ASTM D8068-19
(Practice)Standard Practice for Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction Systems
Standard Practice for Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction Systems
SIGNIFICANCE AND USE
4.1 This practice is intended for the collection of airborne particles on agar plates using inertial impaction for the purpose of culturing fungi or bacteria.
4.2 This practice is valuable when species level identification or quantity of culturable aerosolized fungi and bacteria are important factors for the indoor air quality investigation.
4.3 It is the responsibility of the user to assure that they are in compliance with all local, state, and federal regulations governing the inspection of buildings for fungal and bacterial colonization and the collection of associated samples.
4.4 This practice is intended to provide the user with a basic understanding of the equipment, materials, and instructions necessary to effectively collect air samples on agar plates using an inertial impactor.
4.5 This practice is intended to minimize systematic sampling variations between different data sets.
SCOPE
1.1 The purpose of this practice is to describe procedures for the collection of culturable airborne fungal spores or fragments or bacteria on agar plates using inertial impaction sampling techniques.
1.2 This practice does not include collection of culturable fungi or bacteria by devices not using agar plates.
1.3 This practice presumes that the user has a fundamental understanding of field investigative techniques related to the scientific process, and sampling plan development and implementation. It is important to establish the related hypothesis to be tested and the supporting analytical methodology needed in order to identify the sampling media to be used and the laboratory conditions for analysis.
1.4 This practice does not address the development of a formal hypothesis or the establishment of appropriate and defensible investigation and sampling objectives. It is presumed the investigator has the experience and knowledge base to address these issues.
1.5 This practice does not provide the user sufficient information to allow for interpretation of the analytical results from sample collection. It is the user's responsibility to seek or obtain the information and knowledge necessary to interpret the sample results reported by the laboratory.
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
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Standards Content (Sample)
This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D8068 − 19
Standard Practice for
Collection of Culturable Airborne Fungi or Bacteria on Agar
1
Plates by Inertial Impaction Systems
This standard is issued under the fixed designation D8068; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
This practice was introduced to bring uniformity to the process of sample collection for culturable
fungi or bacteria using inertial impaction samplers for analysis by culture and optical microscopy.
1. Scope responsibility of the user of this standard to establish appro-
priate safety, health, and environmental practices and deter-
1.1 Thepurposeofthispracticeistodescribeproceduresfor
mine the applicability of regulatory limitations prior to use.
the collection of culturable airborne fungal spores or fragments
1.8 This international standard was developed in accor-
or bacteria on agar plates using inertial impaction sampling
dance with internationally recognized principles on standard-
techniques.
ization established in the Decision on Principles for the
1.2 This practice does not include collection of culturable
Development of International Standards, Guides and Recom-
fungi or bacteria by devices not using agar plates.
mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee.
1.3 This practice presumes that the user has a fundamental
understanding of field investigative techniques related to the
2. Referenced Documents
scientific process, and sampling plan development and imple-
2
mentation. It is important to establish the related hypothesis to
2.1 ASTM Standards:
be tested and the supporting analytical methodology needed in
D1356 Terminology Relating to Sampling and Analysis of
order to identify the sampling media to be used and the
Atmospheres
laboratory conditions for analysis.
D3195/D3195M Practice for Rotameter Calibration
D6044 Guide for Representative Sampling for Management
1.4 This practice does not address the development of a
of Waste and Contaminated Media
formal hypothesis or the establishment of appropriate and
F1671 Test Method for Resistance of Materials Used in
defensible investigation and sampling objectives. It is pre-
Protective Clothing to Penetration by Blood-Borne Patho-
sumed the investigator has the experience and knowledge base
gens Using Phi-X174 Bacteriophage Penetration as a Test
to address these issues.
System
1.5 This practice does not provide the user sufficient infor-
mation to allow for interpretation of the analytical results from
3. Terminology
sample collection. It is the user’s responsibility to seek or
3.1 Definitions—For definitions of terms used in this
obtain the information and knowledge necessary to interpret
practice, see Terminology D1356.
the sample results reported by the laboratory.
3.1.1 agar, n—a semisolid culture medium used to support
1.6 The values stated in SI units are to be regarded as
the growth of bacteria and other micro-organisms. F1671
standard. No other units of measurement are included in this
3.1.2 sample, n—a portion of a population.
standard.
3.1.2.1 Discussion—A portion of material that is taken for
1.7 This standard does not purport to address all of the
testing or record purposes. D6044
safety concerns, if any, associated with its use. It is the
3.1.3 sample, representative, n—a sample collected in such
a manner that it reflects the characteristics of interest of a
1
This practice is under the jurisdiction ofASTM Committee D22 on Air Quality
and is the direct responsibility of Subcommittee D22.08 on Assessment, Sampling,
2
and Analysis of Microorganisms. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Current edition approved March 1, 2019. Published March 2019. Originally contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
approved in 2018. Last previous edition approved in 2018 as D8068 – 18. DOI: Standards volume information, refer to the standard’s Document Summary page on
10.1520/D8068-19. the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
D8068 − 19
population (as defined) from which it is collected. 5. Preparation of Sampling Equipment
adapted from D6044
5.1 Equipment List:
3.1.3.1 Discussion—A population in the context of this
5.1.1 Sampling Train—The combination of components
standard r
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: D8068 − 18 D8068 − 19
Standard Practice for
Collection of Culturable Airborne Fungi or Bacteria on Agar
1
Plates by Inertial Impaction Systems
This standard is issued under the fixed designation D8068; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
This practice was introduced to bring uniformity to the process of sample collection for culturable
fungi or bacteria using inertial impaction samplers for analysis by culture and optical microscopy.
1. Scope
1.1 The purpose of this practice is to describe procedures for the collection of culturable airborne fungal spores or fragments
or bacteria on agar plates using inertial impaction sampling techniques.
1.2 This practice does not include collection of culturable fungi or bacteria by devices not using agar plates.
1.3 This practice presumes that the user has a fundamental understanding of field investigative techniques related to the
scientific process, and sampling plan development and implementation. It is important to establish the related hypothesis to be
tested and the supporting analytical methodology needed in order to identify the sampling media to be used and the laboratory
conditions for analysis.
1.4 This practice does not address the development of a formal hypothesis or the establishment of appropriate and defensible
investigation and sampling objectives. It is presumed the investigator has the experience and knowledge base to address these
issues.
1.5 This practice does not provide the user sufficient information to allow for interpretation of the analytical results from sample
collection. It is the user’s responsibility to seek or obtain the information and knowledge necessary to interpret the sample results
reported by the laboratory.
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of
regulatory limitations prior to use.
1.8 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
D1356 Terminology Relating to Sampling and Analysis of Atmospheres
D3195/D3195M Practice for Rotameter Calibration
D6044 Guide for Representative Sampling for Management of Waste and Contaminated Media
D3195/D3195M Practice for Rotameter Calibration
E2589 Terminology Relating to Nonsieving Methods of Powder Characterization
1
This practice is under the jurisdiction of ASTM Committee D22 on Air Quality and is the direct responsibility of Subcommittee D22.08 on Assessment, Sampling, and
Analysis of Microorganisms.
Current edition approved Nov. 1, 2018March 1, 2019. Published November 2018March 2019. Originally approved in 2018. Last previous edition approved in 2018 as
D8068 – 18. DOI: 10.1520/D8068-18.10.1520/D8068-19.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
D8068 − 19
F1671 Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using
Phi-X174 Bacteriophage Penetration as a Test System
3. Terminology
3.1 Definitions—For definitions of terms used in this practice, see Terminology D1356.
3.1.1 agar, n—a semisolid culture medium used to support the growth of bacteria and other micro-organisms. F1671
3.1.2 sample, n—a portion of a population.
3.1.2.1 Discussion—
A portion of material that is taken for testing or record purposes. D6044
3.1.3 sample, representative, n—a sample collected in such a manner that
...
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