tubular body (that is, network of hyphae) and usually repro-
duce by means of spores. The terms ‘mold’and ‘mildew’are
frequently used by laypersons when referring to various fungal
colonization.
This practice is under the jurisdiction ofASTM Committee D22 on Air Quality
and is the direct responsibility of Subcommittee D22.08 on Assessment, Sampling, For referenced ASTM standards, visit the ASTM website, www.astm.org, or
and Analysis of Microorganisms. contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Current edition approved Sept. 1, 2021. Published September 2021. Originally Standards volume information, refer to the standard’s Document Summary page on
approved in 2014. Last previous edition approved in 2014 as D7910 – 14. DOI: the ASTM website.
10.1520/D7910-14R21. Kendrick, B., The Fifth Kingdom, Focus Publishing / R. Pullins & Co, 2008.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
D7910 − 14 (2021)
3.2.8 sample, n—a portion of material that is taken for 6.3 For adhesive tape sampling, perform the following
testing or record purposes. D6044 steps:
6.3.1 Removeanddiscardtapethathasbeenexposed(many
4. Significance and Use typical dispensers leave an area of tape exposed between the
roll and the cutter edge).
4.1 This practice defines a consistent procedure for collect-
6.3.2 Remove or dispense enough unexposed adhesive tape
ing surface material using clear, transparent, single sided
to take a surface sample. A tape strip 2.5 to 5.0 cm long is
adhesivecollectionmedium,typicallytape(alsoknownastape
typically enough and can easily fit on a microscope slide.
lift).
NOTE 4—For ease of handling tape, form a tab at one end of the tape
4.2 Atape lift sample collected according to this practice is
strip by folding the tape onto itself. The tab acts as a handle on the
intended to be used to assess the material present at one
adhesive tape.
specific location on a surface for fungal content.
6.3.3 Hold adhesive tape strip at edge (tab) of one end.
4.3 A tape lift sample collected from a point of interest can
NOTE 5—Ensure that tape adhesive surface does not make contact with
be used for qualitative analysis or to q
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