ASTM D6731-01(2011)
(Test Method)Standard Test Method for Determining the Aerobic, Aquatic Biodegradability of Lubricants or Lubricant Components in a Closed Respirometer
Standard Test Method for Determining the Aerobic, Aquatic Biodegradability of Lubricants or Lubricant Components in a Closed Respirometer
SIGNIFICANCE AND USE
Results from this test method suggest the degree of aerobic, aquatic biodegradation of a lubricant or lubricant component. The rate and extent of oxygen consumption is measured upon exposure of the test material to an inoculum within the confines of a controlled laboratory setting. Test materials which achieve a high degree of biodegradation in this test may be assumed to easily biodegrade in many aerobic aquatic environments.
Because of the stringency of this test method, low results do not necessarily mean that the test material is not biodegradable under environmental conditions, but indicate that further testing is necessary to establish biodegradability.
If the pH value at the end of the test is outside the range from 6 to 8 and if the percentage degradation of the test material is less than 50 %, it is advisable to repeat the test with a lower concentration of the test material or a higher concentration of the buffer solution, or both.
A reference or control material known to biodegrade under the conditions of this test method is necessary in order to verify the activity of the inoculum. The test must be regarded as invalid and shall be repeated using a fresh inoculum if the reference material does not demonstrate biodegradation to the extent of >60 % of the ThO2 within 28 days.
Information on the toxicity of the test material to the inoculum may be useful in the interpretation of low biodegradation results. Toxicity of the test material to the inoculum may be evaluated by testing the test material in combination with the reference material in inhibition control systems. If an inhibition control is included, the test material is assumed to be inhibiting if the degradation percentage of the reference material is lower than 40 % (ISO 8192:1986). In this case, it is advisable to repeat the test with lower concentrations of the test material.
Total oxygen utilization in the blank at the end of the test exceeding 60 mg O2/L invalidates the test.
The wat...
SCOPE
1.1 This test method covers a procedure for determining the degree of biodegradability of lubricants or their components in an aerobic aqueous medium on exposure to an inoculum under controlled laboratory conditions. This test method is an ultimate biodegradation test that measures oxygen demand in a closed respirometer.
1.2 This test method is suitable for evaluating the biodegradation of volatile as well as nonvolatile lubricants or lubricant components.
1.3 This test method is applicable to lubricants and lubricant components which are not toxic and not inhibitory to the test microorganisms at the test concentration.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazards are given in Section 10.
General Information
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D6731 − 01 (Reapproved 2011)
Standard Test Method for
Determining the Aerobic, Aquatic Biodegradability of
Lubricants or Lubricant Components in a Closed
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Respirometer
This standard is issued under the fixed designation D6731; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope D4447 Guide for Disposal of Laboratory Chemicals and
Samples
1.1 This test method covers a procedure for determining the
D6384 Terminology Relating to Biodegradability and Eco-
degree of biodegradability of lubricants or their components in
toxicity of Lubricants
an aerobic aqueous medium on exposure to an inoculum under
E943 Terminology Relating to Biological Effects and Envi-
controlled laboratory conditions. This test method is an ulti-
ronmental Fate
mate biodegradation test that measures oxygen demand in a
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2.2 ISO Standards:
closed respirometer.
ISO 4259:1992(E) Petroleum Products–Determination and
1.2 This test method is suitable for evaluating the biodeg-
Application of Precision Data in Relation to Methods of
radation of volatile as well as nonvolatile lubricants or lubri-
Test
cant components.
ISO 6107-2:1997 Water Quality–Vocabulary–Part 2
1.3 Thistestmethodisapplicabletolubricantsandlubricant ISO 8192:1986 Water Quality–Test for Inhibition of Oxygen
components which are not toxic and not inhibitory to the test
Consumption by Activated Sludge
microorganisms at the test concentration. ISO 9408:1999 Water Quality–Evaluation of UltimateAero-
bic Biodegradability of Organic Compounds in Aqueous
1.4 The values stated in SI units are to be regarded as
Medium by Determination of Oxygen Demand in a
standard. No other units of measurement are included in this
Closed Respirometer
standard.
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2.3 OECD Standards:
1.5 This standard does not purport to address all of the
OECD 301F:1992 Ready Biodegradability-Manometric Re-
safety concerns, if any, associated with its use. It is the
spirometry
responsibility of the user of this standard to establish appro-
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2.4 APHA Standards:
priate safety and health practices and determine the applica-
2540B Total Solids Dried at 103-105°C
bilityofregulatorylimitationspriortouse.Specifichazardsare
9215 Heterotrophic Plate Count
given in Section 10.
3. Terminology
2. Referenced Documents
2 3.1 Definitions:
2.1 ASTM Standards:
3.1.1 Definitions of terms applicable to this test method
D1129 Terminology Relating to Water
appear in the Compilation of ASTM Standard Definitions and
D1193 Specification for Reagent Water
the following terminology standards: D1129, D4175, D6384,
D1293 Test Methods for pH of Water
E943, and ISO 6107-2:1997.
D4175 Terminology Relating to Petroleum, Petroleum
3.1.2 activated sludge, n—the precipitated solid matter,
Products, and Lubricants
consisting mainly of bacteria and other aquatic
microorganisms, that is produced at a domestic wastewater
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This test method is under the jurisdiction of ASTM Committee D02 on
Petroleum Products, Liquid Fuels, and Lubricantsand is the direct responsibility of
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Subcommittee D02.12 on Environmental Standards for Lubricants. Available fromAmerican National Standards Institute (ANSI), 25 W. 43rd St.,
Current edition approved May 1, 2011. Published May 2011. Originally 4th Floor, New York, NY 10036, http://www.ansi.org.
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approved in 2001. Last previous edition approved in 2005 as D6731–01 (2005). Available from Organisation for Economic Cooperation and Development
DOI: 10.1520/D6731-01R11. (OECD), 2 rue André Pascal, F-75775, Paris Cedex 16, France, http://
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For referenced ASTM standards, visit the ASTM website, www.astm.org, or www.oecd.org.
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contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM From Standard Methods for the Examination of Water and Wastewater, latest
Standards volume information, refer to the standard’s Document Summary page on edition. Available from the American Public Health Assoc., 1015 18th St., NW,
the ASTM website. Washington, DC 20036.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
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D6731 − 01 (2011)
treatment plant and is used primarily in secondary sewage defined aquatic medium and measures the biodegradation of
treatmenttomicrobiallyoxidizedissolvedorganicmatterinthe the test material by following the decrease in oxygen in the
effluent. respirometer.
3.1.3 aerobic, adj—(a) taking place in the presence of
4.2 The test material is the sole source of carbon and energy
oxygen; (b) living or active in the presence of oxygen.
inthemedium.Areferencemateri
...
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