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ASTM E258-07(2015)

(Test Method)

Standard Test Method for Total Nitrogen in Organic Materials by Modified Kjeldahl Method (Withdrawn 2019)

Standard Test Method for Total Nitrogen in Organic Materials by Modified Kjeldahl Method (Withdrawn 2019)

SIGNIFICANCE AND USE
4.1 This test method may be used to determine the total nitrogen content of certain unknown organic samples, or to assay known nitrogen containing organic compounds.  
4.2 This test method may be used on organic materials in which the complete conversion of nitrogen to ammonium sulfate can be accomplished by digestion in a mixture of sulfuric acid, potassium sulfate, and mercuric oxide. It cannot be used on materials containing N–O, N–N linkages.  
4.3 This test method assumes that ammonia can be quantitatively measured by distillation from an alkaline solution into a measured volume of standard acid.
SCOPE
1.1 This test method covers the determination of total nitrogen in nitrogen-containing organic compounds. This test method is not applicable for use on materials containing N-O, N-N linkages.
1.2 The values stated in SI units are to be regarded as standard. The values given in parentheses are for information only.  
1.3  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Note 1: Another method of restricted application is given in Test Method D1013.
WITHDRAWN RATIONALE
This test method covers the determination of total nitrogen in nitrogen-containing organic compounds. This test method is not applicable for use on materials containing N-O, N-N linkages.
Formerly under the jurisdiction of Committee D16 on Aromatic, Industrial, Specialty and Related Chemicals, this test method was withdrawn in November 2019. This standard is being withdrawn without replacement due to its limited use by industry and it is no longer in line with D16 guidelines.

General Information

Status
Withdrawn
Publication Date
31-May-2015
Withdrawal Date
17-Nov-2019
ICS
71.080.30 - Organic nitrogen compounds
Technical Committee
D16 - Aromatic, Industrial, Specialty and Related Chemicals
Drafting Committee
D16.04 - Instrumental Analysis
Current Stage
Ref Project

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ASTM E258-07(2015) - Standard Test Method for Total Nitrogen in Organic Materials by Modified Kjeldahl Method (Withdrawn 2019)ASTM E258-07(2015) - Standard Test Method for Total Nitrogen in Organic Materials by Modified Kjeldahl Method (Withdrawn 2019)
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ASTM E258-07(2015) - Standard Test Method for Total Nitrogen in Organic Materials by Modified Kjeldahl Method (Withdrawn 2019)
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E258 −07 (Reapproved 2015)
Standard Test Method for
Total Nitrogen in Organic Materials by Modified Kjeldahl
1
Method
This standard is issued under the fixed designation E258; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the U.S. Department of Defense.
1. Scope* E200 Practice for Preparation, Standardization, and Storage
of Standard and Reagent Solutions for ChemicalAnalysis
1.1 This test method covers the determination of total
nitrogen in nitrogen-containing organic compounds. This test
3. Summary of Test Method
method is not applicable for use on materials containing N-O,
3.1 The sample is digested in a mixture of concentrated
N-N linkages.
sulfuric acid, potassium sulfate, and mercuric oxide. The
1.2 The values stated in SI units are to be regarded as
amounts of organic matter, potassium sulfate, and sulfuric acid
standard. The values given in parentheses are for information
present during the digestion step are critical. The organic
only.
material is oxidized and the nitrogen converted to ammonium
1.3 This standard does not purport to address all of the
sulfate. Sodium sulfide is added to the digested mixture to
safety concerns, if any, associated with its use. It is the
precipitate the mercury after which the solution is made
responsibility of the user of this standard to establish appro-
strongly alkaline with sodium hydroxide solution and the
priate safety, health, and environmental practices and deter-
ammoniawhichisliberatedisdistilledintoameasuredvolume
mine the applicability of regulatory limitations prior to use.
of standard acid. The amount of acid neutralized by the
ammonia is determined by titrating the excess acid with
NOTE 1—Another method of restricted application is given in Test
standard sodium hydroxide solution.
Method D1013.
1.4 This international standard was developed in accor-
4. Significance and Use
dance with internationally recognized principles on standard-
4.1 This test method may be used to determine the total
ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom- nitrogen content of certain unknown organic samples, or to
assay known nitrogen containing organic compounds.
mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee.
4.2 This test method may be used on organic materials in
which the complete conversion of nitrogen to ammonium
2. Referenced Documents
sulfate can be accomplished by digestion in a mixture of
2
2.1 ASTM Standards:
sulfuric acid, potassium sulfate, and mercuric oxide. It cannot
D1013 Test Method for Determining Total Nitrogen in
be used on materials containing N–O, N–N linkages.
3
Resins and Plastics (Withdrawn 2007)
4.3 This test method assumes that ammonia can be quanti-
D1193 Specification for Reagent Water
tatively measured by distillation from an alkaline solution into
a measured volume of standard acid.
1
This test method is under the jurisdiction of ASTM Committee D16 on
5. Apparatus
Aromatic, Industrial, Specialty and Related Chemicals and is the direct responsi-
bility of Subcommittee D16.04 on Instrumental Analysis.
5.1 Kjeldahl Flasks of moderately thick, well-annealed
This test method has been adopted from Method 2.054 of the Official Methods
borosilicate glass for digestion and distillation, 500 or 800-mL
ofAnalysis of theAssociation of OfficialAgricultural Chemists, 13th Edition, 1980.
capacity.
Current edition approved June 1, 2015. Published June 2015. Originally
approved in 1965. Last previous edition approved in 2007 as E258 – 07. DOI:
5.2 Connecting Bulbs of theWillits-John type, or a scrubber
10.1520/E0258-07R15.
2 bulb equally effective in preventing mechanical carry-over of
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
the contents of the distillation flask to the condenser (Note 2).
Standards volume information, refer to the standard’s Document Summary page on
NOTE 2—The usual flask and connecting bulb commercially available
the ASTM website.
3
The last approved version of this historical standard is referenced on are made to be assembled by means of a rubber stopper. To minimize
www.astm.org. lacerations from broken flasks while assembling the apparatus, it is
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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