ASTM D4783-98a
(Test Method)Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi
Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi
SCOPE
1.1 These test methods cover the determination of the resistance of liquid adhesive preparations to microbial attack in the container by challenging adhesive specimens with cultures of bacteria, yeast, or fungi, and checking for their ability to return to sterility. These test methods return qualitative results.
1.2 The values stated in SI units are to be regarded as the standard. The values in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. These test methods are designed to be used by persons trained in correct microbiological technique. Specific precautionary statements are given in Section 8.
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or
withdrawn. Contact ASTM International (www.astm.org) for the latest information.
Designation: D 4783 – 98a
AMERICAN SOCIETY FOR TESTING AND MATERIALS
100 Barr Harbor Dr., West Conshohocken, PA 19428
Reprinted from the Annual Book of ASTM Standards. Copyright ASTM
Standard Test Methods for
Resistance of Adhesive Preparations in Container to Attack
1
by Bacteria, Yeast, and Fungi
This standard is issued under the fixed designation D 4783; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
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1. Scope Cosmetics Preservation, Method 38
1.1 These test methods cover the determination of the
3. Terminology
resistance of liquid adhesive preparations to microbial attack in
3.1 Definitions:
the container by challenging adhesive specimens with cultures
3.1.1 resistance, n—as related to bacteria, yeast, or fungi,
of bacteria, yeast, or fungi, and checking for their ability to
the power or capacity to ward off growth.
return to sterility.
3.2 Definitions of Terms Specific to This Standard:
1.2 The values stated in SI units are to be regarded as the
3.2.1 adhesive preparation—the adhesive as packaged for
standard. The values in parentheses are for information only.
distribution, storage, and use.
1.3 This standard does not purport to address all of the
3.3 Abbreviations:
safety concerns, if any, associated with its use. It is the
3.3.1 PBS—phosphate buffered saline.
responsibility of the user of this standard to establish appro-
3.3.2 PDA—potato dextrose agar.
priate safety and health practices and determine the applica-
3.3.3 YMPG—yeast malt peptone glucose (agar).
bility of regulatory limitations prior to use. These test methods
3.4 For definitions of other terms, see Terminology D 907.
are designed to be used by persons trained in correct micro-
biological technique. Specific precautionary statements are
4. Summary of Test Methods
given in Section 8.
4.1 The adhesive specimen is challenged by inoculation
with a culture of bacteria, yeast, or fungi, which may be a
2. Referenced Documents
single species or a mixed culture of several species, following
2.1 ASTM Standards:
2 the guidelines given in Note 6. The inoculated adhesive
D 907 Terminology of Adhesives
specimen is stored at 21 to 27°C (70 to 80°F) for 7 days, during
D 4299 Test Methods for Effect of Bacterial Contamination
which time cultures (streak plates) are made at preset intervals.
on Performance of Adhesive Preparations and Adhesive
3
See Note 2. At any point in the series of challenges, if the
Films
inoculated specimen shows microbial growth on the streak
D 4300 Test Methods for Ability of Adhesive Films to
2
plates made during the week following the challenge (indicat-
Support or Resist the Growth of Fungi
ing that it has not returned to sterility), the test is discontinued,
E 640 Test Method for Preservatives in Water-Containing
4
and the sample is reported as not resistant to attack in the
Cosmetics
container by the species or combination of species used as the
NOTE 1—Test Method E 640 is under the jurisdiction of ASTM
inoculum. If the cultures show no growth, the test is repeated
Committee E-35 on Pesticides. The procedure in this method outlines a
with up to four challenges. If the specimen tests out as sterile
serial dilution method of determining plate count using a pour plate
following the fourth challenge, it is reported to be resistant to
technique.
attack in the container by the species or combination of species
2.2 TAPPI Method:
of bacteria, fungi, or yeast used as the inoculum. At the
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T 487 Fungus Resistance of Paper and Paperboard
discretion of the biological laboratory, the test may be discon-
2.3 CSMA:
tinued after the second or third challenge. See Section 16 for
further interpretation.
1
4.2 The time necessary to kill is determined by noting the
These test methods are under the jurisdiction of ASTM Committee D-14 on
Adhesives and are the direct responsibility of Subcommittee D14.30 on Wood
earliest streak plate to read sterile. If the 4-h plate is positive
Adhesives.
and the 24-h plate is negative, the kill time could be narrowed
Current edition approved Sept. 10, 1998. Published March 1999. Originally
down further by repeating the challenge and making streak
published as D 4783 – 88. Last previous edition D 4783 – 98.
2
Annual Book of ASTM Standards, Vol 15.06. plates at intervals of 4, 8, 12, and 24 h following the challenge.
3
Discontinued, see 1989 Annual Book of ASTM Standards, Vol 15.06.
4
Annual Book of ASTM Standards, Vol 11.05.
5 6
Available from TAPPI, P.O. Box 105113, Atlanta, GA 30348. This method is the same as Test Meth
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